Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002971

Physical Location & Seq

Genomic Coordinates Reverse (-)

2295190 .. 2300919

Gene Structure 5730 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1031800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGCATTGCTCTCTTCCCATGGCTGCCACTGCAACTGCTGCTGGTCCTCGATATGCTCCGGAGGACCCAACTCTCCCCAAACCATGGAGAGGTCTGGTTGATGGTAAAACTGGGTATCTTTACTTCTGGAACCCGGAGACCAACGTTACCCAATATGATAGGCCAACGAGCTCAGCTCCTCCATCAAAGCCCTCTTCTGCACAGTCTATAAGCTCCTCGGTTCATGTTCAACAATCTTCACAAGGGCAACGTCGTCCTGATGAGGGAGATGATAGGTATAGCAGAGGCAGCAATGGTGGCTCGAAATTTGACACAGGATCAAGAACTCAGCAGACTGCAAGAAGTGCAACAGTTTCTCATAATACCCCAAGTGGGACACTAAGTTCACATGGAGGATCTTCTGTGAAAGGAAATGGATCAGACACTGGAAGTGGTTTGTCTGCTGAGGCTTATAGTCTCCGCCATGAAATATCAGTTGTTGGTGATAATGTGCCTCCACCATTCACTTCATTTGAATCTACTGGCTTCCCATCTGAGATTCTTAGAGAGGTACAAAGTGCAGGTTTCTCTGCCCCAACTCCAATTCAAGCACAATCTTGGCCGGTTGCTCTTCAAAGTAGAGATATAGTAGCCATTGCTAAAACTGGTTCGGGGAAGACCTTGGGGTATTTGCTTCCAGGATTCATCCATCTCAAGCGCACCCGTAATAACCCTCAGATGGGTCCAACTGTATTGGTTTTATCACCAACTAGGGAGCTGGCAACACAGATTCAAGATGAGGCAGTGAAGTTTGGGAAGTCATCAAGGATATCTTGTGTGTGTTTGTATGGAGGAGCTCCAAAGGGACCACAGTTGAGAGATATTGACCGAGGAGCAGATGTTGTGGTTGCCACTCCTGGCCGCTTGAATGATATTCTTGAAATGAAGAGAATCAGTCTCCATCAAGTTTCTTATTTAGTGCTGGATGAGGCTGACCGGATGCTGGACATGGGGTTTGAACCACAAATAAGGAAGATTGTGAAGGAGATACCTGCTCGTCGCCAGACCCTAATGTATACCGCAACTTGGCCAAAGGAGGTTCGGAAGATTGCAGCGGATCTACTGGTCAAACCTGTTCAGGTTAACATTGGCAATGTTGATGAACTGGTTGCGAATAAGTCTATCACACAGTACGTTGAAGTACTGACCTCAATGGAGAAGCACAGACGACTGGAGCAGATATTGCGATCCCAAGAGCCAGGGTCTAAGATAATTATCTTTTGTTCAACTAAGAAGATGTGCGATCAACTTTCTCGCAATCTAACCCGCCAGTTTGGAGCTGCTGCAATTCATGGCGACAAATCTCAGAGTGAGAGGGATTATGTGTTGAATCAATTTCGCTCTGGGAGGACTCCAATTCTTGTAGCAACTGACGTTGCAGCTCGAGGATTGGACATAAAGGACATAAGGGTGGTTATCAACTACGACTTCCCTACTGGAGTAGAGGATTATGTTCACAGGATTGGGAGAACTGGAAGAGCGGGCGCCACTGGCCTGGCTTATACATTTTTTGGTGATCAGGATGCCAAGTATGCATCAGATCTCATCAAAGTTTTGGAAGGTGCAAATCAGTGTGTCCCCCCAGAAGTTCGTGAGCTGGCTTCCCGTGGTGGTGGGATGAACAGGTTCAGACGTTGGGGTTCTGGGCCCAGTGGTGGTCGTGATGGGGGTCGTGGGCAAAATGATTCAAGTTATGGTGGAAGGGATGGTGGAAGGGGAGGAGGATATGGATTTACTCCCTCCTCTGGCAGGTATGGTCAATCCAATGGGTATGACAGAGGCCGCAGTCCCAGCCCTGTCAAGGCGTCAGGATATAGAGCCCGTAGCCGTAGTCCTGAGAGGTTTGACAAAGCTCTGCCAACAGGTGGTGGCTCTGCAGGTGGTTGGAGTTTCCACAAAGCAATGATGGAAAGGGAGCGGTCATCGCCTCCACGTAGACGCAGTGCTGAAGAAGCTGAGGAGGGAATGATACATCCAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009628 GO:0009651 GO:0009987 GO:0010501 GO:0016070 GO:0016301 GO:0016310 GO:0016462 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031974 GO:0031981 GO:0034641 GO:0036211 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046777 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140096 GO:0140098 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

674

Amino Acids

73.02

Weight (kDa)

9.55

Isoelectric Point (pI)

53.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	25 - 55	1.1e-06	WW domain
DEAD	PF00270	193 - 362	1.2e-48	DEAD/DEAH box helicase
ResIII	PF04851	205 - 356	2.1e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	400 - 508	2.3e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01540 AT3G01540 AT3G01540 AT3G01540 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610
fragaria_vesca	FvH4_3g33550 FvH4_6g50990
malus_domestica	MD09G1029800.v1.1 MD17G1031800.v1.1
prunus_persica	Prupe.3G287500_v2.0.a1 Prupe.3G287500_v2.0.a1
pyrus_communis	pycom17g02940
rosa_chinensis	RchiOBHm_Chr2g0172491 RchiOBHm_Chr3g0496011
rosa_laevigata	RLG00000022110 RLG00000026510
rosa_multiflora	Rmu_co8447803.1_g000001 Rmu_sc0008577.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079810 Rroxscaffold_6G00401610
rosa_rugosa	Rorug02G0559500
rosa_samantha	Rh1CG060200 Rh2AG635200 Rh2BG648700 Rh2CG615300 Rh2DG663200 Rh3AG325700 Rh5DG348900 Rh5DG552200 Rh7AG279200 Rh7CG298300
rosa_wichuraiana	Rw2G052550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1907
Acc36I	ACCTGC	4 cut(s)	551, 1039, 1779, 1907
AccB1I	GGYRCC	1 cut(s)	1523
AccB7I	CCANNNNNTGG	2 cut(s)	84, 1904
AccBSI	CCGCTC	2 cut(s)	1520, 1957
AccI	GTMKAC	2 cut(s)	1055, 1975
AccIII	TCCGGA	1 cut(s)	58
AciI	CCGC	8 cut(s)	460, 901, 1059, 1094, 1306, 1520, 1822, 1957
AclI	AACGTT	1 cut(s)	144
AclWI	GGATC	5 cut(s)	325, 403, 424, 1104, 1221
AcoI	YGGCCR	3 cut(s)	599, 898, 1067
AcsI	RAATTY	1 cut(s)	305
AcuI	CTGAAG	1 cut(s)	2007
AcyI	GRCGYC	2 cut(s)	1524, 1844
AfaI	GTAC	3 cut(s)	552, 1172, 1182
AjnI	CCWGG	4 cut(s)	676, 895, 1237, 1533
AloI	GAACNNNNNNTCC	2 cut(s)	367, 399
Alw21I	GWGCWC	2 cut(s)	173, 838
Alw26I	GTCTC	3 cut(s)	131, 461, 941
AlwI	GGATC	5 cut(s)	325, 403, 424, 1104, 1221
AlwNI	CAGNNNCTG	1 cut(s)	425
Ama87I	CYCGRG	1 cut(s)	1422
Aor13HI	TCCGGA	1 cut(s)	58
AoxI	GGCC	7 cut(s)	161, 599, 898, 1067, 1531, 1685, 1819
ApaI	GGGCCC	1 cut(s)	1689
ApeKI	GCWGC	7 cut(s)	23, 38, 288, 1091, 1319, 1322, 1418
ApoI	RAATTY	1 cut(s)	305
ArsI	GACNNNNNNTTYG	2 cut(s)	977, 1009
Asp700I	GAANNNNTTC	1 cut(s)	1664
AspLEI	GCGC	2 cut(s)	699, 1526
AspS9I	GGNCC	6 cut(s)	44, 64, 722, 845, 1685, 1686
AsuC2I	CCSGG	1 cut(s)	134
AsuHPI	GGTGA	3 cut(s)	494, 735, 1565
AvaI	CYCGRG	1 cut(s)	1422
AvaII	GGWCC	4 cut(s)	44, 64, 722, 845
BaeGI	GKGCMC	1 cut(s)	1689
BalI	TGGCCA	1 cut(s)	1069
BanI	GGYRCC	1 cut(s)	1523
BanII	GRGCYC	4 cut(s)	173, 838, 1689, 1861
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
BbsI	GAAGAC	1 cut(s)	662
Bbv12I	GWGCWC	2 cut(s)	173, 838
BbvCI	CCTCAGC	2 cut(s)	444, 1995
BbvI	GCAGC	7 cut(s)	10, 25, 300, 1103, 1306, 1309, 1430
BccI	CCATC	9 cut(s)	95, 190, 538, 696, 712, 948, 1697, 1739, 1939
BceAI	ACGGC	1 cut(s)	1851
BciT130I	CCWGG	4 cut(s)	678, 897, 1239, 1535
BclI	TGATCA	1 cut(s)	1555
BcnI	CCSGG	1 cut(s)	134
BcoDI	GTCTC	3 cut(s)	131, 461, 941
BfaI	CTAG	1 cut(s)	750
BfmI	CTRYAG	1 cut(s)	1914
BfoI	RGCGCY	1 cut(s)	1527
BfuAI	ACCTGC	4 cut(s)	551, 1039, 1779, 1907
BglII	AGATCT	1 cut(s)	1579
BisI	GCNGC	9 cut(s)	24, 39, 289, 901, 1092, 1320, 1323, 1419, 1822
BlpI	GCTNAGC	1 cut(s)	172
BlsI	GCNGC	9 cut(s)	25, 40, 290, 902, 1093, 1321, 1324, 1420, 1823
BmcAI	AGTACT	1 cut(s)	1182
Bme1390I	CCNGG	5 cut(s)	134, 678, 897, 1239, 1535
Bme18I	GGWCC	4 cut(s)	44, 64, 722, 845
BmeT110I	CYCGRG	1 cut(s)	1422
BmgT120I	GGNCC	6 cut(s)	44, 64, 722, 845, 1685, 1686
BmiI	GGNNCC	6 cut(s)	66, 131, 723, 846, 1525, 1687
BmrFI	CCNGG	5 cut(s)	134, 678, 897, 1239, 1535
BmrI	ACTGGG	2 cut(s)	120, 1683
BmsI	GCATC	3 cut(s)	969, 1552, 1583
BmuI	ACTGGG	2 cut(s)	120, 1683
BpiI	GAAGAC	1 cut(s)	662
BplI	GAGNNNNNCTC	2 cut(s)	160, 192
BpmI	CTGGAG	2 cut(s)	1232, 1497
Bpu10I	CCTNAGC	2 cut(s)	444, 1995
Bpu1102I	GCTNAGC	1 cut(s)	172
BpuEI	CTTGAG	1 cut(s)	677
BpuMI	CCSGG	1 cut(s)	134
BsaAI	YACGTR	1 cut(s)	1973
BsaBI	GATNNNNATC	1 cut(s)	1253
BsaHI	GRCGYC	2 cut(s)	1524, 1844
BsaI	GGTCTC	1 cut(s)	131
BsaJI	CCNNGG	7 cut(s)	18, 83, 216, 660, 868, 1238, 1645
BsaWI	WCCGGW	2 cut(s)	58, 975
BsaXI	ACNNNNNCTCC	2 cut(s)	864, 894
Bse118I	RCCGGY	1 cut(s)	601
Bse3DI	GCAATG	5 cut(s)	4, 298, 633, 1138, 1947
Bse8I	GATNNNNATC	1 cut(s)	1253
BseAI	TCCGGA	1 cut(s)	58
BseBI	CCWGG	4 cut(s)	678, 897, 1239, 1535
BseDI	CCNNGG	7 cut(s)	18, 83, 216, 660, 868, 1238, 1645
BseGI	GGATG	7 cut(s)	684, 970, 984, 1567, 1662, 1750, 2011
BseJI	GATNNNNATC	1 cut(s)	1253
BseMI	GCAATG	5 cut(s)	4, 298, 633, 1138, 1947
BseMII	CTCAG	8 cut(s)	186, 341, 435, 525, 728, 1358, 1866, 1986
BseRI	GAGGAG	7 cut(s)	168, 205, 846, 885, 1771, 1773, 2012
BseSI	GKGCMC	1 cut(s)	1689
BseXI	GCAGC	7 cut(s)	10, 25, 300, 1103, 1306, 1309, 1430
BseYI	CCCAGC	1 cut(s)	1829
BsgI	GTGCAG	2 cut(s)	183, 579
BshFI	GGCC	7 cut(s)	163, 601, 900, 1069, 1533, 1687, 1821
BshNI	GGYRCC	1 cut(s)	1523
BsiHKAI	GWGCWC	2 cut(s)	173, 838
BsiHKCI	CYCGRG	1 cut(s)	1422
BsiSI	CCGG	4 cut(s)	59, 134, 602, 976
BslFI	GGGAC	4 cut(s)	388, 858, 1601, 1812
BsmAI	GTCTC	3 cut(s)	131, 461, 941
BsmFI	GGGAC	4 cut(s)	388, 858, 1601, 1812
BsnI	GGCC	7 cut(s)	163, 601, 900, 1069, 1533, 1687, 1821
Bso31I	GGTCTC	1 cut(s)	131
BsoBI	CYCGRG	1 cut(s)	1422
Bsp120I	GGGCCC	1 cut(s)	1685
Bsp1286I	GDGCHC	4 cut(s)	173, 838, 1689, 1861
Bsp13I	TCCGGA	1 cut(s)	58
Bsp143I	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
Bsp1720I	GCTNAGC	1 cut(s)	172
Bsp19I	CCATGG	2 cut(s)	18, 83
BspACI	CCGC	8 cut(s)	460, 901, 1059, 1094, 1306, 1520, 1822, 1957
BspANI	GGCC	7 cut(s)	163, 601, 900, 1069, 1533, 1687, 1821
BspCNI	CTCAG	8 cut(s)	185, 340, 436, 526, 727, 1357, 1867, 1987
BspEI	TCCGGA	1 cut(s)	58
BspLI	GGNNCC	6 cut(s)	66, 131, 723, 846, 1525, 1687
BspMAI	CTGCAG	1 cut(s)	1918
BspMI	ACCTGC	4 cut(s)	551, 1039, 1779, 1907
BspPI	GGATC	5 cut(s)	325, 403, 424, 1104, 1221
BspQI	GCTCTTC	2 cut(s)	615, 1510
BspT107I	GGYRCC	1 cut(s)	1523
BspTNI	GGTCTC	1 cut(s)	131
BsrBI	CCGCTC	2 cut(s)	1520, 1957
BsrDI	GCAATG	5 cut(s)	4, 298, 633, 1138, 1947
BsrFI	RCCGGY	1 cut(s)	601
BssAI	RCCGGY	1 cut(s)	601
BssECI	CCNNGG	7 cut(s)	18, 83, 216, 660, 868, 1238, 1645
BssMI	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
BssNAI	GTATAC	1 cut(s)	1056
BssNI	GRCGYC	2 cut(s)	1524, 1844
BssT1I	CCWWGG	3 cut(s)	18, 83, 660
Bst1107I	GTATAC	1 cut(s)	1056
Bst2UI	CCWGG	4 cut(s)	678, 897, 1239, 1535
Bst4CI	ACNGT	5 cut(s)	204, 352, 730, 852, 1170
Bst6I	CTCTTC	5 cut(s)	18, 199, 615, 920, 1510
BstACI	GRCGYC	2 cut(s)	1524, 1844
BstAPI	GCANNNNNTGC	3 cut(s)	38, 344, 1222
BstBAI	YACGTR	1 cut(s)	1973
BstC8I	GCNNGC	3 cut(s)	759, 1522, 1638
BstDSI	CCRYGG	3 cut(s)	18, 83, 1645
BstENI	CCTNNNNNAGG	1 cut(s)	1786
BstF5I	GGATG	7 cut(s)	684, 970, 984, 1567, 1662, 1750, 2011
BstH2I	RGCGCY	1 cut(s)	1527
BstHHI	GCGC	2 cut(s)	699, 1526
BstKTI	GATC	8 cut(s)	320, 398, 419, 1099, 1229, 1285, 1558, 1582
BstMAI	GTCTC	3 cut(s)	131, 461, 941
BstMBI	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
BstNI	CCWGG	4 cut(s)	678, 897, 1239, 1535
BstSCI	CCNGG	5 cut(s)	132, 676, 895, 1237, 1533
BstSFI	CTRYAG	1 cut(s)	1914
BstSLI	GKGCMC	1 cut(s)	1689
BstV1I	GCAGC	7 cut(s)	10, 25, 300, 1103, 1306, 1309, 1430
BstV2I	GAAGAC	1 cut(s)	662
BstX2I	RGATCY	3 cut(s)	395, 1096, 1579
BstXI	CCANNNNNNTGG	1 cut(s)	1534
BstYI	RGATCY	3 cut(s)	395, 1096, 1579
BstZ17I	GTATAC	1 cut(s)	1056
BsuRI	GGCC	7 cut(s)	163, 601, 900, 1069, 1533, 1687, 1821
BtgI	CCRYGG	3 cut(s)	18, 83, 1645
BtgZI	GCGATG	1 cut(s)	1947
BtsCI	GGATG	7 cut(s)	684, 970, 984, 1567, 1662, 1750, 2011
BtsI	GCAGTG	3 cut(s)	27, 789, 1987
BtsIMutI	CAGTG	7 cut(s)	27, 423, 789, 1527, 1616, 1696, 1987
BveI	ACCTGC	4 cut(s)	551, 1039, 1779, 1907
Cac8I	GCNNGC	3 cut(s)	759, 1522, 1638
CaiI	CAGNNNCTG	1 cut(s)	425
CfoI	GCGC	2 cut(s)	699, 1526
Cfr10I	RCCGGY	1 cut(s)	601
Cfr13I	GGNCC	6 cut(s)	44, 64, 722, 845, 1685, 1686
CseI	GACGC	2 cut(s)	1833, 1986
Csp6I	GTAC	3 cut(s)	551, 1171, 1181
CviAII	CATG	7 cut(s)	19, 84, 224, 389, 464, 988, 1331
CviQI	GTAC	3 cut(s)	551, 1171, 1181
DinI	GGCGCC	1 cut(s)	1525
DpnI	GATC	8 cut(s)	319, 397, 418, 1098, 1228, 1284, 1557, 1581
DpnII	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
EaeI	YGGCCR	3 cut(s)	599, 898, 1067
Eam1104I	CTCTTC	5 cut(s)	18, 199, 615, 920, 1510
EarI	CTCTTC	5 cut(s)	18, 199, 615, 920, 1510
EciI	GGCGGA	1 cut(s)	449
Ecl136II	GAGCTC	2 cut(s)	171, 836
Eco130I	CCWWGG	3 cut(s)	18, 83, 660
Eco24I	GRGCYC	4 cut(s)	173, 838, 1689, 1861
Eco31I	GGTCTC	1 cut(s)	131
Eco32I	GATATC	1 cut(s)	810
Eco47I	GGWCC	4 cut(s)	44, 64, 722, 845
Eco53kI	GAGCTC	2 cut(s)	171, 836
Eco57I	CTGAAG	1 cut(s)	2007
Eco88I	CYCGRG	1 cut(s)	1422
EcoICRI	GAGCTC	2 cut(s)	171, 836
EcoNI	CCTNNNNNAGG	1 cut(s)	1786
EcoO109I	RGGNCCY	1 cut(s)	64
EcoRII	CCWGG	4 cut(s)	676, 895, 1237, 1533
EcoRV	GATATC	1 cut(s)	810
EcoT14I	CCWWGG	3 cut(s)	18, 83, 660
EcoT22I	ATGCAT	2 cut(s)	6, 1576
EcoT38I	GRGCYC	4 cut(s)	173, 838, 1689, 1861
EgeI	GGCGCC	1 cut(s)	1525
EheI	GGCGCC	1 cut(s)	1525
ErhI	CCWWGG	3 cut(s)	18, 83, 660
FaeI	CATG	7 cut(s)	22, 87, 227, 392, 467, 991, 1334
FalI	AAGNNNNNCTT	2 cut(s)	796, 828
FaqI	GGGAC	4 cut(s)	388, 858, 1601, 1812
FatI	CATG	7 cut(s)	18, 83, 223, 388, 463, 987, 1330
FauI	CCCGC	2 cut(s)	1313, 1513
FbaI	TGATCA	1 cut(s)	1555
FblI	GTMKAC	2 cut(s)	1055, 1975
Fnu4HI	GCNGC	9 cut(s)	24, 39, 289, 901, 1092, 1320, 1323, 1419, 1822
FokI	GGATG	7 cut(s)	671, 977, 991, 1574, 1669, 1757, 1998
FriOI	GRGCYC	4 cut(s)	173, 838, 1689, 1861
Fsp4HI	GCNGC	9 cut(s)	24, 39, 289, 901, 1092, 1320, 1323, 1419, 1822
FspBI	CTAG	1 cut(s)	750
GlaI	GCGC	2 cut(s)	698, 1525
GluI	GCNGC	9 cut(s)	24, 39, 289, 901, 1092, 1320, 1323, 1419, 1822
GsaI	CCCAGC	1 cut(s)	1833
GsuI	CTGGAG	2 cut(s)	1232, 1497
HaeII	RGCGCY	1 cut(s)	1527
HaeIII	GGCC	7 cut(s)	163, 601, 900, 1069, 1533, 1687, 1821
HapII	CCGG	4 cut(s)	59, 134, 602, 976
HgaI	GACGC	2 cut(s)	1833, 1986
HhaI	GCGC	2 cut(s)	699, 1526
Hin1I	GRCGYC	2 cut(s)	1524, 1844
Hin1II	CATG	7 cut(s)	22, 87, 227, 392, 467, 991, 1334
Hin6I	GCGC	2 cut(s)	697, 1524
HinP1I	GCGC	2 cut(s)	697, 1524
HincII	GTYRAC	1 cut(s)	1123
HindII	GTYRAC	1 cut(s)	1123
HinfI	GANTC	8 cut(s)	515, 538, 681, 769, 930, 1369, 1390, 1724
HpaI	GTTAAC	1 cut(s)	1123
HpaII	CCGG	4 cut(s)	59, 134, 602, 976
HphI	GGTGA	3 cut(s)	494, 735, 1565
Hpy166II	GTNNAC	5 cut(s)	386, 1056, 1123, 1495, 1976
Hpy188I	TCNGA	7 cut(s)	421, 535, 717, 1083, 1347, 1579, 1670
Hpy8I	GTNNAC	5 cut(s)	386, 1056, 1123, 1495, 1976
Hpy99I	CGWCG	2 cut(s)	255, 1041
HpyAV	CCTTC	4 cut(s)	1015, 1592, 1734, 1746
HpyCH4III	ACNGT	5 cut(s)	204, 352, 730, 852, 1170
HpyCH4IV	ACGT	6 cut(s)	144, 250, 1173, 1413, 1672, 1972
HpySE526I	ACGT	6 cut(s)	144, 250, 1173, 1413, 1672, 1972
Hsp92I	GRCGYC	2 cut(s)	1524, 1844
Hsp92II	CATG	7 cut(s)	22, 87, 227, 392, 467, 991, 1334
HspAI	GCGC	2 cut(s)	697, 1524
KasI	GGCGCC	1 cut(s)	1523
Kpn2I	TCCGGA	1 cut(s)	58
Ksp22I	TGATCA	1 cut(s)	1555
KspAI	GTTAAC	1 cut(s)	1123
Kzo9I	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
LguI	GCTCTTC	2 cut(s)	615, 1510
Lsp1109I	GCAGC	7 cut(s)	10, 25, 300, 1103, 1306, 1309, 1430
LweI	GCATC	3 cut(s)	969, 1552, 1583
MaeI	CTAG	1 cut(s)	750
MaeII	ACGT	6 cut(s)	144, 250, 1173, 1413, 1672, 1972
MaeIII	GTNAC	1 cut(s)	145
MalI	GATC	8 cut(s)	319, 397, 418, 1098, 1228, 1284, 1557, 1581
MbiI	CCGCTC	2 cut(s)	1520, 1957
MboI	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
MflI	RGATCY	3 cut(s)	395, 1096, 1579
MhlI	GDGCHC	4 cut(s)	173, 838, 1689, 1861
MlsI	TGGCCA	1 cut(s)	1069
MluCI	AATT	6 cut(s)	305, 582, 1251, 1326, 1373, 1395
MluNI	TGGCCA	1 cut(s)	1069
Mly113I	GGCGCC	1 cut(s)	1524
MlyI	GAGTC	1 cut(s)	1384
MmeI	TCCRAC	2 cut(s)	749, 1904
Mox20I	TGGCCA	1 cut(s)	1069
Mph1103I	ATGCAT	2 cut(s)	6, 1576
MroI	TCCGGA	1 cut(s)	58
MroXI	GAANNNNTTC	1 cut(s)	1664
MscI	TGGCCA	1 cut(s)	1069
MseI	TTAA	1 cut(s)	1122
MslI	CAYNNNNRTG	1 cut(s)	1448
Msp20I	TGGCCA	1 cut(s)	1069
MspA1I	CMGCKG	1 cut(s)	1094
MspI	CCGG	4 cut(s)	59, 134, 602, 976
MspR9I	CCNGG	5 cut(s)	134, 678, 897, 1239, 1535
MvaI	CCWGG	4 cut(s)	678, 897, 1239, 1535
NarI	GGCGCC	1 cut(s)	1524
NciI	CCSGG	1 cut(s)	134
NcoI	CCATGG	2 cut(s)	18, 83
NdeII	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
NlaIII	CATG	7 cut(s)	22, 87, 227, 392, 467, 991, 1334
NlaIV	GGNNCC	6 cut(s)	66, 131, 723, 846, 1525, 1687
NsiI	ATGCAT	2 cut(s)	6, 1576
PaeR7I	CTCGAG	1 cut(s)	1422
PaqCI	CACCTGC	1 cut(s)	1907
PciSI	GCTCTTC	2 cut(s)	615, 1510
PdmI	GAANNNNTTC	1 cut(s)	1664
PfeI	GAWTC	7 cut(s)	515, 538, 681, 769, 930, 1369, 1724
PflMI	CCANNNNNTGG	2 cut(s)	84, 1904
PfoI	TCCNGGA	1 cut(s)	676
PkrI	GCNGC	9 cut(s)	25, 40, 290, 902, 1093, 1321, 1324, 1420, 1823
PleI	GAGTC	1 cut(s)	1384
PluTI	GGCGCC	1 cut(s)	1527
PpsI	GAGTC	1 cut(s)	1384
Ppu21I	YACGTR	1 cut(s)	1973
PpuMI	RGGWCCY	1 cut(s)	64
Psp124BI	GAGCTC	2 cut(s)	173, 838
Psp1406I	AACGTT	1 cut(s)	144
Psp5II	RGGWCCY	1 cut(s)	64
Psp6I	CCWGG	4 cut(s)	676, 895, 1237, 1533
PspFI	CCCAGC	1 cut(s)	1829
PspGI	CCWGG	4 cut(s)	676, 895, 1237, 1533
PspN4I	GGNNCC	6 cut(s)	66, 131, 723, 846, 1525, 1687
PspOMI	GGGCCC	1 cut(s)	1685
PspPI	GGNCC	6 cut(s)	44, 64, 722, 845, 1685, 1686
PspPPI	RGGWCCY	1 cut(s)	64
PspXI	VCTCGAGB	1 cut(s)	1422
PstI	CTGCAG	1 cut(s)	1918
PstNI	CAGNNNCTG	1 cut(s)	425
PsuI	RGATCY	3 cut(s)	395, 1096, 1579
RsaI	GTAC	3 cut(s)	552, 1172, 1182
RsaNI	GTAC	3 cut(s)	551, 1171, 1181
RseI	CAYNNNNRTG	1 cut(s)	1448
SacI	GAGCTC	2 cut(s)	173, 838
SapI	GCTCTTC	2 cut(s)	615, 1510
SaqAI	TTAA	1 cut(s)	1122
SatI	GCNGC	9 cut(s)	24, 39, 289, 901, 1092, 1320, 1323, 1419, 1822
Sau3AI	GATC	8 cut(s)	317, 395, 416, 1096, 1226, 1282, 1555, 1579
Sau96I	GGNCC	6 cut(s)	44, 64, 722, 845, 1685, 1686
ScaI	AGTACT	1 cut(s)	1182
SchI	GAGTC	1 cut(s)	1384
ScrFI	CCNGG	5 cut(s)	134, 678, 897, 1239, 1535
SduI	GDGCHC	4 cut(s)	173, 838, 1689, 1861
SfaNI	GCATC	3 cut(s)	969, 1552, 1583
SfcI	CTRYAG	1 cut(s)	1914
SfoI	GGCGCC	1 cut(s)	1525
Sfr274I	CTCGAG	1 cut(s)	1422
SinI	GGWCC	4 cut(s)	44, 64, 722, 845
SlaI	CTCGAG	1 cut(s)	1422
SmiMI	CAYNNNNRTG	1 cut(s)	1448
SmlI	CTYRAG	2 cut(s)	692, 1422
SmoI	CTYRAG	2 cut(s)	692, 1422
Sse9I	AATT	6 cut(s)	305, 582, 1251, 1326, 1373, 1395
SsiI	CCGC	8 cut(s)	460, 901, 1059, 1094, 1306, 1520, 1822, 1957
SspDI	GGCGCC	1 cut(s)	1523
SspMI	CTAG	1 cut(s)	750
SstI	GAGCTC	2 cut(s)	173, 838
StyD4I	CCNGG	5 cut(s)	132, 676, 895, 1237, 1533
StyI	CCWWGG	3 cut(s)	18, 83, 660
TaaI	ACNGT	5 cut(s)	204, 352, 730, 852, 1170
TaiI	ACGT	6 cut(s)	147, 253, 1176, 1416, 1675, 1975
TaqI	TCGA	3 cut(s)	49, 302, 1423
TaqII	GACCGA	1 cut(s)	882
TasI	AATT	6 cut(s)	305, 582, 1251, 1326, 1373, 1395
TatI	WGTACW	1 cut(s)	1180
TauI	GCSGC	2 cut(s)	903, 1824
TfiI	GAWTC	7 cut(s)	515, 538, 681, 769, 930, 1369, 1724
Tru1I	TTAA	1 cut(s)	1122
Tru9I	TTAA	1 cut(s)	1122
TscAI	CASTG	7 cut(s)	34, 430, 789, 1534, 1616, 1696, 1987
TseI	GCWGC	7 cut(s)	23, 38, 288, 1091, 1319, 1322, 1418
TspDTI	ATGAA	8 cut(s)	212, 480, 498, 673, 938, 1155, 1319, 1673
TspRI	CASTG	7 cut(s)	34, 430, 789, 1534, 1616, 1696, 1987
Van91I	CCANNNNNTGG	2 cut(s)	84, 1904
VpaK11BI	GGWCC	4 cut(s)	44, 64, 722, 845
XagI	CCTNNNNNAGG	1 cut(s)	1786
XapI	RAATTY	1 cut(s)	305
XhoI	CTCGAG	1 cut(s)	1422
XmiI	GTMKAC	2 cut(s)	1055, 1975
XmnI	GAANNNNTTC	1 cut(s)	1664
XspI	CTAG	1 cut(s)	750
ZrmI	AGTACT	1 cut(s)	1182
Zsp2I	ATGCAT	2 cut(s)	6, 1576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.