MD05G1357900.v1.1

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003575
Physical Location & Seq
Reverse (-)
47339339 .. 47341951
2613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1357900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGTCTCTGGCCGTTGGAGTTGTTTCGAGTTCCAAAAACATGGGGCTGGATGATGAAAACACCGCCGAGGTCTCCAACAAGACGGCGAAAACTGGTGGGGCAAATGTGGATCAACCGTCCACAGGGTTGGTTAGAGAACCCAGCGAGGGTTCCCTCTATCAGACCGAGGACGAGGACGACGATGACGAAGTAAAGAAGATTGAGTTGGGGCCTCAGTGCACTCTCAAAGAACAAATTGAGAAGGACGCGGACGATGAGAGCTTGAGGAGGTGGAAGGAACAGCTTCTTGGGAGTGTAGATGTTAATTCTGTTGGAGAAACTCTAGAACCAGATGTTAAGATCATGAGCCTTGCAATTAAATCCCCTGGCAGATCTGATATAATTCTTCCGATTCCTGAGAACGGAAATCCCAAAGGGTTGTGGTTTACTTTAAAAGAAGGTAGCCGTTACAGCCTCGAATTCACTATTCAGGTCAGCAATAACATTGTTTCAGGTCTCAAATACACAAACACAGTCTGGAAAACTGCTGTGAAAGTTGATAGCACAAGAGAGATGCTTGGAACATTTAGTCCACAGTCAGAACCTTACACACATGTGTTGCCTGAGGACACAACCCCATCTGGCATATTTGCTAGGGGATCTTATTCAGCTCGAAGCAAGTTTGTTGACGATGACAACAAGTGCTACTTGGAAATCAATTACACCTTTGATATCCGAAAAGACTGGCAATCGGCGTAA

Protein Analysis

246

Amino Acids

27.11

Weight (kDa)

4.57

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 55 - 241 9.9e-85 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 248
AciI CCGC 2 cut(s) 63, 248
AclWI GGATC 2 cut(s) 117, 646
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 458
AfiI CCNNNNNNNGG 3 cut(s) 122, 146, 401
AflIII ACRYGT 1 cut(s) 592
AjnI CCWGG 1 cut(s) 364
AjuI GAANNNNNNNTTGG 4 cut(s) 188, 220, 270, 302
AleI CACNNNNGTG 1 cut(s) 593
AloI GAACNNNNNNTCC 2 cut(s) 553, 585
AluBI AGCT 3 cut(s) 261, 283, 650
AluI AGCT 3 cut(s) 261, 283, 650
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 3 cut(s) 9, 76, 500
Alw44I GTGCAC 1 cut(s) 217
AlwI GGATC 2 cut(s) 117, 646
AoxI GGCC 2 cut(s) 9, 209
ApaLI GTGCAC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 209
AxyI CCTNAGG 1 cut(s) 603
BaeGI GKGCMC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 221
BccI CCATC 1 cut(s) 625
BceAI ACGGC 2 cut(s) 99, 429
BciT130I CCWGG 1 cut(s) 366
BcoDI GTCTC 3 cut(s) 9, 76, 500
BfaI CTAG 2 cut(s) 323, 633
BglII AGATCT 1 cut(s) 371
Bme1390I CCNGG 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 151, 210
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 283
BsaI GGTCTC 2 cut(s) 76, 500
BsaJI CCNNGG 3 cut(s) 66, 165, 364
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 146, 401
Bse1I ACTGG 2 cut(s) 97, 728
Bse21I CCTNAGG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 3 cut(s) 66, 165, 364
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 3 cut(s) 122, 146, 401
BseMII CTCAG 3 cut(s) 227, 387, 594
BseNI ACTGG 2 cut(s) 97, 728
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 2 cut(s) 11, 211
BsiHKAI GWGCWC 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 122, 146, 401
BsmAI GTCTC 3 cut(s) 9, 76, 500
BsnI GGCC 2 cut(s) 11, 211
Bso31I GGTCTC 2 cut(s) 76, 500
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 4 cut(s) 109, 339, 371, 638
BspACI CCGC 2 cut(s) 63, 248
BspANI GGCC 2 cut(s) 11, 211
BspCNI CTCAG 3 cut(s) 226, 388, 595
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 151, 210
BspPI GGATC 2 cut(s) 117, 646
BspTNI GGTCTC 2 cut(s) 76, 500
BsrI ACTGG 2 cut(s) 97, 728
BssECI CCNNGG 3 cut(s) 66, 165, 364
BssMI GATC 4 cut(s) 109, 339, 371, 638
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 3 cut(s) 117, 514, 576
BstDEI CTNAG 3 cut(s) 213, 396, 603
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 248
BstKTI GATC 4 cut(s) 112, 342, 374, 641
BstMAI GTCTC 3 cut(s) 9, 76, 500
BstMBI GATC 4 cut(s) 109, 339, 371, 638
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 364
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 1 cut(s) 248
BstX2I RGATCY 2 cut(s) 371, 638
BstXI CCANNNNNNTGG 2 cut(s) 40, 127
BstYI RGATCY 2 cut(s) 371, 638
Bsu36I CCTNAGG 1 cut(s) 603
BsuRI GGCC 2 cut(s) 11, 211
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 221
CciI TCATGA 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 1 cut(s) 254
CviAII CATG 3 cut(s) 40, 343, 593
CviJI RGCY 9 cut(s) 11, 46, 211, 261, 283, 348, 444, 453, 650
CviKI_1 RGCY 9 cut(s) 11, 46, 211, 261, 283, 348, 444, 453, 650
DdeI CTNAG 3 cut(s) 213, 396, 603
DpnI GATC 4 cut(s) 111, 341, 373, 640
DpnII GATC 4 cut(s) 109, 339, 371, 638
DraI TTTAAA 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 9
Eco31I GGTCTC 2 cut(s) 76, 500
Eco32I GATATC 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 364
EcoRV GATATC 1 cut(s) 712
FaeI CATG 3 cut(s) 43, 346, 596
FaiI YATR 5 cut(s) 41, 344, 380, 594, 626
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FatI CATG 3 cut(s) 39, 342, 592
FokI GGATG 1 cut(s) 62
FspBI CTAG 2 cut(s) 323, 633
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 2 cut(s) 11, 211
HgaI GACGC 1 cut(s) 254
Hin1II CATG 3 cut(s) 43, 346, 596
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 1 cut(s) 391
Hpy166II GTNNAC 5 cut(s) 120, 219, 426, 572, 667
Hpy188I TCNGA 5 cut(s) 162, 376, 390, 580, 716
Hpy188III TCNNGA 4 cut(s) 323, 343, 395, 517
Hpy8I GTNNAC 5 cut(s) 120, 219, 426, 572, 667
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 235, 268, 431
HpyCH4III ACNGT 3 cut(s) 117, 514, 576
HpyCH4V TGCA 2 cut(s) 219, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 3 cut(s) 213, 396, 603
Hsp92II CATG 3 cut(s) 43, 346, 596
Kzo9I GATC 4 cut(s) 109, 339, 371, 638
LweI GCATC 1 cut(s) 543
MaeI CTAG 2 cut(s) 323, 633
MaeIII GTNAC 1 cut(s) 446
MalI GATC 4 cut(s) 111, 341, 373, 640
MboI GATC 4 cut(s) 109, 339, 371, 638
MboII GAAGA 2 cut(s) 208, 377
MflI RGATCY 2 cut(s) 371, 638
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 6 cut(s) 234, 304, 354, 381, 458, 697
MmeI TCCRAC 2 cut(s) 99, 292
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 4 cut(s) 303, 336, 357, 431
MslI CAYNNNNRTG 1 cut(s) 593
MspR9I CCNGG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 450
NdeII GATC 4 cut(s) 109, 339, 371, 638
NlaIII CATG 3 cut(s) 43, 346, 596
NlaIV GGNNCC 2 cut(s) 151, 210
NmeAIII GCCGAG 1 cut(s) 91
NspI RCATGY 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 593
PagI TCATGA 1 cut(s) 342
PciI ACATGT 1 cut(s) 592
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 183
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 391
PscI ACATGT 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 2 cut(s) 151, 210
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 2 cut(s) 371, 638
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 4 cut(s) 303, 336, 357, 431
Sau3AI GATC 4 cut(s) 109, 339, 371, 638
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 1 cut(s) 221
SfaNI GCATC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
Sse9I AATT 6 cut(s) 234, 304, 354, 381, 458, 697
SsiI CCGC 2 cut(s) 63, 248
SspMI CTAG 2 cut(s) 323, 633
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 3 cut(s) 117, 514, 576
TaqI TCGA 3 cut(s) 26, 456, 652
TaqII GACCGA 1 cut(s) 179
TasI AATT 6 cut(s) 234, 304, 354, 381, 458, 697
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 303, 336, 357, 431
Tru9I TTAA 4 cut(s) 303, 336, 357, 431
TscAI CASTG 1 cut(s) 221
TspDTI ATGAA 1 cut(s) 69
TspGWI ACGGA 1 cut(s) 417
TspRI CASTG 1 cut(s) 221
VneI GTGCAC 1 cut(s) 217
XapI RAATTY 1 cut(s) 458
XbaI TCTAGA 1 cut(s) 322
XceI RCATGY 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 2 cut(s) 323, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.