Rh2DG547000

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003575
Physical Location & Seq
Forward (+)
77567311 .. 77571150
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG547000.1

Sequence Viewer

Length: 645 bp
ATGGGTGGGGGACAGAAGAGTGACAAAGAAAAAGTGGAGGAAGAAGATACTGATAATGAAGCAGGAGAGGGACTTCACAGGCAGATGAGTGAGACTTCTGTTTCTGCAGCTGAGGATGATGATGATGAAGAAAACAGCAACAAGATTCAGTTGGGTCCTCAATGCACTCTCAAAGAACAGATTGAGAAGGATAAGGATGATGAGAGCTTGAGGAGGTGGAAGGAACAGCTTCTTGGGGCTGTGGATTTTGAGAGTGTTGGAGAAACTCTAGACCCAGAAGTAAAGATTATTAGCCTCTCAATCCTCACTCCTGATAGACCTGACATTGTTCTTGATGTTCCGGACAATGGAAAACCCAAAGGCTTGTGGTTTACTCTGAAGGAAGGTAGTCCTCACAACTTGAAATTCTCCTTCCGAGTTAGCAATAACATTGTATCTGGCCTCAAGTACACCAATACTGTTTGGAAAACCGGCGTCAAGGTGGACAGCACAAAAGAGATGATTGGAACCTTCAGTCCTCAGCCAGAGCCATACACACATGTGATGCCAGAAGTGGTCACACCTTCCGGCATGTTTGCAAGAGGATCATATTCTGCAAGATCAAAGGTAAACCTGGACCAGTTAAAGTTACTTGCACAAATCTAA

Protein Analysis

214

Amino Acids

23.8

Weight (kDa)

4.74

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 37 - 206 4.3e-73 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 340
AclWI GGATC 1 cut(s) 592
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 398, 496
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 612
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AleI CACNNNNGTG 1 cut(s) 539
AloI GAACNNNNNNTCC 2 cut(s) 499, 531
AluBI AGCT 3 cut(s) 110, 207, 229
AluI AGCT 3 cut(s) 110, 207, 229
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 592
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 155, 616
AvaII GGWCC 2 cut(s) 155, 616
BbvCI CCTCAGC 2 cut(s) 111, 519
BbvI GCAGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 614
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 614
Bme18I GGWCC 2 cut(s) 155, 616
BmgT120I GGNCC 2 cut(s) 155, 616
BmiI GGNNCC 2 cut(s) 156, 508
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 534
Bpu10I CCTNAGC 2 cut(s) 111, 519
BpuEI CTTGAG 2 cut(s) 229, 428
BsaHI GRCGYC 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 619
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 1 cut(s) 614
BseGI GGATG 2 cut(s) 121, 202
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 2 cut(s) 102, 533
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 1 cut(s) 441
BsiSI CCGG 3 cut(s) 341, 471, 567
BslFI GGGAC 2 cut(s) 24, 84
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 2 cut(s) 24, 84
BsnI GGCC 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 2 cut(s) 584, 599
BspANI GGCC 1 cut(s) 441
BspCNI CTCAG 2 cut(s) 103, 532
BspEI TCCGGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 156, 508
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 592
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 1 cut(s) 619
BssAI RCCGGY 1 cut(s) 470
BssMI GATC 2 cut(s) 584, 599
BssNI GRCGYC 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 111, 519
BstF5I GGATG 2 cut(s) 121, 202
BstKTI GATC 2 cut(s) 587, 602
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 584, 599
BstNI CCWGG 1 cut(s) 614
BstNSI RCATGY 2 cut(s) 542, 574
BstSCI CCNGG 1 cut(s) 612
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 119
BsuRI GGCC 1 cut(s) 441
BtsCI GGATG 2 cut(s) 121, 202
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 155, 616
CseI GACGC 1 cut(s) 463
Csp6I GTAC 1 cut(s) 448
CviAII CATG 2 cut(s) 539, 571
CviJI RGCY 9 cut(s) 110, 207, 229, 239, 294, 363, 441, 523, 529
CviKI_1 RGCY 9 cut(s) 110, 207, 229, 239, 294, 363, 441, 523, 529
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 2 cut(s) 111, 519
DpnI GATC 2 cut(s) 586, 601
DpnII GATC 2 cut(s) 584, 599
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 155, 616
Eco57I CTGAAG 2 cut(s) 398, 496
EcoO109I RGGNCCY 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 612
FaeI CATG 2 cut(s) 542, 574
FaiI YATR 4 cut(s) 532, 540, 572, 589
FaqI GGGAC 2 cut(s) 24, 84
FatI CATG 2 cut(s) 538, 570
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 128, 209
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 269
GluI GCNGC 1 cut(s) 108
HaeIII GGCC 1 cut(s) 441
HapII CCGG 3 cut(s) 341, 471, 567
HgaI GACGC 1 cut(s) 463
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 542, 574
HinfI GANTC 1 cut(s) 145
HpaII CCGG 3 cut(s) 341, 471, 567
Hpy166II GTNNAC 4 cut(s) 372, 450, 484, 610
Hpy188I TCNGA 2 cut(s) 378, 416
Hpy188III TCNNGA 4 cut(s) 269, 311, 332, 341
Hpy8I GTNNAC 4 cut(s) 372, 450, 484, 610
HpyAV CCTTC 7 cut(s) 181, 214, 373, 377, 421, 520, 573
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 5 cut(s) 107, 165, 578, 596, 635
HpyF3I CTNAG 2 cut(s) 111, 519
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 542, 574
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 2 cut(s) 584, 599
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 269
MaeIII GTNAC 3 cut(s) 20, 556, 627
MalI GATC 2 cut(s) 586, 601
MboI GATC 2 cut(s) 584, 599
MboII GAAGA 4 cut(s) 28, 53, 56, 140
MluCI AATT 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 238
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 1 cut(s) 623
MslI CAYNNNNRTG 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 341, 471, 567
MspR9I CCNGG 1 cut(s) 614
MvaI CCWGG 1 cut(s) 614
NdeII GATC 2 cut(s) 584, 599
NlaIII CATG 2 cut(s) 542, 574
NlaIV GGNNCC 2 cut(s) 156, 508
NmuCI GTSAC 2 cut(s) 20, 556
NspI RCATGY 2 cut(s) 542, 574
OliI CACNNNNGTG 1 cut(s) 539
PciI ACATGT 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 145
PkrI GCNGC 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 538
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 2 cut(s) 156, 508
PspPI GGNCC 2 cut(s) 155, 616
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 1 cut(s) 623
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 584, 599
Sau96I GGNCC 2 cut(s) 155, 616
ScrFI CCNGG 1 cut(s) 614
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 2 cut(s) 155, 616
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 2 cut(s) 208, 443
SmoI CTYRAG 2 cut(s) 208, 443
Sse9I AATT 1 cut(s) 404
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 1 cut(s) 612
TaaI ACNGT 1 cut(s) 460
TasI AATT 1 cut(s) 404
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 623
Tru9I TTAA 1 cut(s) 623
TseFI GTSAC 2 cut(s) 20, 556
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 2 cut(s) 20, 556
TspDTI ATGAA 2 cut(s) 72, 141
VpaK11BI GGWCC 2 cut(s) 155, 616
XapI RAATTY 1 cut(s) 404
XbaI TCTAGA 1 cut(s) 268
XceI RCATGY 2 cut(s) 542, 574
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.