Rw2G036530

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001654
Physical Location & Seq
Reverse (-)
60122473 .. 60124355
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036530.1

Sequence Viewer

Length: 972 bp
ATGGCGTACACGAAGTTCTATCATGTCCTCTCCTTCTACCTCGCAACCCTAATAGCCATATACGAATTCCTTCTTTCAGTCTTGCCTTATATTTTGAATCCTGCTTCCATCATAATCGCTTTTGTAGATGCATTTTTAGGAGTATACTTCAGATTGTGTTTAGATGAACAAACCACCATGCATTTTTGGGCTTCCAATCCTAGAAGATCTGACAAGCCTGATCTACTGATGCTTCACGGGTATGGTGGCAATTCCAAGTGGCAATTCATGCACCAAGTCAGCTCACTCTCCCGGACTTTTAATTTGTACGTGCCCGATTTACTCTTCTTTGGCAAGTCATACACAAAAATGTCCGACAGGACGGAGGTGTTCCAAGCAAAATGTGTAGCGGAAGGGTTGAGGAGACTAGGCGTTGACAGGTTTGGTGTGTACTCTATAAGCTATGGAGGGTTTGTGGCATATTGGCTGGCGGAGATGTATCCGCAGGCGGTGGAGAGGCTTGTGATTGTGAGCTGTGGGATAGGGATGACAGAGGAGCAGAAAAAGGATCAGCTCAACAAGATTGGACGCAATGCATTGAAGCTCCTTGTACCTGAAAGTGCTCATGATCTAAGGGGCTTGGTGAATCTTTCGGTGCACAAACCCAGTCCTGTTAGGTGGCTACCCGATGTTTTCATCCGCGGGTTTATCCATGTGACATATGAGAGAAATAGGAAGCAAAAACTAGAGATGGCAGAGCACTTGCTAAGGGAAAAAGCAGACCTAAACCTTCCCATTCTGACTCAGGAAACACTAATAATCTGGGGTGACAAGGATGCTGTCTTCCCAGTGAACTTGGCCTATGAGCTGCAAAGGTATTTGGGACCAAAATCAAAGGTAGAGATAATCAAGGACACAGGTCATGCAGTGAATATGGATGCACCCATCTCCCTGAATGCACTGATAACATCTTTTGTTTTGGGTTACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

323

Amino Acids

37.04

Weight (kDa)

8.75

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 75 - 172 1.6e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 75 - 308 9.8e-11 Alpha/beta hydrolase family
Hydrolase_4 PF12146 140 - 305 3.7e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 144
AccII CGCG 1 cut(s) 681
AciI CCGC 6 cut(s) 389, 470, 482, 488, 679, 681
AclWI GGATC 1 cut(s) 555
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 133
AfaI GTAC 4 cut(s) 8, 308, 431, 591
AgsI TTSAA 2 cut(s) 97, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 282, 441, 513, 553, 583, 847
AluI AGCT 6 cut(s) 282, 441, 513, 553, 583, 847
Alw21I GWGCWC 3 cut(s) 604, 639, 741
Alw26I GTCTC 1 cut(s) 397
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 1 cut(s) 555
AoxI GGCC 1 cut(s) 837
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 1 cut(s) 847
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
Asp700I GAANNNNTTC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 863
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 2 cut(s) 634, 818
AvaII GGWCC 1 cut(s) 863
BaeGI GKGCMC 2 cut(s) 315, 639
BbsI GAAGAC 1 cut(s) 814
Bbv12I GWGCWC 3 cut(s) 604, 639, 741
BbvI GCAGC 1 cut(s) 834
BccI CCATC 3 cut(s) 116, 724, 932
BciVI GTATCC 1 cut(s) 489
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 3 cut(s) 201, 407, 725
BfuI GTATCC 1 cut(s) 489
BglII AGATCT 1 cut(s) 206
BisI GCNGC 1 cut(s) 848
BlsI GCNGC 1 cut(s) 849
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 1 cut(s) 863
BmiI GGNNCC 1 cut(s) 864
BmrFI CCNGG 1 cut(s) 292
BmrI ACTGGG 2 cut(s) 639, 821
BmsI GCATC 4 cut(s) 118, 219, 805, 907
BmuI ACTGGG 2 cut(s) 639, 821
BoxI GACNNNNGTC 1 cut(s) 897
BpiI GAAGAC 1 cut(s) 814
Bpu10I CCTNAGC 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 679
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bse1I ACTGG 2 cut(s) 645, 827
Bse3DI GCAATG 1 cut(s) 577
BseDI CCNNGG 1 cut(s) 679
BseGI GGATG 4 cut(s) 531, 675, 820, 922
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 1 cut(s) 797
BseNI ACTGG 2 cut(s) 645, 827
BseRI GAGGAG 2 cut(s) 415, 548
BseSI GKGCMC 2 cut(s) 315, 639
BseXI GCAGC 1 cut(s) 834
Bsh1236I CGCG 1 cut(s) 681
BshFI GGCC 1 cut(s) 839
BsiHKAI GWGCWC 3 cut(s) 604, 639, 741
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 876
BsmAI GTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 876
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 1 cut(s) 839
Bsp1286I GDGCHC 4 cut(s) 315, 604, 639, 741
Bsp143I GATC 4 cut(s) 206, 220, 547, 607
BspACI CCGC 6 cut(s) 389, 470, 482, 488, 679, 681
BspANI GGCC 1 cut(s) 839
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 681
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 864
BspPI GGATC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 577
BsrI ACTGG 2 cut(s) 645, 827
BssECI CCNNGG 1 cut(s) 679
BssMI GATC 4 cut(s) 206, 220, 547, 607
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 468, 486
BstDEI CTNAG 3 cut(s) 611, 746, 783
BstDSI CCRYGG 1 cut(s) 679
BstF5I GGATG 4 cut(s) 531, 675, 820, 922
BstFNI CGCG 1 cut(s) 681
BstKTI GATC 4 cut(s) 209, 223, 550, 610
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 4 cut(s) 206, 220, 547, 607
BstMWI GCNNNNNNNGC 1 cut(s) 268
BstPAI GACNNNNGTC 1 cut(s) 897
BstSCI CCNGG 1 cut(s) 290
BstSLI GKGCMC 2 cut(s) 315, 639
BstUI CGCG 1 cut(s) 681
BstV1I GCAGC 1 cut(s) 834
BstV2I GAAGAC 1 cut(s) 814
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BstZ17I GTATAC 1 cut(s) 145
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 1 cut(s) 839
BtgI CCRYGG 1 cut(s) 679
BtsCI GGATG 4 cut(s) 531, 675, 820, 922
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 3 cut(s) 834, 912, 938
Cac8I GCNNGC 2 cut(s) 468, 486
CciI TCATGA 1 cut(s) 604
Cfr13I GGNCC 1 cut(s) 863
Cfr42I CCGCGG 1 cut(s) 682
CseI GACGC 1 cut(s) 576
Csp6I GTAC 4 cut(s) 7, 307, 430, 590
CviAII CATG 6 cut(s) 23, 178, 268, 605, 692, 902
CviQI GTAC 4 cut(s) 7, 307, 430, 590
DdeI CTNAG 3 cut(s) 611, 746, 783
DpnI GATC 4 cut(s) 208, 222, 549, 609
DpnII GATC 4 cut(s) 206, 220, 547, 607
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
EciI GGCGGA 1 cut(s) 485
Eco47I GGWCC 1 cut(s) 863
Eco57I CTGAAG 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 65
EcoT22I ATGCAT 3 cut(s) 133, 183, 577
FaeI CATG 6 cut(s) 26, 181, 271, 608, 695, 905
FaqI GGGAC 1 cut(s) 876
FatI CATG 6 cut(s) 22, 177, 267, 604, 691, 901
FauI CCCGC 1 cut(s) 674
FauNDI CATATG 1 cut(s) 700
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 848
FokI GGATG 4 cut(s) 538, 662, 827, 929
Fsp4HI GCNGC 1 cut(s) 848
FspBI CTAG 3 cut(s) 201, 407, 725
GluI GCNGC 1 cut(s) 848
HaeIII GGCC 1 cut(s) 839
HapII CCGG 1 cut(s) 292
HgaI GACGC 1 cut(s) 576
Hin1II CATG 6 cut(s) 26, 181, 271, 608, 695, 905
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 97, 625, 781
HpaII CCGG 1 cut(s) 292
HphI GGTGA 2 cut(s) 634, 818
Hpy166II GTNNAC 6 cut(s) 9, 145, 415, 430, 637, 832
Hpy188I TCNGA 4 cut(s) 152, 211, 355, 780
Hpy188III TCNNGA 2 cut(s) 605, 785
Hpy8I GTNNAC 6 cut(s) 9, 145, 415, 430, 637, 832
HpyAV CCTTC 4 cut(s) 43, 80, 386, 779
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 9 cut(s) 131, 181, 271, 575, 637, 850, 905, 920, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 268
HpyF3I CTNAG 3 cut(s) 611, 746, 783
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 6 cut(s) 26, 181, 271, 608, 695, 905
KspI CCGCGG 1 cut(s) 682
Kzo9I GATC 4 cut(s) 206, 220, 547, 607
LmnI GCTCC 2 cut(s) 535, 588
Lsp1109I GCAGC 1 cut(s) 834
LweI GCATC 4 cut(s) 118, 219, 805, 907
MaeI CTAG 3 cut(s) 201, 407, 725
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 3 cut(s) 694, 806, 962
MalI GATC 4 cut(s) 208, 222, 549, 609
MboI GATC 4 cut(s) 206, 220, 547, 607
MboII GAAGA 3 cut(s) 216, 316, 814
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 4 cut(s) 315, 604, 639, 741
MluCI AATT 4 cut(s) 65, 250, 263, 301
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 7 cut(s) 38, 50, 358, 393, 440, 489, 526
Mph1103I ATGCAT 3 cut(s) 133, 183, 577
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 240, 347
MspA1I CMGCKG 1 cut(s) 681
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 940
MvnI CGCG 1 cut(s) 681
MwoI GCNNNNNNNGC 1 cut(s) 268
NciI CCSGG 1 cut(s) 292
NdeI CATATG 1 cut(s) 700
NdeII GATC 4 cut(s) 206, 220, 547, 607
NlaIII CATG 6 cut(s) 26, 181, 271, 608, 695, 905
NlaIV GGNNCC 1 cut(s) 864
NmuCI GTSAC 2 cut(s) 694, 806
NsiI ATGCAT 3 cut(s) 133, 183, 577
PagI TCATGA 1 cut(s) 604
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 97, 625
PfoI TCCNGGA 1 cut(s) 290
PkrI GCNGC 1 cut(s) 849
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
Ppu21I YACGTR 1 cut(s) 310
PshAI GACNNNNGTC 1 cut(s) 897
PspN4I GGNNCC 1 cut(s) 864
PspPI GGNCC 1 cut(s) 863
PsrI GAACNNNNNNTAC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 4 cut(s) 8, 308, 431, 591
RsaNI GTAC 4 cut(s) 7, 307, 430, 590
RseI CAYNNNNRTG 2 cut(s) 240, 347
SacII CCGCGG 1 cut(s) 682
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 848
Sau3AI GATC 4 cut(s) 206, 220, 547, 607
Sau96I GGNCC 1 cut(s) 863
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 4 cut(s) 315, 604, 639, 741
SfaNI GCATC 4 cut(s) 118, 219, 805, 907
Sfr303I CCGCGG 1 cut(s) 682
SgrBI CCGCGG 1 cut(s) 682
SinI GGWCC 1 cut(s) 863
SmiMI CAYNNNNRTG 2 cut(s) 240, 347
Sse9I AATT 4 cut(s) 65, 250, 263, 301
SsiI CCGC 6 cut(s) 389, 470, 482, 488, 679, 681
SspMI CTAG 3 cut(s) 201, 407, 725
StyD4I CCNGG 1 cut(s) 290
TaiI ACGT 1 cut(s) 312
TasI AATT 4 cut(s) 65, 250, 263, 301
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 2 cut(s) 97, 625
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 834, 912, 945
TseFI GTSAC 2 cut(s) 694, 806
TseI GCWGC 1 cut(s) 847
Tsp45I GTSAC 2 cut(s) 694, 806
TspDTI ATGAA 3 cut(s) 180, 256, 664
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 3 cut(s) 834, 912, 945
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 863
XapI RAATTY 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 3 cut(s) 201, 407, 725
Zsp2I ATGCAT 3 cut(s) 133, 183, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.