RchiOBHm_Chr1g0346321

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005075
Physical Location & Seq
Reverse (-)
38445824 .. 38451050
5227 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57259

Sequence Viewer

Length: 1110 bp
ATGTCCGGACCCTCAAGATATACTCGCAAACTTGGTGATACGTCGTCGCATCGCCTTGAAAACGGCGTTCGGCCTATAAACGACACCACCACAACCATCGTATATTCCGACTCTCTCTCTCTCTCTCTCTCCTTGTCGAAGCGAAGCAAAGCAAAAGCAGCGGAGGTTGAAGACGACGAGGACGACATGGTTCTGTCACAGAGGCTTCACGAGGCGTTCAAAGGCACCGTCGAGAAAATCACAGGGCCTCGCACCGTCTCCGCTTTCAAAGAGAAAGGCGTCCTCAGCGTCTCCGAGTTCGTCACCGCCGGCGATAACCTCGTCTCCAAGTGCCCTACCTGGTCTTGGGAATCCGGAGAGCCAAGCAAGAGGAAGCCATACCTACCATCTGAAAAGCAGTATTTGATTACTAGAAATGTCCCTTGTTTACGCAGGGCTGCTTGTGTGGAAGAGGAGTACGAAGCTGCAGGTTCGGAAGTTCTTATCGATAATGAAGATAATGATGGCTGGTTGGCAACACACGGGAAGCCAAAGGATAAAAGTAGTGAGGAGGAAAACTTTCCTTCTATTGAAAGTTTAGAAATCAGCAAGGTGAGGTCGGCCCCCTCGCAAGTTGGGGGTGAAGGTGAAGATGAAGATGATATCCCTGACATGGCTGATTATGAAGAAGCTGACAACATTGAAATAGATCCTGGAACATATCTTGTTGCTCATGAACCTGATGATGACAATATTTTACGGACCCGGACATATGATGTCAGCATTACGTATGATAAATATTACCAAACTCCTCGGGTGTGGCTTACTGGCTATGATGAGTCAAGAATGCTTTTACAAGCAGAGCTTGTACTTGAAGATGTCAGTCAAGATCATGCACGCAAAACTGTAACTATTGAGGATCATCCGCACTTGCCCGGAAAGCATGCATCTGTCCATCCATGCCGACATGGGGCTGTGATGAAGAAAATAATTGATGTACTAATGTCACGTGGAGTGGAACCGGAAGTCGATAAGTACCTCTTCTTATTCTTGAAATTTGTTGCCTCGGTGATTCCAACCATCGAATATGACTATACGATGGACTTTGATCTTGGCAGCTCCAGCAGTTGA

Protein Analysis

369

Amino Acids

41.37

Weight (kDa)

4.75

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 99 - 358 3.3e-59 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 224
AccIII TCCGGA 2 cut(s) 5, 353
AciI CCGC 4 cut(s) 161, 261, 306, 905
AclWI GGATC 2 cut(s) 683, 906
AcsI RAATTY 1 cut(s) 1034
AcvI CACGTG 1 cut(s) 989
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 458, 849, 978, 1016
AfiI CCNNNNNNNGG 3 cut(s) 345, 652, 949
AgsI TTSAA 8 cut(s) 59, 170, 220, 268, 572, 683, 854, 1033
AjnI CCWGG 2 cut(s) 338, 691
AluBI AGCT 4 cut(s) 464, 671, 844, 1098
AluI AGCT 4 cut(s) 464, 671, 844, 1098
Alw26I GTCTC 3 cut(s) 262, 295, 328
AlwI GGATC 2 cut(s) 683, 906
Ama87I CYCGRG 1 cut(s) 792
Aor13HI TCCGGA 2 cut(s) 5, 353
AoxI GGCC 3 cut(s) 71, 245, 600
ApeKI GCWGC 4 cut(s) 158, 437, 464, 1095
ApoI RAATTY 1 cut(s) 1034
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
Asp700I GAANNNNTTC 1 cut(s) 558
AspS9I GGNCC 4 cut(s) 8, 245, 601, 741
AsuC2I CCSGG 2 cut(s) 745, 915
AsuHPI GGTGA 6 cut(s) 47, 295, 604, 632, 638, 1060
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 2 cut(s) 8, 741
BaeGI GKGCMC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 224
BauI CACGAG 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 170, 424, 451
BccI CCATC 6 cut(s) 104, 394, 497, 942, 1067, 1072
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 2 cut(s) 340, 693
BcnI CCSGG 2 cut(s) 745, 915
BcoDI GTCTC 3 cut(s) 262, 295, 328
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 4 cut(s) 159, 438, 465, 1096
BlsI GCNGC 4 cut(s) 160, 439, 466, 1097
Bme1390I CCNGG 4 cut(s) 340, 693, 745, 915
Bme18I GGWCC 2 cut(s) 8, 741
BmeT110I CYCGRG 1 cut(s) 792
BmgT120I GGNCC 4 cut(s) 8, 245, 601, 741
BmiI GGNNCC 5 cut(s) 10, 226, 603, 743, 999
BmrFI CCNGG 4 cut(s) 340, 693, 745, 915
BmsI GCATC 2 cut(s) 58, 935
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 1084
Bpu10I CCTNAGC 1 cut(s) 284
BpuMI CCSGG 2 cut(s) 745, 915
Bsa29I ATCGAT 1 cut(s) 486
BsaAI YACGTR 2 cut(s) 768, 989
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 791, 1044
BsaWI WCCGGW 3 cut(s) 5, 353, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 652, 949
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 1 cut(s) 811
BseAI TCCGGA 2 cut(s) 5, 353
BseBI CCWGG 2 cut(s) 340, 693
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 791, 1044
BseGI GGATG 2 cut(s) 901, 934
BseLI CCNNNNNNNGG 3 cut(s) 345, 652, 949
BseMII CTCAG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 811
BseRI GAGGAG 3 cut(s) 467, 563, 780
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 3 cut(s) 170, 424, 451
BshFI GGCC 3 cut(s) 73, 247, 602
BshNI GGYRCC 1 cut(s) 224
BshVI ATCGAT 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 6 cut(s) 6, 309, 354, 745, 915, 1001
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 3 cut(s) 345, 652, 949
BsmAI GTCTC 3 cut(s) 262, 295, 328
BsmBI CGTCTC 3 cut(s) 262, 295, 328
BsmFI GGGAC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 3 cut(s) 73, 247, 602
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 335
Bsp13I TCCGGA 2 cut(s) 5, 353
Bsp143I GATC 4 cut(s) 688, 868, 898, 1087
BspACI CCGC 4 cut(s) 161, 261, 306, 905
BspANI GGCC 3 cut(s) 73, 247, 602
BspCNI CTCAG 1 cut(s) 297
BspDI ATCGAT 1 cut(s) 486
BspEI TCCGGA 2 cut(s) 5, 353
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 5 cut(s) 10, 226, 603, 743, 999
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 2 cut(s) 683, 906
BspT107I GGYRCC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 1 cut(s) 811
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 791, 1044
BssMI GATC 4 cut(s) 688, 868, 898, 1087
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 340, 693
Bst4CI ACNGT 3 cut(s) 229, 256, 886
Bst6I CTCTTC 2 cut(s) 444, 1025
BstACI GRCGYC 1 cut(s) 279
BstBAI YACGTR 2 cut(s) 768, 989
BstC8I GCNNGC 3 cut(s) 310, 877, 924
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 901, 934
BstKTI GATC 4 cut(s) 691, 871, 901, 1090
BstMAI GTCTC 3 cut(s) 262, 295, 328
BstMBI GATC 4 cut(s) 688, 868, 898, 1087
BstMWI GCNNNNNNNGC 4 cut(s) 158, 285, 919, 1101
BstNI CCWGG 2 cut(s) 340, 693
BstNSI RCATGY 1 cut(s) 926
BstSCI CCNGG 4 cut(s) 338, 691, 743, 913
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 335
BstSNI TACGTA 1 cut(s) 768
BstV1I GCAGC 3 cut(s) 170, 424, 451
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 486
BsuRI GGCC 3 cut(s) 73, 247, 602
BsuTUI ATCGAT 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 35
BtsCI GGATG 2 cut(s) 901, 934
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 3 cut(s) 310, 877, 924
CciI TCATGA 1 cut(s) 712
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 4 cut(s) 8, 245, 601, 741
ClaI ATCGAT 1 cut(s) 486
CseI GACGC 2 cut(s) 268, 277
CsiI ACCWGGT 1 cut(s) 338
Csp6I GTAC 4 cut(s) 457, 848, 977, 1015
CviAII CATG 7 cut(s) 187, 652, 713, 872, 923, 939, 947
CviQI GTAC 4 cut(s) 457, 848, 977, 1015
DdeI CTNAG 1 cut(s) 284
DpnI GATC 4 cut(s) 690, 870, 900, 1089
DpnII GATC 4 cut(s) 688, 868, 898, 1087
Eam1104I CTCTTC 2 cut(s) 444, 1025
EarI CTCTTC 2 cut(s) 444, 1025
Eco105I TACGTA 1 cut(s) 768
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 8, 741
Eco72I CACGTG 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 338, 691
EcoRV GATATC 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 928
Esp3I CGTCTC 3 cut(s) 262, 295, 328
FaeI CATG 7 cut(s) 190, 655, 716, 875, 926, 942, 950
FalI AAGNNNNNCTT 4 cut(s) 828, 860, 1004, 1036
FaqI GGGAC 1 cut(s) 404
FatI CATG 7 cut(s) 186, 651, 712, 871, 922, 938, 946
FauNDI CATATG 1 cut(s) 751
Fnu4HI GCNGC 4 cut(s) 159, 438, 465, 1096
FokI GGATG 2 cut(s) 888, 921
Fsp4HI GCNGC 4 cut(s) 159, 438, 465, 1096
FspBI CTAG 1 cut(s) 411
GluI GCNGC 4 cut(s) 159, 438, 465, 1096
GsuI CTGGAG 1 cut(s) 1084
HaeIII GGCC 3 cut(s) 73, 247, 602
HapII CCGG 6 cut(s) 6, 309, 354, 745, 915, 1001
HgaI GACGC 2 cut(s) 268, 277
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 7 cut(s) 190, 655, 716, 875, 926, 942, 950
HinfI GANTC 4 cut(s) 110, 350, 818, 1051
HpaII CCGG 6 cut(s) 6, 309, 354, 745, 915, 1001
HphI GGTGA 6 cut(s) 47, 295, 604, 632, 638, 1060
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 4 cut(s) 109, 295, 391, 475
Hpy188III TCNNGA 9 cut(s) 6, 15, 209, 232, 354, 713, 822, 866, 1030
Hpy8I GTNNAC 1 cut(s) 428
Hpy99I CGWCG 4 cut(s) 46, 49, 179, 233
HpyAV CCTTC 2 cut(s) 573, 617
HpyCH4III ACNGT 3 cut(s) 229, 256, 886
HpyCH4IV ACGT 3 cut(s) 41, 767, 988
HpyCH4V TGCA 3 cut(s) 467, 875, 926
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 285, 919, 1101
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 3 cut(s) 41, 767, 988
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 7 cut(s) 190, 655, 716, 875, 926, 942, 950
Kpn2I TCCGGA 2 cut(s) 5, 353
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Kzo9I GATC 4 cut(s) 688, 868, 898, 1087
LmnI GCTCC 1 cut(s) 1103
Lsp1109I GCAGC 3 cut(s) 170, 424, 451
LweI GCATC 2 cut(s) 58, 935
MabI ACCWGGT 1 cut(s) 338
MaeI CTAG 1 cut(s) 411
MaeII ACGT 3 cut(s) 41, 767, 988
MaeIII GTNAC 4 cut(s) 195, 301, 886, 984
MalI GATC 4 cut(s) 690, 870, 900, 1089
MboI GATC 4 cut(s) 688, 868, 898, 1087
MboII GAAGA 9 cut(s) 182, 461, 506, 641, 647, 677, 866, 973, 1012
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 2 cut(s) 969, 1034
MlyI GAGTC 2 cut(s) 104, 827
MmeI TCCRAC 2 cut(s) 132, 1079
Mph1103I ATGCAT 1 cut(s) 928
MreI CGCCGGCG 1 cut(s) 308
MroI TCCGGA 2 cut(s) 5, 353
MroNI GCCGGC 1 cut(s) 308
MroXI GAANNNNTTC 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 6 cut(s) 6, 309, 354, 745, 915, 1001
MspR9I CCNGG 4 cut(s) 340, 693, 745, 915
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 2 cut(s) 340, 693
MwoI GCNNNNNNNGC 4 cut(s) 158, 285, 919, 1101
NaeI GCCGGC 1 cut(s) 310
NciI CCSGG 2 cut(s) 745, 915
NdeI CATATG 1 cut(s) 751
NdeII GATC 4 cut(s) 688, 868, 898, 1087
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 7 cut(s) 190, 655, 716, 875, 926, 942, 950
NlaIV GGNNCC 5 cut(s) 10, 226, 603, 743, 999
NmuCI GTSAC 3 cut(s) 195, 301, 984
NsiI ATGCAT 1 cut(s) 928
NspI RCATGY 1 cut(s) 926
PaeI GCATGC 1 cut(s) 926
PagI TCATGA 1 cut(s) 712
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 180
PctI GAATGC 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 350, 1051
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 4 cut(s) 160, 439, 466, 1097
PleI GAGTC 2 cut(s) 104, 826
PmaCI CACGTG 1 cut(s) 989
PmlI CACGTG 1 cut(s) 989
PpsI GAGTC 2 cut(s) 104, 826
Ppu21I YACGTR 2 cut(s) 768, 989
Psp6I CCWGG 2 cut(s) 338, 691
PspCI CACGTG 1 cut(s) 989
PspGI CCWGG 2 cut(s) 338, 691
PspN4I GGNNCC 5 cut(s) 10, 226, 603, 743, 999
PspPI GGNCC 4 cut(s) 8, 245, 601, 741
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 4 cut(s) 458, 849, 978, 1016
RsaNI GTAC 4 cut(s) 457, 848, 977, 1015
SatI GCNGC 4 cut(s) 159, 438, 465, 1096
Sau3AI GATC 4 cut(s) 688, 868, 898, 1087
Sau96I GGNCC 4 cut(s) 8, 245, 601, 741
SchI GAGTC 2 cut(s) 104, 827
ScrFI CCNGG 4 cut(s) 340, 693, 745, 915
SduI GDGCHC 1 cut(s) 335
SexAI ACCWGGT 1 cut(s) 338
SfaNI GCATC 2 cut(s) 58, 935
SfcI CTRYAG 1 cut(s) 465
SgrAI CRCCGGYG 1 cut(s) 308
SinI GGWCC 2 cut(s) 8, 741
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
SnaBI TACGTA 1 cut(s) 768
SphI GCATGC 1 cut(s) 926
Sse9I AATT 2 cut(s) 969, 1034
SsiI CCGC 4 cut(s) 161, 261, 306, 905
SspI AATATT 2 cut(s) 733, 779
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 4 cut(s) 338, 691, 743, 913
TaaI ACNGT 3 cut(s) 229, 256, 886
TaiI ACGT 3 cut(s) 44, 770, 991
TaqI TCGA 5 cut(s) 137, 231, 486, 1008, 1062
TasI AATT 2 cut(s) 969, 1034
TatI WGTACW 2 cut(s) 847, 976
TfiI GAWTC 2 cut(s) 350, 1051
TseFI GTSAC 3 cut(s) 195, 301, 984
TseI GCWGC 4 cut(s) 158, 437, 464, 1095
Tsp45I GTSAC 3 cut(s) 195, 301, 984
TspDTI ATGAA 5 cut(s) 507, 648, 678, 729, 974
TspGWI ACGGA 1 cut(s) 754
VpaK11BI GGWCC 2 cut(s) 8, 741
XapI RAATTY 1 cut(s) 1034
XceI RCATGY 1 cut(s) 926
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.