Rroxscaffold_2G00100520

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001654
Physical Location & Seq
Forward (+)
22375635 .. 22377331
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100520.1

Sequence Viewer

Length: 984 bp
ATGGCTTACCAAAAGATTTGGATTGTCATCTCCCTCTACCTTTCAGCCATATCAACCATTTTTCACCACCTCAAATCCTTCTTGTTCAAAACTAACTGGGATTCAATCTTTTTCAGAATCCCAGATACACTTGTATCCCTATACTTCAGACTATGCGGTCTATCTCCTTGCACCGTTGACATTGACGACCAAACCACAGTCCACTTCTGGACCTCCAACCACCGCCGGTTCGATAAACCAAGCCTGGTTCTGGTTCACGGCTATGGCGGCAACTCCCTGTGGCAGTTCGTCCGTCAAGTGCATCCCCTGTCCAGGAAGTTCAACGTGTACGTGCCGGATTTGCTGTTCTTCGGGAAGTCTTACACGAACCGGTCGGACCGGAGCGAGTCTTTTCAAGCCAAGTGCGTTATGGAGGGGCTGAAGAGGCTGGGCGTGGACCGGTACTCGGTGTACGGTATTAGCTACGGCGGCTTCGTGGCGTACCGCATGGCAGAGATGTGTCCGGAGACAGTGGAGAAGGTTGTGATCGTGAGTAGTGGGGTGGTGTGGAATGATCAGCAGAAGGAGGAGTTGTTGAGGAAGAATTCAAACGCTTTGGAGATTCTTGTAGCGAATAATCCACATGATCTACGGTTGTTAGTGAATCGATCGATTTACAAGTATGATGTTTTCAAGTGGGTTCCTGACTATGTCCTTCAAAAGCTCATTGAGGCGAACAAGGAAAATAGAAAGGAAAAATTAGAGCTATTGGAACACTTGGTGGCAAAGAAAGCAGATCCAAGCCTTGCTATCCTAAGTCAGGAAACGCTAATAATTTGGGGTGATCAAGATAATGTCTTCCCACTTTACATGGCCTACCAGTTGCAAAGGCACTTGGGCCCCAAATCAAAACTAGAGATTATCAAGGACACAGGGCATGCAGCAAATTTTGATTCACCGGACACAATCAATGGATTGATAAAGTCATTTGTTTTGGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

327

Amino Acids

37.78

Weight (kDa)

8.83

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 80 - 313 5.3e-24 alpha/beta hydrolase fold
Hydrolase_4 PF12146 81 - 310 8.6e-06 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 82 - 316 5.1e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 156
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 5 cut(s) 156, 223, 267, 468, 484
AclWI GGATC 1 cut(s) 770
AcsI RAATTY 2 cut(s) 583, 925
AcuI CTGAAG 2 cut(s) 130, 440
AdeI CACNNNGTG 1 cut(s) 760
AfaI GTAC 4 cut(s) 329, 443, 452, 482
AfiI CCNNNNNNNGG 4 cut(s) 250, 312, 445, 799
AflIII ACRYGT 1 cut(s) 324
AgeI ACCGGT 2 cut(s) 369, 438
AgsI TTSAA 7 cut(s) 88, 105, 322, 395, 588, 673, 698
AjnI CCWGG 2 cut(s) 243, 311
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 3 cut(s) 462, 703, 745
AluI AGCT 3 cut(s) 462, 703, 745
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 770
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 852, 877
ApaI GGGCCC 1 cut(s) 881
ApeKI GCWGC 1 cut(s) 920
ApoI RAATTY 2 cut(s) 583, 925
AsiGI ACCGGT 2 cut(s) 369, 438
AspS9I GGNCC 5 cut(s) 210, 376, 436, 877, 878
AsuHPI GGTGA 3 cut(s) 56, 833, 927
AvaII GGWCC 3 cut(s) 210, 376, 436
BaeGI GKGCMC 1 cut(s) 881
BaeI ACNNNNGTAYC 4 cut(s) 117, 117, 150, 150
BanII GRGCYC 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 829
BbvI GCAGC 1 cut(s) 932
BceAI ACGGC 2 cut(s) 274, 481
BciT130I CCWGG 2 cut(s) 245, 313
BciVI GTATCC 1 cut(s) 145
BclI TGATCA 2 cut(s) 553, 823
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 893
BfuI GTATCC 1 cut(s) 145
BisI GCNGC 3 cut(s) 268, 469, 921
BlsI GCNGC 3 cut(s) 269, 470, 922
Bme1390I CCNGG 2 cut(s) 245, 313
Bme18I GGWCC 3 cut(s) 210, 376, 436
BmgT120I GGNCC 5 cut(s) 210, 376, 436, 877, 878
BmiI GGNNCC 3 cut(s) 681, 879, 880
BmrFI CCNGG 2 cut(s) 245, 313
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 829
Bsa29I ATCGAT 2 cut(s) 646, 650
BsaAI YACGTR 1 cut(s) 331
BsaBI GATNNNNATC 1 cut(s) 26
BsaWI WCCGGW 5 cut(s) 369, 378, 438, 502, 937
BsaXI ACNNNNNCTCC 2 cut(s) 557, 587
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 312, 445, 799
Bse118I RCCGGY 3 cut(s) 225, 369, 438
Bse1I ACTGG 2 cut(s) 101, 859
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 2 cut(s) 245, 313
BseCI ATCGAT 2 cut(s) 646, 650
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 4 cut(s) 250, 312, 445, 799
BseNI ACTGG 2 cut(s) 101, 859
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 881
BseXI GCAGC 1 cut(s) 932
BseYI CCCAGC 1 cut(s) 427
Bsh1285I CGRYCG 2 cut(s) 374, 650
BshFI GGCC 2 cut(s) 854, 879
BshTI ACCGGT 2 cut(s) 369, 438
BshVI ATCGAT 2 cut(s) 646, 650
BsiEI CGRYCG 2 cut(s) 374, 650
BsiSI CCGG 7 cut(s) 226, 335, 370, 379, 439, 503, 938
BslI CCNNNNNNNGG 4 cut(s) 250, 312, 445, 799
BsmAI GTCTC 1 cut(s) 500
BsnI GGCC 2 cut(s) 854, 879
Bsp120I GGGCCC 1 cut(s) 877
Bsp1286I GDGCHC 1 cut(s) 881
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 6 cut(s) 525, 553, 625, 647, 775, 823
BspACI CCGC 5 cut(s) 156, 223, 267, 468, 484
BspANI GGCC 2 cut(s) 854, 879
BspDI ATCGAT 2 cut(s) 646, 650
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 681, 879, 880
BspPI GGATC 1 cut(s) 770
BsrFI RCCGGY 3 cut(s) 225, 369, 438
BsrI ACTGG 2 cut(s) 101, 859
BssAI RCCGGY 3 cut(s) 225, 369, 438
BssMI GATC 6 cut(s) 525, 553, 625, 647, 775, 823
Bst2UI CCWGG 2 cut(s) 245, 313
Bst4CI ACNGT 5 cut(s) 175, 199, 455, 511, 633
Bst6I CTCTTC 1 cut(s) 416
BstBAI YACGTR 1 cut(s) 331
BstC8I GCNNGC 1 cut(s) 918
BstDEI CTNAG 1 cut(s) 794
BstENI CCTNNNNNAGG 1 cut(s) 797
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 6 cut(s) 528, 556, 628, 650, 778, 826
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 6 cut(s) 525, 553, 625, 647, 775, 823
BstMCI CGRYCG 2 cut(s) 374, 650
BstMWI GCNNNNNNNGC 5 cut(s) 267, 340, 424, 468, 770
BstNI CCWGG 2 cut(s) 245, 313
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 2 cut(s) 243, 311
BstSLI GKGCMC 1 cut(s) 881
BstV1I GCAGC 1 cut(s) 932
BstV2I GAAGAC 1 cut(s) 829
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
Bsu15I ATCGAT 2 cut(s) 646, 650
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 2 cut(s) 854, 879
BsuTUI ATCGAT 2 cut(s) 646, 650
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 918
Cfr10I RCCGGY 3 cut(s) 225, 369, 438
Cfr13I GGNCC 5 cut(s) 210, 376, 436, 877, 878
ClaI ATCGAT 2 cut(s) 646, 650
CpoI CGGWCCG 1 cut(s) 376
Csp6I GTAC 4 cut(s) 328, 442, 451, 481
CspAI ACCGGT 2 cut(s) 369, 438
CspI CGGWCCG 1 cut(s) 376
CviAII CATG 4 cut(s) 487, 623, 850, 917
CviQI GTAC 4 cut(s) 328, 442, 451, 481
DdeI CTNAG 1 cut(s) 794
DpnI GATC 6 cut(s) 527, 555, 627, 649, 777, 825
DpnII GATC 6 cut(s) 525, 553, 625, 647, 775, 823
DraIII CACNNNGTG 1 cut(s) 760
DrdI GACNNNNNNGTC 1 cut(s) 156
DseDI GACNNNNNNGTC 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 881
Eco47I GGWCC 3 cut(s) 210, 376, 436
Eco57I CTGAAG 2 cut(s) 130, 440
EcoNI CCTNNNNNAGG 1 cut(s) 797
EcoO109I RGGNCCY 1 cut(s) 878
EcoRI GAATTC 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 243, 311
EcoT38I GRGCYC 1 cut(s) 881
FaeI CATG 4 cut(s) 490, 626, 853, 920
FatI CATG 4 cut(s) 486, 622, 849, 916
FbaI TGATCA 2 cut(s) 553, 823
Fnu4HI GCNGC 3 cut(s) 268, 469, 921
FokI GGATG 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 3 cut(s) 268, 469, 921
FspBI CTAG 1 cut(s) 893
GluI GCNGC 3 cut(s) 268, 469, 921
GsaI CCCAGC 1 cut(s) 431
HaeIII GGCC 2 cut(s) 854, 879
HapII CCGG 7 cut(s) 226, 335, 370, 379, 439, 503, 938
Hin1II CATG 4 cut(s) 490, 626, 853, 920
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 6 cut(s) 101, 117, 386, 601, 643, 932
HpaII CCGG 7 cut(s) 226, 335, 370, 379, 439, 503, 938
HphI GGTGA 3 cut(s) 56, 833, 927
Hpy166II GTNNAC 6 cut(s) 178, 202, 256, 328, 436, 451
Hpy188I TCNGA 3 cut(s) 116, 149, 376
Hpy188III TCNNGA 7 cut(s) 208, 352, 503, 529, 683, 800, 827
Hpy8I GTNNAC 6 cut(s) 178, 202, 256, 328, 436, 451
HpyAV CCTTC 4 cut(s) 88, 511, 556, 704
HpyCH4III ACNGT 5 cut(s) 175, 199, 455, 511, 633
HpyCH4IV ACGT 2 cut(s) 324, 330
HpyCH4V TGCA 4 cut(s) 171, 301, 865, 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 267, 340, 424, 468, 770
HpyF3I CTNAG 1 cut(s) 794
HpySE526I ACGT 2 cut(s) 324, 330
Hsp92II CATG 4 cut(s) 490, 626, 853, 920
Kpn2I TCCGGA 1 cut(s) 502
Ksp22I TGATCA 2 cut(s) 553, 823
Kzo9I GATC 6 cut(s) 525, 553, 625, 647, 775, 823
LmnI GCTCC 1 cut(s) 381
Lsp1109I GCAGC 1 cut(s) 932
LweI GCATC 1 cut(s) 310
MaeI CTAG 1 cut(s) 893
MaeII ACGT 2 cut(s) 324, 330
MalI GATC 6 cut(s) 527, 555, 627, 649, 777, 825
MboI GATC 6 cut(s) 525, 553, 625, 647, 775, 823
MboII GAAGA 4 cut(s) 340, 433, 592, 829
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 881
MluCI AATT 4 cut(s) 583, 737, 813, 925
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 240, 354
MnlI CCTC 8 cut(s) 44, 80, 223, 406, 417, 559, 570, 703
MroI TCCGGA 1 cut(s) 502
MseI TTAA 1 cut(s) 982
MslI CAYNNNNRTG 1 cut(s) 261
MspI CCGG 7 cut(s) 226, 335, 370, 379, 439, 503, 938
MspR9I CCNGG 2 cut(s) 245, 313
MvaI CCWGG 2 cut(s) 245, 313
MwoI GCNNNNNNNGC 5 cut(s) 267, 340, 424, 468, 770
NdeII GATC 6 cut(s) 525, 553, 625, 647, 775, 823
NlaIII CATG 4 cut(s) 490, 626, 853, 920
NlaIV GGNNCC 3 cut(s) 681, 879, 880
NspI RCATGY 1 cut(s) 920
PaeI GCATGC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PfeI GAWTC 5 cut(s) 101, 117, 601, 643, 932
PflFI GACNNNGTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 311
PinAI ACCGGT 2 cut(s) 369, 438
PkrI GCNGC 3 cut(s) 269, 470, 922
Ple19I CGATCG 1 cut(s) 650
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 243, 311
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 2 cut(s) 243, 311
PspN4I GGNNCC 3 cut(s) 681, 879, 880
PspOMI GGGCCC 1 cut(s) 877
PspPI GGNCC 5 cut(s) 210, 376, 436, 877, 878
PsuI RGATCY 1 cut(s) 775
PsyI GACNNNGTC 1 cut(s) 689
PvuI CGATCG 1 cut(s) 650
RsaI GTAC 4 cut(s) 329, 443, 452, 482
RsaNI GTAC 4 cut(s) 328, 442, 451, 481
RseI CAYNNNNRTG 1 cut(s) 261
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SaqAI TTAA 1 cut(s) 982
SatI GCNGC 3 cut(s) 268, 469, 921
Sau3AI GATC 6 cut(s) 525, 553, 625, 647, 775, 823
Sau96I GGNCC 5 cut(s) 210, 376, 436, 877, 878
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 2 cut(s) 245, 313
SduI GDGCHC 1 cut(s) 881
SetI ASST 9 cut(s) 42, 72, 215, 327, 333, 464, 522, 705, 747
SfaNI GCATC 1 cut(s) 310
SinI GGWCC 3 cut(s) 210, 376, 436
SmiMI CAYNNNNRTG 1 cut(s) 261
SphI GCATGC 1 cut(s) 920
Sse9I AATT 4 cut(s) 583, 737, 813, 925
SsiI CCGC 5 cut(s) 156, 223, 267, 468, 484
SspMI CTAG 1 cut(s) 893
StyD4I CCNGG 2 cut(s) 243, 311
TaaI ACNGT 5 cut(s) 175, 199, 455, 511, 633
TaiI ACGT 2 cut(s) 327, 333
TaqI TCGA 3 cut(s) 231, 646, 650
TasI AATT 4 cut(s) 583, 737, 813, 925
TauI GCSGC 2 cut(s) 270, 471
TfiI GAWTC 5 cut(s) 101, 117, 601, 643, 932
Tru1I TTAA 1 cut(s) 982
Tru9I TTAA 1 cut(s) 982
TscAI CASTG 1 cut(s) 516
TseI GCWGC 1 cut(s) 920
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 516
Tth111I GACNNNGTC 1 cut(s) 689
VpaK11BI GGWCC 3 cut(s) 210, 376, 436
XagI CCTNNNNNAGG 1 cut(s) 797
XapI RAATTY 2 cut(s) 583, 925
XceI RCATGY 1 cut(s) 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.