pycom09g09980

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001654
Physical Location & Seq
Forward (+)
8073554 .. 8075154
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g09980.3

Sequence Viewer

Length: 1032 bp
ATGGCTTTCCAAAAGTTTTGGAATGCTCTCTTCTTCTACCTCTCAACCGCATCAACAATTTTCCACACTCTCTCATCTTTTTTCCTCAAAACCCACTGGGATCTAGTGTTCTTCAAACTCCCAGATACTCTTGTAGCTTTATATTTCAGGCTATGTGGTCTCTCTCCTTGTACAGTCGACGTTGACGACCAAACCACCATGCACTTCTGGACTGCCAACCACCGGCGGTTTGATAAACCCAGTCTCGTTCTGGTTCACGGCTACGGAGGCAATTCTCTATGGCAGTTTGTGTGTCATGTGGGTGCCTTGTCCAGGAAATTCAACTTGTACGTGCCGGATTTGCTGTTCTTTGGGAAGTCTTACACGAACCGGTTGGACCGGAGCGAGGTATTCCAAGCGAAAAGTGTTGTGGACGGGTTGAAGAGATTGGGTGTGGACCGGTTCTCGGTTTACGGTCTGAGCTACGGTGGGTTTGTGGCGTATCGGATGGCTGAGATGTATCCGGAGACGGTGGAGAAGGTTGTGATTGTGAGTAGTGGGGTAGTGTGGACGGATGAACAGAAGGATGAGCTGTTGAAGGTGGAATCAGATGCTTTGGAGACTCTTTTGCCAAGAAGTCCACATGATCTACGGTTGTTAATGAACCGGTCTTTGTACAAGTATGATGTTTTCAAGTGGGTTCCTGACTTTGTCCTCGGCAGACTCATCAAAGCCAACAATGAACACAGAAAGGAGAGAATGGAGCTACTTGAACACTTGGTGGCCAAGAAACAAGATTCAAACCTTCCTATTCTAACTCAGGTAACAAGAGAAACGCTAATAATTTGGGGCGACAAAGATAAAGTCTTCCCATTGTTCTTGGCCCATCAGTTGCAAAGGCATTTGGGTTCCAAATCAAAATTAGAGATAATCAAGGACACAGGTCATGCAGCAAACTTTGATTCACCGGACACAGTCAATGCATTGATAAAATCATTTGTTTTGGATGGTTATCCCCCCAATATTATTGGAATTCCGATGGAGGTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

344

Amino Acids

39.54

Weight (kDa)

8.56

Isoelectric Point (pI)

26.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 177
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 2 cut(s) 48, 226
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 2 cut(s) 317, 1011
AdeI CACNNNGTG 1 cut(s) 760
AfaI GTAC 3 cut(s) 172, 329, 656
AfiI CCNNNNNNNGG 4 cut(s) 222, 312, 385, 445
AgeI ACCGGT 3 cut(s) 369, 438, 645
AgsI TTSAA 7 cut(s) 115, 322, 421, 577, 673, 752, 780
AjnI CCWGG 1 cut(s) 311
AloI GAACNNNNNNTCC 4 cut(s) 92, 124, 329, 361
AluBI AGCT 4 cut(s) 137, 462, 571, 745
AluI AGCT 4 cut(s) 137, 462, 571, 745
Alw26I GTCTC 4 cut(s) 164, 248, 500, 593
AlwI GGATC 1 cut(s) 108
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 762, 861
ApeKI GCWGC 1 cut(s) 929
ApoI RAATTY 2 cut(s) 317, 1011
ArsI GACNNNNNNTTYG 2 cut(s) 828, 860
AsiGI ACCGGT 3 cut(s) 369, 438, 645
AspS9I GGNCC 3 cut(s) 376, 436, 862
AsuHPI GGTGA 1 cut(s) 936
AvaII GGWCC 2 cut(s) 376, 436
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BalI TGGCCA 1 cut(s) 764
BanI GGYRCC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 838
BbvI GCAGC 1 cut(s) 941
BccI CCATC 4 cut(s) 481, 873, 980, 1012
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 313
BciVI GTATCC 1 cut(s) 510
BcoDI GTCTC 4 cut(s) 164, 248, 500, 593
BfaI CTAG 1 cut(s) 104
BfuI GTATCC 1 cut(s) 510
BisI GCNGC 1 cut(s) 930
BlsI GCNGC 1 cut(s) 931
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 2 cut(s) 376, 436
BmgT120I GGNCC 3 cut(s) 376, 436, 862
BmiI GGNNCC 3 cut(s) 304, 681, 889
BmrFI CCNGG 1 cut(s) 313
BmrI ACTGGG 2 cut(s) 106, 234
BmsI GCATC 2 cut(s) 59, 580
BmuI ACTGGG 2 cut(s) 106, 234
BoxI GACNNNNGTC 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 838
BsaAI YACGTR 1 cut(s) 331
BsaBI GATNNNNATC 1 cut(s) 990
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 694
BsaWI WCCGGW 6 cut(s) 369, 378, 438, 502, 645, 946
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 312, 385, 445
Bse118I RCCGGY 4 cut(s) 222, 369, 438, 645
Bse1I ACTGG 2 cut(s) 101, 240
Bse8I GATNNNNATC 1 cut(s) 990
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 694
BseGI GGATG 4 cut(s) 492, 559, 571, 991
BseJI GATNNNNATC 1 cut(s) 990
BseLI CCNNNNNNNGG 4 cut(s) 222, 312, 385, 445
BseMII CTCAG 3 cut(s) 449, 483, 812
BseNI ACTGG 2 cut(s) 101, 240
BseXI GCAGC 1 cut(s) 941
BshFI GGCC 2 cut(s) 764, 863
BshNI GGYRCC 1 cut(s) 302
BshTI ACCGGT 3 cut(s) 369, 438, 645
BsiSI CCGG 8 cut(s) 223, 335, 370, 379, 439, 503, 646, 947
BslI CCNNNNNNNGG 4 cut(s) 222, 312, 385, 445
BsmAI GTCTC 4 cut(s) 164, 248, 500, 593
BsmBI CGTCTC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 2 cut(s) 764, 863
Bso31I GGTCTC 1 cut(s) 164
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 2 cut(s) 170, 654
Bsp143I GATC 2 cut(s) 100, 625
BspACI CCGC 2 cut(s) 48, 226
BspANI GGCC 2 cut(s) 764, 863
BspCNI CTCAG 3 cut(s) 450, 484, 811
BspEI TCCGGA 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 304, 681, 889
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 164
BsrFI RCCGGY 4 cut(s) 222, 369, 438, 645
BsrGI TGTACA 2 cut(s) 170, 654
BsrI ACTGG 2 cut(s) 101, 240
BssAI RCCGGY 4 cut(s) 222, 369, 438, 645
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 2 cut(s) 100, 625
Bst2UI CCWGG 1 cut(s) 313
Bst4CI ACNGT 6 cut(s) 175, 455, 467, 511, 633, 955
Bst6I CTCTTC 2 cut(s) 35, 416
BstAUI TGTACA 2 cut(s) 170, 654
BstBAI YACGTR 1 cut(s) 331
BstDEI CTNAG 3 cut(s) 458, 492, 798
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 4 cut(s) 492, 559, 571, 991
BstKTI GATC 2 cut(s) 103, 628
BstMAI GTCTC 4 cut(s) 164, 248, 500, 593
BstMBI GATC 2 cut(s) 100, 625
BstMWI GCNNNNNNNGC 2 cut(s) 267, 340
BstNI CCWGG 1 cut(s) 313
BstPAI GACNNNNGTC 1 cut(s) 921
BstSCI CCNGG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 941
BstV2I GAAGAC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 2 cut(s) 764, 863
BtsCI GGATG 4 cut(s) 492, 559, 571, 991
BtsIMutI CAGTG 1 cut(s) 94
Cfr10I RCCGGY 4 cut(s) 222, 369, 438, 645
Cfr13I GGNCC 3 cut(s) 376, 436, 862
Csp6I GTAC 3 cut(s) 171, 328, 655
CspAI ACCGGT 3 cut(s) 369, 438, 645
CviAII CATG 4 cut(s) 199, 296, 623, 926
CviQI GTAC 3 cut(s) 171, 328, 655
DdeI CTNAG 3 cut(s) 458, 492, 798
DpnI GATC 2 cut(s) 102, 627
DpnII GATC 2 cut(s) 100, 625
DraIII CACNNNGTG 1 cut(s) 760
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 2 cut(s) 35, 416
EarI CTCTTC 2 cut(s) 35, 416
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 2 cut(s) 376, 436
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 1011
EcoRII CCWGG 1 cut(s) 311
EcoT22I ATGCAT 1 cut(s) 964
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 4 cut(s) 202, 299, 626, 929
FaiI YATR 8 cut(s) 142, 154, 200, 280, 297, 624, 663, 927
FatI CATG 4 cut(s) 198, 295, 622, 925
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 930
FokI GGATG 4 cut(s) 499, 566, 578, 998
Fsp4HI GCNGC 1 cut(s) 930
FspBI CTAG 1 cut(s) 104
GluI GCNGC 1 cut(s) 930
HaeIII GGCC 2 cut(s) 764, 863
HapII CCGG 8 cut(s) 223, 335, 370, 379, 439, 503, 646, 947
Hin1II CATG 4 cut(s) 202, 299, 626, 929
HincII GTYRAC 2 cut(s) 178, 184
HindII GTYRAC 2 cut(s) 178, 184
HinfI GANTC 5 cut(s) 584, 601, 702, 776, 941
HpaII CCGG 8 cut(s) 223, 335, 370, 379, 439, 503, 646, 947
HphI GGTGA 1 cut(s) 936
Hpy166II GTNNAC 8 cut(s) 178, 184, 256, 412, 436, 451, 549, 620
Hpy188I TCNGA 4 cut(s) 459, 486, 589, 1017
Hpy188III TCNNGA 3 cut(s) 208, 503, 683
Hpy8I GTNNAC 8 cut(s) 178, 184, 256, 412, 436, 451, 549, 620
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 4 cut(s) 511, 556, 571, 794
HpyCH4III ACNGT 6 cut(s) 175, 455, 467, 511, 633, 955
HpyCH4IV ACGT 2 cut(s) 180, 330
HpyCH4V TGCA 4 cut(s) 202, 874, 929, 962
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 340
HpyF3I CTNAG 3 cut(s) 458, 492, 798
HpySE526I ACGT 2 cut(s) 180, 330
Hsp92II CATG 4 cut(s) 202, 299, 626, 929
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 2 cut(s) 100, 625
LmnI GCTCC 2 cut(s) 381, 742
Lsp1109I GCAGC 1 cut(s) 941
LweI GCATC 2 cut(s) 59, 580
MaeI CTAG 1 cut(s) 104
MaeII ACGT 2 cut(s) 180, 330
MaeIII GTNAC 1 cut(s) 802
MalI GATC 2 cut(s) 102, 627
MboI GATC 2 cut(s) 100, 625
MboII GAAGA 5 cut(s) 22, 25, 103, 433, 838
MflI RGATCY 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 6 cut(s) 57, 271, 317, 822, 899, 1011
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 2 cut(s) 595, 696
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 6 cut(s) 50, 95, 260, 379, 704, 1015
Mox20I TGGCCA 1 cut(s) 764
Mph1103I ATGCAT 1 cut(s) 964
MroI TCCGGA 1 cut(s) 502
MscI TGGCCA 1 cut(s) 764
MseI TTAA 1 cut(s) 638
MslI CAYNNNNRTG 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 764
MspI CCGG 8 cut(s) 223, 335, 370, 379, 439, 503, 646, 947
MspR9I CCNGG 1 cut(s) 313
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 1 cut(s) 313
MwoI GCNNNNNNNGC 2 cut(s) 267, 340
NdeII GATC 2 cut(s) 100, 625
NlaIII CATG 4 cut(s) 202, 299, 626, 929
NlaIV GGNNCC 3 cut(s) 304, 681, 889
NmeAIII GCCGAG 1 cut(s) 675
NsiI ATGCAT 1 cut(s) 964
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 28
PfeI GAWTC 3 cut(s) 584, 776, 941
PflFI GACNNNGTC 2 cut(s) 689, 953
PfoI TCCNGGA 1 cut(s) 311
PinAI ACCGGT 3 cut(s) 369, 438, 645
PkrI GCNGC 1 cut(s) 931
PleI GAGTC 2 cut(s) 595, 696
PpsI GAGTC 2 cut(s) 595, 696
Ppu21I YACGTR 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 311
PspGI CCWGG 1 cut(s) 311
PspN4I GGNNCC 3 cut(s) 304, 681, 889
PspPI GGNCC 3 cut(s) 376, 436, 862
PsuI RGATCY 1 cut(s) 100
PsyI GACNNNGTC 2 cut(s) 689, 953
RsaI GTAC 3 cut(s) 172, 329, 656
RsaNI GTAC 3 cut(s) 171, 328, 655
RseI CAYNNNNRTG 1 cut(s) 300
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 1 cut(s) 930
Sau3AI GATC 2 cut(s) 100, 625
Sau96I GGNCC 3 cut(s) 376, 436, 862
SchI GAGTC 2 cut(s) 595, 696
ScrFI CCNGG 1 cut(s) 313
SfaNI GCATC 2 cut(s) 59, 580
SgrAI CRCCGGYG 1 cut(s) 222
SinI GGWCC 2 cut(s) 376, 436
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 6 cut(s) 57, 271, 317, 822, 899, 1011
SsiI CCGC 2 cut(s) 48, 226
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 311
TaaI ACNGT 6 cut(s) 175, 455, 467, 511, 633, 955
TaiI ACGT 2 cut(s) 183, 333
TaqI TCGA 1 cut(s) 177
TasI AATT 6 cut(s) 57, 271, 317, 822, 899, 1011
TatI WGTACW 2 cut(s) 170, 654
TfiI GAWTC 3 cut(s) 584, 776, 941
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 1 cut(s) 101
TseI GCWGC 1 cut(s) 929
TspDTI ATGAA 3 cut(s) 570, 656, 735
TspGWI ACGGA 2 cut(s) 279, 566
TspRI CASTG 1 cut(s) 101
Tth111I GACNNNGTC 2 cut(s) 689, 953
VpaK11BI GGWCC 2 cut(s) 376, 436
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 2 cut(s) 317, 1011
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 104
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.