Rw2G043310

Rho GDP-dissociation inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003575
Physical Location & Seq
Forward (+)
69704917 .. 69707213
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043310.1

Sequence Viewer

Length: 690 bp
ATGGGTGGAGGACAGAAGAGTGACAAAGAAAAAGTGGAGGAAGAAGATACTGATAATGAAGCAGGAGAGGGACTTCCCAGGCAGATGAGTGAGACTTCTGTTTCTGCAGCTGAGGATGATGATGATGAAGAAAACAGCAACAAGATTCAGTTGGGTCCTCAATGCACTCTCAAAGAACAGATTGAGAAGGATAAGCTGTGTGATGATGAGAGCTTGAGGAGGTGGAAGGAACAGCTTCTTGGGGCTGTGGATTTTGAGAGTGTTGGAGAAACTCTAGACCCAGAAGTAAAGATTATTAGCCTCTCAATCCTCACTCCTGATAGACCTGACATTGTTCTTGATGTTCCGGACAATGGAAAACCCAAAGGCTTGTGGTTTACTCTGAAGGAAGGTAGTCCTCACAACTTGAAATTCTCCTTCCGAGTTAGCAATAACATTGTATCTGGCCTCAAGTACACCAATACTGTTTGGAAAACCGGCGTCAAGGTGGACAGCACAAAAGAGATGATTGGAACCTTCAGTCCTCAGCCAGAGCCATACACACATGTGATGCCAGAAGTGGTCACACCTTCCGGCATGTTTGCAAGAGGATCATATTCTGCAAGATCAAAGTTTCTTGACGATGATAACAAGTGCTACTTGGAAATCAATTACACCTTTGATATTCGAAAGGAATGGGCTGCGTGCTAG

Protein Analysis

229

Amino Acids

25.69

Weight (kDa)

4.61

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_GDI PF02115 37 - 225 2.4e-83 RHO protein GDP dissociation inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 346
AclWI GGATC 1 cut(s) 598
AcsI RAATTY 1 cut(s) 410
AcuI CTGAAG 2 cut(s) 404, 502
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 1 cut(s) 409
AjnI CCWGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AleI CACNNNNGTG 1 cut(s) 545
AloI GAACNNNNNNTCC 2 cut(s) 505, 537
AluBI AGCT 4 cut(s) 110, 196, 213, 235
AluI AGCT 4 cut(s) 110, 196, 213, 235
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 598
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 2 cut(s) 107, 680
ApoI RAATTY 1 cut(s) 410
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 155
AsuII TTCGAA 1 cut(s) 667
AvaII GGWCC 1 cut(s) 155
BbvCI CCTCAGC 2 cut(s) 111, 525
BbvI GCAGC 2 cut(s) 119, 667
BciT130I CCWGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 275, 688
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 2 cut(s) 108, 681
BlsI GCNGC 2 cut(s) 109, 682
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 2 cut(s) 156, 514
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 540
Bpu10I CCTNAGC 2 cut(s) 111, 525
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 2 cut(s) 235, 434
BsaHI GRCGYC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse118I RCCGGY 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 102, 539
BseRI GAGGAG 1 cut(s) 232
BseXI GCAGC 2 cut(s) 119, 667
BshFI GGCC 1 cut(s) 447
BsiSI CCGG 3 cut(s) 347, 477, 573
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 84
BsnI GGCC 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 667
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 2 cut(s) 590, 605
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 2 cut(s) 103, 538
BspEI TCCGGA 1 cut(s) 346
BspLI GGNNCC 2 cut(s) 156, 514
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 598
BspT104I TTCGAA 1 cut(s) 667
BsrFI RCCGGY 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 2 cut(s) 590, 605
BssNI GRCGYC 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 667
BstC8I GCNNGC 1 cut(s) 685
BstDEI CTNAG 2 cut(s) 111, 525
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 2 cut(s) 593, 608
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 590, 605
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 2 cut(s) 548, 580
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 119, 667
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 1 cut(s) 469
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 545, 577
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 2 cut(s) 111, 525
DpnI GATC 2 cut(s) 592, 607
DpnII GATC 2 cut(s) 590, 605
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 404, 502
EcoO109I RGGNCCY 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 77
FaeI CATG 2 cut(s) 548, 580
FaiI YATR 4 cut(s) 538, 546, 578, 595
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 544, 576
Fnu4HI GCNGC 2 cut(s) 108, 681
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 108, 681
FspBI CTAG 2 cut(s) 275, 688
GluI GCNGC 2 cut(s) 108, 681
HaeIII GGCC 1 cut(s) 447
HapII CCGG 3 cut(s) 347, 477, 573
HgaI GACGC 1 cut(s) 469
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 2 cut(s) 548, 580
HinfI GANTC 1 cut(s) 145
HpaII CCGG 3 cut(s) 347, 477, 573
Hpy166II GTNNAC 3 cut(s) 378, 456, 490
Hpy188I TCNGA 2 cut(s) 384, 422
Hpy188III TCNNGA 5 cut(s) 275, 317, 338, 347, 617
Hpy8I GTNNAC 3 cut(s) 378, 456, 490
HpyAV CCTTC 7 cut(s) 181, 220, 379, 383, 427, 526, 579
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 4 cut(s) 107, 165, 584, 602
HpyF3I CTNAG 2 cut(s) 111, 525
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 2 cut(s) 548, 580
Kpn2I TCCGGA 1 cut(s) 346
Kzo9I GATC 2 cut(s) 590, 605
Lsp1109I GCAGC 2 cut(s) 119, 667
LweI GCATC 1 cut(s) 540
MaeI CTAG 2 cut(s) 275, 688
MaeIII GTNAC 2 cut(s) 20, 562
MalI GATC 2 cut(s) 592, 607
MboI GATC 2 cut(s) 590, 605
MboII GAAGA 4 cut(s) 28, 53, 56, 140
MluCI AATT 2 cut(s) 410, 649
MmeI TCCRAC 1 cut(s) 244
MroI TCCGGA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 347, 477, 573
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
NdeII GATC 2 cut(s) 590, 605
NlaIII CATG 2 cut(s) 548, 580
NlaIV GGNNCC 2 cut(s) 156, 514
NmuCI GTSAC 2 cut(s) 20, 562
NspI RCATGY 2 cut(s) 548, 580
NspV TTCGAA 1 cut(s) 667
OliI CACNNNNGTG 1 cut(s) 545
PciI ACATGT 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 1 cut(s) 145
PkrI GCNGC 2 cut(s) 109, 682
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 544
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 156, 514
PspPI GGNCC 1 cut(s) 155
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 545
SatI GCNGC 2 cut(s) 108, 681
Sau3AI GATC 2 cut(s) 590, 605
Sau96I GGNCC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 79
SfaNI GCATC 1 cut(s) 540
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 667
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 2 cut(s) 214, 449
SmoI CTYRAG 2 cut(s) 214, 449
Sse9I AATT 2 cut(s) 410, 649
SspMI CTAG 2 cut(s) 275, 688
StyD4I CCNGG 1 cut(s) 77
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 667
TasI AATT 2 cut(s) 410, 649
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 1 cut(s) 145
TseFI GTSAC 2 cut(s) 20, 562
TseI GCWGC 2 cut(s) 107, 680
Tsp45I GTSAC 2 cut(s) 20, 562
TspDTI ATGAA 2 cut(s) 72, 141
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 1 cut(s) 410
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 2 cut(s) 548, 580
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 2 cut(s) 275, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.