FvH4_7g09530

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005075
Physical Location & Seq
Reverse (-)
9137600 .. 9142342
4743 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09530.t2

Sequence Viewer

Length: 936 bp
ATGGTTCTGTCACAGAGGCTTCACGAGGCCTTCAAAGGCACGGTCGAGAAGATCACAGGCCCCCGCACCGTCTCCGCTTTCAAAGAGAAAGGCGTTCTCAGCGTCTCCGAGTTCGTCACCGCCGGCGATAACCTCGTCTCCAAGTGCCCTACCTGGTCCTGGGAATCAGGGGAGCCGAGCAAGAGGAAGCCATACCTACCGTCTGAGAAGCAGTATTTGATTACTAGAAATGTCCCTTGTTTACGGAGGGCTGCTAGTGTGGAAGAGGAGTATGAGGCTGCAGGCATGGAAGTTCTTATCGATAATGAAGATAATGATGGCTGGTTGGCAACACACGGGAAGCCAAAGGAGCCAAAGGATAGAAGTAGCGAGGAGGAAAACTTTCCTTCCATTGACAGTTTAGAAATCAGCAAGGATAACTCTGTGAACTCAGCCCCCTCCCAAGGTGGGGCGGAAGATGAAGACGATATCCCTGACATGGCTGATTTTGAAGAAGCTGACAACATTGAAATAGATCCGGGAACATATCTTGTTGCTCATGAACCTGATGACGACAATATTTTACGGACCCGAACATATGATGTCAGCATTACGTATGATAAATATTACCAAACTCCTCGGGTGTGGCTTACTGGCTATGATGAGTCAAGGATGCTTTTACAAGCAGAGCTTGTACTTGAAGATGTCAGTCAAGATCATGCACGCAAAACTGTAACTATTGAGGATCATCCTCACTTGCCCGGGAAGCATGCATCTGTCCATCCCTGCCGACATGGAGCTGTGATGAAGAAAATAATTGATGTACTAATGTCACGTGGAGTGGAACCAGAAGTTGATAAGTACCTTTTCTTGTTCTTGAAATTCGTTGCCTCTGTGATTCCAACCATCGAATATGACTATACAATGGACTTTGATCTGGGCAGTTCCAGCAGTTGA

Protein Analysis

312

Amino Acids

35.02

Weight (kDa)

4.66

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 37 - 300 6.1e-60 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 64, 75, 120, 452
AclWI GGATC 2 cut(s) 509, 732
AcsI RAATTY 1 cut(s) 860
AcvI CACGTG 1 cut(s) 815
AfaI GTAC 3 cut(s) 675, 804, 842
AfiI CCNNNNNNNGG 5 cut(s) 159, 443, 447, 448, 478
AgsI TTSAA 6 cut(s) 34, 82, 491, 509, 680, 859
AjnI CCWGG 2 cut(s) 152, 158
AluBI AGCT 3 cut(s) 497, 670, 779
AluI AGCT 3 cut(s) 497, 670, 779
Alw26I GTCTC 3 cut(s) 76, 109, 142
AlwI GGATC 2 cut(s) 509, 732
Ama87I CYCGRG 2 cut(s) 618, 740
AoxI GGCC 2 cut(s) 27, 58
ApeKI GCWGC 2 cut(s) 251, 278
ApoI RAATTY 1 cut(s) 860
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 381
AspS9I GGNCC 3 cut(s) 59, 156, 567
AsuC2I CCSGG 3 cut(s) 519, 741, 742
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 2 cut(s) 618, 740
AvaII GGWCC 2 cut(s) 156, 567
BaeGI GKGCMC 1 cut(s) 149
BauI CACGAG 1 cut(s) 23
BbrPI CACGTG 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 238, 265
BccI CCATC 3 cut(s) 311, 768, 893
BciT130I CCWGG 2 cut(s) 154, 160
BcnI CCSGG 3 cut(s) 519, 741, 742
BcoDI GTCTC 3 cut(s) 76, 109, 142
BfaI CTAG 2 cut(s) 225, 255
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 2 cut(s) 252, 279
BlsI GCNGC 2 cut(s) 253, 280
Bme1390I CCNGG 5 cut(s) 154, 160, 519, 741, 742
Bme18I GGWCC 2 cut(s) 156, 567
BmeT110I CYCGRG 2 cut(s) 618, 740
BmgT120I GGNCC 3 cut(s) 59, 156, 567
BmiI GGNNCC 5 cut(s) 61, 174, 351, 569, 825
BmrFI CCNGG 5 cut(s) 154, 160, 519, 741, 742
BmsI GCATC 2 cut(s) 642, 761
BpiI GAAGAC 1 cut(s) 468
BpuMI CCSGG 3 cut(s) 519, 741, 742
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 2 cut(s) 594, 815
BsaJI CCNNGG 4 cut(s) 159, 442, 617, 740
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 443, 447, 448, 478
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 637
BseBI CCWGG 2 cut(s) 154, 160
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 4 cut(s) 159, 442, 617, 740
BseGI GGATG 3 cut(s) 657, 727, 760
BseLI CCNNNNNNNGG 5 cut(s) 159, 443, 447, 448, 478
BseMII CTCAG 3 cut(s) 112, 195, 444
BseNI ACTGG 1 cut(s) 637
BseRI GAGGAG 3 cut(s) 281, 386, 606
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 2 cut(s) 238, 265
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 29, 60
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 2 cut(s) 618, 740
BsiSI CCGG 3 cut(s) 123, 518, 741
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 5 cut(s) 159, 443, 447, 448, 478
BsmAI GTCTC 3 cut(s) 76, 109, 142
BsmBI CGTCTC 3 cut(s) 76, 109, 142
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 2 cut(s) 29, 60
BsoBI CYCGRG 2 cut(s) 618, 740
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 5 cut(s) 51, 514, 694, 724, 913
BspACI CCGC 4 cut(s) 64, 75, 120, 452
BspANI GGCC 2 cut(s) 29, 60
BspCNI CTCAG 3 cut(s) 111, 196, 443
BspDI ATCGAT 1 cut(s) 300
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 5 cut(s) 61, 174, 351, 569, 825
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 509, 732
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 637
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 159, 442, 617, 740
BssMI GATC 5 cut(s) 51, 514, 694, 724, 913
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 442
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 154, 160
Bst4CI ACNGT 5 cut(s) 43, 70, 201, 398, 712
Bst6I CTCTTC 1 cut(s) 258
BstBAI YACGTR 2 cut(s) 594, 815
BstC8I GCNNGC 4 cut(s) 124, 283, 703, 750
BstDEI CTNAG 3 cut(s) 98, 204, 430
BstF5I GGATG 3 cut(s) 657, 727, 760
BstKTI GATC 5 cut(s) 54, 517, 697, 727, 916
BstMAI GTCTC 3 cut(s) 76, 109, 142
BstMBI GATC 5 cut(s) 51, 514, 694, 724, 913
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 99, 349, 745, 927
BstNI CCWGG 2 cut(s) 154, 160
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 5 cut(s) 152, 158, 517, 739, 740
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 594
BstV1I GCAGC 2 cut(s) 238, 265
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 2 cut(s) 29, 60
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 3 cut(s) 657, 727, 760
Cac8I GCNNGC 4 cut(s) 124, 283, 703, 750
CciI TCATGA 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 59, 156, 567
Cfr9I CCCGGG 1 cut(s) 740
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 3 cut(s) 674, 803, 841
CviAII CATG 6 cut(s) 286, 478, 539, 698, 749, 773
CviQI GTAC 3 cut(s) 674, 803, 841
DdeI CTNAG 3 cut(s) 98, 204, 430
DpnI GATC 5 cut(s) 53, 516, 696, 726, 915
DpnII GATC 5 cut(s) 51, 514, 694, 724, 913
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 467
Eco105I TACGTA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 442
Eco147I AGGCCT 1 cut(s) 29
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 2 cut(s) 156, 567
Eco72I CACGTG 1 cut(s) 815
Eco88I CYCGRG 2 cut(s) 618, 740
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 152, 158
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 754
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 3 cut(s) 76, 109, 142
FaeI CATG 6 cut(s) 289, 481, 542, 701, 752, 776
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 285, 477, 538, 697, 748, 772
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 252, 279
FokI GGATG 3 cut(s) 664, 714, 747
Fsp4HI GCNGC 2 cut(s) 252, 279
FspBI CTAG 2 cut(s) 225, 255
GluI GCNGC 2 cut(s) 252, 279
HaeIII GGCC 2 cut(s) 29, 60
HapII CCGG 3 cut(s) 123, 518, 741
HgaI GACGC 1 cut(s) 91
Hin1II CATG 6 cut(s) 289, 481, 542, 701, 752, 776
HinfI GANTC 3 cut(s) 164, 644, 877
HpaII CCGG 3 cut(s) 123, 518, 741
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 242, 427
Hpy188I TCNGA 2 cut(s) 109, 205
Hpy188III TCNNGA 5 cut(s) 23, 46, 539, 692, 856
Hpy8I GTNNAC 2 cut(s) 242, 427
HpyAV CCTTC 2 cut(s) 40, 396
HpyCH4III ACNGT 5 cut(s) 43, 70, 201, 398, 712
HpyCH4IV ACGT 2 cut(s) 593, 814
HpyCH4V TGCA 3 cut(s) 281, 701, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 349, 745, 927
HpyF3I CTNAG 3 cut(s) 98, 204, 430
HpySE526I ACGT 2 cut(s) 593, 814
Hsp92II CATG 6 cut(s) 289, 481, 542, 701, 752, 776
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 5 cut(s) 51, 514, 694, 724, 913
LmnI GCTCC 3 cut(s) 172, 349, 776
Lsp1109I GCAGC 2 cut(s) 238, 265
LweI GCATC 2 cut(s) 642, 761
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 2 cut(s) 225, 255
MaeII ACGT 2 cut(s) 593, 814
MaeIII GTNAC 4 cut(s) 9, 115, 712, 810
MalI GATC 5 cut(s) 53, 516, 696, 726, 915
MboI GATC 5 cut(s) 51, 514, 694, 724, 913
MboII GAAGA 8 cut(s) 61, 275, 320, 467, 473, 503, 692, 799
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 2 cut(s) 795, 860
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 905
Mph1103I ATGCAT 1 cut(s) 754
MreI CGCCGGCG 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 381
MspI CCGG 3 cut(s) 123, 518, 741
MspR9I CCNGG 5 cut(s) 154, 160, 519, 741, 742
MvaI CCWGG 2 cut(s) 154, 160
MwoI GCNNNNNNNGC 4 cut(s) 99, 349, 745, 927
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 3 cut(s) 519, 741, 742
NdeI CATATG 1 cut(s) 577
NdeII GATC 5 cut(s) 51, 514, 694, 724, 913
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 6 cut(s) 289, 481, 542, 701, 752, 776
NlaIV GGNNCC 5 cut(s) 61, 174, 351, 569, 825
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 3 cut(s) 9, 115, 810
NsiI ATGCAT 1 cut(s) 754
NspI RCATGY 1 cut(s) 752
PaeI GCATGC 1 cut(s) 752
PagI TCATGA 1 cut(s) 538
PceI AGGCCT 1 cut(s) 29
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 164, 877
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 2 cut(s) 253, 280
PleI GAGTC 1 cut(s) 652
PmaCI CACGTG 1 cut(s) 815
PmlI CACGTG 1 cut(s) 815
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 2 cut(s) 594, 815
Psp6I CCWGG 2 cut(s) 152, 158
PspCI CACGTG 1 cut(s) 815
PspGI CCWGG 2 cut(s) 152, 158
PspN4I GGNNCC 5 cut(s) 61, 174, 351, 569, 825
PspPI GGNCC 3 cut(s) 59, 156, 567
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 3 cut(s) 675, 804, 842
RsaNI GTAC 3 cut(s) 674, 803, 841
SatI GCNGC 2 cut(s) 252, 279
Sau3AI GATC 5 cut(s) 51, 514, 694, 724, 913
Sau96I GGNCC 3 cut(s) 59, 156, 567
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 5 cut(s) 154, 160, 519, 741, 742
SduI GDGCHC 1 cut(s) 149
SexAI ACCWGGT 1 cut(s) 152
SfaNI GCATC 2 cut(s) 642, 761
SfcI CTRYAG 1 cut(s) 279
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 156, 567
SmaI CCCGGG 1 cut(s) 742
SnaBI TACGTA 1 cut(s) 594
SphI GCATGC 1 cut(s) 752
Sse9I AATT 2 cut(s) 795, 860
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 4 cut(s) 64, 75, 120, 452
SspI AATATT 2 cut(s) 559, 605
SspMI CTAG 2 cut(s) 225, 255
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 5 cut(s) 152, 158, 517, 739, 740
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 5 cut(s) 43, 70, 201, 398, 712
TaiI ACGT 2 cut(s) 596, 817
TaqI TCGA 3 cut(s) 45, 300, 888
TasI AATT 2 cut(s) 795, 860
TatI WGTACW 2 cut(s) 673, 802
TfiI GAWTC 2 cut(s) 164, 877
TseFI GTSAC 3 cut(s) 9, 115, 810
TseI GCWGC 2 cut(s) 251, 278
Tsp45I GTSAC 3 cut(s) 9, 115, 810
TspDTI ATGAA 4 cut(s) 321, 474, 555, 800
TspGWI ACGGA 2 cut(s) 259, 580
TspMI CCCGGG 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 156, 567
XapI RAATTY 1 cut(s) 860
XceI RCATGY 1 cut(s) 752
XmaI CCCGGG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 2 cut(s) 225, 255
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.