Rroxscaffold_152G00434680

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0001654
Physical Location & Seq
Reverse (-)
177651 .. 179888
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434680.1

Sequence Viewer

Length: 1161 bp
ATGGTGGTCGGAATCGCCGAGAATTGCATAGCTCACCGAAGAAGTCTCGGAGCTTCTAGTCGTCGTTGCTCCTTCTCCGTTCAATGCATGAGTGATCCAAAGACACGAGACGTAGCTGATGCAGAGTCGAAGGTAATAGTGGCTGTGCGCGATCGTGAGGTGGCCGGATGTCATCCCTCTAGTCGGGTTTCTTTCTCGTGTCGCGGTGGTGTCCGGGTCGAGAGCATCGAGCTCTCTCTCTCTCTCGTCCGTGTTTTTGGTCTCCCAATCTCCTTGTTGGAGAGACCTTGGAGAATCCCAGATACACTTGTATCCCTATACTTCAGACTATGCGGTCTATCTCCTTGCACCGTTGACATTGACGACCAAACCACAGTCCACTTCTGGACCTCCAACCACCGCCGGTTCGATAAACCAAGCCTGGTTCTGGTTCACGGCTATGGCGGCAACTCCCTGTGGCAGTTCGTCCGTCAAGTGCATCCCCTGTCCAGGAAGTTCAACGTGTACGTGCCGGATTTGCTGTTCTTCGGGAAGTCTTACACGAACCGGTCGGACCGGAGCGAGTCTTTTCAAGCCAAGTGCGTTATGGAGGGGCTGAAGAGGCTGGGCGTGGACCGGTACTCGGTGTACGGTATTAGCTACGGCGGCTTCGTGGCGTACCGCATGGCAGAGATGTGTCCGGAGACAGTGGAGAAGGTTGTGATCGTGAGTAGTGGGGTGGTGTGGAATGATCAGCAGAAGGAGGAGTTGTTGAGGAAGAATTCAAACGCTTTGGAGATTCTTGTAGCGAATAATCCACATGATCTACGGTTGTTAGTGAATCGATCGATTTACAAGTATGATGTTTTCAAGTGGGTTCCTGACTATGTCCTTCAAAAGCTCATTGAGGCGAACAAGGAAAATAGAAAGGAAAAATTAGAGCTATTGGAACACTTGGTGGCAAAGAAAGCAGATCCAAGCCTTGCTATCCTAAGTCAGGAAACGCTAATAATTTGGGGTGATCAAGATAATGTCTTCCCACTTTACATGGCCTACCAGTTGCAAAGGCACTTGGGCCCCAAATCAAAACTAGAGATTATCAAGGACACAGGGCATGCAGCAAATTTTGATTCACCGGACACAATCAATGGATTGATAAAGTCATTTGTTTTGGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

386

Amino Acids

43.76

Weight (kDa)

8.85

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 139 - 372 8.6e-24 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 375 8.4e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 333
AccII CGCG 2 cut(s) 150, 204
AccIII TCCGGA 1 cut(s) 679
AciI CCGC 6 cut(s) 204, 333, 400, 444, 645, 661
AclWI GGATC 2 cut(s) 89, 947
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 2 cut(s) 760, 1102
AcuI CTGAAG 2 cut(s) 307, 617
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 4 cut(s) 506, 620, 629, 659
AfiI CCNNNNNNNGG 5 cut(s) 183, 427, 489, 622, 976
AflIII ACRYGT 1 cut(s) 501
AgeI ACCGGT 2 cut(s) 546, 615
AgsI TTSAA 6 cut(s) 83, 499, 572, 765, 850, 875
AjnI CCWGG 2 cut(s) 420, 488
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 7 cut(s) 32, 53, 116, 232, 639, 880, 922
AluI AGCT 7 cut(s) 32, 53, 116, 232, 639, 880, 922
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 5 cut(s) 50, 102, 266, 277, 677
AlwI GGATC 2 cut(s) 89, 947
Aor13HI TCCGGA 1 cut(s) 679
AoxI GGCC 3 cut(s) 162, 1029, 1054
ApaI GGGCCC 1 cut(s) 1058
ApeKI GCWGC 1 cut(s) 1097
ApoI RAATTY 2 cut(s) 760, 1102
AsiGI ACCGGT 2 cut(s) 546, 615
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 5 cut(s) 387, 553, 613, 1054, 1055
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 26, 1010, 1104
AvaII GGWCC 3 cut(s) 387, 553, 613
BaeGI GKGCMC 1 cut(s) 1058
BaeI ACNNNNGTAYC 4 cut(s) 294, 294, 327, 327
BanII GRGCYC 2 cut(s) 234, 1058
BauI CACGAG 2 cut(s) 105, 196
BbsI GAAGAC 1 cut(s) 1006
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 1109
BceAI ACGGC 2 cut(s) 451, 658
BciT130I CCWGG 2 cut(s) 422, 490
BciVI GTATCC 1 cut(s) 322
BclI TGATCA 2 cut(s) 730, 1000
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 5 cut(s) 50, 102, 266, 277, 677
BfaI CTAG 3 cut(s) 57, 180, 1070
BfuI GTATCC 1 cut(s) 322
BisI GCNGC 3 cut(s) 445, 646, 1098
BlsI GCNGC 3 cut(s) 446, 647, 1099
Bme1390I CCNGG 3 cut(s) 215, 422, 490
Bme18I GGWCC 3 cut(s) 387, 553, 613
BmgT120I GGNCC 5 cut(s) 387, 553, 613, 1054, 1055
BmiI GGNNCC 3 cut(s) 858, 1056, 1057
BmrFI CCNGG 3 cut(s) 215, 422, 490
BmsI GCATC 3 cut(s) 109, 234, 487
BpiI GAAGAC 1 cut(s) 1006
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 2 cut(s) 823, 827
BsaAI YACGTR 1 cut(s) 508
BsaI GGTCTC 2 cut(s) 266, 277
BsaJI CCNNGG 1 cut(s) 287
BsaWI WCCGGW 5 cut(s) 546, 555, 615, 679, 1114
BsaXI ACNNNNNCTCC 2 cut(s) 734, 764
Bsc4I CCNNNNNNNGG 5 cut(s) 183, 427, 489, 622, 976
Bse118I RCCGGY 3 cut(s) 402, 546, 615
Bse1I ACTGG 1 cut(s) 1036
BseAI TCCGGA 1 cut(s) 679
BseBI CCWGG 2 cut(s) 422, 490
BseCI ATCGAT 2 cut(s) 823, 827
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 3 cut(s) 172, 173, 478
BseLI CCNNNNNNNGG 5 cut(s) 183, 427, 489, 622, 976
BseNI ACTGG 1 cut(s) 1036
BseRI GAGGAG 1 cut(s) 758
BseSI GKGCMC 1 cut(s) 1058
BseXI GCAGC 1 cut(s) 1109
BseYI CCCAGC 1 cut(s) 604
Bsh1236I CGCG 2 cut(s) 150, 204
Bsh1285I CGRYCG 3 cut(s) 154, 551, 827
BshFI GGCC 3 cut(s) 164, 1031, 1056
BshTI ACCGGT 2 cut(s) 546, 615
BshVI ATCGAT 2 cut(s) 823, 827
BsiEI CGRYCG 3 cut(s) 154, 551, 827
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 9 cut(s) 165, 214, 403, 512, 547, 556, 616, 680, 1115
BslI CCNNNNNNNGG 5 cut(s) 183, 427, 489, 622, 976
BsmAI GTCTC 5 cut(s) 50, 102, 266, 277, 677
BsmBI CGTCTC 1 cut(s) 102
BsnI GGCC 3 cut(s) 164, 1031, 1056
Bso31I GGTCTC 2 cut(s) 266, 277
Bsp120I GGGCCC 1 cut(s) 1054
Bsp1286I GDGCHC 2 cut(s) 234, 1058
Bsp13I TCCGGA 1 cut(s) 679
Bsp143I GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
BspACI CCGC 6 cut(s) 204, 333, 400, 444, 645, 661
BspANI GGCC 3 cut(s) 164, 1031, 1056
BspDI ATCGAT 2 cut(s) 823, 827
BspEI TCCGGA 1 cut(s) 679
BspFNI CGCG 2 cut(s) 150, 204
BspLI GGNNCC 3 cut(s) 858, 1056, 1057
BspPI GGATC 2 cut(s) 89, 947
BspTNI GGTCTC 2 cut(s) 266, 277
BsrFI RCCGGY 3 cut(s) 402, 546, 615
BsrI ACTGG 1 cut(s) 1036
BssAI RCCGGY 3 cut(s) 402, 546, 615
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
BssSI CACGAG 2 cut(s) 105, 196
BssT1I CCWWGG 1 cut(s) 287
Bst2BI CACGAG 2 cut(s) 105, 196
Bst2UI CCWGG 2 cut(s) 422, 490
Bst4CI ACNGT 5 cut(s) 352, 376, 632, 688, 810
Bst6I CTCTTC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 508
BstC8I GCNNGC 1 cut(s) 1095
BstDEI CTNAG 1 cut(s) 971
BstENI CCTNNNNNAGG 1 cut(s) 974
BstF5I GGATG 3 cut(s) 172, 173, 478
BstFNI CGCG 2 cut(s) 150, 204
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 8 cut(s) 97, 154, 705, 733, 805, 827, 955, 1003
BstMAI GTCTC 5 cut(s) 50, 102, 266, 277, 677
BstMBI GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
BstMCI CGRYCG 3 cut(s) 154, 551, 827
BstMWI GCNNNNNNNGC 5 cut(s) 444, 517, 601, 645, 947
BstNI CCWGG 2 cut(s) 422, 490
BstNSI RCATGY 1 cut(s) 1097
BstSCI CCNGG 3 cut(s) 213, 420, 488
BstSLI GKGCMC 1 cut(s) 1058
BstUI CGCG 2 cut(s) 150, 204
BstV1I GCAGC 1 cut(s) 1109
BstV2I GAAGAC 1 cut(s) 1006
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
Bsu15I ATCGAT 2 cut(s) 823, 827
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 164, 1031, 1056
BsuTUI ATCGAT 2 cut(s) 823, 827
BtsCI GGATG 3 cut(s) 172, 173, 478
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 1095
CfoI GCGC 1 cut(s) 150
Cfr10I RCCGGY 3 cut(s) 402, 546, 615
Cfr13I GGNCC 5 cut(s) 387, 553, 613, 1054, 1055
ClaI ATCGAT 2 cut(s) 823, 827
CpoI CGGWCCG 1 cut(s) 553
Csp6I GTAC 4 cut(s) 505, 619, 628, 658
CspAI ACCGGT 2 cut(s) 546, 615
CspI CGGWCCG 1 cut(s) 553
CviAII CATG 5 cut(s) 88, 664, 800, 1027, 1094
CviQI GTAC 4 cut(s) 505, 619, 628, 658
DdeI CTNAG 1 cut(s) 971
DpnI GATC 8 cut(s) 96, 153, 704, 732, 804, 826, 954, 1002
DpnII GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
DraIII CACNNNGTG 1 cut(s) 937
DrdI GACNNNNNNGTC 1 cut(s) 333
DseDI GACNNNNNNGTC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 287
Eco24I GRGCYC 2 cut(s) 234, 1058
Eco31I GGTCTC 2 cut(s) 266, 277
Eco47I GGWCC 3 cut(s) 387, 553, 613
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 307, 617
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 974
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRI GAATTC 1 cut(s) 760
EcoRII CCWGG 2 cut(s) 420, 488
EcoT14I CCWWGG 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 2 cut(s) 234, 1058
ErhI CCWWGG 1 cut(s) 287
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 5 cut(s) 91, 667, 803, 1030, 1097
FatI CATG 5 cut(s) 87, 663, 799, 1026, 1093
FbaI TGATCA 2 cut(s) 730, 1000
Fnu4HI GCNGC 3 cut(s) 445, 646, 1098
FokI GGATG 3 cut(s) 159, 180, 465
FriOI GRGCYC 2 cut(s) 234, 1058
Fsp4HI GCNGC 3 cut(s) 445, 646, 1098
FspBI CTAG 3 cut(s) 57, 180, 1070
GlaI GCGC 1 cut(s) 149
GluI GCNGC 3 cut(s) 445, 646, 1098
GsaI CCCAGC 1 cut(s) 608
HaeIII GGCC 3 cut(s) 164, 1031, 1056
HapII CCGG 9 cut(s) 165, 214, 403, 512, 547, 556, 616, 680, 1115
HhaI GCGC 1 cut(s) 150
Hin1II CATG 5 cut(s) 91, 667, 803, 1030, 1097
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 7 cut(s) 12, 125, 294, 563, 778, 820, 1109
HpaII CCGG 9 cut(s) 165, 214, 403, 512, 547, 556, 616, 680, 1115
HphI GGTGA 3 cut(s) 26, 1010, 1104
Hpy166II GTNNAC 6 cut(s) 355, 379, 433, 505, 613, 628
Hpy188I TCNGA 4 cut(s) 11, 50, 326, 553
Hpy188III TCNNGA 9 cut(s) 155, 220, 385, 529, 680, 706, 860, 977, 1004
Hpy8I GTNNAC 6 cut(s) 355, 379, 433, 505, 613, 628
Hpy99I CGWCG 1 cut(s) 66
HpyAV CCTTC 5 cut(s) 82, 124, 688, 733, 881
HpyCH4III ACNGT 5 cut(s) 352, 376, 632, 688, 810
HpyCH4IV ACGT 3 cut(s) 111, 501, 507
HpyCH4V TGCA 7 cut(s) 27, 87, 122, 348, 478, 1042, 1097
HpyF10VI GCNNNNNNNGC 5 cut(s) 444, 517, 601, 645, 947
HpyF3I CTNAG 1 cut(s) 971
HpySE526I ACGT 3 cut(s) 111, 501, 507
Hsp92II CATG 5 cut(s) 91, 667, 803, 1030, 1097
HspAI GCGC 1 cut(s) 148
Kpn2I TCCGGA 1 cut(s) 679
Ksp22I TGATCA 2 cut(s) 730, 1000
Kzo9I GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
LmnI GCTCC 3 cut(s) 50, 74, 558
Lsp1109I GCAGC 1 cut(s) 1109
LweI GCATC 3 cut(s) 109, 234, 487
MaeI CTAG 3 cut(s) 57, 180, 1070
MaeII ACGT 3 cut(s) 111, 501, 507
MalI GATC 8 cut(s) 96, 153, 704, 732, 804, 826, 954, 1002
MboI GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
MboII GAAGA 5 cut(s) 51, 517, 610, 769, 1006
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 2 cut(s) 234, 1058
MluCI AATT 5 cut(s) 22, 760, 914, 990, 1102
MlyI GAGTC 2 cut(s) 134, 572
MmeI TCCRAC 3 cut(s) 258, 417, 531
MnlI CCTC 8 cut(s) 151, 187, 400, 583, 594, 736, 747, 880
Mph1103I ATGCAT 1 cut(s) 89
MroI TCCGGA 1 cut(s) 679
MseI TTAA 1 cut(s) 1159
MslI CAYNNNNRTG 1 cut(s) 438
MspI CCGG 9 cut(s) 165, 214, 403, 512, 547, 556, 616, 680, 1115
MspR9I CCNGG 3 cut(s) 215, 422, 490
MvaI CCWGG 2 cut(s) 422, 490
MvnI CGCG 2 cut(s) 150, 204
MwoI GCNNNNNNNGC 5 cut(s) 444, 517, 601, 645, 947
NciI CCSGG 1 cut(s) 215
NdeII GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
NlaIII CATG 5 cut(s) 91, 667, 803, 1030, 1097
NlaIV GGNNCC 3 cut(s) 858, 1056, 1057
NmeAIII GCCGAG 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 1 cut(s) 1097
PaeI GCATGC 1 cut(s) 1097
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 225, 648
PfeI GAWTC 5 cut(s) 12, 294, 778, 820, 1109
PflFI GACNNNGTC 1 cut(s) 866
PfoI TCCNGGA 1 cut(s) 488
PinAI ACCGGT 2 cut(s) 546, 615
PkrI GCNGC 3 cut(s) 446, 647, 1099
Ple19I CGATCG 2 cut(s) 154, 827
PleI GAGTC 2 cut(s) 133, 571
PpsI GAGTC 2 cut(s) 133, 571
Ppu21I YACGTR 1 cut(s) 508
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 420, 488
PspFI CCCAGC 1 cut(s) 604
PspGI CCWGG 2 cut(s) 420, 488
PspN4I GGNNCC 3 cut(s) 858, 1056, 1057
PspOMI GGGCCC 1 cut(s) 1054
PspPI GGNCC 5 cut(s) 387, 553, 613, 1054, 1055
PsuI RGATCY 1 cut(s) 952
PsyI GACNNNGTC 1 cut(s) 866
PvuI CGATCG 2 cut(s) 154, 827
RsaI GTAC 4 cut(s) 506, 620, 629, 659
RsaNI GTAC 4 cut(s) 505, 619, 628, 658
RseI CAYNNNNRTG 1 cut(s) 438
Rsr2I CGGWCCG 1 cut(s) 553
RsrII CGGWCCG 1 cut(s) 553
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 1159
SatI GCNGC 3 cut(s) 445, 646, 1098
Sau3AI GATC 8 cut(s) 94, 151, 702, 730, 802, 824, 952, 1000
Sau96I GGNCC 5 cut(s) 387, 553, 613, 1054, 1055
SchI GAGTC 2 cut(s) 134, 572
ScrFI CCNGG 3 cut(s) 215, 422, 490
SduI GDGCHC 2 cut(s) 234, 1058
SfaNI GCATC 3 cut(s) 109, 234, 487
SinI GGWCC 3 cut(s) 387, 553, 613
SmiMI CAYNNNNRTG 1 cut(s) 438
SphI GCATGC 1 cut(s) 1097
Sse9I AATT 5 cut(s) 22, 760, 914, 990, 1102
SsiI CCGC 6 cut(s) 204, 333, 400, 444, 645, 661
SspMI CTAG 3 cut(s) 57, 180, 1070
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 3 cut(s) 213, 420, 488
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 5 cut(s) 352, 376, 632, 688, 810
TaiI ACGT 3 cut(s) 114, 504, 510
TaqI TCGA 6 cut(s) 128, 219, 228, 408, 823, 827
TasI AATT 5 cut(s) 22, 760, 914, 990, 1102
TauI GCSGC 2 cut(s) 447, 648
TfiI GAWTC 5 cut(s) 12, 294, 778, 820, 1109
Tru1I TTAA 1 cut(s) 1159
Tru9I TTAA 1 cut(s) 1159
TscAI CASTG 1 cut(s) 693
TseI GCWGC 1 cut(s) 1097
TspGWI ACGGA 3 cut(s) 67, 239, 458
TspRI CASTG 1 cut(s) 693
Tth111I GACNNNGTC 1 cut(s) 866
VpaK11BI GGWCC 3 cut(s) 387, 553, 613
XagI CCTNNNNNAGG 1 cut(s) 974
XapI RAATTY 2 cut(s) 760, 1102
XceI RCATGY 1 cut(s) 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XspI CTAG 3 cut(s) 57, 180, 1070
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.