Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 48 |
| AccII | CGCG | 1 cut(s) | 88 |
| AccIII | TCCGGA | 1 cut(s) | 502 |
| AciI | CCGC | 8 cut(s) | 37, 66, 88, 213, 223, 267, 468, 484 |
| AcuI | CTGAAG | 1 cut(s) | 440 |
| AfaI | GTAC | 6 cut(s) | 143, 329, 443, 452, 482, 736 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 30, 312, 445 |
| AflIII | ACRYGT | 1 cut(s) | 324 |
| AgeI | ACCGGT | 2 cut(s) | 369, 438 |
| AgsI | TTSAA | 5 cut(s) | 73, 322, 395, 673, 698 |
| AjnI | CCWGG | 1 cut(s) | 311 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 329, 361 |
| AluBI | AGCT | 3 cut(s) | 78, 462, 703 |
| AluI | AGCT | 3 cut(s) | 78, 462, 703 |
| Alw26I | GTCTC | 2 cut(s) | 22, 500 |
| Aor13HI | TCCGGA | 1 cut(s) | 502 |
| AoxI | GGCC | 1 cut(s) | 21 |
| ApeKI | GCWGC | 1 cut(s) | 34 |
| AsiGI | ACCGGT | 2 cut(s) | 369, 438 |
| AspLEI | GCGC | 2 cut(s) | 49, 88 |
| AspS9I | GGNCC | 4 cut(s) | 21, 210, 376, 436 |
| AsuHPI | GGTGA | 1 cut(s) | 724 |
| AsuNHI | GCTAGC | 1 cut(s) | 116 |
| AvaII | GGWCC | 3 cut(s) | 210, 376, 436 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 117, 150 |
| BbvI | GCAGC | 1 cut(s) | 21 |
| BceAI | ACGGC | 4 cut(s) | 8, 47, 274, 481 |
| BciT130I | CCWGG | 1 cut(s) | 313 |
| BciVI | GTATCC | 1 cut(s) | 145 |
| BclI | TGATCA | 1 cut(s) | 553 |
| BcoDI | GTCTC | 2 cut(s) | 22, 500 |
| BfaI | CTAG | 1 cut(s) | 117 |
| BfmI | CTRYAG | 1 cut(s) | 145 |
| BfuI | GTATCC | 1 cut(s) | 145 |
| BisI | GCNGC | 5 cut(s) | 35, 67, 89, 268, 469 |
| BlsI | GCNGC | 5 cut(s) | 36, 68, 90, 269, 470 |
| Bme1390I | CCNGG | 1 cut(s) | 313 |
| Bme18I | GGWCC | 3 cut(s) | 210, 376, 436 |
| BmgT120I | GGNCC | 4 cut(s) | 21, 210, 376, 436 |
| BmiI | GGNNCC | 1 cut(s) | 681 |
| BmrFI | CCNGG | 1 cut(s) | 313 |
| BmrI | ACTGGG | 1 cut(s) | 234 |
| BmsI | GCATC | 1 cut(s) | 310 |
| BmtI | GCTAGC | 1 cut(s) | 120 |
| BmuI | ACTGGG | 1 cut(s) | 234 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 710, 742 |
| Bpu10I | CCTNAGC | 2 cut(s) | 92, 104 |
| Bsa29I | ATCGAT | 1 cut(s) | 650 |
| BsaAI | YACGTR | 1 cut(s) | 331 |
| BsaWI | WCCGGW | 4 cut(s) | 369, 378, 438, 502 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 557, 587 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 30, 312, 445 |
| Bse118I | RCCGGY | 3 cut(s) | 225, 369, 438 |
| Bse1I | ACTGG | 1 cut(s) | 240 |
| BseAI | TCCGGA | 1 cut(s) | 502 |
| BseBI | CCWGG | 1 cut(s) | 313 |
| BseCI | ATCGAT | 1 cut(s) | 650 |
| BseGI | GGATG | 1 cut(s) | 301 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 30, 312, 445 |
| BseNI | ACTGG | 1 cut(s) | 240 |
| BseRI | GAGGAG | 3 cut(s) | 25, 28, 581 |
| BseXI | GCAGC | 1 cut(s) | 21 |
| BseYI | CCCAGC | 1 cut(s) | 427 |
| Bsh1236I | CGCG | 1 cut(s) | 88 |
| Bsh1285I | CGRYCG | 2 cut(s) | 374, 650 |
| BshFI | GGCC | 1 cut(s) | 23 |
| BshTI | ACCGGT | 2 cut(s) | 369, 438 |
| BshVI | ATCGAT | 1 cut(s) | 650 |
| BsiEI | CGRYCG | 2 cut(s) | 374, 650 |
| BsiSI | CCGG | 6 cut(s) | 226, 335, 370, 379, 439, 503 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 30, 312, 445 |
| BsmAI | GTCTC | 2 cut(s) | 22, 500 |
| BsmBI | CGTCTC | 1 cut(s) | 22 |
| BsnI | GGCC | 1 cut(s) | 23 |
| Bsp13I | TCCGGA | 1 cut(s) | 502 |
| Bsp143I | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| BspACI | CCGC | 8 cut(s) | 37, 66, 88, 213, 223, 267, 468, 484 |
| BspANI | GGCC | 1 cut(s) | 23 |
| BspDI | ATCGAT | 1 cut(s) | 650 |
| BspEI | TCCGGA | 1 cut(s) | 502 |
| BspFNI | CGCG | 1 cut(s) | 88 |
| BspLI | GGNNCC | 1 cut(s) | 681 |
| BspOI | GCTAGC | 1 cut(s) | 120 |
| BsrFI | RCCGGY | 3 cut(s) | 225, 369, 438 |
| BsrI | ACTGG | 1 cut(s) | 240 |
| BssAI | RCCGGY | 3 cut(s) | 225, 369, 438 |
| BssMI | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| Bst2UI | CCWGG | 1 cut(s) | 313 |
| Bst4CI | ACNGT | 6 cut(s) | 58, 175, 199, 455, 511, 633 |
| Bst6I | CTCTTC | 1 cut(s) | 416 |
| BstBAI | YACGTR | 1 cut(s) | 331 |
| BstC8I | GCNNGC | 2 cut(s) | 101, 118 |
| BstDEI | CTNAG | 2 cut(s) | 92, 104 |
| BstF5I | GGATG | 1 cut(s) | 301 |
| BstFNI | CGCG | 1 cut(s) | 88 |
| BstHHI | GCGC | 2 cut(s) | 49, 88 |
| BstKTI | GATC | 6 cut(s) | 160, 528, 556, 628, 650, 717 |
| BstMAI | GTCTC | 2 cut(s) | 22, 500 |
| BstMBI | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| BstMCI | CGRYCG | 2 cut(s) | 374, 650 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 75, 267, 340, 424, 468 |
| BstNI | CCWGG | 1 cut(s) | 313 |
| BstNSI | RCATGY | 1 cut(s) | 103 |
| BstSCI | CCNGG | 1 cut(s) | 311 |
| BstSFI | CTRYAG | 1 cut(s) | 145 |
| BstUI | CGCG | 1 cut(s) | 88 |
| BstV1I | GCAGC | 1 cut(s) | 21 |
| Bsu15I | ATCGAT | 1 cut(s) | 650 |
| BsuI | GTATCC | 1 cut(s) | 145 |
| BsuRI | GGCC | 1 cut(s) | 23 |
| BsuTUI | ATCGAT | 1 cut(s) | 650 |
| BtsCI | GGATG | 1 cut(s) | 301 |
| BtsIMutI | CAGTG | 1 cut(s) | 516 |
| Cac8I | GCNNGC | 2 cut(s) | 101, 118 |
| CfoI | GCGC | 2 cut(s) | 49, 88 |
| Cfr10I | RCCGGY | 3 cut(s) | 225, 369, 438 |
| Cfr13I | GGNCC | 4 cut(s) | 21, 210, 376, 436 |
| ClaI | ATCGAT | 1 cut(s) | 650 |
| CpoI | CGGWCCG | 1 cut(s) | 376 |
| Csp6I | GTAC | 6 cut(s) | 142, 328, 442, 451, 481, 735 |
| CspAI | ACCGGT | 2 cut(s) | 369, 438 |
| CspI | CGGWCCG | 1 cut(s) | 376 |
| CviAII | CATG | 4 cut(s) | 100, 121, 487, 623 |
| CviQI | GTAC | 6 cut(s) | 142, 328, 442, 451, 481, 735 |
| DdeI | CTNAG | 2 cut(s) | 92, 104 |
| DpnI | GATC | 6 cut(s) | 159, 527, 555, 627, 649, 716 |
| DpnII | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| Eam1104I | CTCTTC | 1 cut(s) | 416 |
| EarI | CTCTTC | 1 cut(s) | 416 |
| Eco47I | GGWCC | 3 cut(s) | 210, 376, 436 |
| Eco57I | CTGAAG | 1 cut(s) | 440 |
| EcoRII | CCWGG | 1 cut(s) | 311 |
| Esp3I | CGTCTC | 1 cut(s) | 22 |
| FaeI | CATG | 4 cut(s) | 103, 124, 490, 626 |
| FatI | CATG | 4 cut(s) | 99, 120, 486, 622 |
| FauI | CCCGC | 1 cut(s) | 30 |
| FbaI | TGATCA | 1 cut(s) | 553 |
| Fnu4HI | GCNGC | 5 cut(s) | 35, 67, 89, 268, 469 |
| FokI | GGATG | 1 cut(s) | 288 |
| Fsp4HI | GCNGC | 5 cut(s) | 35, 67, 89, 268, 469 |
| FspBI | CTAG | 1 cut(s) | 117 |
| FspI | TGCGCA | 1 cut(s) | 48 |
| GlaI | GCGC | 2 cut(s) | 48, 87 |
| GluI | GCNGC | 5 cut(s) | 35, 67, 89, 268, 469 |
| GsaI | CCCAGC | 1 cut(s) | 431 |
| HaeIII | GGCC | 1 cut(s) | 23 |
| HapII | CCGG | 6 cut(s) | 226, 335, 370, 379, 439, 503 |
| HhaI | GCGC | 2 cut(s) | 49, 88 |
| Hin1II | CATG | 4 cut(s) | 103, 124, 490, 626 |
| Hin6I | GCGC | 2 cut(s) | 47, 86 |
| HinP1I | GCGC | 2 cut(s) | 47, 86 |
| HincII | GTYRAC | 1 cut(s) | 178 |
| HindII | GTYRAC | 1 cut(s) | 178 |
| HindIII | AAGCTT | 1 cut(s) | 76 |
| HinfI | GANTC | 4 cut(s) | 247, 386, 601, 643 |
| HpaII | CCGG | 6 cut(s) | 226, 335, 370, 379, 439, 503 |
| HphI | GGTGA | 1 cut(s) | 724 |
| Hpy166II | GTNNAC | 7 cut(s) | 178, 202, 256, 328, 436, 451, 758 |
| Hpy188I | TCNGA | 2 cut(s) | 246, 376 |
| Hpy188III | TCNNGA | 5 cut(s) | 208, 352, 503, 529, 683 |
| Hpy8I | GTNNAC | 7 cut(s) | 178, 202, 256, 328, 436, 451, 758 |
| Hpy99I | CGWCG | 1 cut(s) | 188 |
| HpyAV | CCTTC | 4 cut(s) | 12, 511, 556, 704 |
| HpyCH4III | ACNGT | 6 cut(s) | 58, 175, 199, 455, 511, 633 |
| HpyCH4IV | ACGT | 2 cut(s) | 324, 330 |
| HpyCH4V | TGCA | 2 cut(s) | 171, 301 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 75, 267, 340, 424, 468 |
| HpyF3I | CTNAG | 2 cut(s) | 92, 104 |
| HpySE526I | ACGT | 2 cut(s) | 324, 330 |
| Hsp92II | CATG | 4 cut(s) | 103, 124, 490, 626 |
| HspAI | GCGC | 2 cut(s) | 47, 86 |
| Kpn2I | TCCGGA | 1 cut(s) | 502 |
| Ksp22I | TGATCA | 1 cut(s) | 553 |
| Kzo9I | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| LmnI | GCTCC | 2 cut(s) | 96, 381 |
| Lsp1109I | GCAGC | 1 cut(s) | 21 |
| LweI | GCATC | 1 cut(s) | 310 |
| MaeI | CTAG | 1 cut(s) | 117 |
| MaeII | ACGT | 2 cut(s) | 324, 330 |
| MalI | GATC | 6 cut(s) | 159, 527, 555, 627, 649, 716 |
| MboI | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| MboII | GAAGA | 4 cut(s) | 340, 433, 592, 715 |
| MlyI | GAGTC | 2 cut(s) | 395, 652 |
| MmeI | TCCRAC | 1 cut(s) | 354 |
| MnlI | CCTC | 8 cut(s) | 3, 6, 76, 406, 417, 559, 570, 703 |
| MroI | TCCGGA | 1 cut(s) | 502 |
| MseI | TTAA | 1 cut(s) | 763 |
| MslI | CAYNNNNRTG | 2 cut(s) | 261, 710 |
| MspI | CCGG | 6 cut(s) | 226, 335, 370, 379, 439, 503 |
| MspR9I | CCNGG | 1 cut(s) | 313 |
| MvaI | CCWGG | 1 cut(s) | 313 |
| MvnI | CGCG | 1 cut(s) | 88 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 75, 267, 340, 424, 468 |
| NdeII | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| NheI | GCTAGC | 1 cut(s) | 116 |
| NlaIII | CATG | 4 cut(s) | 103, 124, 490, 626 |
| NlaIV | GGNNCC | 1 cut(s) | 681 |
| NsbI | TGCGCA | 1 cut(s) | 48 |
| NspI | RCATGY | 1 cut(s) | 103 |
| PaeI | GCATGC | 1 cut(s) | 103 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 471 |
| PfeI | GAWTC | 2 cut(s) | 247, 601 |
| PflFI | GACNNNGTC | 1 cut(s) | 689 |
| PfoI | TCCNGGA | 1 cut(s) | 311 |
| PinAI | ACCGGT | 2 cut(s) | 369, 438 |
| PkrI | GCNGC | 5 cut(s) | 36, 68, 90, 269, 470 |
| Ple19I | CGATCG | 1 cut(s) | 650 |
| PleI | GAGTC | 2 cut(s) | 394, 651 |
| PpsI | GAGTC | 2 cut(s) | 394, 651 |
| Ppu21I | YACGTR | 1 cut(s) | 331 |
| Psp6I | CCWGG | 1 cut(s) | 311 |
| PspFI | CCCAGC | 1 cut(s) | 427 |
| PspGI | CCWGG | 1 cut(s) | 311 |
| PspN4I | GGNNCC | 1 cut(s) | 681 |
| PspPI | GGNCC | 4 cut(s) | 21, 210, 376, 436 |
| PsyI | GACNNNGTC | 1 cut(s) | 689 |
| PvuI | CGATCG | 1 cut(s) | 650 |
| RsaI | GTAC | 6 cut(s) | 143, 329, 443, 452, 482, 736 |
| RsaNI | GTAC | 6 cut(s) | 142, 328, 442, 451, 481, 735 |
| RseI | CAYNNNNRTG | 2 cut(s) | 261, 710 |
| Rsr2I | CGGWCCG | 1 cut(s) | 376 |
| RsrII | CGGWCCG | 1 cut(s) | 376 |
| SaqAI | TTAA | 1 cut(s) | 763 |
| SatI | GCNGC | 5 cut(s) | 35, 67, 89, 268, 469 |
| Sau3AI | GATC | 6 cut(s) | 157, 525, 553, 625, 647, 714 |
| Sau96I | GGNCC | 4 cut(s) | 21, 210, 376, 436 |
| SchI | GAGTC | 2 cut(s) | 395, 652 |
| ScrFI | CCNGG | 1 cut(s) | 313 |
| SetI | ASST | 8 cut(s) | 80, 327, 333, 464, 522, 705, 714, 757 |
| SfaNI | GCATC | 1 cut(s) | 310 |
| SfcI | CTRYAG | 1 cut(s) | 145 |
| SinI | GGWCC | 3 cut(s) | 210, 376, 436 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 261, 710 |
| SphI | GCATGC | 1 cut(s) | 103 |
| SsiI | CCGC | 8 cut(s) | 37, 66, 88, 213, 223, 267, 468, 484 |
| SspMI | CTAG | 1 cut(s) | 117 |
| StyD4I | CCNGG | 1 cut(s) | 311 |
| TaaI | ACNGT | 6 cut(s) | 58, 175, 199, 455, 511, 633 |
| TaiI | ACGT | 2 cut(s) | 327, 333 |
| TaqI | TCGA | 5 cut(s) | 183, 231, 646, 650, 717 |
| TatI | WGTACW | 1 cut(s) | 141 |
| TauI | GCSGC | 4 cut(s) | 69, 91, 270, 471 |
| TfiI | GAWTC | 2 cut(s) | 247, 601 |
| Tru1I | TTAA | 1 cut(s) | 763 |
| Tru9I | TTAA | 1 cut(s) | 763 |
| TscAI | CASTG | 1 cut(s) | 516 |
| TseI | GCWGC | 1 cut(s) | 34 |
| TspDTI | ATGAA | 2 cut(s) | 17, 137 |
| TspGWI | ACGGA | 2 cut(s) | 281, 722 |
| TspRI | CASTG | 1 cut(s) | 516 |
| Tth111I | GACNNNGTC | 1 cut(s) | 689 |
| VpaK11BI | GGWCC | 3 cut(s) | 210, 376, 436 |
| XceI | RCATGY | 1 cut(s) | 103 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 247, 406 |
| XspI | CTAG | 1 cut(s) | 117 |