MD10G1331800.v1.1

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003575
Physical Location & Seq
Reverse (-)
40947538 .. 40950094
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1331800.v1.1.491

Sequence Viewer

Length: 738 bp
ATGTCTCTGGCCGTTGGACTTGTTTCGAGCTCCAAAAACATGGGGTTGGATGATGAGAACGCCGCCGAGGTTTCCCACAAGACGGAAAAAACCGGTGAGGCAAGTGCGGATCAACTATCCACAGGGATGGTTAGATCACCCAGTGAGGGTTCCCTCTGTCAGACCGAGGAAGAAGACGACGATGACGAAGTAAAGAAGATTGAGTTGGGGCCTCAGTTCACTCTCAAAGAACAAATTGAGAAGGATGCGGACGATGAGAGCTTGAGGAGGTGGAAGGAACAGCTTCTTGGGAGTGTGGATGTTAATTCTGTTGGAGAAACTCTAGAACCAGAAGTTAAGATCATGAGCCTGGCAATTAAATCCCCTGGCAGATCTGATATAATTCTTCCGATTCCTGTGGACGGAAATCCCAAAGGGTTGTGGTTTACTTTGAAAGAAGGTAGCCGTTACAGCCTCGAATTCACTTTTCAGGTCAGCAATAACATCGTTTCAGGTCTCAAATACACCAACACAGTCTGGAAAACTGCTGTGAAAGTTGATAGCACGAAAGAAATGCTTGGAACATTTAGTCCACAGTCAGAGCCTTACACACATGTGTTGCCCGAGGACACAACCCCATCTGGCATATTTGCTAGGGGATCTTATTCAGCTCGAAGCAAGTTTGCTGACGATGACAACAAGTGCTTCTTGGAGATTAACTACACCTTTGATATCCGAAAAGACTGGCAATCGTCGTAA

Protein Analysis

246

Amino Acids

27.15

Weight (kDa)

4.6

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 55 - 241 1.5e-85 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 63, 107, 248
AclWI GGATC 2 cut(s) 117, 646
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 458
AdeI CACNNNGTG 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 82, 146, 401
AflIII ACRYGT 1 cut(s) 592
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 2 cut(s) 348, 364
AjuI GAANNNNNNNTTGG 4 cut(s) 188, 220, 270, 302
AleI CACNNNNGTG 1 cut(s) 593
AloI GAACNNNNNNTCC 2 cut(s) 553, 585
AluBI AGCT 4 cut(s) 30, 261, 283, 650
AluI AGCT 4 cut(s) 30, 261, 283, 650
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 9, 500
AlwI GGATC 2 cut(s) 117, 646
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 2 cut(s) 9, 209
ApoI RAATTY 1 cut(s) 458
AsiGI ACCGGT 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 107, 129
AvaI CYCGRG 1 cut(s) 602
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 32
BccI CCATC 2 cut(s) 121, 625
BceAI ACGGC 1 cut(s) 429
BcgI CGANNNNNNTGC 4 cut(s) 466, 500, 535, 569
BciT130I CCWGG 2 cut(s) 350, 366
BcoDI GTCTC 2 cut(s) 9, 500
BfaI CTAG 2 cut(s) 323, 633
BglII AGATCT 1 cut(s) 371
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 350, 366
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 151, 210
BmrFI CCNGG 2 cut(s) 350, 366
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 235
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 4 cut(s) 66, 165, 364, 603
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 146, 401
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 141, 728
BseBI CCWGG 2 cut(s) 350, 366
BseDI CCNNGG 4 cut(s) 66, 165, 364, 603
BseGI GGATG 4 cut(s) 55, 132, 250, 304
BseLI CCNNNNNNNGG 3 cut(s) 82, 146, 401
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 2 cut(s) 141, 728
BseRI GAGGAG 1 cut(s) 280
BshFI GGCC 2 cut(s) 11, 211
BshTI ACCGGT 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 82, 146, 401
BsmAI GTCTC 2 cut(s) 9, 500
BsnI GGCC 2 cut(s) 11, 211
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 602
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 5 cut(s) 109, 134, 339, 371, 638
BspACI CCGC 3 cut(s) 63, 107, 248
BspANI GGCC 2 cut(s) 11, 211
BspCNI CTCAG 1 cut(s) 226
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 151, 210
BspPI GGATC 2 cut(s) 117, 646
BspTNI GGTCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 141, 728
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 4 cut(s) 66, 165, 364, 603
BssMI GATC 5 cut(s) 109, 134, 339, 371, 638
Bst2UI CCWGG 2 cut(s) 350, 366
Bst4CI ACNGT 2 cut(s) 514, 576
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 4 cut(s) 55, 132, 250, 304
BstKTI GATC 5 cut(s) 112, 137, 342, 374, 641
BstMAI GTCTC 2 cut(s) 9, 500
BstMBI GATC 5 cut(s) 109, 134, 339, 371, 638
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNI CCWGG 2 cut(s) 350, 366
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 348, 364
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 2 cut(s) 371, 638
BstXI CCANNNNNNTGG 2 cut(s) 40, 127
BstYI RGATCY 2 cut(s) 371, 638
BsuRI GGCC 2 cut(s) 11, 211
BtsCI GGATG 4 cut(s) 55, 132, 250, 304
BtsIMutI CAGTG 1 cut(s) 148
CciI TCATGA 1 cut(s) 342
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 209
CspAI ACCGGT 1 cut(s) 92
CviAII CATG 3 cut(s) 40, 343, 593
DdeI CTNAG 1 cut(s) 213
DpnI GATC 5 cut(s) 111, 136, 341, 373, 640
DpnII GATC 5 cut(s) 109, 134, 339, 371, 638
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 712
Eco53kI GAGCTC 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 348, 364
EcoRV GATATC 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 3 cut(s) 43, 346, 596
FaiI YATR 5 cut(s) 41, 344, 380, 594, 626
FalI AAGNNNNNCTT 4 cut(s) 540, 572, 671, 703
FatI CATG 3 cut(s) 39, 342, 592
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 4 cut(s) 62, 139, 257, 311
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 323, 633
GluI GCNGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 11, 211
HapII CCGG 1 cut(s) 93
Hin1II CATG 3 cut(s) 43, 346, 596
HinfI GANTC 1 cut(s) 391
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 107, 129
Hpy166II GTNNAC 4 cut(s) 219, 400, 426, 572
Hpy188I TCNGA 5 cut(s) 162, 376, 390, 580, 716
Hpy188III TCNNGA 3 cut(s) 323, 343, 517
Hpy8I GTNNAC 4 cut(s) 219, 400, 426, 572
Hpy99I CGWCG 2 cut(s) 182, 736
HpyAV CCTTC 3 cut(s) 235, 268, 431
HpyCH4III ACNGT 2 cut(s) 514, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 1 cut(s) 213
Hsp92II CATG 3 cut(s) 43, 346, 596
Kzo9I GATC 5 cut(s) 109, 134, 339, 371, 638
LmnI GCTCC 1 cut(s) 35
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 323, 633
MaeIII GTNAC 1 cut(s) 446
MalI GATC 5 cut(s) 111, 136, 341, 373, 640
MboI GATC 5 cut(s) 109, 134, 339, 371, 638
MboII GAAGA 4 cut(s) 182, 185, 208, 377
MflI RGATCY 2 cut(s) 371, 638
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 5 cut(s) 234, 304, 354, 381, 458
MmeI TCCRAC 2 cut(s) 27, 292
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 4 cut(s) 303, 336, 357, 696
MslI CAYNNNNRTG 2 cut(s) 125, 593
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 350, 366
MvaI CCWGG 2 cut(s) 350, 366
MwoI GCNNNNNNNGC 1 cut(s) 450
NdeII GATC 5 cut(s) 109, 134, 339, 371, 638
NlaIII CATG 3 cut(s) 43, 346, 596
NlaIV GGNNCC 2 cut(s) 151, 210
NmeAIII GCCGAG 1 cut(s) 91
NspI RCATGY 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 593
PagI TCATGA 1 cut(s) 342
PciI ACATGT 1 cut(s) 592
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 391
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 1 cut(s) 64
PscI ACATGT 1 cut(s) 592
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 348, 364
PspGI CCWGG 2 cut(s) 348, 364
PspN4I GGNNCC 2 cut(s) 151, 210
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 2 cut(s) 371, 638
RseI CAYNNNNRTG 2 cut(s) 125, 593
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 4 cut(s) 303, 336, 357, 696
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 5 cut(s) 109, 134, 339, 371, 638
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 350, 366
SduI GDGCHC 1 cut(s) 32
SfaNI GCATC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 125, 593
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
Sse9I AATT 5 cut(s) 234, 304, 354, 381, 458
SsiI CCGC 3 cut(s) 63, 107, 248
SspMI CTAG 2 cut(s) 323, 633
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 348, 364
TaaI ACNGT 2 cut(s) 514, 576
TaqI TCGA 3 cut(s) 26, 456, 652
TaqII GACCGA 1 cut(s) 179
TasI AATT 5 cut(s) 234, 304, 354, 381, 458
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 303, 336, 357, 696
Tru9I TTAA 4 cut(s) 303, 336, 357, 696
TscAI CASTG 1 cut(s) 148
TspGWI ACGGA 2 cut(s) 98, 417
TspRI CASTG 1 cut(s) 148
XapI RAATTY 1 cut(s) 458
XbaI TCTAGA 1 cut(s) 322
XceI RCATGY 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 2 cut(s) 323, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.