Rh1AG198600

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005075
Physical Location & Seq
Reverse (-)
37789088 .. 37794909
5822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG198600.1

Sequence Viewer

Length: 759 bp
ATGGTTCTGTCACAGAGGCTTCACGAGGCGTTCAAAGGCACCGTCGAGAAAATCACAGGGCCTCGCACCGTCTCCGCTTTCAAAGAGAAAGGCGTCCTCAGCGTCTCCGAGTTCGTCACCGCCGGCGATAACCTCGTCTCCAAGTGCCCTACCTGGTCTTGGGAATCCGGAGAGCCAAGCAAGAGGAAGCCATACCTACCATCTGAAAAGCAGTATTTGATTACTAGAAATGTCCCTTGTTTACGCAGGGCTGCTTGTGTGGAAGAGGAGTACGAAGCTGCAGGTTCGGAAGTTCTTATCGATAATGAAGATAATGATGGCTGGTTGGCAACACACGGGAAGCCAAAGGATAAAAGTAGTGAGGAGGAAAACTTTCCTTCCATTGAAAGTTTAGAAATCAGCAAGGTGAGGTCGGCCCCCTCGCAAGTTGGGGGTGAAGATGAAGATGATATCCCTGACATGGCTGATTATGAAGAAGCTGACAACATTGAAATAGACCCTGGAACATATCTCGTTGCTCATGAACCTGATGATGACAATATTTTACGGACCCGGACATATGATGTCAGCATTACGTATGATAAATATTACCAAACTCCTCGGGTGTGGCTTACTGGCTATGATGAGGTTTGTCCCTGCAATTTTATCTATTCCTTTTGCAAGTGTATTATTATCAATAAATGCTGTCAATTTGGTATTCAACTGAATCAGCTGCATGTGTTTTTTTTTTGGGTCAAAGAGCTGCATGTTTGGTCATGA

Protein Analysis

252

Amino Acids

28.76

Weight (kDa)

4.67

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 37 - 209 3.2e-27 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 2 cut(s) 75, 120
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 159, 460
AgsI TTSAA 5 cut(s) 34, 82, 386, 491, 701
AjnI CCWGG 2 cut(s) 152, 499
AluBI AGCT 4 cut(s) 278, 479, 712, 742
AluI AGCT 4 cut(s) 278, 479, 712, 742
Alw26I GTCTC 3 cut(s) 76, 109, 142
Ama87I CYCGRG 1 cut(s) 600
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 2 cut(s) 59, 414
ApeKI GCWGC 4 cut(s) 251, 278, 712, 742
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 59, 415, 549
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 3 cut(s) 109, 418, 446
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 1 cut(s) 549
BaeGI GKGCMC 1 cut(s) 149
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 238, 265, 699, 729
BccI CCATC 2 cut(s) 208, 311
BciT130I CCWGG 2 cut(s) 154, 501
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 3 cut(s) 76, 109, 142
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 4 cut(s) 252, 279, 713, 743
BlsI GCNGC 4 cut(s) 253, 280, 714, 744
Bme1390I CCNGG 3 cut(s) 154, 501, 553
Bme18I GGWCC 1 cut(s) 549
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 3 cut(s) 59, 415, 549
BmiI GGNNCC 3 cut(s) 40, 417, 551
BmrFI CCNGG 3 cut(s) 154, 501, 553
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 553
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 499, 599
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 460
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 619
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 2 cut(s) 154, 501
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 499, 599
BseLI CCNNNNNNNGG 2 cut(s) 159, 460
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 3 cut(s) 281, 377, 588
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 4 cut(s) 238, 265, 699, 729
BshFI GGCC 2 cut(s) 61, 416
BshNI GGYRCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 3 cut(s) 123, 168, 553
BslFI GGGAC 2 cut(s) 218, 618
BslI CCNNNNNNNGG 2 cut(s) 159, 460
BsmAI GTCTC 3 cut(s) 76, 109, 142
BsmBI CGTCTC 3 cut(s) 76, 109, 142
BsmFI GGGAC 2 cut(s) 218, 618
BsnI GGCC 2 cut(s) 61, 416
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 167
BspACI CCGC 2 cut(s) 75, 120
BspANI GGCC 2 cut(s) 61, 416
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 300
BspEI TCCGGA 1 cut(s) 167
BspHI TCATGA 2 cut(s) 520, 755
BspLI GGNNCC 3 cut(s) 40, 417, 551
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 619
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 499, 599
BssNI GRCGYC 1 cut(s) 93
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 154, 501
Bst4CI ACNGT 2 cut(s) 43, 70
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 576
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 98
BstMAI GTCTC 3 cut(s) 76, 109, 142
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNI CCWGG 2 cut(s) 154, 501
BstNSI RCATGY 2 cut(s) 719, 749
BstSCI CCNGG 3 cut(s) 152, 499, 551
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 576
BstV1I GCAGC 4 cut(s) 238, 265, 699, 729
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 2 cut(s) 61, 416
BsuTUI ATCGAT 1 cut(s) 300
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 124
CciI TCATGA 2 cut(s) 520, 755
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 59, 415, 549
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 2 cut(s) 82, 91
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 1 cut(s) 271
CviAII CATG 5 cut(s) 460, 521, 716, 746, 756
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 576
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 152, 499
EcoRV GATATC 1 cut(s) 451
Esp3I CGTCTC 3 cut(s) 76, 109, 142
FaeI CATG 5 cut(s) 463, 524, 719, 749, 759
FaqI GGGAC 2 cut(s) 218, 618
FatI CATG 5 cut(s) 459, 520, 715, 745, 755
FauNDI CATATG 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 252, 279, 713, 743
Fsp4HI GCNGC 4 cut(s) 252, 279, 713, 743
FspBI CTAG 1 cut(s) 225
GluI GCNGC 4 cut(s) 252, 279, 713, 743
HaeIII GGCC 2 cut(s) 61, 416
HapII CCGG 3 cut(s) 123, 168, 553
HgaI GACGC 2 cut(s) 82, 91
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 5 cut(s) 463, 524, 719, 749, 759
HinfI GANTC 2 cut(s) 164, 706
HpaII CCGG 3 cut(s) 123, 168, 553
HphI GGTGA 3 cut(s) 109, 418, 446
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 3 cut(s) 109, 205, 289
Hpy188III TCNNGA 5 cut(s) 23, 46, 168, 521, 756
Hpy8I GTNNAC 1 cut(s) 242
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 387
HpyCH4III ACNGT 2 cut(s) 43, 70
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 5 cut(s) 281, 639, 660, 715, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 1 cut(s) 575
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 5 cut(s) 463, 524, 719, 749, 759
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Lsp1109I GCAGC 4 cut(s) 238, 265, 699, 729
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 2 cut(s) 9, 115
MboII GAAGA 5 cut(s) 275, 320, 449, 455, 485
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 2 cut(s) 640, 689
MreI CGCCGGCG 1 cut(s) 122
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 712
MspI CCGG 3 cut(s) 123, 168, 553
MspR9I CCNGG 3 cut(s) 154, 501, 553
MvaI CCWGG 2 cut(s) 154, 501
MwoI GCNNNNNNNGC 1 cut(s) 99
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 553
NdeI CATATG 1 cut(s) 559
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 5 cut(s) 463, 524, 719, 749, 759
NlaIV GGNNCC 3 cut(s) 40, 417, 551
NmuCI GTSAC 2 cut(s) 9, 115
NspI RCATGY 2 cut(s) 719, 749
PagI TCATGA 2 cut(s) 520, 755
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 164, 706
PkrI GCNGC 4 cut(s) 253, 280, 714, 744
Ppu21I YACGTR 1 cut(s) 576
Psp6I CCWGG 2 cut(s) 152, 499
PspGI CCWGG 2 cut(s) 152, 499
PspN4I GGNNCC 3 cut(s) 40, 417, 551
PspPI GGNCC 3 cut(s) 59, 415, 549
PstI CTGCAG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 712
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SatI GCNGC 4 cut(s) 252, 279, 713, 743
Sau96I GGNCC 3 cut(s) 59, 415, 549
ScrFI CCNGG 3 cut(s) 154, 501, 553
SduI GDGCHC 1 cut(s) 149
SexAI ACCWGGT 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 279
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 549
SnaBI TACGTA 1 cut(s) 576
Sse9I AATT 2 cut(s) 640, 689
SsiI CCGC 2 cut(s) 75, 120
SspI AATATT 2 cut(s) 541, 587
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 152, 499, 551
TaaI ACNGT 2 cut(s) 43, 70
TaiI ACGT 1 cut(s) 578
TaqI TCGA 2 cut(s) 45, 300
TasI AATT 2 cut(s) 640, 689
TfiI GAWTC 2 cut(s) 164, 706
TseFI GTSAC 2 cut(s) 9, 115
TseI GCWGC 4 cut(s) 251, 278, 712, 742
Tsp45I GTSAC 2 cut(s) 9, 115
TspDTI ATGAA 4 cut(s) 321, 456, 486, 537
TspGWI ACGGA 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 549
XceI RCATGY 2 cut(s) 719, 749
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.