RchiOBHm_Chr2g0126411

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001654
Physical Location & Seq
Reverse (-)
40799249 .. 40801413
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49840

Sequence Viewer

Length: 1023 bp
ATGGCGTACACGAAGTTCTATCATGTCCTCTCCTTCTACCTCGCAACCCTAATAGCCATATACGAATTCCTTCTTTCAGTCTTGCCTTATATTTTGAATCCTGCTTCCATCCTAATCGCTTTTGCAGATGCATTTTTAGGAGTATACTTCAGATTGTGTGGTCTTTCTTCTTGTACTATTGAATTAGATGAACAAACCACCATGCATTTTTGGGCTTGCAATCCTAGAAGATCTGACAAGCCTGATCTACTGATGCTTCACGGGTATGGTGGCAATTCCAAGTGGCAATTCATGCACCAAGTCGGCTCACTGTCCCGGACTTTTAATTTGTACGTGCCCGATTTACTCTTCTTTGGCAAGTCATACACAAAAATGTCCGACAGGACGGAGGTGTTCCAAGCGAAATGTGTAGCGGAAGGGTTGAGAAGACTAGGCGTTGACAGGTTTGGTGTGTACTCTATAAGCTATGGAGGGTTTGTGGCATATTGGCTGGCGGAGATGTATCCGCAGGCAGTGGAGAGGCTTGTGATTGTGAGCTGTGGGATAGGGATGACAGAGGAGCAGAAAAAGGATCAGCTCAACAAGATTGGACGCAATGCATTGAAGCTCCTTGTACCTGAAAGTGCTCATGATCTAAGGGGCTTGGTGAATCTTTCGGTGCACAAACCCAGTCCTGTTAGGTGGCTACCCGATGTTTTCATCCGCGGGTTTATCCATGTGACTTATGAGAGAAACAGGAAGCAGAAACTAGAGATGGCAGAGCACTTGCTAAGGGAAAAAGCAGACCTAAACCTTCCCATTCTGACTCAGGAAACACTAATAATTTGGGGTGACAAGGATGTTGTCTTCCCAGTGAACTTGGCCTATGAGCTGCAAAGGTATTTGGGACCAAAATCAAATGTAGAGATAATCAAGGACACAGGTCATGCAGTGAATATGGATGCACCCATCTCCCTGAATACACTGATAACATCTTTTGTTTTGGGTTACTATCAATGTGATAGAATTGAACTATCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

340

Amino Acids

38.9

Weight (kDa)

8.11

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 83 - 180 1.2e-12 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 83 - 316 5.8e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AccII CGCG 1 cut(s) 705
AciI CCGC 5 cut(s) 413, 494, 506, 703, 705
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 133
AfaI GTAC 5 cut(s) 8, 175, 332, 455, 615
AgsI TTSAA 4 cut(s) 97, 182, 604, 1010
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 5 cut(s) 465, 537, 577, 607, 871
AluI AGCT 5 cut(s) 465, 537, 577, 607, 871
Alw21I GWGCWC 3 cut(s) 628, 663, 765
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 1 cut(s) 579
AoxI GGCC 1 cut(s) 861
ApaLI GTGCAC 1 cut(s) 659
ApeKI GCWGC 1 cut(s) 871
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
Asp700I GAANNNNTTC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 887
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 2 cut(s) 658, 842
AvaII GGWCC 1 cut(s) 887
BaeGI GKGCMC 2 cut(s) 339, 663
BbsI GAAGAC 2 cut(s) 433, 838
Bbv12I GWGCWC 3 cut(s) 628, 663, 765
BbvI GCAGC 1 cut(s) 858
BccI CCATC 3 cut(s) 116, 748, 956
BciVI GTATCC 1 cut(s) 513
BcnI CCSGG 1 cut(s) 316
BfaI CTAG 3 cut(s) 225, 431, 749
BfuI GTATCC 1 cut(s) 513
BglII AGATCT 1 cut(s) 230
BisI GCNGC 1 cut(s) 872
BlsI GCNGC 1 cut(s) 873
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 1 cut(s) 888
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 2 cut(s) 663, 845
BmsI GCATC 3 cut(s) 118, 243, 931
BmuI ACTGGG 2 cut(s) 663, 845
BoxI GACNNNNGTC 1 cut(s) 921
BpiI GAAGAC 2 cut(s) 433, 838
Bpu10I CCTNAGC 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 316
BsaAI YACGTR 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse1I ACTGG 2 cut(s) 669, 851
Bse3DI GCAATG 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 703
BseGI GGATG 5 cut(s) 108, 555, 699, 844, 946
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 2 cut(s) 669, 851
BseRI GAGGAG 1 cut(s) 572
BseSI GKGCMC 2 cut(s) 339, 663
BseXI GCAGC 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 705
BshFI GGCC 1 cut(s) 863
BsiHKAI GWGCWC 3 cut(s) 628, 663, 765
BsiSI CCGG 1 cut(s) 316
BslFI GGGAC 2 cut(s) 298, 900
BsmFI GGGAC 2 cut(s) 298, 900
BsnI GGCC 1 cut(s) 863
Bsp1286I GDGCHC 4 cut(s) 339, 628, 663, 765
Bsp143I GATC 4 cut(s) 230, 244, 571, 631
BspACI CCGC 5 cut(s) 413, 494, 506, 703, 705
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 1 cut(s) 820
BspFNI CGCG 1 cut(s) 705
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 1 cut(s) 888
BspPI GGATC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 601
BsrI ACTGG 2 cut(s) 669, 851
BssECI CCNNGG 1 cut(s) 703
BssMI GATC 4 cut(s) 230, 244, 571, 631
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 3 cut(s) 217, 492, 510
BstDEI CTNAG 3 cut(s) 635, 770, 807
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 5 cut(s) 108, 555, 699, 844, 946
BstFNI CGCG 1 cut(s) 705
BstKTI GATC 4 cut(s) 233, 247, 574, 634
BstMBI GATC 4 cut(s) 230, 244, 571, 631
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstPAI GACNNNNGTC 1 cut(s) 921
BstSCI CCNGG 1 cut(s) 314
BstSLI GKGCMC 2 cut(s) 339, 663
BstUI CGCG 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 858
BstV2I GAAGAC 2 cut(s) 433, 838
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BstZ17I GTATAC 1 cut(s) 145
BsuI GTATCC 1 cut(s) 513
BsuRI GGCC 1 cut(s) 863
BtgI CCRYGG 1 cut(s) 703
BtsCI GGATG 5 cut(s) 108, 555, 699, 844, 946
BtsI GCAGTG 2 cut(s) 519, 936
BtsIMutI CAGTG 5 cut(s) 308, 519, 858, 936, 962
Cac8I GCNNGC 3 cut(s) 217, 492, 510
CciI TCATGA 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 887
Cfr42I CCGCGG 1 cut(s) 706
CseI GACGC 1 cut(s) 600
Csp6I GTAC 5 cut(s) 7, 174, 331, 454, 614
CviAII CATG 6 cut(s) 23, 202, 292, 629, 716, 926
CviQI GTAC 5 cut(s) 7, 174, 331, 454, 614
DdeI CTNAG 3 cut(s) 635, 770, 807
DpnI GATC 4 cut(s) 232, 246, 573, 633
DpnII GATC 4 cut(s) 230, 244, 571, 631
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 65
EcoT22I ATGCAT 3 cut(s) 133, 207, 601
FaeI CATG 6 cut(s) 26, 205, 295, 632, 719, 929
FaqI GGGAC 2 cut(s) 298, 900
FatI CATG 6 cut(s) 22, 201, 291, 628, 715, 925
FauI CCCGC 1 cut(s) 698
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 872
FokI GGATG 5 cut(s) 95, 562, 686, 851, 953
Fsp4HI GCNGC 1 cut(s) 872
FspBI CTAG 3 cut(s) 225, 431, 749
GluI GCNGC 1 cut(s) 872
HaeIII GGCC 1 cut(s) 863
HapII CCGG 1 cut(s) 316
HgaI GACGC 1 cut(s) 600
Hin1II CATG 6 cut(s) 26, 205, 295, 632, 719, 929
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 3 cut(s) 97, 649, 805
HpaII CCGG 1 cut(s) 316
HphI GGTGA 2 cut(s) 658, 842
Hpy166II GTNNAC 6 cut(s) 9, 145, 439, 454, 661, 856
Hpy188I TCNGA 4 cut(s) 152, 235, 379, 804
Hpy188III TCNNGA 2 cut(s) 629, 809
Hpy8I GTNNAC 6 cut(s) 9, 145, 439, 454, 661, 856
HpyAV CCTTC 4 cut(s) 43, 80, 410, 803
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4IV ACGT 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 3 cut(s) 635, 770, 807
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 6 cut(s) 26, 205, 295, 632, 719, 929
KspI CCGCGG 1 cut(s) 706
Kzo9I GATC 4 cut(s) 230, 244, 571, 631
LmnI GCTCC 2 cut(s) 559, 612
Lsp1109I GCAGC 1 cut(s) 858
LweI GCATC 3 cut(s) 118, 243, 931
MaeI CTAG 3 cut(s) 225, 431, 749
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 718, 830, 986
MalI GATC 4 cut(s) 232, 246, 573, 633
MboI GATC 4 cut(s) 230, 244, 571, 631
MboII GAAGA 5 cut(s) 159, 240, 340, 438, 838
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 4 cut(s) 339, 628, 663, 765
MluCI AATT 7 cut(s) 65, 182, 274, 287, 325, 822, 1005
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 6 cut(s) 38, 50, 382, 464, 513, 550
Mph1103I ATGCAT 3 cut(s) 133, 207, 601
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 264, 371
MspA1I CMGCKG 1 cut(s) 705
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 705
MwoI GCNNNNNNNGC 1 cut(s) 292
NciI CCSGG 1 cut(s) 316
NdeII GATC 4 cut(s) 230, 244, 571, 631
NlaIII CATG 6 cut(s) 26, 205, 295, 632, 719, 929
NlaIV GGNNCC 1 cut(s) 888
NmuCI GTSAC 2 cut(s) 718, 830
NsiI ATGCAT 3 cut(s) 133, 207, 601
PagI TCATGA 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 97, 649
PfoI TCCNGGA 1 cut(s) 314
PkrI GCNGC 1 cut(s) 873
PleI GAGTC 1 cut(s) 799
PpsI GAGTC 1 cut(s) 799
Ppu21I YACGTR 1 cut(s) 334
PshAI GACNNNNGTC 1 cut(s) 921
PspN4I GGNNCC 1 cut(s) 888
PspPI GGNCC 1 cut(s) 887
PsrI GAACNNNNNNTAC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 5 cut(s) 8, 175, 332, 455, 615
RsaNI GTAC 5 cut(s) 7, 174, 331, 454, 614
RseI CAYNNNNRTG 2 cut(s) 264, 371
SacII CCGCGG 1 cut(s) 706
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 1 cut(s) 872
Sau3AI GATC 4 cut(s) 230, 244, 571, 631
Sau96I GGNCC 1 cut(s) 887
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 4 cut(s) 339, 628, 663, 765
SfaNI GCATC 3 cut(s) 118, 243, 931
Sfr303I CCGCGG 1 cut(s) 706
SgrBI CCGCGG 1 cut(s) 706
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 2 cut(s) 264, 371
Sse9I AATT 7 cut(s) 65, 182, 274, 287, 325, 822, 1005
SsiI CCGC 5 cut(s) 413, 494, 506, 703, 705
SspMI CTAG 3 cut(s) 225, 431, 749
StyD4I CCNGG 1 cut(s) 314
TaaI ACNGT 1 cut(s) 312
TaiI ACGT 1 cut(s) 336
TasI AATT 7 cut(s) 65, 182, 274, 287, 325, 822, 1005
TatI WGTACW 2 cut(s) 173, 453
TfiI GAWTC 2 cut(s) 97, 649
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 5 cut(s) 315, 519, 858, 936, 969
TseFI GTSAC 2 cut(s) 718, 830
TseI GCWGC 1 cut(s) 871
Tsp45I GTSAC 2 cut(s) 718, 830
TspDTI ATGAA 3 cut(s) 204, 280, 688
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 5 cut(s) 315, 519, 858, 936, 969
VneI GTGCAC 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 3 cut(s) 225, 431, 749
Zsp2I ATGCAT 3 cut(s) 133, 207, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.