Rh2BG459200

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001654
Physical Location & Seq
Reverse (-)
64953077 .. 64956256
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG459200.1

Sequence Viewer

Length: 822 bp
ATGGCGTACACGAAGTTCTATCATGTCCTCTCCTTCTACCTCGCAACCCTAATAGCCATATACGAATTCCTTCTTTCAGTCTTGCCTTATATTTTGAATCCTGCTTCCATCCTAATTGCTTTTGCAGATGCATTTTTAGGAGTATACTTCAGATTGTGTGGTCTTTCTTCATGTACTATTGAATTAGATGAACAAACCACCATGCATTTTTGGGCTTCCAATCCTAGAAGATTTGACAAGCCTGATCTACTGATGCTTCATGGGTATGGTGGCAATTCCAAGTGGCAATTCATGCACCAAGTCGGCTCACTCTCCCGGACTTTTAATTTGTACGTGCCCGATTTACTCTTCTTTGGCAAGTCATACACAAAAATGTCCGACAGGACAGAGGTGTTCCAAGCAAAATGTGTAGCGGAAGGGTTGAGGAGACTAGGCGTTGACAGGTTTGGTGTGTACTCTATAAGCTATGGAGGGTTTGTGGCATATTGGCTGGCGGAGATGTATCCACAGGCGGTGGAGAAGCTTGTGATTGTGAGTTGTGGGATAGGGATGACAGAGGAGCAGAAAAAGGATCAGCTCAACAAGATTGGACGCAATGCATTGAAGCTCCTTGTACCTGAAAGTGCTCATGATCTAAGGGGCTTGGTGAATCTTTCGGTGCACAAACCCAGTCCTGTTAGGTGGCTACCCGATGTTTTCATCCGTGGGTTTATCCATGTGACTTATGAGAGAAACAGGAAGCAGAAACTAGAGATGGCAGAGCACTTGCTAAGGGAAAAAGCAGACCTAAACCTTCCCATTCTGACTCAGGTAATTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

273

Amino Acids

31.28

Weight (kDa)

8.92

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 83 - 180 7.4e-13 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AciI CCGC 3 cut(s) 413, 494, 512
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 133
AfaI GTAC 5 cut(s) 8, 175, 332, 455, 615
AgsI TTSAA 3 cut(s) 97, 182, 604
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 4 cut(s) 465, 523, 577, 607
AluI AGCT 4 cut(s) 465, 523, 577, 607
Alw21I GWGCWC 3 cut(s) 628, 663, 765
Alw26I GTCTC 1 cut(s) 421
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 1 cut(s) 579
ApaLI GTGCAC 1 cut(s) 659
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
Asp700I GAANNNNTTC 1 cut(s) 69
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 658
BaeGI GKGCMC 2 cut(s) 339, 663
Bbv12I GWGCWC 3 cut(s) 628, 663, 765
BccI CCATC 2 cut(s) 116, 748
BciVI GTATCC 1 cut(s) 513
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 3 cut(s) 225, 431, 749
BfuI GTATCC 1 cut(s) 513
Bme1390I CCNGG 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 663
BmsI GCATC 2 cut(s) 118, 243
BmuI ACTGGG 1 cut(s) 663
Bpu10I CCTNAGC 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 316
BsaAI YACGTR 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 703
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse1I ACTGG 1 cut(s) 669
Bse3DI GCAATG 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 703
BseGI GGATG 3 cut(s) 108, 555, 699
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 2 cut(s) 439, 572
BseSI GKGCMC 2 cut(s) 339, 663
BsiHKAI GWGCWC 3 cut(s) 628, 663, 765
BsiSI CCGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 421
Bsp1286I GDGCHC 4 cut(s) 339, 628, 663, 765
Bsp143I GATC 3 cut(s) 244, 571, 631
BspACI CCGC 3 cut(s) 413, 494, 512
BspCNI CTCAG 1 cut(s) 820
BspHI TCATGA 1 cut(s) 628
BspPI GGATC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 601
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 1 cut(s) 703
BssMI GATC 3 cut(s) 244, 571, 631
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 3 cut(s) 635, 770, 807
BstDSI CCRYGG 1 cut(s) 703
BstF5I GGATG 3 cut(s) 108, 555, 699
BstKTI GATC 3 cut(s) 247, 574, 634
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 3 cut(s) 244, 571, 631
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 314
BstSLI GKGCMC 2 cut(s) 339, 663
BstZ17I GTATAC 1 cut(s) 145
BsuI GTATCC 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 703
BtsCI GGATG 3 cut(s) 108, 555, 699
Cac8I GCNNGC 1 cut(s) 492
CciI TCATGA 1 cut(s) 628
CseI GACGC 1 cut(s) 600
Csp6I GTAC 5 cut(s) 7, 174, 331, 454, 614
CviAII CATG 7 cut(s) 23, 171, 202, 260, 292, 629, 716
CviQI GTAC 5 cut(s) 7, 174, 331, 454, 614
DdeI CTNAG 3 cut(s) 635, 770, 807
DpnI GATC 3 cut(s) 246, 573, 633
DpnII GATC 3 cut(s) 244, 571, 631
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 65
EcoT22I ATGCAT 3 cut(s) 133, 207, 601
FaeI CATG 7 cut(s) 26, 174, 205, 263, 295, 632, 719
FatI CATG 7 cut(s) 22, 170, 201, 259, 291, 628, 715
FblI GTMKAC 1 cut(s) 144
FokI GGATG 3 cut(s) 95, 562, 686
FspBI CTAG 3 cut(s) 225, 431, 749
HapII CCGG 1 cut(s) 316
HgaI GACGC 1 cut(s) 600
Hin1II CATG 7 cut(s) 26, 174, 205, 263, 295, 632, 719
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 97, 649, 805
HpaII CCGG 1 cut(s) 316
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 5 cut(s) 9, 145, 439, 454, 661
Hpy188I TCNGA 3 cut(s) 152, 379, 804
Hpy188III TCNNGA 1 cut(s) 629
Hpy8I GTNNAC 5 cut(s) 9, 145, 439, 454, 661
HpyAV CCTTC 4 cut(s) 43, 80, 410, 803
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 6 cut(s) 125, 131, 205, 295, 599, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 3 cut(s) 635, 770, 807
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 7 cut(s) 26, 174, 205, 263, 295, 632, 719
Kzo9I GATC 3 cut(s) 244, 571, 631
LmnI GCTCC 2 cut(s) 559, 612
LweI GCATC 2 cut(s) 118, 243
MaeI CTAG 3 cut(s) 225, 431, 749
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 718
MalI GATC 3 cut(s) 246, 573, 633
MboI GATC 3 cut(s) 244, 571, 631
MboII GAAGA 3 cut(s) 159, 240, 340
MhlI GDGCHC 4 cut(s) 339, 628, 663, 765
MluCI AATT 7 cut(s) 65, 114, 182, 274, 287, 325, 813
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 6 cut(s) 38, 50, 382, 417, 464, 550
Mph1103I ATGCAT 3 cut(s) 133, 207, 601
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 2 cut(s) 324, 820
MslI CAYNNNNRTG 2 cut(s) 264, 371
MspI CCGG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 316
MwoI GCNNNNNNNGC 1 cut(s) 292
NciI CCSGG 1 cut(s) 316
NdeII GATC 3 cut(s) 244, 571, 631
NlaIII CATG 7 cut(s) 26, 174, 205, 263, 295, 632, 719
NmuCI GTSAC 1 cut(s) 718
NsiI ATGCAT 3 cut(s) 133, 207, 601
PagI TCATGA 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 97, 649
PfoI TCCNGGA 1 cut(s) 314
PleI GAGTC 1 cut(s) 799
PpsI GAGTC 1 cut(s) 799
Ppu21I YACGTR 1 cut(s) 334
PsrI GAACNNNNNNTAC 1 cut(s) 31
RsaI GTAC 5 cut(s) 8, 175, 332, 455, 615
RsaNI GTAC 5 cut(s) 7, 174, 331, 454, 614
RseI CAYNNNNRTG 2 cut(s) 264, 371
SaqAI TTAA 2 cut(s) 324, 820
Sau3AI GATC 3 cut(s) 244, 571, 631
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 4 cut(s) 339, 628, 663, 765
SfaNI GCATC 2 cut(s) 118, 243
SmiMI CAYNNNNRTG 2 cut(s) 264, 371
Sse9I AATT 7 cut(s) 65, 114, 182, 274, 287, 325, 813
SsiI CCGC 3 cut(s) 413, 494, 512
SspMI CTAG 3 cut(s) 225, 431, 749
StyD4I CCNGG 1 cut(s) 314
TaiI ACGT 1 cut(s) 336
TasI AATT 7 cut(s) 65, 114, 182, 274, 287, 325, 813
TatI WGTACW 2 cut(s) 173, 453
TfiI GAWTC 2 cut(s) 97, 649
Tru1I TTAA 2 cut(s) 324, 820
Tru9I TTAA 2 cut(s) 324, 820
TseFI GTSAC 1 cut(s) 718
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 5 cut(s) 159, 204, 248, 280, 688
TspGWI ACGGA 1 cut(s) 692
VneI GTGCAC 1 cut(s) 659
XapI RAATTY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 3 cut(s) 225, 431, 749
Zsp2I ATGCAT 3 cut(s) 133, 207, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.