MD17G1110100.v1.1

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003575
Physical Location & Seq
Reverse (-)
9489041 .. 9492684
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1110100.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGGGGAGGAGAGAAGAGTGACAAAGAAACAAAGAAAGAAAACCAGGATGATACAGATAATGAAGGAGTGGAGGGGATTGGGAGGCAGATGAGTGAGACTTCTGTTTCTGCGGCTGAGGAGGAGGAAGACGAAGAGGGAAGCAGTAAAATTCAGTTGGGTCCCCAATGCACTCTCAAAGAACAGATTGAGAAGGATAAGGATGATGAGAGTTTGAGGAGGTGGAAGGAGCAGCTTCTTGGGGCTGTGGATTTTGAGAATGTTGGAGAAACTCTTGAACCAGAAGTGAAGTTCATTCAGCTTTCAATCCTTTCTCCTGGTAGACCTGACATTGTTCTTGATATTCCGGGAGATGGGAAACCCAAAGGCTTATGGTTCACGCTGAAAGAAGGTAGTCCGCACAACCTGAAGTTCTCCTTCCAAGTTAGCAATAACATCGTATCCGGTCTCAAGTACACCAACACTGTTTGGAAAACTGGTGTCAAGGTCGACAGCACAAAAGAGATGATTGGAACTTTCAGTCCTCAGCAAGAGCCTTACATACATGTGATGCCAGAAGAGACCACACCTTCTGGCATGTTTGCCAGAGGATCATATTCTGCAAGATCGAAGTTTCTTGACGATGATAACAAGTGTTACTTGGAGATCAATTACACCTTCGATATTCGAAAGGAATGGGCTAAATAA

Protein Analysis

229

Amino Acids

25.65

Weight (kDa)

4.69

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 39 - 225 3.1e-87 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 322, 489
AciI CCGC 2 cut(s) 113, 398
AclWI GGATC 1 cut(s) 598
AcsI RAATTY 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 2 cut(s) 278, 306
AjnI CCWGG 2 cut(s) 45, 316
AloI GAACNNNNNNTCC 2 cut(s) 505, 537
AluBI AGCT 2 cut(s) 235, 301
AluI AGCT 2 cut(s) 235, 301
Alw26I GTCTC 3 cut(s) 92, 452, 554
AlwI GGATC 1 cut(s) 598
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 1 cut(s) 150
AspS9I GGNCC 1 cut(s) 161
AsuC2I CCSGG 1 cut(s) 348
AsuII TTCGAA 1 cut(s) 667
AvaII GGWCC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 135
BbvCI CCTCAGC 2 cut(s) 117, 525
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 347
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 2 cut(s) 47, 318
BciVI GTATCC 1 cut(s) 451
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 3 cut(s) 92, 452, 554
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 2 cut(s) 114, 233
BlsI GCNGC 2 cut(s) 115, 234
Bme1390I CCNGG 3 cut(s) 47, 318, 348
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 162, 163
BmrFI CCNGG 3 cut(s) 47, 318, 348
BmsI GCATC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 135
Bpu10I CCTNAGC 2 cut(s) 117, 525
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 348
BsaI GGTCTC 2 cut(s) 452, 554
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 481
BseBI CCWGG 2 cut(s) 47, 318
BseGI GGATG 2 cut(s) 55, 208
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 108, 539
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 4 cut(s) 24, 134, 137, 232
BseXI GCAGC 1 cut(s) 244
BsiSI CCGG 2 cut(s) 347, 444
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 3 cut(s) 92, 452, 554
BsmFI GGGAC 1 cut(s) 147
Bso31I GGTCTC 2 cut(s) 452, 554
Bsp119I TTCGAA 1 cut(s) 667
Bsp143I GATC 3 cut(s) 590, 605, 645
BspACI CCGC 2 cut(s) 113, 398
BspCNI CTCAG 2 cut(s) 109, 538
BspLI GGNNCC 2 cut(s) 162, 163
BspPI GGATC 1 cut(s) 598
BspT104I TTCGAA 1 cut(s) 667
BspTNI GGTCTC 2 cut(s) 452, 554
BsrI ACTGG 1 cut(s) 481
BssMI GATC 3 cut(s) 590, 605, 645
Bst2UI CCWGG 2 cut(s) 47, 318
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 3 cut(s) 11, 129, 552
BstBI TTCGAA 1 cut(s) 667
BstDEI CTNAG 2 cut(s) 117, 525
BstF5I GGATG 2 cut(s) 55, 208
BstKTI GATC 3 cut(s) 593, 608, 648
BstMAI GTCTC 3 cut(s) 92, 452, 554
BstMBI GATC 3 cut(s) 590, 605, 645
BstNI CCWGG 2 cut(s) 47, 318
BstNSI RCATGY 2 cut(s) 548, 580
BstSCI CCNGG 3 cut(s) 45, 316, 346
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 135
BsuI GTATCC 1 cut(s) 451
BtsCI GGATG 2 cut(s) 55, 208
BtsIMutI CAGTG 1 cut(s) 462
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 545, 577
CviJI RGCY 7 cut(s) 116, 235, 245, 301, 369, 535, 680
CviKI_1 RGCY 7 cut(s) 116, 235, 245, 301, 369, 535, 680
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 2 cut(s) 117, 525
DpnI GATC 3 cut(s) 592, 607, 647
DpnII GATC 3 cut(s) 590, 605, 645
Eam1104I CTCTTC 3 cut(s) 11, 129, 552
EarI CTCTTC 3 cut(s) 11, 129, 552
Eco31I GGTCTC 2 cut(s) 452, 554
Eco47I GGWCC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 45, 316
FaeI CATG 2 cut(s) 548, 580
FaiI YATR 5 cut(s) 373, 542, 546, 578, 595
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 623, 655
FaqI GGGAC 1 cut(s) 147
FatI CATG 2 cut(s) 544, 576
FblI GTMKAC 2 cut(s) 322, 489
Fnu4HI GCNGC 2 cut(s) 114, 233
FokI GGATG 2 cut(s) 62, 215
Fsp4HI GCNGC 2 cut(s) 114, 233
GluI GCNGC 2 cut(s) 114, 233
HapII CCGG 2 cut(s) 347, 444
Hin1II CATG 2 cut(s) 548, 580
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HpaII CCGG 2 cut(s) 347, 444
Hpy166II GTNNAC 4 cut(s) 323, 378, 456, 490
Hpy188III TCNNGA 3 cut(s) 275, 338, 617
Hpy8I GTNNAC 4 cut(s) 323, 378, 456, 490
HpyAV CCTTC 7 cut(s) 59, 187, 220, 383, 427, 579, 667
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 2 cut(s) 171, 602
HpyF3I CTNAG 2 cut(s) 117, 525
Hsp92II CATG 2 cut(s) 548, 580
KflI GGGWCCC 1 cut(s) 161
Kzo9I GATC 3 cut(s) 590, 605, 645
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 20, 635
MalI GATC 3 cut(s) 592, 607, 647
MboI GATC 3 cut(s) 590, 605, 645
MboII GAAGA 4 cut(s) 28, 140, 146, 569
MluCI AATT 2 cut(s) 150, 649
MmeI TCCRAC 1 cut(s) 244
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 2 cut(s) 347, 444
MspR9I CCNGG 3 cut(s) 47, 318, 348
MvaI CCWGG 2 cut(s) 47, 318
NciI CCSGG 1 cut(s) 348
NdeII GATC 3 cut(s) 590, 605, 645
NlaIII CATG 2 cut(s) 548, 580
NlaIV GGNNCC 2 cut(s) 162, 163
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 2 cut(s) 548, 580
NspV TTCGAA 1 cut(s) 667
PciI ACATGT 1 cut(s) 544
PfoI TCCNGGA 1 cut(s) 346
PkrI GCNGC 2 cut(s) 115, 234
PpuMI RGGWCCY 1 cut(s) 161
PscI ACATGT 1 cut(s) 544
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 45, 316
PspGI CCWGG 2 cut(s) 45, 316
PspN4I GGNNCC 2 cut(s) 162, 163
PspPI GGNCC 1 cut(s) 161
PspPPI RGGWCCY 1 cut(s) 161
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 545
SalI GTCGAC 1 cut(s) 488
SatI GCNGC 2 cut(s) 114, 233
Sau3AI GATC 3 cut(s) 590, 605, 645
Sau96I GGNCC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 47, 318, 348
SetI ASST 9 cut(s) 224, 237, 303, 328, 394, 408, 489, 571, 659
SfaNI GCATC 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 667
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 150, 649
SsiI CCGC 2 cut(s) 113, 398
StyD4I CCNGG 3 cut(s) 45, 316, 346
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 4 cut(s) 489, 608, 660, 667
TasI AATT 2 cut(s) 150, 649
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 116
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 78, 283
TspRI CASTG 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 1 cut(s) 150
XceI RCATGY 2 cut(s) 548, 580
XmiI GTMKAC 2 cut(s) 322, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.