Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002971

Physical Location & Seq

Genomic Coordinates Forward (+)

80479933 .. 80485318

Gene Structure 5386 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G052550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGGGGTACAAAATGCTGGTTTTTCTGCCCCGACTCCAATTCAAGCTCAATCATGGCCGGTTGCTCTTCAAAGTAGAGACATTGTGGCCATTGCTAAAACGGGGTCAGGGAAGACCTTGGGGTACTTGCTTCCTGGATTTATGCATCTTAAGCGCGCCCGTAATAACTCTCAGATGGGTCCAACTGTGTTGGTGTTATCACCAACAAGGGAGCTGGCCACACAGATTCAAGATGAAGCAATGAAGTTTGGAAAGTCTTCAAGAATAGCTGTTGTGTGTTTGTATGGAGGAGCGCCAAAGGGTCCACAATTGAGAGATATTGACAGAGGAGCAGATGTTGTGGTTGCCACTCCTGGCCGCTTGAATGACATTCTAGAAATGAAGAGAATCAGTCTCCATCAAGTTTCTTACCTAGTGTTGGATGAGGCTGATCGTATGCTGGACATGGGTTTTGAACCTCAAATAAGGAAGATTGTGAAGGAGATACCTGCTCGCCGCCAGACCTTAATGTATACAGCAACATGGCCCAAGGAGGTTAGGAAGATTGCATCAGATCTACTGGTCAACTCTGTTCAGGTGAACATTGGCAATGTTGATGAACTTGTCGCGAATAAGTCGATCACACAGCATGTTGAAGTACTGGCGTCCATGGAGAAACACAGACGATTGGAACAGATATTGCGATCGCAAGAGCCAGGATCCAAGATAATTATATTTTGCTCAACCAAAAAGATGTGTGATCAACTTTCTCGCAACTTAACCCGCCAGTTTGGAGCTGCTGCAATTCATGGTGACAAATCTCAGACTGAGAGGGATCATGTCCTGAGTCAATTTCGCACAGGGAGGACTCCAGTTCTTGTAGCGACTGATGTTGCAGCTCGAGGATTGGATGTTAAGGACATAAGGGTGGTTATAAATTACGACTTCCCTACTGGAGTAGAGGATTATGTTCACAGGATTGGGAGAACTGGAAGGGCAGGTGCCACTGGGTTGGCTTACACATTCTTTGGTGATCAGGATGCAAAATATGCTGCAGAACTCATTAAAGTTTTGGAAGGAGCAAACCAGCGTGTACCCCCAGAAATTCGTGAGTTGGCTTCACGTGGTGGTGGGATGAACAGATTCAGACGTTGGGGCTCTGGGTCTGGAGGTCGTGATGGTGGCCGAAGTGATTCAAGCTACGGTGGAAGAGATGGTGGAAGGGGCGGTGGGTTTGGATCTTCTTTCTCTACTGGCAGATATGACCGTGGTCCAAATAATAGTTATGATGACAAGGGGTCTGCTTGGAGCAACAAAGGTTTTACTCGGGAGCGCAGTCGTAGTCCTGAAAGGTTTAACAAAGCTCCGCCAACAGCTGTTGGCTCCGGAGGTTGGAGTTTTCACAAAGCAATGATGGAACAACGGGGACGGTCATCACCGCCTCCAAATCGTGTTGAGAAGGGAGGAGTAGATGGTTTCCGTCCTTCTTTCTATGGACTGGAAGAAGCTGAGGAAGGCATGATACCACAAGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009628 GO:0009651 GO:0009987 GO:0010501 GO:0016070 GO:0016301 GO:0016310 GO:0016462 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031974 GO:0031981 GO:0034641 GO:0036211 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046777 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140096 GO:0140098 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

505

Amino Acids

55.39

Weight (kDa)

9.7

Isoelectric Point (pI)

48.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	12 - 181	2.8e-49	DEAD/DEAH box helicase
ResIII	PF04851	24 - 175	2.1e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	219 - 327	2.9e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01540 AT3G01540 AT3G01540 AT3G01540 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610
fragaria_vesca	FvH4_3g33550 FvH4_6g50990
malus_domestica	MD09G1029800.v1.1 MD17G1031800.v1.1
prunus_persica	Prupe.3G287500_v2.0.a1 Prupe.3G287500_v2.0.a1
pyrus_communis	pycom17g02940
rosa_chinensis	RchiOBHm_Chr2g0172491 RchiOBHm_Chr3g0496011
rosa_laevigata	RLG00000022110 RLG00000026510
rosa_multiflora	Rmu_co8447803.1_g000001 Rmu_sc0008577.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079810 Rroxscaffold_6G00401610
rosa_rugosa	Rorug02G0559500
rosa_samantha	Rh1CG060200 Rh2AG635200 Rh2BG648700 Rh2CG615300 Rh2DG663200 Rh3AG325700 Rh5DG348900 Rh5DG552200 Rh7AG279200 Rh7CG298300
rosa_wichuraiana	Rw2G052550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	914
AarI	CACCTGC	1 cut(s)	968
Acc36I	ACCTGC	2 cut(s)	496, 968
AccB1I	GGYRCC	1 cut(s)	980
AccI	GTMKAC	1 cut(s)	512
AccII	CGCG	2 cut(s)	156, 608
AccIII	TCCGGA	1 cut(s)	1364
AciI	CCGC	6 cut(s)	358, 496, 763, 1206, 1346, 1418
AclWI	GGATC	4 cut(s)	693, 706, 822, 1225
AcoI	YGGCCR	5 cut(s)	56, 87, 216, 355, 1162
AcsI	RAATTY	1 cut(s)	1083
AcvI	CACGTG	1 cut(s)	1103
AcyI	GRCGYC	1 cut(s)	644
AdeI	CACNNNGTG	1 cut(s)	1106
AfaI	GTAC	4 cut(s)	9, 125, 639, 1074
AfiI	CCNNNNNNNGG	2 cut(s)	418, 1371
AflII	CTTAAG	1 cut(s)	149
AgsI	TTSAA	8 cut(s)	44, 71, 230, 261, 364, 455, 635, 1176
AhdI	GACNNNNNGTC	1 cut(s)	1276
AjnI	CCWGG	3 cut(s)	133, 352, 694
AluBI	AGCT	9 cut(s)	47, 214, 269, 776, 878, 1179, 1343, 1355, 1487
AluI	AGCT	9 cut(s)	47, 214, 269, 776, 878, 1179, 1343, 1355, 1487
Alw26I	GTCTC	2 cut(s)	72, 398
AlwI	GGATC	4 cut(s)	693, 706, 822, 1225
Ama87I	CYCGRG	2 cut(s)	879, 1305
Aor13HI	TCCGGA	1 cut(s)	1364
AoxI	GGCC	6 cut(s)	56, 87, 216, 355, 524, 1162
ApeKI	GCWGC	4 cut(s)	776, 779, 875, 1031
ApoI	RAATTY	1 cut(s)	1083
ArsI	GACNNNNNNTTYG	2 cut(s)	434, 466
AsiSI	GCGATCGC	1 cut(s)	686
Asp700I	GAANNNNTTC	3 cut(s)	256, 1121, 1171
AspLEI	GCGC	4 cut(s)	156, 158, 295, 1314
AspS9I	GGNCC	4 cut(s)	179, 302, 525, 1250
AsuHPI	GGTGA	5 cut(s)	192, 589, 803, 1022, 1407
AvaI	CYCGRG	2 cut(s)	879, 1305
AvaII	GGWCC	3 cut(s)	179, 302, 1250
BalI	TGGCCA	2 cut(s)	89, 218
BamHI	GGATCC	1 cut(s)	698
BanI	GGYRCC	1 cut(s)	980
BanII	GRGCYC	1 cut(s)	1139
BarI	GAAGNNNNNNTAC	2 cut(s)	1485, 1517
BbrPI	CACGTG	1 cut(s)	1103
BbsI	GAAGAC	2 cut(s)	119, 249
BbvCI	CCTCAGC	1 cut(s)	1488
BbvI	GCAGC	4 cut(s)	763, 766, 887, 1018
BccI	CCATC	6 cut(s)	169, 405, 1151, 1187, 1387, 1445
BciT130I	CCWGG	3 cut(s)	135, 354, 696
BclI	TGATCA	2 cut(s)	739, 1012
BcoDI	GTCTC	2 cut(s)	72, 398
BfaI	CTAG	2 cut(s)	374, 413
BfmI	CTRYAG	1 cut(s)	1032
BfoI	RGCGCY	1 cut(s)	296
BfrI	CTTAAG	1 cut(s)	149
BfuAI	ACCTGC	2 cut(s)	496, 968
BglII	AGATCT	1 cut(s)	553
BisI	GCNGC	6 cut(s)	358, 496, 777, 780, 876, 1032
BlsI	GCNGC	6 cut(s)	359, 497, 778, 781, 877, 1033
BmcAI	AGTACT	1 cut(s)	639
Bme1390I	CCNGG	3 cut(s)	135, 354, 696
Bme18I	GGWCC	3 cut(s)	179, 302, 1250
BmeRI	GACNNNNNGTC	1 cut(s)	1276
BmeT110I	CYCGRG	2 cut(s)	879, 1305
BmgT120I	GGNCC	4 cut(s)	179, 302, 525, 1250
BmiI	GGNNCC	5 cut(s)	180, 303, 700, 982, 1363
BmrFI	CCNGG	3 cut(s)	135, 354, 696
BmrI	ACTGGG	1 cut(s)	996
BmsI	GCATC	3 cut(s)	154, 557, 1009
BmuI	ACTGGG	1 cut(s)	996
BoxI	GACNNNNGTC	1 cut(s)	1248
BpiI	GAAGAC	2 cut(s)	119, 249
BpmI	CTGGAG	3 cut(s)	834, 954, 1167
Bpu10I	CCTNAGC	1 cut(s)	1488
BsaAI	YACGTR	1 cut(s)	1103
BsaHI	GRCGYC	1 cut(s)	644
BsaJI	CCNNGG	4 cut(s)	117, 528, 648, 1246
BsaWI	WCCGGW	1 cut(s)	1364
BsaXI	ACNNNNNCTCC	2 cut(s)	321, 351
Bsc4I	CCNNNNNNNGG	2 cut(s)	418, 1371
Bse118I	RCCGGY	1 cut(s)	58
Bse1I	ACTGG	9 cut(s)	564, 645, 766, 851, 937, 973, 991, 1237, 1482
Bse3DI	GCAATG	4 cut(s)	90, 246, 595, 1395
BseAI	TCCGGA	1 cut(s)	1364
BseBI	CCWGG	3 cut(s)	135, 354, 696
BseDI	CCNNGG	4 cut(s)	117, 528, 648, 1246
BseGI	GGATG	4 cut(s)	427, 895, 1024, 1119
BseLI	CCNNNNNNNGG	2 cut(s)	418, 1371
BseMI	GCAATG	4 cut(s)	90, 246, 595, 1395
BseMII	CTCAG	5 cut(s)	185, 798, 815, 815, 1479
BseNI	ACTGG	9 cut(s)	564, 645, 766, 851, 937, 973, 991, 1237, 1482
BsePI	GCGCGC	1 cut(s)	154
BseRI	GAGGAG	3 cut(s)	303, 342, 1458
BseXI	GCAGC	4 cut(s)	763, 766, 887, 1018
Bsh1236I	CGCG	2 cut(s)	156, 608
Bsh1285I	CGRYCG	1 cut(s)	686
BshFI	GGCC	6 cut(s)	58, 89, 218, 357, 526, 1164
BshNI	GGYRCC	1 cut(s)	980
BsiEI	CGRYCG	1 cut(s)	686
BsiHKCI	CYCGRG	2 cut(s)	879, 1305
BsiSI	CCGG	2 cut(s)	59, 1365
BslFI	GGGAC	1 cut(s)	1419
BslI	CCNNNNNNNGG	2 cut(s)	418, 1371
BsmAI	GTCTC	2 cut(s)	72, 398
BsmFI	GGGAC	1 cut(s)	1419
BsnI	GGCC	6 cut(s)	58, 89, 218, 357, 526, 1164
BsoBI	CYCGRG	2 cut(s)	879, 1305
Bsp1286I	GDGCHC	1 cut(s)	1139
Bsp13I	TCCGGA	1 cut(s)	1364
Bsp143I	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
Bsp19I	CCATGG	1 cut(s)	648
Bsp68I	TCGCGA	1 cut(s)	608
BspACI	CCGC	6 cut(s)	358, 496, 763, 1206, 1346, 1418
BspANI	GGCC	6 cut(s)	58, 89, 218, 357, 526, 1164
BspCNI	CTCAG	5 cut(s)	184, 799, 814, 816, 1480
BspEI	TCCGGA	1 cut(s)	1364
BspFNI	CGCG	2 cut(s)	156, 608
BspLI	GGNNCC	5 cut(s)	180, 303, 700, 982, 1363
BspMAI	CTGCAG	1 cut(s)	1036
BspMI	ACCTGC	2 cut(s)	496, 968
BspPI	GGATC	4 cut(s)	693, 706, 822, 1225
BspQI	GCTCTTC	1 cut(s)	72
BspT107I	GGYRCC	1 cut(s)	980
BspTI	CTTAAG	1 cut(s)	149
BsrDI	GCAATG	4 cut(s)	90, 246, 595, 1395
BsrFI	RCCGGY	1 cut(s)	58
BsrI	ACTGG	9 cut(s)	564, 645, 766, 851, 937, 973, 991, 1237, 1482
BssAI	RCCGGY	1 cut(s)	58
BssECI	CCNNGG	4 cut(s)	117, 528, 648, 1246
BssHII	GCGCGC	1 cut(s)	154
BssMI	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
BssNAI	GTATAC	1 cut(s)	513
BssNI	GRCGYC	1 cut(s)	644
BssT1I	CCWWGG	3 cut(s)	117, 528, 648
Bst1107I	GTATAC	1 cut(s)	513
Bst2UI	CCWGG	3 cut(s)	135, 354, 696
Bst4CI	ACNGT	4 cut(s)	187, 1184, 1247, 1410
Bst6I	CTCTTC	3 cut(s)	72, 377, 1183
BstACI	GRCGYC	1 cut(s)	644
BstAFI	CTTAAG	1 cut(s)	149
BstAPI	GCANNNNNTGC	1 cut(s)	1028
BstBAI	YACGTR	1 cut(s)	1103
BstC8I	GCNNGC	3 cut(s)	156, 216, 493
BstDEI	CTNAG	5 cut(s)	171, 801, 807, 824, 1488
BstDSI	CCRYGG	2 cut(s)	648, 1246
BstF5I	GGATG	4 cut(s)	427, 895, 1024, 1119
BstFNI	CGCG	2 cut(s)	156, 608
BstH2I	RGCGCY	1 cut(s)	296
BstHHI	GCGC	4 cut(s)	156, 158, 295, 1314
BstKTI	GATC	9 cut(s)	433, 556, 621, 686, 701, 742, 817, 1015, 1220
BstMAI	GTCTC	2 cut(s)	72, 398
BstMBI	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
BstMCI	CGRYCG	1 cut(s)	686
BstMWI	GCNNNNNNNGC	2 cut(s)	151, 1028
BstNI	CCWGG	3 cut(s)	135, 354, 696
BstNSI	RCATGY	1 cut(s)	632
BstPAI	GACNNNNGTC	1 cut(s)	1248
BstSCI	CCNGG	3 cut(s)	133, 352, 694
BstSFI	CTRYAG	1 cut(s)	1032
BstUI	CGCG	2 cut(s)	156, 608
BstV1I	GCAGC	4 cut(s)	763, 766, 887, 1018
BstV2I	GAAGAC	2 cut(s)	119, 249
BstX2I	RGATCY	3 cut(s)	553, 698, 1217
BstXI	CCANNNNNNTGG	1 cut(s)	991
BstYI	RGATCY	3 cut(s)	553, 698, 1217
BstZ17I	GTATAC	1 cut(s)	513
BsuRI	GGCC	6 cut(s)	58, 89, 218, 357, 526, 1164
BtgI	CCRYGG	2 cut(s)	648, 1246
BtsCI	GGATG	4 cut(s)	427, 895, 1024, 1119
BtsIMutI	CAGTG	1 cut(s)	984
BtuMI	TCGCGA	1 cut(s)	608
BveI	ACCTGC	2 cut(s)	496, 968
Cac8I	GCNNGC	3 cut(s)	156, 216, 493
CfoI	GCGC	4 cut(s)	156, 158, 295, 1314
Cfr10I	RCCGGY	1 cut(s)	58
Cfr13I	GGNCC	4 cut(s)	179, 302, 525, 1250
CseI	GACGC	1 cut(s)	633
Csp6I	GTAC	4 cut(s)	8, 124, 638, 1073
CviAII	CATG	8 cut(s)	54, 445, 522, 629, 649, 788, 818, 1498
CviQI	GTAC	4 cut(s)	8, 124, 638, 1073
DdeI	CTNAG	5 cut(s)	171, 801, 807, 824, 1488
DpnI	GATC	9 cut(s)	432, 555, 620, 685, 700, 741, 816, 1014, 1219
DpnII	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
DraIII	CACNNNGTG	1 cut(s)	1106
DriI	GACNNNNNGTC	1 cut(s)	1276
EaeI	YGGCCR	5 cut(s)	56, 87, 216, 355, 1162
Eam1104I	CTCTTC	3 cut(s)	72, 377, 1183
Eam1105I	GACNNNNNGTC	1 cut(s)	1276
EarI	CTCTTC	3 cut(s)	72, 377, 1183
EciI	GGCGGA	1 cut(s)	1335
Eco130I	CCWWGG	3 cut(s)	117, 528, 648
Eco24I	GRGCYC	1 cut(s)	1139
Eco47I	GGWCC	3 cut(s)	179, 302, 1250
Eco72I	CACGTG	1 cut(s)	1103
Eco88I	CYCGRG	2 cut(s)	879, 1305
EcoRII	CCWGG	3 cut(s)	133, 352, 694
EcoT14I	CCWWGG	3 cut(s)	117, 528, 648
EcoT22I	ATGCAT	1 cut(s)	147
EcoT38I	GRGCYC	1 cut(s)	1139
ErhI	CCWWGG	3 cut(s)	117, 528, 648
FaeI	CATG	8 cut(s)	57, 448, 525, 632, 652, 791, 821, 1501
FaqI	GGGAC	1 cut(s)	1419
FatI	CATG	8 cut(s)	53, 444, 521, 628, 648, 787, 817, 1497
FauI	CCCGC	1 cut(s)	770
FbaI	TGATCA	2 cut(s)	739, 1012
FblI	GTMKAC	1 cut(s)	512
Fnu4HI	GCNGC	6 cut(s)	358, 496, 777, 780, 876, 1032
FokI	GGATG	4 cut(s)	434, 902, 1031, 1126
FriOI	GRGCYC	1 cut(s)	1139
Fsp4HI	GCNGC	6 cut(s)	358, 496, 777, 780, 876, 1032
FspBI	CTAG	2 cut(s)	374, 413
GlaI	GCGC	4 cut(s)	155, 157, 294, 1313
GluI	GCNGC	6 cut(s)	358, 496, 777, 780, 876, 1032
GsuI	CTGGAG	3 cut(s)	834, 954, 1167
HaeII	RGCGCY	1 cut(s)	296
HaeIII	GGCC	6 cut(s)	58, 89, 218, 357, 526, 1164
HapII	CCGG	2 cut(s)	59, 1365
HgaI	GACGC	1 cut(s)	633
HhaI	GCGC	4 cut(s)	156, 158, 295, 1314
Hin1I	GRCGYC	1 cut(s)	644
Hin1II	CATG	8 cut(s)	57, 448, 525, 632, 652, 791, 821, 1501
Hin6I	GCGC	4 cut(s)	154, 156, 293, 1312
HinP1I	GCGC	4 cut(s)	154, 156, 293, 1312
HincII	GTYRAC	1 cut(s)	565
HindII	GTYRAC	1 cut(s)	565
HinfI	GANTC	7 cut(s)	34, 226, 387, 826, 847, 1122, 1172
HpaII	CCGG	2 cut(s)	59, 1365
HphI	GGTGA	5 cut(s)	192, 589, 803, 1022, 1407
Hpy166II	GTNNAC	6 cut(s)	305, 513, 565, 580, 952, 1073
Hpy188I	TCNGA	4 cut(s)	174, 553, 804, 1127
Hpy8I	GTNNAC	6 cut(s)	305, 513, 565, 580, 952, 1073
HpyAV	CCTTC	7 cut(s)	472, 966, 1049, 1194, 1432, 1473, 1487
HpyCH4III	ACNGT	4 cut(s)	187, 1184, 1247, 1410
HpyCH4IV	ACGT	2 cut(s)	1102, 1129
HpyCH4V	TGCA	6 cut(s)	145, 548, 782, 875, 1022, 1034
HpyF10VI	GCNNNNNNNGC	2 cut(s)	151, 1028
HpyF3I	CTNAG	5 cut(s)	171, 801, 807, 824, 1488
HpySE526I	ACGT	2 cut(s)	1102, 1129
Hsp92I	GRCGYC	1 cut(s)	644
Hsp92II	CATG	8 cut(s)	57, 448, 525, 632, 652, 791, 821, 1501
HspAI	GCGC	4 cut(s)	154, 156, 293, 1312
Kpn2I	TCCGGA	1 cut(s)	1364
Ksp22I	TGATCA	2 cut(s)	739, 1012
Kzo9I	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
LguI	GCTCTTC	1 cut(s)	72
LmnI	GCTCC	9 cut(s)	211, 290, 329, 773, 1058, 1287, 1309, 1348, 1367
Lsp1109I	GCAGC	4 cut(s)	763, 766, 887, 1018
LweI	GCATC	3 cut(s)	154, 557, 1009
MaeI	CTAG	2 cut(s)	374, 413
MaeII	ACGT	2 cut(s)	1102, 1129
MaeIII	GTNAC	1 cut(s)	791
MalI	GATC	9 cut(s)	432, 555, 620, 685, 700, 741, 816, 1014, 1219
MboI	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
MboII	GAAGA	9 cut(s)	59, 124, 249, 394, 481, 553, 1200, 1212, 1493
MfeI	CAATTG	1 cut(s)	308
MflI	RGATCY	3 cut(s)	553, 698, 1217
MhlI	GDGCHC	1 cut(s)	1139
MlsI	TGGCCA	2 cut(s)	89, 218
MluCI	AATT	7 cut(s)	39, 308, 708, 783, 830, 916, 1083
MluNI	TGGCCA	2 cut(s)	89, 218
MlyI	GAGTC	3 cut(s)	28, 835, 841
MmeI	TCCRAC	3 cut(s)	206, 399, 1352
Mox20I	TGGCCA	2 cut(s)	89, 218
Mph1103I	ATGCAT	1 cut(s)	147
MroI	TCCGGA	1 cut(s)	1364
MroXI	GAANNNNTTC	3 cut(s)	256, 1121, 1171
MscI	TGGCCA	2 cut(s)	89, 218
MseI	TTAA	6 cut(s)	150, 506, 758, 894, 1044, 1335
MslI	CAYNNNNRTG	1 cut(s)	905
Msp20I	TGGCCA	2 cut(s)	89, 218
MspA1I	CMGCKG	1 cut(s)	1355
MspCI	CTTAAG	1 cut(s)	149
MspI	CCGG	2 cut(s)	59, 1365
MspR9I	CCNGG	3 cut(s)	135, 354, 696
MunI	CAATTG	1 cut(s)	308
MvaI	CCWGG	3 cut(s)	135, 354, 696
MvnI	CGCG	2 cut(s)	156, 608
MwoI	GCNNNNNNNGC	2 cut(s)	151, 1028
NcoI	CCATGG	1 cut(s)	648
NdeII	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
NlaIII	CATG	8 cut(s)	57, 448, 525, 632, 652, 791, 821, 1501
NlaIV	GGNNCC	5 cut(s)	180, 303, 700, 982, 1363
NmuCI	GTSAC	1 cut(s)	791
NruI	TCGCGA	1 cut(s)	608
NsiI	ATGCAT	1 cut(s)	147
NspI	RCATGY	1 cut(s)	632
PaeR7I	CTCGAG	1 cut(s)	879
PaqCI	CACCTGC	1 cut(s)	968
PauI	GCGCGC	1 cut(s)	154
PciSI	GCTCTTC	1 cut(s)	72
PdmI	GAANNNNTTC	3 cut(s)	256, 1121, 1171
PfeI	GAWTC	4 cut(s)	226, 387, 1122, 1172
PfoI	TCCNGGA	1 cut(s)	133
PkrI	GCNGC	6 cut(s)	359, 497, 778, 781, 877, 1033
Ple19I	CGATCG	1 cut(s)	686
PleI	GAGTC	3 cut(s)	28, 834, 841
PmaCI	CACGTG	1 cut(s)	1103
PmlI	CACGTG	1 cut(s)	1103
PpsI	GAGTC	3 cut(s)	28, 834, 841
Ppu21I	YACGTR	1 cut(s)	1103
PshAI	GACNNNNGTC	1 cut(s)	1248
PsiI	TTATAA	1 cut(s)	914
Psp6I	CCWGG	3 cut(s)	133, 352, 694
PspCI	CACGTG	1 cut(s)	1103
PspGI	CCWGG	3 cut(s)	133, 352, 694
PspN4I	GGNNCC	5 cut(s)	180, 303, 700, 982, 1363
PspPI	GGNCC	4 cut(s)	179, 302, 525, 1250
PspXI	VCTCGAGB	1 cut(s)	879
PstI	CTGCAG	1 cut(s)	1036
PsuI	RGATCY	3 cut(s)	553, 698, 1217
PteI	GCGCGC	1 cut(s)	154
PvuI	CGATCG	1 cut(s)	686
PvuII	CAGCTG	1 cut(s)	1355
RgaI	GCGATCGC	1 cut(s)	686
RruI	TCGCGA	1 cut(s)	608
RsaI	GTAC	4 cut(s)	9, 125, 639, 1074
RsaNI	GTAC	4 cut(s)	8, 124, 638, 1073
RseI	CAYNNNNRTG	1 cut(s)	905
SapI	GCTCTTC	1 cut(s)	72
SaqAI	TTAA	6 cut(s)	150, 506, 758, 894, 1044, 1335
SatI	GCNGC	6 cut(s)	358, 496, 777, 780, 876, 1032
Sau3AI	GATC	9 cut(s)	430, 553, 618, 683, 698, 739, 814, 1012, 1217
Sau96I	GGNCC	4 cut(s)	179, 302, 525, 1250
ScaI	AGTACT	1 cut(s)	639
SchI	GAGTC	3 cut(s)	28, 835, 841
ScrFI	CCNGG	3 cut(s)	135, 354, 696
SduI	GDGCHC	1 cut(s)	1139
SfaAI	GCGATCGC	1 cut(s)	686
SfaNI	GCATC	3 cut(s)	154, 557, 1009
SfcI	CTRYAG	1 cut(s)	1032
Sfr274I	CTCGAG	1 cut(s)	879
SgfI	GCGATCGC	1 cut(s)	686
SinI	GGWCC	3 cut(s)	179, 302, 1250
SlaI	CTCGAG	1 cut(s)	879
SmiMI	CAYNNNNRTG	1 cut(s)	905
SmlI	CTYRAG	2 cut(s)	149, 879
SmoI	CTYRAG	2 cut(s)	149, 879
Sse9I	AATT	7 cut(s)	39, 308, 708, 783, 830, 916, 1083
SsiI	CCGC	6 cut(s)	358, 496, 763, 1206, 1346, 1418
SspMI	CTAG	2 cut(s)	374, 413
StyD4I	CCNGG	3 cut(s)	133, 352, 694
StyI	CCWWGG	3 cut(s)	117, 528, 648
TaaI	ACNGT	4 cut(s)	187, 1184, 1247, 1410
TaiI	ACGT	2 cut(s)	1105, 1132
TaqI	TCGA	2 cut(s)	617, 880
TasI	AATT	7 cut(s)	39, 308, 708, 783, 830, 916, 1083
TatI	WGTACW	1 cut(s)	637
TauI	GCSGC	2 cut(s)	360, 498
TfiI	GAWTC	4 cut(s)	226, 387, 1122, 1172
Tru1I	TTAA	6 cut(s)	150, 506, 758, 894, 1044, 1335
Tru9I	TTAA	6 cut(s)	150, 506, 758, 894, 1044, 1335
TscAI	CASTG	1 cut(s)	991
TseFI	GTSAC	1 cut(s)	791
TseI	GCWGC	4 cut(s)	776, 779, 875, 1031
Tsp45I	GTSAC	1 cut(s)	791
TspDTI	ATGAA	6 cut(s)	249, 257, 395, 612, 776, 1130
TspGWI	ACGGA	1 cut(s)	1448
TspRI	CASTG	1 cut(s)	991
Vha464I	CTTAAG	1 cut(s)	149
VpaK11BI	GGWCC	3 cut(s)	179, 302, 1250
XapI	RAATTY	1 cut(s)	1083
XbaI	TCTAGA	1 cut(s)	373
XceI	RCATGY	1 cut(s)	632
XhoI	CTCGAG	1 cut(s)	879
XmiI	GTMKAC	1 cut(s)	512
XmnI	GAANNNNTTC	3 cut(s)	256, 1121, 1171
XspI	CTAG	2 cut(s)	374, 413
ZrmI	AGTACT	1 cut(s)	639
Zsp2I	ATGCAT	1 cut(s)	147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.