Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002971

Physical Location & Seq

Genomic Coordinates Forward (+)

81008015 .. 81014227

Gene Structure 6213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022110

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGACAGACCCACCCAAGGGCGAGAGAGACACACCACATCACACCCCACCCCACCCGCACCAATCAGATCTATCACCGGTGTTTCACCTTCCGTTCACTCTCCAAAACGGCGCCGTCAGGATAGCGTTTAATTGCCATGGCTGCCACTGCAACTGCCTCTGCTGGCCTACAGGCACAGCTCCTCCATCAAAGGCTTCTTCTGCACAGTATATAAGTTCTTCTGTTCAAGTTCAACAATCTTCACAAGGACAACGTCGTGATGAGGGAGATGAAAGGTATGGCAGGGGCAGCAATGGTGGATCAAAATTTGAGGCAGGACCGAGGAATGTACAGAGTGCAAGAAGTGCACCACTTTCTTCTAACAATGGTCCAAGTGGGTCAGTTAGTGCACATGGAGGATCTGTTATTAGAGGAAATGGACCATCAGACTCTGGAAGTGGTTTGTCTGCTGAGGCTTATTGTCGCCGGCATGAAATAACTGTTATTGGTGATAATGTGCCCCCACCGTTTACTTCCTTTGAAGCTACGGGCTTCCCATCTGAGATTCTTAGAGAGGTACAAAATGCTGGTTTTTCTGCCCCGACTCCAATTCAAGCTCAATCATGGCCGGTTGCTCTTCAAAGTAGAGACATTGTGGCCATTGCTAAAACGGGGTCAGGGAAGACCTTGGGGTACTTGCTTCCTGGATTTATGCATCTTAAGCGTGCCCGTAATAACTCTCAGATGGGTCCAACTGTGTTGGTGTTATCACCAACAAGGGAGCTGGCCACACAGATTCAAGATGAAGCAATGAAGTTTGGAAAGTCTTCAAGAATAGCTGTTGTGTGTTTGTATGGAGGAGCGCCAAAGGGTCCACAATTGAGAGATATTGACAGAGGAGCAGATGTTGTGGTTGCCACTCCTGGCCGCTTGAATGACATTCTAGAAATGAAGAGAATTAGTCTCCATCAAGTTTCTTACCTAGTGTTGGATGAGGCTGATCGTATGTTGGACATGGGTTTTGAACCTCAAATAAGGAAGATTGTGAAGGAGATACCTGCTCGCCGCCAAACCTTAATGTATACGGCAACATGGCCCAAGGAGGTTAGGAAGATTGCATCAGATCTACTGGTCAACTCTGTTCAGGTGAACATTGGCAATGTTGATGAACTTGTTGCGAATAAGTCAATCACACAGCATGTTGAAGTACTGGCGTCCATGGAGAAACACAGACGATTGGAACAGATATTGCGATCGCAAGAGCCAGGGTCCAAGATAATTATATTTTGCTCAACCAAAAAGATGTGTGATCAACTTTCTCGCAACTTAACCCGCCAGTTTGGAGCTGCTGCAATTCATGGTGACAAATCTCAGACTGAGAGGGATCATGTCCTGAGTCAATTCCGCACAGGGAGGACTCCAGTTCTTGTAGCGACTGATGTTGCAGCTCGAGGATTGGATGTTAAGGACATAAGGGTGGTTATAAATTACGACTTCCCTACTGGAGTAGAGGATTATGTTCACAGGATTGGAAGAACTGGAAGGGCAGGTGCCACTGGGTTGGCTTACACATTCTTTGGTGATCAGGACGCAAAATATGCTGCAGAACTCATTAAAGTTTTGGAAGGAGCAAACCAGCGTGTACCCCCAGAAATTCGTGAGTTGGCTTCACGTGGTGGTGGGATGAACAGATTCAGACGTTGGGGCTCCGGGTCTGGAGGTCGTGATGGTGGCCGAAGTGATTCAAGCTATGGTGGAAGAGATGGTGGAAGGGGCGGTGGGTTTGGATCTTCTTTCTCTACTGGCAGATATGACCGTGGTCCAACTAATAGTTATGATGACAAGGGGTCTGCTTGGAGCAACAAAGGTTTTACTCGGGAGCGCAGTCGTAGTCCTGAAAGGTTTAACAAAGCTCCGCCAACAGCTGTTGGCTCCGGAGGTTGGAGCTTTCACAAAGCAATGATGGAACAACGGGGACGGTCATCACCGCCTCCAAATCGTGTTGAGAAGGGAGTAGTAGATGGTTTCCGTCCTTCTTTCTATGGACTGGAAGAAGCTGAGGAAGGCATGATACCACAAGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009628 GO:0009651 GO:0009987 GO:0010501 GO:0016070 GO:0016301 GO:0016310 GO:0016462 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031974 GO:0031981 GO:0034641 GO:0036211 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046777 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140096 GO:0140098 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

689

Amino Acids

74.76

Weight (kDa)

9.34

Isoelectric Point (pI)

50.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	195 - 364	5.1e-49	DEAD/DEAH box helicase
ResIII	PF04851	207 - 358	3.1e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	402 - 510	4.7e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01540 AT3G01540 AT3G01540 AT3G01540 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610 AT5G14610
fragaria_vesca	FvH4_3g33550 FvH4_6g50990
malus_domestica	MD09G1029800.v1.1 MD17G1031800.v1.1
prunus_persica	Prupe.3G287500_v2.0.a1 Prupe.3G287500_v2.0.a1
pyrus_communis	pycom17g02940
rosa_chinensis	RchiOBHm_Chr2g0172491 RchiOBHm_Chr3g0496011
rosa_laevigata	RLG00000022110 RLG00000026510
rosa_multiflora	Rmu_co8447803.1_g000001 Rmu_sc0008577.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079810 Rroxscaffold_6G00401610
rosa_rugosa	Rorug02G0559500
rosa_samantha	Rh1CG060200 Rh2AG635200 Rh2BG648700 Rh2CG615300 Rh2DG663200 Rh3AG325700 Rh5DG348900 Rh5DG552200 Rh7AG279200 Rh7CG298300
rosa_wichuraiana	Rw2G052550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1463
AarI	CACCTGC	1 cut(s)	1517
Acc36I	ACCTGC	2 cut(s)	1045, 1517
AccB1I	GGYRCC	2 cut(s)	110, 1529
AccI	GTMKAC	1 cut(s)	1061
AccIII	TCCGGA	1 cut(s)	1913
AciI	CCGC	8 cut(s)	56, 907, 1045, 1312, 1384, 1755, 1895, 1967
AclWI	GGATC	4 cut(s)	307, 406, 1371, 1774
AcoI	YGGCCR	5 cut(s)	605, 636, 765, 904, 1711
AcsI	RAATTY	2 cut(s)	305, 1632
AcvI	CACGTG	1 cut(s)	1652
AcyI	GRCGYC	2 cut(s)	111, 1193
AdeI	CACNNNGTG	1 cut(s)	1655
AfaI	GTAC	5 cut(s)	330, 558, 674, 1188, 1623
AfiI	CCNNNNNNNGG	4 cut(s)	16, 17, 967, 1920
AflII	CTTAAG	1 cut(s)	698
AgeI	ACCGGT	1 cut(s)	76
AhdI	GACNNNNNGTC	1 cut(s)	1825
AjnI	CCWGG	3 cut(s)	682, 901, 1243
Alw21I	GWGCWC	2 cut(s)	349, 391
Alw26I	GTCTC	3 cut(s)	21, 621, 947
Alw44I	GTGCAC	2 cut(s)	345, 387
AlwI	GGATC	4 cut(s)	307, 406, 1371, 1774
AlwNI	CAGNNNCTG	1 cut(s)	431
Ama87I	CYCGRG	2 cut(s)	1428, 1854
Aor13HI	TCCGGA	1 cut(s)	1913
AoxI	GGCC	7 cut(s)	164, 605, 636, 765, 904, 1073, 1711
ApaLI	GTGCAC	2 cut(s)	345, 387
ApeKI	GCWGC	6 cut(s)	141, 288, 1325, 1328, 1424, 1580
ApoI	RAATTY	2 cut(s)	305, 1632
ArsI	GACNNNNNNTTYG	2 cut(s)	983, 1015
AsiGI	ACCGGT	1 cut(s)	76
AsiSI	GCGATCGC	1 cut(s)	1235
Asp700I	GAANNNNTTC	3 cut(s)	805, 1670, 1720
AspLEI	GCGC	3 cut(s)	113, 844, 1863
AspS9I	GGNCC	8 cut(s)	317, 368, 419, 728, 851, 1074, 1248, 1799
AsuC2I	CCSGG	1 cut(s)	1690
AsuHPI	GGTGA	8 cut(s)	66, 77, 500, 741, 1138, 1352, 1571, 1956
AvaI	CYCGRG	2 cut(s)	1428, 1854
AvaII	GGWCC	7 cut(s)	317, 368, 419, 728, 851, 1248, 1799
BaeGI	GKGCMC	4 cut(s)	349, 391, 501, 709
BalI	TGGCCA	2 cut(s)	638, 767
BanI	GGYRCC	2 cut(s)	110, 1529
BanII	GRGCYC	1 cut(s)	1688
BarI	GAAGNNNNNNTAC	2 cut(s)	2034, 2066
BbrPI	CACGTG	1 cut(s)	1652
BbsI	GAAGAC	2 cut(s)	668, 798
Bbv12I	GWGCWC	2 cut(s)	349, 391
BbvCI	CCTCAGC	2 cut(s)	450, 2037
BbvI	GCAGC	6 cut(s)	128, 300, 1312, 1315, 1436, 1567
BccI	CCATC	9 cut(s)	193, 430, 544, 718, 954, 1700, 1736, 1936, 1994
BceAI	ACGGC	3 cut(s)	98, 124, 1080
BciT130I	CCWGG	3 cut(s)	684, 903, 1245
BclI	TGATCA	2 cut(s)	1288, 1561
BcnI	CCSGG	1 cut(s)	1690
BcoDI	GTCTC	3 cut(s)	21, 621, 947
BfaI	CTAG	2 cut(s)	923, 962
BfmI	CTRYAG	2 cut(s)	168, 1581
BfoI	RGCGCY	2 cut(s)	114, 845
BfrI	CTTAAG	1 cut(s)	698
BfuAI	ACCTGC	2 cut(s)	1045, 1517
BglII	AGATCT	2 cut(s)	67, 1102
BisI	GCNGC	8 cut(s)	142, 289, 907, 1045, 1326, 1329, 1425, 1581
BlsI	GCNGC	8 cut(s)	143, 290, 908, 1046, 1327, 1330, 1426, 1582
BmcAI	AGTACT	1 cut(s)	1188
Bme1390I	CCNGG	4 cut(s)	684, 903, 1245, 1690
Bme18I	GGWCC	7 cut(s)	317, 368, 419, 728, 851, 1248, 1799
BmeRI	GACNNNNNGTC	1 cut(s)	1825
BmeT110I	CYCGRG	2 cut(s)	1428, 1854
BmgT120I	GGNCC	8 cut(s)	317, 368, 419, 728, 851, 1074, 1248, 1799
BmiI	GGNNCC	7 cut(s)	112, 729, 852, 1249, 1531, 1687, 1912
BmrFI	CCNGG	4 cut(s)	684, 903, 1245, 1690
BmrI	ACTGGG	1 cut(s)	1545
BmsI	GCATC	2 cut(s)	703, 1106
BmuI	ACTGGG	1 cut(s)	1545
BoxI	GACNNNNGTC	1 cut(s)	1797
BpiI	GAAGAC	2 cut(s)	668, 798
BpmI	CTGGAG	3 cut(s)	1383, 1503, 1716
Bpu10I	CCTNAGC	2 cut(s)	450, 2037
BpuMI	CCSGG	1 cut(s)	1690
BsaAI	YACGTR	1 cut(s)	1652
BsaHI	GRCGYC	2 cut(s)	111, 1193
BsaJI	CCNNGG	8 cut(s)	15, 136, 320, 666, 1077, 1197, 1244, 1795
BsaWI	WCCGGW	2 cut(s)	76, 1913
BsaXI	ACNNNNNCTCC	6 cut(s)	166, 196, 387, 417, 870, 900
Bsc4I	CCNNNNNNNGG	4 cut(s)	16, 17, 967, 1920
Bse118I	RCCGGY	3 cut(s)	76, 465, 607
Bse1I	ACTGG	9 cut(s)	1113, 1194, 1315, 1400, 1486, 1522, 1540, 1786, 2031
Bse3DI	GCAATG	5 cut(s)	298, 639, 795, 1144, 1944
BseAI	TCCGGA	1 cut(s)	1913
BseBI	CCWGG	3 cut(s)	684, 903, 1245
BseDI	CCNNGG	8 cut(s)	15, 136, 320, 666, 1077, 1197, 1244, 1795
BseGI	GGATG	3 cut(s)	976, 1444, 1668
BseLI	CCNNNNNNNGG	4 cut(s)	16, 17, 967, 1920
BseMI	GCAATG	5 cut(s)	298, 639, 795, 1144, 1944
BseMII	CTCAG	7 cut(s)	441, 531, 734, 1347, 1364, 1364, 2028
BseNI	ACTGG	9 cut(s)	1113, 1194, 1315, 1400, 1486, 1522, 1540, 1786, 2031
BseRI	GAGGAG	3 cut(s)	171, 852, 891
BseSI	GKGCMC	4 cut(s)	349, 391, 501, 709
BseXI	GCAGC	6 cut(s)	128, 300, 1312, 1315, 1436, 1567
BsgI	GTGCAG	1 cut(s)	186
Bsh1285I	CGRYCG	1 cut(s)	1235
BshFI	GGCC	7 cut(s)	166, 607, 638, 767, 906, 1075, 1713
BshNI	GGYRCC	2 cut(s)	110, 1529
BshTI	ACCGGT	1 cut(s)	76
BsiEI	CGRYCG	1 cut(s)	1235
BsiHKAI	GWGCWC	2 cut(s)	349, 391
BsiHKCI	CYCGRG	2 cut(s)	1428, 1854
BsiSI	CCGG	5 cut(s)	77, 466, 608, 1689, 1914
BslFI	GGGAC	1 cut(s)	1968
BslI	CCNNNNNNNGG	4 cut(s)	16, 17, 967, 1920
BsmAI	GTCTC	3 cut(s)	21, 621, 947
BsmFI	GGGAC	1 cut(s)	1968
BsnI	GGCC	7 cut(s)	166, 607, 638, 767, 906, 1075, 1713
BsoBI	CYCGRG	2 cut(s)	1428, 1854
Bsp1286I	GDGCHC	5 cut(s)	349, 391, 501, 709, 1688
Bsp13I	TCCGGA	1 cut(s)	1913
Bsp1407I	TGTACA	1 cut(s)	328
Bsp19I	CCATGG	2 cut(s)	136, 1197
BspACI	CCGC	8 cut(s)	56, 907, 1045, 1312, 1384, 1755, 1895, 1967
BspANI	GGCC	7 cut(s)	166, 607, 638, 767, 906, 1075, 1713
BspCNI	CTCAG	7 cut(s)	442, 532, 733, 1348, 1363, 1365, 2029
BspEI	TCCGGA	1 cut(s)	1913
BspLI	GGNNCC	7 cut(s)	112, 729, 852, 1249, 1531, 1687, 1912
BspMAI	CTGCAG	1 cut(s)	1585
BspMI	ACCTGC	2 cut(s)	1045, 1517
BspPI	GGATC	4 cut(s)	307, 406, 1371, 1774
BspQI	GCTCTTC	1 cut(s)	621
BspT107I	GGYRCC	2 cut(s)	110, 1529
BspTI	CTTAAG	1 cut(s)	698
BsrDI	GCAATG	5 cut(s)	298, 639, 795, 1144, 1944
BsrFI	RCCGGY	3 cut(s)	76, 465, 607
BsrGI	TGTACA	1 cut(s)	328
BsrI	ACTGG	9 cut(s)	1113, 1194, 1315, 1400, 1486, 1522, 1540, 1786, 2031
BssAI	RCCGGY	3 cut(s)	76, 465, 607
BssECI	CCNNGG	8 cut(s)	15, 136, 320, 666, 1077, 1197, 1244, 1795
BssNAI	GTATAC	1 cut(s)	1062
BssNI	GRCGYC	2 cut(s)	111, 1193
BssT1I	CCWWGG	5 cut(s)	15, 136, 666, 1077, 1197
Bst1107I	GTATAC	1 cut(s)	1062
Bst2UI	CCWGG	3 cut(s)	684, 903, 1245
Bst4CI	ACNGT	6 cut(s)	207, 481, 507, 736, 1796, 1959
Bst6I	CTCTTC	3 cut(s)	621, 926, 1732
BstACI	GRCGYC	2 cut(s)	111, 1193
BstAFI	CTTAAG	1 cut(s)	698
BstAPI	GCANNNNNTGC	2 cut(s)	344, 1577
BstAUI	TGTACA	1 cut(s)	328
BstBAI	YACGTR	1 cut(s)	1652
BstC8I	GCNNGC	5 cut(s)	164, 467, 705, 765, 1042
BstDEI	CTNAG	8 cut(s)	450, 540, 548, 720, 1350, 1356, 1373, 2037
BstDSI	CCRYGG	3 cut(s)	136, 1197, 1795
BstF5I	GGATG	3 cut(s)	976, 1444, 1668
BstH2I	RGCGCY	2 cut(s)	114, 845
BstHHI	GCGC	3 cut(s)	113, 844, 1863
BstMAI	GTCTC	3 cut(s)	21, 621, 947
BstMCI	CGRYCG	1 cut(s)	1235
BstMWI	GCNNNNNNNGC	7 cut(s)	141, 147, 200, 288, 344, 700, 1577
BstNI	CCWGG	3 cut(s)	684, 903, 1245
BstNSI	RCATGY	1 cut(s)	1181
BstPAI	GACNNNNGTC	1 cut(s)	1797
BstSCI	CCNGG	4 cut(s)	682, 901, 1243, 1688
BstSFI	CTRYAG	2 cut(s)	168, 1581
BstSLI	GKGCMC	4 cut(s)	349, 391, 501, 709
BstV1I	GCAGC	6 cut(s)	128, 300, 1312, 1315, 1436, 1567
BstV2I	GAAGAC	2 cut(s)	668, 798
BstX2I	RGATCY	4 cut(s)	67, 398, 1102, 1766
BstXI	CCANNNNNNTGG	1 cut(s)	1540
BstYI	RGATCY	4 cut(s)	67, 398, 1102, 1766
BstZ17I	GTATAC	1 cut(s)	1062
BsuRI	GGCC	7 cut(s)	166, 607, 638, 767, 906, 1075, 1713
BtgI	CCRYGG	3 cut(s)	136, 1197, 1795
BtsCI	GGATG	3 cut(s)	976, 1444, 1668
BtsI	GCAGTG	1 cut(s)	145
BtsIMutI	CAGTG	2 cut(s)	145, 1533
BveI	ACCTGC	2 cut(s)	1045, 1517
Cac8I	GCNNGC	5 cut(s)	164, 467, 705, 765, 1042
CaiI	CAGNNNCTG	1 cut(s)	431
CfoI	GCGC	3 cut(s)	113, 844, 1863
Cfr10I	RCCGGY	3 cut(s)	76, 465, 607
Cfr13I	GGNCC	8 cut(s)	317, 368, 419, 728, 851, 1074, 1248, 1799
CseI	GACGC	2 cut(s)	1182, 1577
Csp6I	GTAC	5 cut(s)	329, 557, 673, 1187, 1622
CspAI	ACCGGT	1 cut(s)	76
CviQI	GTAC	5 cut(s)	329, 557, 673, 1187, 1622
DdeI	CTNAG	8 cut(s)	450, 540, 548, 720, 1350, 1356, 1373, 2037
DinI	GGCGCC	1 cut(s)	112
DraIII	CACNNNGTG	1 cut(s)	1655
DriI	GACNNNNNGTC	1 cut(s)	1825
EaeI	YGGCCR	5 cut(s)	605, 636, 765, 904, 1711
Eam1104I	CTCTTC	3 cut(s)	621, 926, 1732
Eam1105I	GACNNNNNGTC	1 cut(s)	1825
EarI	CTCTTC	3 cut(s)	621, 926, 1732
EciI	GGCGGA	1 cut(s)	1884
Eco130I	CCWWGG	5 cut(s)	15, 136, 666, 1077, 1197
Eco24I	GRGCYC	1 cut(s)	1688
Eco47I	GGWCC	7 cut(s)	317, 368, 419, 728, 851, 1248, 1799
Eco72I	CACGTG	1 cut(s)	1652
Eco88I	CYCGRG	2 cut(s)	1428, 1854
EcoRII	CCWGG	3 cut(s)	682, 901, 1243
EcoT14I	CCWWGG	5 cut(s)	15, 136, 666, 1077, 1197
EcoT22I	ATGCAT	1 cut(s)	696
EcoT38I	GRGCYC	1 cut(s)	1688
EgeI	GGCGCC	1 cut(s)	112
EheI	GGCGCC	1 cut(s)	112
ErhI	CCWWGG	5 cut(s)	15, 136, 666, 1077, 1197
FaqI	GGGAC	1 cut(s)	1968
FauI	CCCGC	2 cut(s)	63, 1319
FbaI	TGATCA	2 cut(s)	1288, 1561
FblI	GTMKAC	1 cut(s)	1061
Fnu4HI	GCNGC	8 cut(s)	142, 289, 907, 1045, 1326, 1329, 1425, 1581
FokI	GGATG	3 cut(s)	983, 1451, 1675
FriOI	GRGCYC	1 cut(s)	1688
Fsp4HI	GCNGC	8 cut(s)	142, 289, 907, 1045, 1326, 1329, 1425, 1581
FspBI	CTAG	2 cut(s)	923, 962
GlaI	GCGC	3 cut(s)	112, 843, 1862
GluI	GCNGC	8 cut(s)	142, 289, 907, 1045, 1326, 1329, 1425, 1581
GsuI	CTGGAG	3 cut(s)	1383, 1503, 1716
HaeII	RGCGCY	2 cut(s)	114, 845
HaeIII	GGCC	7 cut(s)	166, 607, 638, 767, 906, 1075, 1713
HapII	CCGG	5 cut(s)	77, 466, 608, 1689, 1914
HgaI	GACGC	2 cut(s)	1182, 1577
HhaI	GCGC	3 cut(s)	113, 844, 1863
Hin1I	GRCGYC	2 cut(s)	111, 1193
Hin6I	GCGC	3 cut(s)	111, 842, 1861
HinP1I	GCGC	3 cut(s)	111, 842, 1861
HincII	GTYRAC	1 cut(s)	1114
HindII	GTYRAC	1 cut(s)	1114
HinfI	GANTC	8 cut(s)	428, 544, 583, 775, 1375, 1396, 1671, 1721
HpaII	CCGG	5 cut(s)	77, 466, 608, 1689, 1914
HphI	GGTGA	8 cut(s)	66, 77, 500, 741, 1138, 1352, 1571, 1956
Hpy188I	TCNGA	7 cut(s)	67, 427, 541, 723, 1102, 1353, 1676
Hpy99I	CGWCG	1 cut(s)	258
HpyAV	CCTTC	8 cut(s)	98, 1021, 1515, 1598, 1743, 1981, 2022, 2036
HpyCH4III	ACNGT	6 cut(s)	207, 481, 507, 736, 1796, 1959
HpyCH4IV	ACGT	3 cut(s)	253, 1651, 1678
HpyF10VI	GCNNNNNNNGC	7 cut(s)	141, 147, 200, 288, 344, 700, 1577
HpyF3I	CTNAG	8 cut(s)	450, 540, 548, 720, 1350, 1356, 1373, 2037
HpySE526I	ACGT	3 cut(s)	253, 1651, 1678
Hsp92I	GRCGYC	2 cut(s)	111, 1193
HspAI	GCGC	3 cut(s)	111, 842, 1861
KasI	GGCGCC	1 cut(s)	110
Kpn2I	TCCGGA	1 cut(s)	1913
KroI	GCCGGC	1 cut(s)	465
KroNI	GCCGGC	1 cut(s)	467
Ksp22I	TGATCA	2 cut(s)	1288, 1561
LguI	GCTCTTC	1 cut(s)	621
Lsp1109I	GCAGC	6 cut(s)	128, 300, 1312, 1315, 1436, 1567
LweI	GCATC	2 cut(s)	703, 1106
MaeI	CTAG	2 cut(s)	923, 962
MaeII	ACGT	3 cut(s)	253, 1651, 1678
MaeIII	GTNAC	1 cut(s)	1340
MfeI	CAATTG	1 cut(s)	857
MflI	RGATCY	4 cut(s)	67, 398, 1102, 1766
MhlI	GDGCHC	5 cut(s)	349, 391, 501, 709, 1688
MlsI	TGGCCA	2 cut(s)	638, 767
MluNI	TGGCCA	2 cut(s)	638, 767
Mly113I	GGCGCC	1 cut(s)	111
MlyI	GAGTC	4 cut(s)	422, 577, 1384, 1390
MmeI	TCCRAC	5 cut(s)	755, 948, 969, 1826, 1901
Mox20I	TGGCCA	2 cut(s)	638, 767
Mph1103I	ATGCAT	1 cut(s)	696
MroI	TCCGGA	1 cut(s)	1913
MroNI	GCCGGC	1 cut(s)	465
MroXI	GAANNNNTTC	3 cut(s)	805, 1670, 1720
MscI	TGGCCA	2 cut(s)	638, 767
MseI	TTAA	7 cut(s)	129, 699, 1055, 1307, 1443, 1593, 1884
MslI	CAYNNNNRTG	1 cut(s)	1454
Msp20I	TGGCCA	2 cut(s)	638, 767
MspA1I	CMGCKG	1 cut(s)	1904
MspCI	CTTAAG	1 cut(s)	698
MspI	CCGG	5 cut(s)	77, 466, 608, 1689, 1914
MspR9I	CCNGG	4 cut(s)	684, 903, 1245, 1690
MunI	CAATTG	1 cut(s)	857
MvaI	CCWGG	3 cut(s)	684, 903, 1245
MwoI	GCNNNNNNNGC	7 cut(s)	141, 147, 200, 288, 344, 700, 1577
NaeI	GCCGGC	1 cut(s)	467
NarI	GGCGCC	1 cut(s)	111
NciI	CCSGG	1 cut(s)	1690
NcoI	CCATGG	2 cut(s)	136, 1197
NgoMIV	GCCGGC	1 cut(s)	465
NlaIV	GGNNCC	7 cut(s)	112, 729, 852, 1249, 1531, 1687, 1912
NmuCI	GTSAC	1 cut(s)	1340
NsiI	ATGCAT	1 cut(s)	696
NspI	RCATGY	1 cut(s)	1181
PaeR7I	CTCGAG	1 cut(s)	1428
PaqCI	CACCTGC	1 cut(s)	1517
PciSI	GCTCTTC	1 cut(s)	621
PdiI	GCCGGC	1 cut(s)	467
PdmI	GAANNNNTTC	3 cut(s)	805, 1670, 1720
PfeI	GAWTC	4 cut(s)	544, 775, 1671, 1721
PflFI	GACNNNGTC	1 cut(s)	252
PfoI	TCCNGGA	1 cut(s)	682
PinAI	ACCGGT	1 cut(s)	76
PkrI	GCNGC	8 cut(s)	143, 290, 908, 1046, 1327, 1330, 1426, 1582
Ple19I	CGATCG	1 cut(s)	1235
PleI	GAGTC	4 cut(s)	422, 577, 1383, 1390
PluTI	GGCGCC	1 cut(s)	114
PmaCI	CACGTG	1 cut(s)	1652
PmlI	CACGTG	1 cut(s)	1652
PpsI	GAGTC	4 cut(s)	422, 577, 1383, 1390
Ppu21I	YACGTR	1 cut(s)	1652
PshAI	GACNNNNGTC	1 cut(s)	1797
PsiI	TTATAA	1 cut(s)	1463
Psp6I	CCWGG	3 cut(s)	682, 901, 1243
PspCI	CACGTG	1 cut(s)	1652
PspGI	CCWGG	3 cut(s)	682, 901, 1243
PspN4I	GGNNCC	7 cut(s)	112, 729, 852, 1249, 1531, 1687, 1912
PspPI	GGNCC	8 cut(s)	317, 368, 419, 728, 851, 1074, 1248, 1799
PspXI	VCTCGAGB	1 cut(s)	1428
PstI	CTGCAG	1 cut(s)	1585
PstNI	CAGNNNCTG	1 cut(s)	431
PsuI	RGATCY	4 cut(s)	67, 398, 1102, 1766
PsyI	GACNNNGTC	1 cut(s)	252
PvuI	CGATCG	1 cut(s)	1235
PvuII	CAGCTG	1 cut(s)	1904
RgaI	GCGATCGC	1 cut(s)	1235
RsaI	GTAC	5 cut(s)	330, 558, 674, 1188, 1623
RsaNI	GTAC	5 cut(s)	329, 557, 673, 1187, 1622
RseI	CAYNNNNRTG	1 cut(s)	1454
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	7 cut(s)	129, 699, 1055, 1307, 1443, 1593, 1884
SatI	GCNGC	8 cut(s)	142, 289, 907, 1045, 1326, 1329, 1425, 1581
Sau96I	GGNCC	8 cut(s)	317, 368, 419, 728, 851, 1074, 1248, 1799
ScaI	AGTACT	1 cut(s)	1188
SchI	GAGTC	4 cut(s)	422, 577, 1384, 1390
ScrFI	CCNGG	4 cut(s)	684, 903, 1245, 1690
SduI	GDGCHC	5 cut(s)	349, 391, 501, 709, 1688
SfaAI	GCGATCGC	1 cut(s)	1235
SfaNI	GCATC	2 cut(s)	703, 1106
SfcI	CTRYAG	2 cut(s)	168, 1581
SfoI	GGCGCC	1 cut(s)	112
Sfr274I	CTCGAG	1 cut(s)	1428
SgfI	GCGATCGC	1 cut(s)	1235
SgrAI	CRCCGGYG	1 cut(s)	76
SinI	GGWCC	7 cut(s)	317, 368, 419, 728, 851, 1248, 1799
SlaI	CTCGAG	1 cut(s)	1428
SmiMI	CAYNNNNRTG	1 cut(s)	1454
SmlI	CTYRAG	2 cut(s)	698, 1428
SmoI	CTYRAG	2 cut(s)	698, 1428
SsiI	CCGC	8 cut(s)	56, 907, 1045, 1312, 1384, 1755, 1895, 1967
SspDI	GGCGCC	1 cut(s)	110
SspMI	CTAG	2 cut(s)	923, 962
StyD4I	CCNGG	4 cut(s)	682, 901, 1243, 1688
StyI	CCWWGG	5 cut(s)	15, 136, 666, 1077, 1197
TaaI	ACNGT	6 cut(s)	207, 481, 507, 736, 1796, 1959
TaiI	ACGT	3 cut(s)	256, 1654, 1681
TaqI	TCGA	1 cut(s)	1429
TaqII	GACCGA	1 cut(s)	334
TatI	WGTACW	2 cut(s)	328, 1186
TauI	GCSGC	2 cut(s)	909, 1047
TfiI	GAWTC	4 cut(s)	544, 775, 1671, 1721
Tru1I	TTAA	7 cut(s)	129, 699, 1055, 1307, 1443, 1593, 1884
Tru9I	TTAA	7 cut(s)	129, 699, 1055, 1307, 1443, 1593, 1884
TscAI	CASTG	2 cut(s)	152, 1540
TseFI	GTSAC	1 cut(s)	1340
TseI	GCWGC	6 cut(s)	141, 288, 1325, 1328, 1424, 1580
Tsp45I	GTSAC	1 cut(s)	1340
TspDTI	ATGAA	8 cut(s)	285, 486, 798, 806, 944, 1161, 1325, 1679
TspGWI	ACGGA	2 cut(s)	81, 1997
TspRI	CASTG	2 cut(s)	152, 1540
Tth111I	GACNNNGTC	1 cut(s)	252
Vha464I	CTTAAG	1 cut(s)	698
VneI	GTGCAC	2 cut(s)	345, 387
VpaK11BI	GGWCC	7 cut(s)	317, 368, 419, 728, 851, 1248, 1799
XapI	RAATTY	2 cut(s)	305, 1632
XbaI	TCTAGA	1 cut(s)	922
XceI	RCATGY	1 cut(s)	1181
XhoI	CTCGAG	1 cut(s)	1428
XmiI	GTMKAC	1 cut(s)	1061
XmnI	GAANNNNTTC	3 cut(s)	805, 1670, 1720
XspI	CTAG	2 cut(s)	923, 962
ZrmI	AGTACT	1 cut(s)	1188
Zsp2I	ATGCAT	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.