Rorug02G0392500

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001654
Physical Location & Seq
Forward (+)
50287839 .. 50289392
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0392500.1

Sequence Viewer

Length: 1077 bp
ATGGTTTTGATTTCAGGTCTTCCTGATGAAGTAGTCTACGATTGTCTTATTCGTGTCAAGTATGATCAGTTTCCGACTATAACATCTGTTTCTAAAGGTTGGAAATCAGAGGTTGAGCTACCAGAGTTTCATCGCTTCAGAAAGAATGGTGGTTATGGCCAAAAGCTCATTGTGATGGTTCAAGCACGTGTTGCTCCCAACCAAGGTGGTGGTGCTGGTGTCTTCAAGTGCCTGAAAAAACCGCTGTACCGGTTCACCGTCTGTGAGCCGGATTCGGGTGATTGGGGAGAGTTCCCGCCAATTTCTGTTTTCCCCGGTGGGTCTCTACCCATGTTTTGCCAGTTTGCAGCGGTTGGTACCAACTTGGTGGTGGTTGGCGGTTTAGACCCGGTGACCTGGGCGAGCTGTAAATCGGTCTTCGTTTACAACTTCATGACTGCCACGTGGCGGCGCGGAACCGATATGCCGGGTGGGTCCCGCACATTTTTTGGGTGTGCGTCGGACTCAAACCGGATGGTGTTTGTCGCCGGCGGACACGATGGCGAGAAGAATGCATTGAGATCGGCGATGGCGTATGACGTGGCAAAAGACGAGTGGATTCTATTGCCTAACATGGAAAGAGAGCGCGACGAATGCAAGGTAGTTTTTCAGCATGGTAAGCTCCACGTCATTGGTGGCTATTGCACCGAAATGCAAGGCCGCTTTGAGAGAAGCGCTGAGACATTCAACACCTCCACATGGCAGTGGGATTGTGTACAAGAGGACTTCTTACAGATCAGCATGTGTCCACGGACGTGTGTCGCTGGCGATGACGGGGCAATGTACATGTGTCGCGATGGTAATGTGATCAAACAAACATGTGGCATGTGGCAAACCGTGGCCGAGCTGCCAGCTCATGTGCGCAACCCGGCTTCACTGACAGCGTGGCACGGTAAGCTATTAGTGATTGGATGTGCCGGGTTCGGTGAGCCCCACATGGCTTACATGTTTAATCTGGAAAATCACACCTGGAGTAAGGTAGATACCCCTGAGAAGTACTCTGGTCATGTACAATCAAGTTGCTATTTGGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

358

Amino Acids

39.9

Weight (kDa)

6.15

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 108 - 202 1.4e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 115 - 156 5.8e-08 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 115 - 256 8.8e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 121 - 260 7.6e-08 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 123 - 253 4.1e-10 Calicin, beta-propeller domain
Kelch_1 PF01344 170 - 205 3e-07 Kelch motif
Kelch_FKB95 PF25210 186 - 282 1.3e-07 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 902
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 36
AccII CGCG 3 cut(s) 453, 627, 834
AciI CCGC 9 cut(s) 242, 296, 350, 378, 448, 453, 478, 531, 700
AcoI YGGCCR 2 cut(s) 157, 879
AcuI CTGAAG 1 cut(s) 121
AcvI CACGTG 2 cut(s) 188, 444
AfaI GTAC 6 cut(s) 248, 358, 756, 824, 1037, 1050
AfeI AGCGCT 1 cut(s) 715
AfiI CCNNNNNNNGG 4 cut(s) 203, 275, 447, 738
AflIII ACRYGT 5 cut(s) 187, 794, 825, 857, 984
AgeI ACCGGT 1 cut(s) 249
AgsI TTSAA 3 cut(s) 182, 226, 727
AjiI CACGTC 3 cut(s) 580, 667, 795
AjnI CCWGG 2 cut(s) 395, 1007
AleI CACNNNNGTG 1 cut(s) 793
AluBI AGCT 7 cut(s) 118, 166, 405, 661, 886, 893, 937
AluI AGCT 7 cut(s) 118, 166, 405, 661, 886, 893, 937
Alw26I GTCTC 2 cut(s) 327, 713
Aor51HI AGCGCT 1 cut(s) 715
AoxI GGCC 3 cut(s) 157, 697, 879
ApeKI GCWGC 2 cut(s) 347, 886
AsiGI ACCGGT 1 cut(s) 249
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 4 cut(s) 453, 627, 716, 903
AspS9I GGNCC 1 cut(s) 474
AsuC2I CCSGG 5 cut(s) 315, 389, 468, 908, 958
AsuHPI GGTGA 4 cut(s) 247, 290, 403, 977
AvaII GGWCC 1 cut(s) 474
BaeI ACNNNNGTAYC 2 cut(s) 230, 263
BalI TGGCCA 1 cut(s) 159
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 972
BbrPI CACGTG 2 cut(s) 188, 444
BbsI GAAGAC 3 cut(s) 11, 214, 409
BbvI GCAGC 2 cut(s) 359, 873
BccI CCATC 5 cut(s) 169, 508, 533, 562, 830
BcgI CGANNNNNNTGC 4 cut(s) 533, 543, 567, 577
BciT130I CCWGG 2 cut(s) 397, 1009
BclI TGATCA 2 cut(s) 64, 846
BcnI CCSGG 5 cut(s) 315, 389, 468, 908, 958
BcoDI GTCTC 2 cut(s) 327, 713
BfoI RGCGCY 1 cut(s) 717
BisI GCNGC 4 cut(s) 348, 449, 700, 887
BlsI GCNGC 4 cut(s) 349, 450, 701, 888
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 7 cut(s) 315, 389, 397, 468, 908, 958, 1009
Bme18I GGWCC 1 cut(s) 474
BmgBI CACGTC 3 cut(s) 580, 667, 795
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 4 cut(s) 358, 457, 475, 476
BmrFI CCNGG 7 cut(s) 315, 389, 397, 468, 908, 958, 1009
BoxI GACNNNNGTC 1 cut(s) 797
BpiI GAAGAC 3 cut(s) 11, 214, 409
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpmI CTGGAG 1 cut(s) 1030
BpuMI CCSGG 5 cut(s) 315, 389, 468, 908, 958
BsaAI YACGTR 2 cut(s) 188, 444
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 202, 313, 396, 788, 876
BsaWI WCCGGW 2 cut(s) 249, 510
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 275, 447, 738
Bse118I RCCGGY 2 cut(s) 249, 527
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 825
BseBI CCWGG 2 cut(s) 397, 1009
BseDI CCNNGG 5 cut(s) 202, 313, 396, 788, 876
BseGI GGATG 2 cut(s) 519, 956
BseLI CCNNNNNNNGG 4 cut(s) 203, 275, 447, 738
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 2 cut(s) 708, 1020
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 2 cut(s) 359, 873
Bsh1236I CGCG 3 cut(s) 453, 627, 834
BshFI GGCC 3 cut(s) 159, 699, 881
BshNI GGYRCC 1 cut(s) 356
BshTI ACCGGT 1 cut(s) 249
BsiSI CCGG 9 cut(s) 250, 269, 315, 389, 467, 511, 528, 908, 957
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 4 cut(s) 203, 275, 447, 738
BsmAI GTCTC 2 cut(s) 327, 713
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 2 cut(s) 556, 638
BsnI GGCC 3 cut(s) 159, 699, 881
Bso31I GGTCTC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 972
Bsp1407I TGTACA 3 cut(s) 754, 822, 1048
Bsp143I GATC 4 cut(s) 64, 560, 774, 846
Bsp68I TCGCGA 1 cut(s) 834
BspACI CCGC 9 cut(s) 242, 296, 350, 378, 448, 453, 478, 531, 700
BspANI GGCC 3 cut(s) 159, 699, 881
BspCNI CTCAG 2 cut(s) 709, 1021
BspFNI CGCG 3 cut(s) 453, 627, 834
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 4 cut(s) 358, 457, 475, 476
BspT107I GGYRCC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 327
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 2 cut(s) 249, 527
BsrGI TGTACA 3 cut(s) 754, 822, 1048
BsrI ACTGG 1 cut(s) 340
BssAI RCCGGY 2 cut(s) 249, 527
BssECI CCNNGG 5 cut(s) 202, 313, 396, 788, 876
BssMI GATC 4 cut(s) 64, 560, 774, 846
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 397, 1009
Bst4CI ACNGT 3 cut(s) 259, 877, 932
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 3 cut(s) 754, 822, 1048
BstBAI YACGTR 2 cut(s) 188, 444
BstC8I GCNNGC 4 cut(s) 403, 529, 805, 891
BstDEI CTNAG 2 cut(s) 717, 1029
BstDSI CCRYGG 2 cut(s) 788, 876
BstEII GGTNACC 1 cut(s) 391
BstF5I GGATG 2 cut(s) 519, 956
BstFNI CGCG 3 cut(s) 453, 627, 834
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 4 cut(s) 453, 627, 716, 903
BstKTI GATC 4 cut(s) 67, 563, 777, 849
BstMAI GTCTC 2 cut(s) 327, 713
BstMBI GATC 4 cut(s) 64, 560, 774, 846
BstMWI GCNNNNNNNGC 4 cut(s) 191, 633, 658, 934
BstNI CCWGG 2 cut(s) 397, 1009
BstNSI RCATGY 5 cut(s) 784, 829, 861, 868, 988
BstPAI GACNNNNGTC 1 cut(s) 797
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 7 cut(s) 313, 387, 395, 466, 906, 956, 1007
BstUI CGCG 3 cut(s) 453, 627, 834
BstV1I GCAGC 2 cut(s) 359, 873
BstV2I GAAGAC 3 cut(s) 11, 214, 409
BstXI CCANNNNNNTGG 3 cut(s) 209, 367, 671
BsuRI GGCC 3 cut(s) 159, 699, 881
BtgI CCRYGG 2 cut(s) 788, 876
BtgZI GCGATG 4 cut(s) 116, 581, 822, 849
BtrI CACGTC 3 cut(s) 580, 667, 795
BtsCI GGATG 2 cut(s) 519, 956
BtsI GCAGTG 1 cut(s) 749
BtsIMutI CAGTG 2 cut(s) 749, 914
BtuMI TCGCGA 1 cut(s) 834
Cac8I GCNNGC 4 cut(s) 403, 529, 805, 891
CciI TCATGA 1 cut(s) 432
CfoI GCGC 4 cut(s) 453, 627, 716, 903
Cfr10I RCCGGY 2 cut(s) 249, 527
Cfr13I GGNCC 1 cut(s) 474
CseI GACGC 1 cut(s) 486
Csp6I GTAC 6 cut(s) 247, 357, 755, 823, 1036, 1049
CspAI ACCGGT 1 cut(s) 249
CspCI CAANNNNNGTGG 2 cut(s) 187, 222
CviQI GTAC 6 cut(s) 247, 357, 755, 823, 1036, 1049
DdeI CTNAG 2 cut(s) 717, 1029
DpnI GATC 4 cut(s) 66, 562, 776, 848
DpnII GATC 4 cut(s) 64, 560, 774, 846
EaeI YGGCCR 2 cut(s) 157, 879
EciI GGCGGA 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 972
Eco31I GGTCTC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 474
Eco47III AGCGCT 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 121
Eco72I CACGTG 2 cut(s) 188, 444
Eco91I GGTNACC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 395, 1007
EcoT14I CCWWGG 1 cut(s) 202
EcoT22I ATGCAT 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 972
ErhI CCWWGG 1 cut(s) 202
FaqI GGGAC 1 cut(s) 460
FauI CCCGC 2 cut(s) 303, 485
FbaI TGATCA 2 cut(s) 64, 846
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 4 cut(s) 348, 449, 700, 887
FokI GGATG 2 cut(s) 526, 963
FriOI GRGCYC 1 cut(s) 972
Fsp4HI GCNGC 4 cut(s) 348, 449, 700, 887
FspI TGCGCA 1 cut(s) 902
GlaI GCGC 4 cut(s) 452, 626, 715, 902
GluI GCNGC 4 cut(s) 348, 449, 700, 887
GsuI CTGGAG 1 cut(s) 1030
HaeII RGCGCY 1 cut(s) 717
HaeIII GGCC 3 cut(s) 159, 699, 881
HapII CCGG 9 cut(s) 250, 269, 315, 389, 467, 511, 528, 908, 957
HgaI GACGC 1 cut(s) 486
HhaI GCGC 4 cut(s) 453, 627, 716, 903
Hin6I GCGC 4 cut(s) 451, 625, 714, 901
HinP1I GCGC 4 cut(s) 451, 625, 714, 901
HinfI GANTC 3 cut(s) 272, 503, 598
HpaII CCGG 9 cut(s) 250, 269, 315, 389, 467, 511, 528, 908, 957
HphI GGTGA 4 cut(s) 247, 290, 403, 977
Hpy166II GTNNAC 5 cut(s) 37, 255, 424, 755, 788
Hpy188I TCNGA 4 cut(s) 75, 109, 140, 502
Hpy188III TCNNGA 4 cut(s) 23, 433, 833, 995
Hpy8I GTNNAC 5 cut(s) 37, 255, 424, 755, 788
Hpy99I CGWCG 2 cut(s) 502, 632
HpyCH4III ACNGT 3 cut(s) 259, 877, 932
HpyCH4IV ACGT 5 cut(s) 187, 443, 579, 666, 794
HpyCH4V TGCA 5 cut(s) 347, 554, 636, 684, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 633, 658, 934
HpyF3I CTNAG 2 cut(s) 717, 1029
HpySE526I ACGT 5 cut(s) 187, 443, 579, 666, 794
HspAI GCGC 4 cut(s) 451, 625, 714, 901
KflI GGGWCCC 1 cut(s) 474
KpnI GGTACC 1 cut(s) 360
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 64, 846
Kzo9I GATC 4 cut(s) 64, 560, 774, 846
LmnI GCTCC 2 cut(s) 199, 666
Lsp1109I GCAGC 2 cut(s) 359, 873
MaeII ACGT 5 cut(s) 187, 443, 579, 666, 794
MaeIII GTNAC 1 cut(s) 391
MalI GATC 4 cut(s) 66, 562, 776, 848
MboI GATC 4 cut(s) 64, 560, 774, 846
MboII GAAGA 4 cut(s) 11, 214, 409, 559
MhlI GDGCHC 1 cut(s) 972
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 1 cut(s) 300
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 80, 98, 480
MnlI CCTC 3 cut(s) 103, 742, 754
Mox20I TGGCCA 1 cut(s) 159
Mph1103I ATGCAT 1 cut(s) 556
MreI CGCCGGCG 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 527
MscI TGGCCA 1 cut(s) 159
MseI TTAA 1 cut(s) 990
MslI CAYNNNNRTG 4 cut(s) 173, 689, 742, 793
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 2 cut(s) 244, 350
MspI CCGG 9 cut(s) 250, 269, 315, 389, 467, 511, 528, 908, 957
MspR9I CCNGG 7 cut(s) 315, 389, 397, 468, 908, 958, 1009
Mva1269I GAATGC 2 cut(s) 556, 638
MvaI CCWGG 2 cut(s) 397, 1009
MvnI CGCG 3 cut(s) 453, 627, 834
MwoI GCNNNNNNNGC 4 cut(s) 191, 633, 658, 934
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 5 cut(s) 315, 389, 468, 908, 958
NdeII GATC 4 cut(s) 64, 560, 774, 846
NgoMIV GCCGGC 1 cut(s) 527
NlaIV GGNNCC 4 cut(s) 358, 457, 475, 476
NmeAIII GCCGAG 1 cut(s) 907
NmuCI GTSAC 1 cut(s) 391
NruI TCGCGA 1 cut(s) 834
NsbI TGCGCA 1 cut(s) 902
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 5 cut(s) 784, 829, 861, 868, 988
OliI CACNNNNGTG 1 cut(s) 793
PagI TCATGA 1 cut(s) 432
PciI ACATGT 3 cut(s) 825, 857, 984
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PctI GAATGC 2 cut(s) 556, 638
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 272, 598
PinAI ACCGGT 1 cut(s) 249
PkrI GCNGC 4 cut(s) 349, 450, 701, 888
PleI GAGTC 1 cut(s) 497
PmaCI CACGTG 2 cut(s) 188, 444
PmlI CACGTG 2 cut(s) 188, 444
PpsI GAGTC 1 cut(s) 497
Ppu21I YACGTR 2 cut(s) 188, 444
PpuMI RGGWCCY 1 cut(s) 474
PscI ACATGT 3 cut(s) 825, 857, 984
PshAI GACNNNNGTC 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 395, 1007
PspCI CACGTG 2 cut(s) 188, 444
PspEI GGTNACC 1 cut(s) 391
PspGI CCWGG 2 cut(s) 395, 1007
PspN4I GGNNCC 4 cut(s) 358, 457, 475, 476
PspPI GGNCC 1 cut(s) 474
PspPPI RGGWCCY 1 cut(s) 474
RruI TCGCGA 1 cut(s) 834
RsaI GTAC 6 cut(s) 248, 358, 756, 824, 1037, 1050
RsaNI GTAC 6 cut(s) 247, 357, 755, 823, 1036, 1049
RseI CAYNNNNRTG 4 cut(s) 173, 689, 742, 793
SaqAI TTAA 1 cut(s) 990
SatI GCNGC 4 cut(s) 348, 449, 700, 887
Sau3AI GATC 4 cut(s) 64, 560, 774, 846
Sau96I GGNCC 1 cut(s) 474
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 7 cut(s) 315, 389, 397, 468, 908, 958, 1009
SduI GDGCHC 1 cut(s) 972
SgrAI CRCCGGYG 1 cut(s) 527
SinI GGWCC 1 cut(s) 474
SmiMI CAYNNNNRTG 4 cut(s) 173, 689, 742, 793
Sse9I AATT 1 cut(s) 300
SsiI CCGC 9 cut(s) 242, 296, 350, 378, 448, 453, 478, 531, 700
StyD4I CCNGG 7 cut(s) 313, 387, 395, 466, 906, 956, 1007
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 3 cut(s) 259, 877, 932
TaiI ACGT 5 cut(s) 190, 446, 582, 669, 797
TaqII GACCGA 1 cut(s) 403
TasI AATT 1 cut(s) 300
TatI WGTACW 4 cut(s) 754, 822, 1035, 1048
TauI GCSGC 2 cut(s) 451, 702
TfiI GAWTC 2 cut(s) 272, 598
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 2 cut(s) 749, 921
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 2 cut(s) 347, 886
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 3 cut(s) 42, 119, 421
TspGWI ACGGA 1 cut(s) 805
TspRI CASTG 2 cut(s) 749, 921
VpaK11BI GGWCC 1 cut(s) 474
XceI RCATGY 5 cut(s) 784, 829, 861, 868, 988
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 671
XmiI GTMKAC 1 cut(s) 36
ZrmI AGTACT 1 cut(s) 1037
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.