Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 302 |
| AccIII | TCCGGA | 1 cut(s) | 502 |
| AciI | CCGC | 1 cut(s) | 48 |
| AclWI | GGATC | 1 cut(s) | 108 |
| AcoI | YGGCCR | 1 cut(s) | 762 |
| AcsI | RAATTY | 1 cut(s) | 1002 |
| AdeI | CACNNNGTG | 1 cut(s) | 760 |
| AfaI | GTAC | 3 cut(s) | 172, 329, 656 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 222, 312, 385, 445, 799 |
| AgeI | ACCGGT | 3 cut(s) | 369, 438, 645 |
| AgsI | TTSAA | 7 cut(s) | 115, 322, 421, 577, 673, 752, 780 |
| AjnI | CCWGG | 1 cut(s) | 311 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 92, 124, 329, 361 |
| AluBI | AGCT | 5 cut(s) | 137, 462, 571, 713, 745 |
| AluI | AGCT | 5 cut(s) | 137, 462, 571, 713, 745 |
| Alw21I | GWGCWC | 1 cut(s) | 204 |
| Alw26I | GTCTC | 3 cut(s) | 164, 248, 500 |
| Alw44I | GTGCAC | 1 cut(s) | 200 |
| AlwI | GGATC | 1 cut(s) | 108 |
| Aor13HI | TCCGGA | 1 cut(s) | 502 |
| AoxI | GGCC | 2 cut(s) | 762, 852 |
| ApaLI | GTGCAC | 1 cut(s) | 200 |
| ApeKI | GCWGC | 1 cut(s) | 920 |
| ApoI | RAATTY | 1 cut(s) | 1002 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 819, 851 |
| AsiGI | ACCGGT | 3 cut(s) | 369, 438, 645 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 26 |
| AspLEI | GCGC | 1 cut(s) | 228 |
| AspS9I | GGNCC | 4 cut(s) | 376, 436, 853, 878 |
| AsuC2I | CCSGG | 1 cut(s) | 240 |
| AsuHPI | GGTGA | 2 cut(s) | 833, 927 |
| AvaII | GGWCC | 3 cut(s) | 376, 436, 878 |
| BaeGI | GKGCMC | 1 cut(s) | 204 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 117, 150 |
| BalI | TGGCCA | 1 cut(s) | 764 |
| BanI | GGYRCC | 1 cut(s) | 302 |
| BbsI | GAAGAC | 1 cut(s) | 829 |
| Bbv12I | GWGCWC | 1 cut(s) | 204 |
| BbvI | GCAGC | 1 cut(s) | 932 |
| BccI | CCATC | 3 cut(s) | 481, 864, 1003 |
| BciT130I | CCWGG | 1 cut(s) | 313 |
| BciVI | GTATCC | 1 cut(s) | 510 |
| BcnI | CCSGG | 1 cut(s) | 240 |
| BcoDI | GTCTC | 3 cut(s) | 164, 248, 500 |
| BfaI | CTAG | 1 cut(s) | 104 |
| BfoI | RGCGCY | 1 cut(s) | 229 |
| BfuI | GTATCC | 1 cut(s) | 510 |
| BisI | GCNGC | 1 cut(s) | 921 |
| BlsI | GCNGC | 1 cut(s) | 922 |
| Bme1390I | CCNGG | 2 cut(s) | 240, 313 |
| Bme18I | GGWCC | 3 cut(s) | 376, 436, 878 |
| BmgT120I | GGNCC | 4 cut(s) | 376, 436, 853, 878 |
| BmiI | GGNNCC | 4 cut(s) | 304, 681, 879, 880 |
| BmrFI | CCNGG | 2 cut(s) | 240, 313 |
| BmrI | ACTGGG | 1 cut(s) | 106 |
| BmsI | GCATC | 1 cut(s) | 59 |
| BmuI | ACTGGG | 1 cut(s) | 106 |
| BoxI | GACNNNNGTC | 1 cut(s) | 912 |
| BpiI | GAAGAC | 1 cut(s) | 829 |
| BpuMI | CCSGG | 1 cut(s) | 240 |
| BsaAI | YACGTR | 1 cut(s) | 331 |
| BsaBI | GATNNNNATC | 1 cut(s) | 981 |
| BsaI | GGTCTC | 2 cut(s) | 164, 248 |
| BsaJI | CCNNGG | 1 cut(s) | 694 |
| BsaWI | WCCGGW | 5 cut(s) | 369, 378, 438, 502, 645 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 222, 312, 385, 445, 799 |
| Bse118I | RCCGGY | 4 cut(s) | 222, 369, 438, 645 |
| Bse1I | ACTGG | 1 cut(s) | 101 |
| Bse8I | GATNNNNATC | 1 cut(s) | 981 |
| BseAI | TCCGGA | 1 cut(s) | 502 |
| BseBI | CCWGG | 1 cut(s) | 313 |
| BseDI | CCNNGG | 1 cut(s) | 694 |
| BseGI | GGATG | 3 cut(s) | 492, 559, 571 |
| BseJI | GATNNNNATC | 1 cut(s) | 981 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 222, 312, 385, 445, 799 |
| BseMII | CTCAG | 2 cut(s) | 449, 483 |
| BseNI | ACTGG | 1 cut(s) | 101 |
| BseSI | GKGCMC | 1 cut(s) | 204 |
| BseXI | GCAGC | 1 cut(s) | 932 |
| BshFI | GGCC | 2 cut(s) | 764, 854 |
| BshNI | GGYRCC | 1 cut(s) | 302 |
| BshTI | ACCGGT | 3 cut(s) | 369, 438, 645 |
| BsiHKAI | GWGCWC | 1 cut(s) | 204 |
| BsiSI | CCGG | 8 cut(s) | 223, 240, 335, 370, 379, 439, 503, 646 |
| BslFI | GGGAC | 1 cut(s) | 864 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 222, 312, 385, 445, 799 |
| BsmAI | GTCTC | 3 cut(s) | 164, 248, 500 |
| BsmBI | CGTCTC | 1 cut(s) | 500 |
| BsmFI | GGGAC | 1 cut(s) | 864 |
| BsmI | GAATGC | 1 cut(s) | 28 |
| BsnI | GGCC | 2 cut(s) | 764, 854 |
| Bso31I | GGTCTC | 2 cut(s) | 164, 248 |
| Bsp1286I | GDGCHC | 1 cut(s) | 204 |
| Bsp13I | TCCGGA | 1 cut(s) | 502 |
| Bsp1407I | TGTACA | 2 cut(s) | 170, 654 |
| Bsp143I | GATC | 2 cut(s) | 100, 625 |
| BspACI | CCGC | 1 cut(s) | 48 |
| BspANI | GGCC | 2 cut(s) | 764, 854 |
| BspCNI | CTCAG | 2 cut(s) | 450, 484 |
| BspEI | TCCGGA | 1 cut(s) | 502 |
| BspLI | GGNNCC | 4 cut(s) | 304, 681, 879, 880 |
| BspPI | GGATC | 1 cut(s) | 108 |
| BspT107I | GGYRCC | 1 cut(s) | 302 |
| BspTNI | GGTCTC | 2 cut(s) | 164, 248 |
| BsrFI | RCCGGY | 4 cut(s) | 222, 369, 438, 645 |
| BsrGI | TGTACA | 2 cut(s) | 170, 654 |
| BsrI | ACTGG | 1 cut(s) | 101 |
| BssAI | RCCGGY | 4 cut(s) | 222, 369, 438, 645 |
| BssECI | CCNNGG | 1 cut(s) | 694 |
| BssMI | GATC | 2 cut(s) | 100, 625 |
| Bst2UI | CCWGG | 1 cut(s) | 313 |
| Bst4CI | ACNGT | 6 cut(s) | 199, 455, 467, 511, 633, 946 |
| Bst6I | CTCTTC | 1 cut(s) | 416 |
| BstAUI | TGTACA | 2 cut(s) | 170, 654 |
| BstBAI | YACGTR | 1 cut(s) | 331 |
| BstDEI | CTNAG | 2 cut(s) | 458, 492 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 310, 797 |
| BstF5I | GGATG | 3 cut(s) | 492, 559, 571 |
| BstH2I | RGCGCY | 1 cut(s) | 229 |
| BstHHI | GCGC | 1 cut(s) | 228 |
| BstKTI | GATC | 2 cut(s) | 103, 628 |
| BstMAI | GTCTC | 3 cut(s) | 164, 248, 500 |
| BstMBI | GATC | 2 cut(s) | 100, 625 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 267, 340 |
| BstNI | CCWGG | 1 cut(s) | 313 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 912 |
| BstSCI | CCNGG | 2 cut(s) | 238, 311 |
| BstSLI | GKGCMC | 1 cut(s) | 204 |
| BstV1I | GCAGC | 1 cut(s) | 932 |
| BstV2I | GAAGAC | 1 cut(s) | 829 |
| BstX2I | RGATCY | 1 cut(s) | 100 |
| BstYI | RGATCY | 1 cut(s) | 100 |
| BsuI | GTATCC | 1 cut(s) | 510 |
| BsuRI | GGCC | 2 cut(s) | 764, 854 |
| BtsCI | GGATG | 3 cut(s) | 492, 559, 571 |
| CfoI | GCGC | 1 cut(s) | 228 |
| Cfr10I | RCCGGY | 4 cut(s) | 222, 369, 438, 645 |
| Cfr13I | GGNCC | 4 cut(s) | 376, 436, 853, 878 |
| Csp6I | GTAC | 3 cut(s) | 171, 328, 655 |
| CspAI | ACCGGT | 3 cut(s) | 369, 438, 645 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 34 |
| CviAII | CATG | 5 cut(s) | 257, 296, 623, 662, 917 |
| CviQI | GTAC | 3 cut(s) | 171, 328, 655 |
| DdeI | CTNAG | 2 cut(s) | 458, 492 |
| DpnI | GATC | 2 cut(s) | 102, 627 |
| DpnII | GATC | 2 cut(s) | 100, 625 |
| DraIII | CACNNNGTG | 1 cut(s) | 760 |
| EaeI | YGGCCR | 1 cut(s) | 762 |
| Eam1104I | CTCTTC | 1 cut(s) | 416 |
| EarI | CTCTTC | 1 cut(s) | 416 |
| Eco31I | GGTCTC | 2 cut(s) | 164, 248 |
| Eco47I | GGWCC | 3 cut(s) | 376, 436, 878 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 310, 797 |
| EcoO109I | RGGNCCY | 1 cut(s) | 878 |
| EcoRI | GAATTC | 1 cut(s) | 1002 |
| EcoRII | CCWGG | 1 cut(s) | 311 |
| EcoT22I | ATGCAT | 1 cut(s) | 955 |
| Esp3I | CGTCTC | 1 cut(s) | 500 |
| FaeI | CATG | 5 cut(s) | 260, 299, 626, 665, 920 |
| FaiI | YATR | 7 cut(s) | 142, 154, 258, 297, 624, 663, 918 |
| FaqI | GGGAC | 1 cut(s) | 864 |
| FatI | CATG | 5 cut(s) | 256, 295, 622, 661, 916 |
| Fnu4HI | GCNGC | 1 cut(s) | 921 |
| FokI | GGATG | 3 cut(s) | 499, 566, 578 |
| Fsp4HI | GCNGC | 1 cut(s) | 921 |
| FspBI | CTAG | 1 cut(s) | 104 |
| GlaI | GCGC | 1 cut(s) | 227 |
| GluI | GCNGC | 1 cut(s) | 921 |
| HaeII | RGCGCY | 1 cut(s) | 229 |
| HaeIII | GGCC | 2 cut(s) | 764, 854 |
| HapII | CCGG | 8 cut(s) | 223, 240, 335, 370, 379, 439, 503, 646 |
| HhaI | GCGC | 1 cut(s) | 228 |
| Hin1II | CATG | 5 cut(s) | 260, 299, 626, 665, 920 |
| Hin6I | GCGC | 1 cut(s) | 226 |
| HinP1I | GCGC | 1 cut(s) | 226 |
| HincII | GTYRAC | 1 cut(s) | 184 |
| HindII | GTYRAC | 1 cut(s) | 184 |
| HinfI | GANTC | 5 cut(s) | 584, 601, 702, 776, 932 |
| HpaII | CCGG | 8 cut(s) | 223, 240, 335, 370, 379, 439, 503, 646 |
| HphI | GGTGA | 2 cut(s) | 833, 927 |
| Hpy166II | GTNNAC | 7 cut(s) | 184, 202, 412, 436, 451, 549, 620 |
| Hpy188I | TCNGA | 4 cut(s) | 459, 486, 589, 1008 |
| Hpy188III | TCNNGA | 4 cut(s) | 208, 503, 683, 800 |
| Hpy8I | GTNNAC | 7 cut(s) | 184, 202, 412, 436, 451, 549, 620 |
| Hpy99I | CGWCG | 1 cut(s) | 182 |
| HpyAV | CCTTC | 4 cut(s) | 511, 556, 571, 794 |
| HpyCH4III | ACNGT | 6 cut(s) | 199, 455, 467, 511, 633, 946 |
| HpyCH4IV | ACGT | 2 cut(s) | 180, 330 |
| HpyCH4V | TGCA | 4 cut(s) | 202, 865, 920, 953 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 267, 340 |
| HpyF3I | CTNAG | 2 cut(s) | 458, 492 |
| HpySE526I | ACGT | 2 cut(s) | 180, 330 |
| Hsp92II | CATG | 5 cut(s) | 260, 299, 626, 665, 920 |
| HspAI | GCGC | 1 cut(s) | 226 |
| KflI | GGGWCCC | 1 cut(s) | 878 |
| Kpn2I | TCCGGA | 1 cut(s) | 502 |
| Kzo9I | GATC | 2 cut(s) | 100, 625 |
| LmnI | GCTCC | 2 cut(s) | 381, 742 |
| Lsp1109I | GCAGC | 1 cut(s) | 932 |
| LweI | GCATC | 1 cut(s) | 59 |
| MaeI | CTAG | 1 cut(s) | 104 |
| MaeII | ACGT | 2 cut(s) | 180, 330 |
| MaeIII | GTNAC | 1 cut(s) | 821 |
| MalI | GATC | 2 cut(s) | 102, 627 |
| MboI | GATC | 2 cut(s) | 100, 625 |
| MboII | GAAGA | 5 cut(s) | 22, 25, 103, 433, 829 |
| MflI | RGATCY | 1 cut(s) | 100 |
| MhlI | GDGCHC | 1 cut(s) | 204 |
| MlsI | TGGCCA | 1 cut(s) | 764 |
| MluCI | AATT | 5 cut(s) | 57, 271, 813, 890, 1002 |
| MluNI | TGGCCA | 1 cut(s) | 764 |
| MlyI | GAGTC | 1 cut(s) | 696 |
| MmeI | TCCRAC | 1 cut(s) | 354 |
| MnlI | CCTC | 8 cut(s) | 50, 80, 95, 260, 379, 704, 997, 1006 |
| Mox20I | TGGCCA | 1 cut(s) | 764 |
| Mph1103I | ATGCAT | 1 cut(s) | 955 |
| MroI | TCCGGA | 1 cut(s) | 502 |
| MroXI | GAANNNNTTC | 1 cut(s) | 26 |
| MscI | TGGCCA | 1 cut(s) | 764 |
| MseI | TTAA | 1 cut(s) | 638 |
| MslI | CAYNNNNRTG | 1 cut(s) | 300 |
| Msp20I | TGGCCA | 1 cut(s) | 764 |
| MspI | CCGG | 8 cut(s) | 223, 240, 335, 370, 379, 439, 503, 646 |
| MspR9I | CCNGG | 2 cut(s) | 240, 313 |
| Mva1269I | GAATGC | 1 cut(s) | 28 |
| MvaI | CCWGG | 1 cut(s) | 313 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 267, 340 |
| NciI | CCSGG | 1 cut(s) | 240 |
| NdeII | GATC | 2 cut(s) | 100, 625 |
| NlaIII | CATG | 5 cut(s) | 260, 299, 626, 665, 920 |
| NlaIV | GGNNCC | 4 cut(s) | 304, 681, 879, 880 |
| NmeAIII | GCCGAG | 1 cut(s) | 675 |
| NmuCI | GTSAC | 1 cut(s) | 821 |
| NsiI | ATGCAT | 1 cut(s) | 955 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 183 |
| PctI | GAATGC | 1 cut(s) | 28 |
| PdmI | GAANNNNTTC | 1 cut(s) | 26 |
| PfeI | GAWTC | 4 cut(s) | 584, 601, 776, 932 |
| PflFI | GACNNNGTC | 2 cut(s) | 689, 944 |
| PfoI | TCCNGGA | 1 cut(s) | 311 |
| PinAI | ACCGGT | 3 cut(s) | 369, 438, 645 |
| PkrI | GCNGC | 1 cut(s) | 922 |
| PleI | GAGTC | 1 cut(s) | 696 |
| PpsI | GAGTC | 1 cut(s) | 696 |
| Ppu21I | YACGTR | 1 cut(s) | 331 |
| PpuMI | RGGWCCY | 1 cut(s) | 878 |
| PshAI | GACNNNNGTC | 1 cut(s) | 912 |
| Psp5II | RGGWCCY | 1 cut(s) | 878 |
| Psp6I | CCWGG | 1 cut(s) | 311 |
| PspGI | CCWGG | 1 cut(s) | 311 |
| PspN4I | GGNNCC | 4 cut(s) | 304, 681, 879, 880 |
| PspPI | GGNCC | 4 cut(s) | 376, 436, 853, 878 |
| PspPPI | RGGWCCY | 1 cut(s) | 878 |
| PsuI | RGATCY | 1 cut(s) | 100 |
| PsyI | GACNNNGTC | 2 cut(s) | 689, 944 |
| RsaI | GTAC | 3 cut(s) | 172, 329, 656 |
| RsaNI | GTAC | 3 cut(s) | 171, 328, 655 |
| RseI | CAYNNNNRTG | 1 cut(s) | 300 |
| SaqAI | TTAA | 1 cut(s) | 638 |
| SatI | GCNGC | 1 cut(s) | 921 |
| Sau3AI | GATC | 2 cut(s) | 100, 625 |
| Sau96I | GGNCC | 4 cut(s) | 376, 436, 853, 878 |
| SchI | GAGTC | 1 cut(s) | 696 |
| ScrFI | CCNGG | 2 cut(s) | 240, 313 |
| SduI | GDGCHC | 1 cut(s) | 204 |
| SfaNI | GCATC | 1 cut(s) | 59 |
| SgrAI | CRCCGGYG | 1 cut(s) | 222 |
| SinI | GGWCC | 3 cut(s) | 376, 436, 878 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 300 |
| Sse9I | AATT | 5 cut(s) | 57, 271, 813, 890, 1002 |
| SsiI | CCGC | 1 cut(s) | 48 |
| SspI | AATATT | 1 cut(s) | 994 |
| SspMI | CTAG | 1 cut(s) | 104 |
| StyD4I | CCNGG | 2 cut(s) | 238, 311 |
| TaaI | ACNGT | 6 cut(s) | 199, 455, 467, 511, 633, 946 |
| TaiI | ACGT | 2 cut(s) | 183, 333 |
| TaqI | TCGA | 2 cut(s) | 177, 231 |
| TasI | AATT | 5 cut(s) | 57, 271, 813, 890, 1002 |
| TatI | WGTACW | 2 cut(s) | 170, 654 |
| TfiI | GAWTC | 4 cut(s) | 584, 601, 776, 932 |
| Tru1I | TTAA | 1 cut(s) | 638 |
| Tru9I | TTAA | 1 cut(s) | 638 |
| TseFI | GTSAC | 1 cut(s) | 821 |
| TseI | GCWGC | 1 cut(s) | 920 |
| Tsp45I | GTSAC | 1 cut(s) | 821 |
| TspDTI | ATGAA | 4 cut(s) | 245, 570, 656, 735 |
| TspGWI | ACGGA | 2 cut(s) | 279, 566 |
| Tth111I | GACNNNGTC | 2 cut(s) | 689, 944 |
| VneI | GTGCAC | 1 cut(s) | 200 |
| VpaK11BI | GGWCC | 3 cut(s) | 376, 436, 878 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 310, 797 |
| XapI | RAATTY | 1 cut(s) | 1002 |
| XmnI | GAANNNNTTC | 1 cut(s) | 26 |
| XspI | CTAG | 1 cut(s) | 104 |
| Zsp2I | ATGCAT | 1 cut(s) | 955 |