FvH4_6g41510

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003575
Physical Location & Seq
Forward (+)
32618956 .. 32622104
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41510.t1

Sequence Viewer

Length: 684 bp
ATGGGTGGAGGGCAGAAGAGTGACAAGGAAAAAGTGGAGGAAGAAGAAACTGATAATGAAGCAGGTGAGAGAATTACCAGACAGATGAGCGAGACCTCTGTTTCTGCAGCTGAGGATGATGATGATGAAGAAAACAGCAATAAAATCCAGTTGGGTCCTCAATGTACTCTCAAAGAACAGATTGAGAAGGATAAGGATGATGAGAGCTTGAGGAGGTGGAAAGAACAGCTTCTTGGGGCTGTGGATTTTGAGAGTGTTGGAGAAACTCTTGACCCAGAAGTAAAGATTAATAGCCTCTCAATCCTCACTCCTGACAGACCCGACATTGTTCTTGAAGTTCCGGAAAATGGAAAACCCAAAGGCTTATGGTTTACCCTGAAGGAAGGTAGTCGTCACAACTTGAAATTCTCCTTCCAAGTCAGCAATAACATTGTATCCGGCCTCAAGTACACCAACACTGTTTGGAAAACTGGTGTCAAGGTGGACAGCACAAAAGAGATGATTGGAACCTTCAGTCCTCAGCCAGAGCCATACACACATGTGATGCCAGAAGAGGTCACACCTTCCGGCATGTTTGCCAGAGGATCATACTCTGCAAGATCAAAGTTTCTCGACGATGATAACAAGTGCTACTTGGAAATCAATTACACCTTCGATATTCGAAAGGAATGGGCTGCGCGCTAG

Protein Analysis

228

Amino Acids

25.74

Weight (kDa)

4.7

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 37 - 223 8.7e-88 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 53
AccII CGCG 1 cut(s) 679
AccIII TCCGGA 1 cut(s) 340
AclWI GGATC 1 cut(s) 592
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 398, 496
AfaI GTAC 2 cut(s) 166, 449
AfiI CCNNNNNNNGG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 2 cut(s) 335, 403
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AleI CACNNNNGTG 1 cut(s) 539
AloI GAACNNNNNNTCC 2 cut(s) 499, 531
AluBI AGCT 3 cut(s) 110, 207, 229
AluI AGCT 3 cut(s) 110, 207, 229
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 592
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 2 cut(s) 107, 674
ApoI RAATTY 1 cut(s) 404
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 2 cut(s) 679, 681
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 77
AsuII TTCGAA 1 cut(s) 661
AvaII GGWCC 1 cut(s) 155
BbvCI CCTCAGC 2 cut(s) 111, 519
BbvI GCAGC 2 cut(s) 119, 661
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 682
BfmI CTRYAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 53
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 2 cut(s) 108, 675
BlsI GCNGC 2 cut(s) 109, 676
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 2 cut(s) 156, 508
BmsI GCATC 1 cut(s) 534
Bpu10I CCTNAGC 2 cut(s) 111, 519
Bpu14I TTCGAA 1 cut(s) 661
BpuEI CTTGAG 2 cut(s) 229, 428
BsaI GGTCTC 1 cut(s) 86
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 148, 475
BseAI TCCGGA 1 cut(s) 340
BseGI GGATG 2 cut(s) 121, 202
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 2 cut(s) 102, 533
BseNI ACTGG 2 cut(s) 148, 475
BsePI GCGCGC 1 cut(s) 677
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 2 cut(s) 119, 661
Bsh1236I CGCG 1 cut(s) 679
BshFI GGCC 1 cut(s) 441
BsiSI CCGG 3 cut(s) 341, 438, 567
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 1 cut(s) 441
Bso31I GGTCTC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 661
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 2 cut(s) 584, 599
BspANI GGCC 1 cut(s) 441
BspCNI CTCAG 2 cut(s) 103, 532
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 1 cut(s) 679
BspLI GGNNCC 2 cut(s) 156, 508
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 1 cut(s) 592
BspT104I TTCGAA 1 cut(s) 661
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 2 cut(s) 148, 475
BssHII GCGCGC 1 cut(s) 677
BssMI GATC 2 cut(s) 584, 599
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 2 cut(s) 11, 546
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 2 cut(s) 111, 519
BstF5I GGATG 2 cut(s) 121, 202
BstFNI CGCG 1 cut(s) 679
BstHHI GCGC 2 cut(s) 679, 681
BstKTI GATC 2 cut(s) 587, 602
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 584, 599
BstNSI RCATGY 2 cut(s) 542, 574
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 2 cut(s) 119, 661
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 1 cut(s) 441
BtsCI GGATG 2 cut(s) 121, 202
BtsIMutI CAGTG 1 cut(s) 456
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 1 cut(s) 679
CfoI GCGC 2 cut(s) 679, 681
Cfr13I GGNCC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 165, 448
CviAII CATG 2 cut(s) 539, 571
CviQI GTAC 2 cut(s) 165, 448
DdeI CTNAG 2 cut(s) 111, 519
DpnI GATC 2 cut(s) 586, 601
DpnII GATC 2 cut(s) 584, 599
Eam1104I CTCTTC 2 cut(s) 11, 546
EarI CTCTTC 2 cut(s) 11, 546
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 398, 496
EcoO109I RGGNCCY 1 cut(s) 155
FaeI CATG 2 cut(s) 542, 574
FaiI YATR 5 cut(s) 367, 532, 540, 572, 589
FalI AAGNNNNNCTT 4 cut(s) 213, 245, 617, 649
FatI CATG 2 cut(s) 538, 570
Fnu4HI GCNGC 2 cut(s) 108, 675
FokI GGATG 2 cut(s) 128, 209
Fsp4HI GCNGC 2 cut(s) 108, 675
FspBI CTAG 1 cut(s) 682
GlaI GCGC 2 cut(s) 678, 680
GluI GCNGC 2 cut(s) 108, 675
HaeIII GGCC 1 cut(s) 441
HapII CCGG 3 cut(s) 341, 438, 567
HhaI GCGC 2 cut(s) 679, 681
Hin1II CATG 2 cut(s) 542, 574
Hin6I GCGC 2 cut(s) 677, 679
HinP1I GCGC 2 cut(s) 677, 679
HpaII CCGG 3 cut(s) 341, 438, 567
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 372, 450, 484
Hpy188III TCNNGA 5 cut(s) 269, 311, 332, 341, 611
Hpy8I GTNNAC 3 cut(s) 372, 450, 484
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 7 cut(s) 181, 373, 377, 421, 520, 573, 661
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 2 cut(s) 107, 596
HpyF3I CTNAG 2 cut(s) 111, 519
Hsp92II CATG 2 cut(s) 542, 574
HspAI GCGC 2 cut(s) 677, 679
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 2 cut(s) 584, 599
Lsp1109I GCAGC 2 cut(s) 119, 661
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 682
MaeIII GTNAC 3 cut(s) 20, 392, 556
MalI GATC 2 cut(s) 586, 601
MboI GATC 2 cut(s) 584, 599
MboII GAAGA 5 cut(s) 28, 53, 56, 140, 563
MluCI AATT 3 cut(s) 72, 404, 643
MmeI TCCRAC 1 cut(s) 238
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 341, 438, 567
MvnI CGCG 1 cut(s) 679
NdeII GATC 2 cut(s) 584, 599
NlaIII CATG 2 cut(s) 542, 574
NlaIV GGNNCC 2 cut(s) 156, 508
NmuCI GTSAC 3 cut(s) 20, 392, 556
NspI RCATGY 2 cut(s) 542, 574
NspV TTCGAA 1 cut(s) 661
OliI CACNNNNGTG 1 cut(s) 539
PaqCI CACCTGC 1 cut(s) 53
PauI GCGCGC 1 cut(s) 677
PciI ACATGT 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 228
PkrI GCNGC 2 cut(s) 109, 676
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 538
PshBI ATTAAT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 155
PspN4I GGNNCC 2 cut(s) 156, 508
PspPI GGNCC 1 cut(s) 155
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 109
PteI GCGCGC 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 166, 449
RsaNI GTAC 2 cut(s) 165, 448
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 2 cut(s) 108, 675
Sau3AI GATC 2 cut(s) 584, 599
Sau96I GGNCC 1 cut(s) 155
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 208, 443
SmoI CTYRAG 2 cut(s) 208, 443
Sse9I AATT 3 cut(s) 72, 404, 643
SspMI CTAG 1 cut(s) 682
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 3 cut(s) 612, 654, 661
TasI AATT 3 cut(s) 72, 404, 643
TatI WGTACW 2 cut(s) 164, 447
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 3 cut(s) 20, 392, 556
TseI GCWGC 2 cut(s) 107, 674
Tsp45I GTSAC 3 cut(s) 20, 392, 556
TspDTI ATGAA 2 cut(s) 72, 141
TspRI CASTG 1 cut(s) 463
VpaK11BI GGWCC 1 cut(s) 155
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 1 cut(s) 404
XceI RCATGY 2 cut(s) 542, 574
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.