Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002971

Physical Location & Seq

Genomic Coordinates Forward (+)

37868461 .. 37874992

Gene Structure 6532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g50990.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGGCTGCCACTGCAACTGCCTCTGCTGGTCCTCGATATGCTCCAGAGGATCCAACTCTCCCAAAGCCTTGGAAAGGATTGGTTGATGGTAAAACTGGGTATCTCTACTTCTGGAACCCTGAGACCAATGTGACCCAATATGATAGGCCTACGGGCTCAGCTCCTCCACCAAAGGCTCCTTCTGCGCAGTATATAAGTTCTTCAGTTCAAGTTCAACAATCTTCACAAGGACAACGTCGTGATGAGGGAGATGATCGGTATGGCAGGGGCAGCAATGGTAGTTCAAAATTTGAGGCAGCACCGAGGAATGTGCACGTAGTTGAGACTGCAAGAAGTGCACCAGTTTCTTCTAACAATGGTCCAAGTGGGCCAGCTAATGCACATGGTGGATCTCTTATTAGAGGAAATGGACCATCAGACACTGGAAGTGGTTTGTCTGCTGAGGCTTATTGTCGCCGGCATGAAATTACTGCTATTGGTGATAATGTGCCCCCACCATTTACTACCTTCGAAGCTACGGGCTTCCCATCGGAGATTCTTAGAGAGGTACAAAACGCTGGATTTTCTGCCCCGACTCCAATTCAAGCTCAGTCATGGCCGGTTGCTCTTCAAAGTAGAGATATTGTGGCCATTGCTAAAACGGGATCAGGGAAGACCTTGGGTTACTTGCTTCCTGGATTTATGCATCTCAAGCGCACCCGTAATAATTCTCAGATGGGTCCAACTGTGTTGGTGTTATCACCAACAAGGGAGCTGGCCACACAGATTCAAGATGAAGCAATGAAGTTTGGGAAATCTTCAAGAATAGCTGTTGTGTGTTTGTATGGAGGAGCGCCAAAGGGTCCACAATTGAGAGATATTGACAGAGGAGCAGATGTTGTGGTTGCCACTCCTGGTCGCTTAAATGACATTCTAGAAATGAAGAGAATTAGTCTCCATCAAGTCTCTTACCTAGTGTTGGATGAGGCTGATCGTATGCTGGATATGGGTTTTGAACCTCAAATAAGGAAGATTGTGAAGGAGATACCTGCTCGCCGTCAGACATTGATGTACACAGCAACATGGCCCAAGGAGGTTAGGAAGATTGCATCAGATCTACTGGTCAACTCTGTTCAGGTTAACATTGGCAATGTTGATGAACTTGTCGCGAATAAGTCGATCACACAGCATGTTGAAGTGATGTCGTCAATGGAGAAACACAGACGGTTGGAACAGATATTGCGATCGCAAGAGCCAGGGTCCAAGATAATTATATTTTGCTCAACCAAAAAGATGTGTGATCAACTTTCTCGCAACCTAACCCGCCAATTTGGAGCTGCTGCAATTCATGGTGACAAATCTCAGGCTGAGAGGGATCATGTCCTGAGTCAATTTCGCACAGGGAGGACTCCAATTCTTGTAGCAACTGATGTTGCAGCTCGAGGATTGGATGTTAAGGACATAAGGGTGGTTATCAATTACGACTTCCCTACTGGAGTAGAGGATTATGTTCACAGGATTGGGAGAACTGGAAGGGCAGGTGCCACTGGGTTGGCATACACATTCTTTGGTGATCAGGATGCAAAATATGCTGCAGAACTCATTAAGGTTTTGGAAGGAGCTAACCAACGTGTACCCCCAGAAATTCGTGATTTGGCTTCACGTGGTGGTGGGATGAATAGGTTCAGACGTTGGGGCTCTGGATCTGGTGGTCGTGATGGTGGACGAAGTGATTCAAGCTATGGTGGAAGAGATGGTGGAAGGGGCGGTGGGTTTGGATCTTCTTTCTCTGCTGGCAGATATGACCGAGGTCCAAATAATAGTTATGATGGCAAAGGTTTGACTCGGGAGCGTAGTCGTAGTCCTGAAAGGTTCAACAAAGCTCCGCCAACAGCTGTTGGCTCTGGAGGTTGGAGTTTTCACAAAGCAATGATGGAACAACGCGGACGGTCATCACCGCCACCTCCAAATCGTGTTAAGGGAGTAGTGGATGGTTTCCGTCCTTCTTTCTATGGACTGGAAGAAGCTGAGGAAGGCATGATACGTCAAGACGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009628 GO:0009651 GO:0009987 GO:0010501 GO:0016070 GO:0016301 GO:0016310 GO:0016462 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031974 GO:0031981 GO:0034641 GO:0036211 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046777 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140096 GO:0140098 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

679

Amino Acids

73.61

Weight (kDa)

9.55

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	20 - 50	6.2e-07	WW domain
DEAD	PF00270	192 - 361	4.9e-49	DEAD/DEAH box helicase
ResIII	PF04851	204 - 355	3.2e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	399 - 507	4.3e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01540 AT3G01540 AT3G01540 AT3G01540 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610
fragaria_vesca	FvH4_3g33550 FvH4_6g50990
malus_domestica	MD09G1029800.v1.1 MD17G1031800.v1.1
prunus_persica	Prupe.3G287500_v2.0.a1 Prupe.3G287500_v2.0.a1
pyrus_communis	pycom17g02940
rosa_chinensis	RchiOBHm_Chr2g0172491 RchiOBHm_Chr3g0496011
rosa_laevigata	RLG00000022110 RLG00000026510
rosa_multiflora	Rmu_co8447803.1_g000001 Rmu_sc0008577.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079810 Rroxscaffold_6G00401610
rosa_rugosa	Rorug02G0559500
rosa_samantha	Rh1CG060200 Rh2AG635200 Rh2BG648700 Rh2CG615300 Rh2DG663200 Rh3AG325700 Rh5DG348900 Rh5DG552200 Rh7AG279200 Rh7CG298300
rosa_wichuraiana	Rw2G052550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1508
Acc16I	TGCGCA	1 cut(s)	186
Acc36I	ACCTGC	2 cut(s)	1036, 1508
AccB1I	GGYRCC	1 cut(s)	1520
AccII	CGCG	2 cut(s)	1148, 1923
AciI	CCGC	5 cut(s)	1303, 1746, 1865, 1923, 1937
AclWI	GGATC	7 cut(s)	44, 57, 397, 652, 1362, 1690, 1765
AcoI	YGGCCR	3 cut(s)	596, 627, 756
AcsI	RAATTY	2 cut(s)	287, 1623
AcuI	CTGAAG	1 cut(s)	186
AcvI	CACGTG	1 cut(s)	1643
AdeI	CACNNNGTG	2 cut(s)	386, 1646
AfaI	GTAC	3 cut(s)	549, 1052, 1614
AfiI	CCNNNNNNNGG	2 cut(s)	74, 958
AflIII	ACRYGT	1 cut(s)	1609
AjnI	CCWGG	3 cut(s)	673, 892, 1234
AjuI	GAANNNNNNNTTGG	2 cut(s)	163, 195
Alw21I	GWGCWC	2 cut(s)	315, 340
Alw26I	GTCTC	4 cut(s)	116, 317, 938, 949
Alw44I	GTGCAC	2 cut(s)	311, 336
AlwI	GGATC	7 cut(s)	44, 57, 397, 652, 1362, 1690, 1765
AlwNI	CAGNNNCTG	1 cut(s)	422
Ama87I	CYCGRG	2 cut(s)	1419, 1824
AoxI	GGCC	6 cut(s)	146, 368, 596, 627, 756, 1064
ApaLI	GTGCAC	2 cut(s)	311, 336
ApeKI	GCWGC	7 cut(s)	5, 270, 296, 1316, 1319, 1415, 1571
ApoI	RAATTY	2 cut(s)	287, 1623
AsiSI	GCGATCGC	1 cut(s)	1226
Asp700I	GAANNNNTTC	2 cut(s)	1661, 1711
AspLEI	GCGC	3 cut(s)	187, 696, 835
AspS9I	GGNCC	9 cut(s)	29, 359, 368, 410, 719, 842, 1065, 1239, 1790
AsuHPI	GGTGA	5 cut(s)	491, 732, 1343, 1562, 1926
AsuII	TTCGAA	1 cut(s)	510
AvaI	CYCGRG	2 cut(s)	1419, 1824
AvaII	GGWCC	7 cut(s)	29, 359, 410, 719, 842, 1239, 1790
BaeGI	GKGCMC	3 cut(s)	315, 340, 492
BalI	TGGCCA	2 cut(s)	629, 758
BamHI	GGATCC	1 cut(s)	49
BanI	GGYRCC	1 cut(s)	1520
BanII	GRGCYC	2 cut(s)	158, 1679
BarI	GAAGNNNNNNTAC	4 cut(s)	92, 124, 2004, 2036
BbrPI	CACGTG	1 cut(s)	1643
BbsI	GAAGAC	1 cut(s)	659
Bbv12I	GWGCWC	2 cut(s)	315, 340
BbvCI	CCTCAGC	2 cut(s)	441, 2007
BbvI	GCAGC	6 cut(s)	282, 308, 1303, 1306, 1427, 1558
BceAI	ACGGC	1 cut(s)	1020
BcgI	CGANNNNNNTGC	2 cut(s)	292, 326
BciT130I	CCWGG	3 cut(s)	675, 894, 1236
BclI	TGATCA	2 cut(s)	1279, 1552
BcoDI	GTCTC	4 cut(s)	116, 317, 938, 949
BfaI	CTAG	2 cut(s)	914, 953
BfmI	CTRYAG	1 cut(s)	1572
BfoI	RGCGCY	1 cut(s)	836
BfuAI	ACCTGC	2 cut(s)	1036, 1508
BglII	AGATCT	1 cut(s)	1093
BisI	GCNGC	7 cut(s)	6, 271, 297, 1317, 1320, 1416, 1572
BlpI	GCTNAGC	1 cut(s)	157
BlsI	GCNGC	7 cut(s)	7, 272, 298, 1318, 1321, 1417, 1573
Bme1390I	CCNGG	3 cut(s)	675, 894, 1236
Bme18I	GGWCC	7 cut(s)	29, 359, 410, 719, 842, 1239, 1790
BmeT110I	CYCGRG	2 cut(s)	1419, 1824
BmgT120I	GGNCC	9 cut(s)	29, 359, 368, 410, 719, 842, 1065, 1239, 1790
BmiI	GGNNCC	7 cut(s)	51, 116, 177, 720, 843, 1240, 1522
BmrFI	CCNGG	3 cut(s)	675, 894, 1236
BmrI	ACTGGG	2 cut(s)	105, 1536
BmsI	GCATC	3 cut(s)	694, 1097, 1549
BmuI	ACTGGG	2 cut(s)	105, 1536
BoxI	GACNNNNGTC	1 cut(s)	1788
BpiI	GAAGAC	1 cut(s)	659
BpmI	CTGGAG	3 cut(s)	27, 1494, 1905
Bpu10I	CCTNAGC	2 cut(s)	441, 2007
Bpu1102I	GCTNAGC	1 cut(s)	157
Bpu14I	TTCGAA	1 cut(s)	510
BpuEI	CTTGAG	1 cut(s)	674
BsaAI	YACGTR	2 cut(s)	316, 1643
BsaI	GGTCTC	1 cut(s)	116
BsaJI	CCNNGG	6 cut(s)	68, 302, 657, 1068, 1235, 1786
BsaXI	ACNNNNNCTCC	2 cut(s)	861, 891
Bsc4I	CCNNNNNNNGG	2 cut(s)	74, 958
Bse118I	RCCGGY	2 cut(s)	456, 598
Bse1I	ACTGG	8 cut(s)	100, 341, 427, 1104, 1477, 1513, 1531, 2001
Bse3DI	GCAATG	5 cut(s)	280, 630, 786, 1135, 1914
BseBI	CCWGG	3 cut(s)	675, 894, 1236
BseDI	CCNNGG	6 cut(s)	68, 302, 657, 1068, 1235, 1786
BseGI	GGATG	5 cut(s)	967, 1435, 1564, 1659, 1975
BseLI	CCNNNNNNNGG	2 cut(s)	74, 958
BseMI	GCAATG	5 cut(s)	280, 630, 786, 1135, 1914
BseMII	CTCAG	9 cut(s)	111, 171, 432, 602, 725, 1338, 1355, 1355, 1998
BseNI	ACTGG	8 cut(s)	100, 341, 427, 1104, 1477, 1513, 1531, 2001
BseRI	GAGGAG	3 cut(s)	153, 843, 882
BseSI	GKGCMC	3 cut(s)	315, 340, 492
BseXI	GCAGC	6 cut(s)	282, 308, 1303, 1306, 1427, 1558
Bsh1236I	CGCG	2 cut(s)	1148, 1923
Bsh1285I	CGRYCG	1 cut(s)	1226
BshFI	GGCC	6 cut(s)	148, 370, 598, 629, 758, 1066
BshNI	GGYRCC	1 cut(s)	1520
BsiEI	CGRYCG	1 cut(s)	1226
BsiHKAI	GWGCWC	2 cut(s)	315, 340
BsiHKCI	CYCGRG	2 cut(s)	1419, 1824
BsiSI	CCGG	2 cut(s)	457, 599
BslI	CCNNNNNNNGG	2 cut(s)	74, 958
BsmAI	GTCTC	4 cut(s)	116, 317, 938, 949
BsnI	GGCC	6 cut(s)	148, 370, 598, 629, 758, 1066
Bso31I	GGTCTC	1 cut(s)	116
BsoBI	CYCGRG	2 cut(s)	1419, 1824
Bsp119I	TTCGAA	1 cut(s)	510
Bsp1286I	GDGCHC	5 cut(s)	158, 315, 340, 492, 1679
Bsp1407I	TGTACA	1 cut(s)	1050
Bsp1720I	GCTNAGC	1 cut(s)	157
Bsp68I	TCGCGA	1 cut(s)	1148
BspACI	CCGC	5 cut(s)	1303, 1746, 1865, 1923, 1937
BspANI	GGCC	6 cut(s)	148, 370, 598, 629, 758, 1066
BspCNI	CTCAG	9 cut(s)	112, 170, 433, 601, 724, 1339, 1354, 1356, 1999
BspFNI	CGCG	2 cut(s)	1148, 1923
BspLI	GGNNCC	7 cut(s)	51, 116, 177, 720, 843, 1240, 1522
BspMAI	CTGCAG	1 cut(s)	1576
BspMI	ACCTGC	2 cut(s)	1036, 1508
BspPI	GGATC	7 cut(s)	44, 57, 397, 652, 1362, 1690, 1765
BspQI	GCTCTTC	1 cut(s)	612
BspT104I	TTCGAA	1 cut(s)	510
BspT107I	GGYRCC	1 cut(s)	1520
BspTNI	GGTCTC	1 cut(s)	116
BsrDI	GCAATG	5 cut(s)	280, 630, 786, 1135, 1914
BsrFI	RCCGGY	2 cut(s)	456, 598
BsrGI	TGTACA	1 cut(s)	1050
BsrI	ACTGG	8 cut(s)	100, 341, 427, 1104, 1477, 1513, 1531, 2001
BssAI	RCCGGY	2 cut(s)	456, 598
BssECI	CCNNGG	6 cut(s)	68, 302, 657, 1068, 1235, 1786
BssT1I	CCWWGG	3 cut(s)	68, 657, 1068
Bst2UI	CCWGG	3 cut(s)	675, 894, 1236
Bst4CI	ACNGT	3 cut(s)	727, 1206, 1929
Bst6I	CTCTTC	3 cut(s)	612, 917, 1723
BstAPI	GCANNNNNTGC	2 cut(s)	335, 1568
BstAUI	TGTACA	1 cut(s)	1050
BstBAI	YACGTR	2 cut(s)	316, 1643
BstBI	TTCGAA	1 cut(s)	510
BstC8I	GCNNGC	5 cut(s)	372, 458, 756, 1033, 1774
BstENI	CCTNNNNNAGG	1 cut(s)	72
BstF5I	GGATG	5 cut(s)	967, 1435, 1564, 1659, 1975
BstFNI	CGCG	2 cut(s)	1148, 1923
BstH2I	RGCGCY	1 cut(s)	836
BstHHI	GCGC	3 cut(s)	187, 696, 835
BstMAI	GTCTC	4 cut(s)	116, 317, 938, 949
BstMCI	CGRYCG	1 cut(s)	1226
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 182, 270, 335, 691, 1568
BstNI	CCWGG	3 cut(s)	675, 894, 1236
BstNSI	RCATGY	1 cut(s)	1172
BstPAI	GACNNNNGTC	1 cut(s)	1788
BstSCI	CCNGG	3 cut(s)	673, 892, 1234
BstSFI	CTRYAG	1 cut(s)	1572
BstSLI	GKGCMC	3 cut(s)	315, 340, 492
BstUI	CGCG	2 cut(s)	1148, 1923
BstV1I	GCAGC	6 cut(s)	282, 308, 1303, 1306, 1427, 1558
BstV2I	GAAGAC	1 cut(s)	659
BstX2I	RGATCY	5 cut(s)	49, 389, 1093, 1682, 1757
BstXI	CCANNNNNNTGG	2 cut(s)	69, 1531
BstYI	RGATCY	5 cut(s)	49, 389, 1093, 1682, 1757
BsuRI	GGCC	6 cut(s)	148, 370, 598, 629, 758, 1066
BtsCI	GGATG	5 cut(s)	967, 1435, 1564, 1659, 1975
BtsI	GCAGTG	1 cut(s)	9
BtsIMutI	CAGTG	3 cut(s)	9, 420, 1524
BtuMI	TCGCGA	1 cut(s)	1148
BveI	ACCTGC	2 cut(s)	1036, 1508
Cac8I	GCNNGC	5 cut(s)	372, 458, 756, 1033, 1774
CaiI	CAGNNNCTG	1 cut(s)	422
CfoI	GCGC	3 cut(s)	187, 696, 835
Cfr10I	RCCGGY	2 cut(s)	456, 598
Cfr13I	GGNCC	9 cut(s)	29, 359, 368, 410, 719, 842, 1065, 1239, 1790
Csp6I	GTAC	3 cut(s)	548, 1051, 1613
CviAII	CATG	8 cut(s)	383, 461, 594, 1062, 1169, 1328, 1358, 2017
CviQI	GTAC	3 cut(s)	548, 1051, 1613
DraIII	CACNNNGTG	2 cut(s)	386, 1646
EaeI	YGGCCR	3 cut(s)	596, 627, 756
Eam1104I	CTCTTC	3 cut(s)	612, 917, 1723
EarI	CTCTTC	3 cut(s)	612, 917, 1723
EciI	GGCGGA	1 cut(s)	1854
Eco130I	CCWWGG	3 cut(s)	68, 657, 1068
Eco147I	AGGCCT	1 cut(s)	148
Eco24I	GRGCYC	2 cut(s)	158, 1679
Eco31I	GGTCTC	1 cut(s)	116
Eco47I	GGWCC	7 cut(s)	29, 359, 410, 719, 842, 1239, 1790
Eco57I	CTGAAG	1 cut(s)	186
Eco72I	CACGTG	1 cut(s)	1643
Eco88I	CYCGRG	2 cut(s)	1419, 1824
EcoNI	CCTNNNNNAGG	1 cut(s)	72
EcoRII	CCWGG	3 cut(s)	673, 892, 1234
EcoT14I	CCWWGG	3 cut(s)	68, 657, 1068
EcoT22I	ATGCAT	1 cut(s)	687
EcoT38I	GRGCYC	2 cut(s)	158, 1679
ErhI	CCWWGG	3 cut(s)	68, 657, 1068
FaeI	CATG	8 cut(s)	386, 464, 597, 1065, 1172, 1331, 1361, 2020
FatI	CATG	8 cut(s)	382, 460, 593, 1061, 1168, 1327, 1357, 2016
FauI	CCCGC	1 cut(s)	1310
FbaI	TGATCA	2 cut(s)	1279, 1552
Fnu4HI	GCNGC	7 cut(s)	6, 271, 297, 1317, 1320, 1416, 1572
FokI	GGATG	5 cut(s)	974, 1442, 1571, 1666, 1982
FriOI	GRGCYC	2 cut(s)	158, 1679
Fsp4HI	GCNGC	7 cut(s)	6, 271, 297, 1317, 1320, 1416, 1572
FspBI	CTAG	2 cut(s)	914, 953
FspI	TGCGCA	1 cut(s)	186
GlaI	GCGC	3 cut(s)	186, 695, 834
GluI	GCNGC	7 cut(s)	6, 271, 297, 1317, 1320, 1416, 1572
GsuI	CTGGAG	3 cut(s)	27, 1494, 1905
HaeII	RGCGCY	1 cut(s)	836
HaeIII	GGCC	6 cut(s)	148, 370, 598, 629, 758, 1066
HapII	CCGG	2 cut(s)	457, 599
HhaI	GCGC	3 cut(s)	187, 696, 835
Hin1II	CATG	8 cut(s)	386, 464, 597, 1065, 1172, 1331, 1361, 2020
Hin6I	GCGC	3 cut(s)	185, 694, 833
HinP1I	GCGC	3 cut(s)	185, 694, 833
HincII	GTYRAC	2 cut(s)	1105, 1120
HindII	GTYRAC	2 cut(s)	1105, 1120
HinfI	GANTC	7 cut(s)	535, 574, 766, 1366, 1387, 1712, 1822
HpaI	GTTAAC	1 cut(s)	1120
HpaII	CCGG	2 cut(s)	457, 599
HphI	GGTGA	5 cut(s)	491, 732, 1343, 1562, 1926
Hpy166II	GTNNAC	9 cut(s)	313, 338, 845, 1053, 1105, 1120, 1492, 1613, 1703
Hpy188I	TCNGA	6 cut(s)	418, 532, 714, 1041, 1093, 1667
Hpy8I	GTNNAC	9 cut(s)	313, 338, 845, 1053, 1105, 1120, 1492, 1613, 1703
Hpy99I	CGWCG	1 cut(s)	240
HpyAV	CCTTC	8 cut(s)	189, 517, 1012, 1506, 1589, 1734, 1992, 2006
HpyCH4III	ACNGT	3 cut(s)	727, 1206, 1929
HpyCH4IV	ACGT	6 cut(s)	235, 315, 1609, 1642, 1669, 2023
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 182, 270, 335, 691, 1568
HpySE526I	ACGT	6 cut(s)	235, 315, 1609, 1642, 1669, 2023
Hsp92II	CATG	8 cut(s)	386, 464, 597, 1065, 1172, 1331, 1361, 2020
HspAI	GCGC	3 cut(s)	185, 694, 833
KroI	GCCGGC	1 cut(s)	456
KroNI	GCCGGC	1 cut(s)	458
Ksp22I	TGATCA	2 cut(s)	1279, 1552
KspAI	GTTAAC	1 cut(s)	1120
LguI	GCTCTTC	1 cut(s)	612
Lsp1109I	GCAGC	6 cut(s)	282, 308, 1303, 1306, 1427, 1558
LweI	GCATC	3 cut(s)	694, 1097, 1549
MaeI	CTAG	2 cut(s)	914, 953
MaeII	ACGT	6 cut(s)	235, 315, 1609, 1642, 1669, 2023
MaeIII	GTNAC	3 cut(s)	130, 662, 1331
MfeI	CAATTG	1 cut(s)	848
MflI	RGATCY	5 cut(s)	49, 389, 1093, 1682, 1757
MhlI	GDGCHC	5 cut(s)	158, 315, 340, 492, 1679
MlsI	TGGCCA	2 cut(s)	629, 758
MluNI	TGGCCA	2 cut(s)	629, 758
MlyI	GAGTC	4 cut(s)	568, 1375, 1381, 1816
MmeI	TCCRAC	5 cut(s)	77, 746, 939, 1188, 1871
Mox20I	TGGCCA	2 cut(s)	629, 758
Mph1103I	ATGCAT	1 cut(s)	687
MroNI	GCCGGC	1 cut(s)	456
MroXI	GAANNNNTTC	2 cut(s)	1661, 1711
MscI	TGGCCA	2 cut(s)	629, 758
MseI	TTAA	5 cut(s)	902, 1119, 1434, 1584, 1956
MslI	CAYNNNNRTG	1 cut(s)	1445
Msp20I	TGGCCA	2 cut(s)	629, 758
MspA1I	CMGCKG	1 cut(s)	1874
MspI	CCGG	2 cut(s)	457, 599
MspR9I	CCNGG	3 cut(s)	675, 894, 1236
MunI	CAATTG	1 cut(s)	848
MvaI	CCWGG	3 cut(s)	675, 894, 1236
MvnI	CGCG	2 cut(s)	1148, 1923
MwoI	GCNNNNNNNGC	6 cut(s)	11, 182, 270, 335, 691, 1568
NaeI	GCCGGC	1 cut(s)	458
NgoMIV	GCCGGC	1 cut(s)	456
NlaIII	CATG	8 cut(s)	386, 464, 597, 1065, 1172, 1331, 1361, 2020
NlaIV	GGNNCC	7 cut(s)	51, 116, 177, 720, 843, 1240, 1522
NmuCI	GTSAC	2 cut(s)	130, 1331
NruI	TCGCGA	1 cut(s)	1148
NsbI	TGCGCA	1 cut(s)	186
NsiI	ATGCAT	1 cut(s)	687
NspI	RCATGY	1 cut(s)	1172
NspV	TTCGAA	1 cut(s)	510
PaeR7I	CTCGAG	1 cut(s)	1419
PaqCI	CACCTGC	1 cut(s)	1508
PceI	AGGCCT	1 cut(s)	148
PciSI	GCTCTTC	1 cut(s)	612
PdiI	GCCGGC	1 cut(s)	458
PdmI	GAANNNNTTC	2 cut(s)	1661, 1711
PfeI	GAWTC	3 cut(s)	535, 766, 1712
PflFI	GACNNNGTC	1 cut(s)	234
PfoI	TCCNGGA	1 cut(s)	673
PkrI	GCNGC	7 cut(s)	7, 272, 298, 1318, 1321, 1417, 1573
Ple19I	CGATCG	1 cut(s)	1226
PleI	GAGTC	4 cut(s)	568, 1374, 1381, 1816
PmaCI	CACGTG	1 cut(s)	1643
PmlI	CACGTG	1 cut(s)	1643
PpsI	GAGTC	4 cut(s)	568, 1374, 1381, 1816
Ppu21I	YACGTR	2 cut(s)	316, 1643
PshAI	GACNNNNGTC	1 cut(s)	1788
Psp6I	CCWGG	3 cut(s)	673, 892, 1234
PspCI	CACGTG	1 cut(s)	1643
PspGI	CCWGG	3 cut(s)	673, 892, 1234
PspN4I	GGNNCC	7 cut(s)	51, 116, 177, 720, 843, 1240, 1522
PspPI	GGNCC	9 cut(s)	29, 359, 368, 410, 719, 842, 1065, 1239, 1790
PspXI	VCTCGAGB	1 cut(s)	1419
PstI	CTGCAG	1 cut(s)	1576
PstNI	CAGNNNCTG	1 cut(s)	422
PsuI	RGATCY	5 cut(s)	49, 389, 1093, 1682, 1757
PsyI	GACNNNGTC	1 cut(s)	234
PvuI	CGATCG	1 cut(s)	1226
PvuII	CAGCTG	1 cut(s)	1874
RgaI	GCGATCGC	1 cut(s)	1226
RruI	TCGCGA	1 cut(s)	1148
RsaI	GTAC	3 cut(s)	549, 1052, 1614
RsaNI	GTAC	3 cut(s)	548, 1051, 1613
RseI	CAYNNNNRTG	1 cut(s)	1445
SapI	GCTCTTC	1 cut(s)	612
SaqAI	TTAA	5 cut(s)	902, 1119, 1434, 1584, 1956
SatI	GCNGC	7 cut(s)	6, 271, 297, 1317, 1320, 1416, 1572
Sau96I	GGNCC	9 cut(s)	29, 359, 368, 410, 719, 842, 1065, 1239, 1790
SchI	GAGTC	4 cut(s)	568, 1375, 1381, 1816
ScrFI	CCNGG	3 cut(s)	675, 894, 1236
SduI	GDGCHC	5 cut(s)	158, 315, 340, 492, 1679
SfaAI	GCGATCGC	1 cut(s)	1226
SfaNI	GCATC	3 cut(s)	694, 1097, 1549
SfcI	CTRYAG	1 cut(s)	1572
Sfr274I	CTCGAG	1 cut(s)	1419
SfuI	TTCGAA	1 cut(s)	510
SgfI	GCGATCGC	1 cut(s)	1226
SinI	GGWCC	7 cut(s)	29, 359, 410, 719, 842, 1239, 1790
SlaI	CTCGAG	1 cut(s)	1419
SmiMI	CAYNNNNRTG	1 cut(s)	1445
SmlI	CTYRAG	2 cut(s)	689, 1419
SmoI	CTYRAG	2 cut(s)	689, 1419
SseBI	AGGCCT	1 cut(s)	148
SsiI	CCGC	5 cut(s)	1303, 1746, 1865, 1923, 1937
SspMI	CTAG	2 cut(s)	914, 953
StuI	AGGCCT	1 cut(s)	148
StyD4I	CCNGG	3 cut(s)	673, 892, 1234
StyI	CCWWGG	3 cut(s)	68, 657, 1068
TaaI	ACNGT	3 cut(s)	727, 1206, 1929
TaiI	ACGT	6 cut(s)	238, 318, 1612, 1645, 1672, 2026
TaqI	TCGA	4 cut(s)	34, 510, 1157, 1420
TaqII	GACCGA	1 cut(s)	1800
TatI	WGTACW	1 cut(s)	1050
TfiI	GAWTC	3 cut(s)	535, 766, 1712
Tru1I	TTAA	5 cut(s)	902, 1119, 1434, 1584, 1956
Tru9I	TTAA	5 cut(s)	902, 1119, 1434, 1584, 1956
TscAI	CASTG	3 cut(s)	16, 427, 1531
TseFI	GTSAC	2 cut(s)	130, 1331
TseI	GCWGC	7 cut(s)	5, 270, 296, 1316, 1319, 1415, 1571
Tsp45I	GTSAC	2 cut(s)	130, 1331
TspDTI	ATGAA	7 cut(s)	477, 789, 797, 935, 1152, 1316, 1670
TspGWI	ACGGA	1 cut(s)	1967
TspRI	CASTG	3 cut(s)	16, 427, 1531
Tth111I	GACNNNGTC	1 cut(s)	234
VneI	GTGCAC	2 cut(s)	311, 336
VpaK11BI	GGWCC	7 cut(s)	29, 359, 410, 719, 842, 1239, 1790
XagI	CCTNNNNNAGG	1 cut(s)	72
XapI	RAATTY	2 cut(s)	287, 1623
XbaI	TCTAGA	1 cut(s)	913
XceI	RCATGY	1 cut(s)	1172
XhoI	CTCGAG	1 cut(s)	1419
XmnI	GAANNNNTTC	2 cut(s)	1661, 1711
XspI	CTAG	2 cut(s)	914, 953
Zsp2I	ATGCAT	1 cut(s)	687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.