Rh1DG195300

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005075
Physical Location & Seq
Reverse (-)
38584879 .. 38591573
6695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG195300.1

Sequence Viewer

Length: 870 bp
ATGGTTCTGTCACAGAGGCTTCACGAGGCGTTCAAAGGCACCGTCGAGAAAATCACAGGGCCTCGCACCGTCTCCGCTTTCAAAGAGAAAGGCGTCCTCAGCGTCTCCGAGTTCGTCACCGCCGGCGATAACCTCGTCTCCAAGTGCCCTACCTGGTCTTGGGAATCCGGAGAGCCAAGCAAGAGGAAGCCATACCTACCATCTGAAAAGCAGTATTTGATTACTAGAAATGTCCCTTGTTTACGCAGGGCTGCTTGTGTGGAAGAGGAGTATGAAGCTGCAGGTTCGGAAGTTCTTATCGATAACGAAGATAATGATGGCTGGTTGGCAACACACGGGAAGCCAAAGGATAAAAGTAGTGAGGAGGAAAACTTTCCTTCCATTGAAAGTTTAGAAATCAGCAAGGTGAAGTCGGCCCCCTCGCAAGTTGGGGGTGAAGGTGAAGATGAAGATGATATCCCTGACATGGCTGATTATGAAGAAGCTGACAACATTGAAATAGATCCTGGAACATATCTTGTTGCTCATGAACCTGATGATGACAATATTTTACGGACCCGGACATATGATGTCAGCATTACGTATGATAAATATTACCAAACTCCTCGGGTGTGGCTTACTGGCTATGATGAGGTTTGTCCCTGCAATTTTATCTTTTCCTTTTGCAGGTGTATTATTATCAATAAATGCTGTCAATTTGGTATTCAACTGAATCAGCTGCATGTGTTTTTTTTTTTTGGTCAAAGAGCTGCATGTTTGGTCATGACCAAAGAATGTTTCATGACAAGAGATTTTATTGCTCCCAATGTTCTGTGGCTTATTGTGCTCAACATTGTCAGGGTTGATTTCTGTGATTTCACACAGGATTAA

Protein Analysis

289

Amino Acids

32.89

Weight (kDa)

4.61

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 37 - 211 1.1e-26 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 657
Acc36I ACCTGC 2 cut(s) 272, 657
AccB1I GGYRCC 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 2 cut(s) 75, 120
AclWI GGATC 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 93
AfiI CCNNNNNNNGG 3 cut(s) 159, 466, 666
AgsI TTSAA 5 cut(s) 34, 82, 386, 497, 707
AjnI CCWGG 2 cut(s) 152, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 761, 793
AluBI AGCT 4 cut(s) 278, 485, 718, 749
AluI AGCT 4 cut(s) 278, 485, 718, 749
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 3 cut(s) 76, 109, 142
AlwI GGATC 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 2 cut(s) 59, 414
ApeKI GCWGC 4 cut(s) 251, 278, 718, 749
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 59, 415, 555
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 4 cut(s) 109, 418, 446, 452
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 149
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 828
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 238, 265, 705, 736
BccI CCATC 2 cut(s) 208, 311
BciT130I CCWGG 2 cut(s) 154, 507
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 3 cut(s) 76, 109, 142
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 2 cut(s) 272, 657
BisI GCNGC 4 cut(s) 252, 279, 719, 750
BlsI GCNGC 4 cut(s) 253, 280, 720, 751
Bme1390I CCNGG 3 cut(s) 154, 507, 559
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 3 cut(s) 59, 415, 555
BmiI GGNNCC 3 cut(s) 40, 417, 557
BmrFI CCNGG 3 cut(s) 154, 507, 559
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 605
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 466, 666
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 625
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 2 cut(s) 154, 507
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 605
BseLI CCNNNNNNNGG 3 cut(s) 159, 466, 666
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 3 cut(s) 281, 377, 594
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 4 cut(s) 238, 265, 705, 736
BshFI GGCC 2 cut(s) 61, 416
BshNI GGYRCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 3 cut(s) 123, 168, 559
BslFI GGGAC 2 cut(s) 218, 624
BslI CCNNNNNNNGG 3 cut(s) 159, 466, 666
BsmAI GTCTC 3 cut(s) 76, 109, 142
BsmBI CGTCTC 3 cut(s) 76, 109, 142
BsmFI GGGAC 2 cut(s) 218, 624
BsnI GGCC 2 cut(s) 61, 416
BsoBI CYCGRG 1 cut(s) 606
Bsp1286I GDGCHC 2 cut(s) 149, 828
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 1 cut(s) 502
BspACI CCGC 2 cut(s) 75, 120
BspANI GGCC 2 cut(s) 61, 416
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 300
BspEI TCCGGA 1 cut(s) 167
BspHI TCATGA 3 cut(s) 526, 762, 780
BspLI GGNNCC 3 cut(s) 40, 417, 557
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 2 cut(s) 272, 657
BspPI GGATC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 625
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 605
BssMI GATC 1 cut(s) 502
BssNI GRCGYC 1 cut(s) 93
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 154, 507
Bst4CI ACNGT 2 cut(s) 43, 70
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 98
BstENI CCTNNNNNAGG 1 cut(s) 664
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 3 cut(s) 76, 109, 142
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 2 cut(s) 99, 823
BstNI CCWGG 2 cut(s) 154, 507
BstNSI RCATGY 2 cut(s) 725, 756
BstSCI CCNGG 3 cut(s) 152, 505, 557
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 582
BstV1I GCAGC 4 cut(s) 238, 265, 705, 736
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 2 cut(s) 61, 416
BsuTUI ATCGAT 1 cut(s) 300
BveI ACCTGC 2 cut(s) 272, 657
Cac8I GCNNGC 1 cut(s) 124
CciI TCATGA 3 cut(s) 526, 762, 780
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 59, 415, 555
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 2 cut(s) 82, 91
CsiI ACCWGGT 1 cut(s) 152
CviAII CATG 6 cut(s) 466, 527, 722, 753, 763, 781
DdeI CTNAG 1 cut(s) 98
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 582
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 606
EcoNI CCTNNNNNAGG 1 cut(s) 664
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 152, 505
EcoRV GATATC 1 cut(s) 457
Esp3I CGTCTC 3 cut(s) 76, 109, 142
FaeI CATG 6 cut(s) 469, 530, 725, 756, 766, 784
FaqI GGGAC 2 cut(s) 218, 624
FatI CATG 6 cut(s) 465, 526, 721, 752, 762, 780
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 4 cut(s) 252, 279, 719, 750
Fsp4HI GCNGC 4 cut(s) 252, 279, 719, 750
FspBI CTAG 1 cut(s) 225
GluI GCNGC 4 cut(s) 252, 279, 719, 750
HaeIII GGCC 2 cut(s) 61, 416
HapII CCGG 3 cut(s) 123, 168, 559
HgaI GACGC 2 cut(s) 82, 91
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 6 cut(s) 469, 530, 725, 756, 766, 784
HinfI GANTC 2 cut(s) 164, 712
HpaII CCGG 3 cut(s) 123, 168, 559
HphI GGTGA 4 cut(s) 109, 418, 446, 452
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 3 cut(s) 109, 205, 289
Hpy188III TCNNGA 6 cut(s) 23, 46, 168, 527, 763, 781
Hpy8I GTNNAC 1 cut(s) 242
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 387, 431
HpyCH4III ACNGT 2 cut(s) 43, 70
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 5 cut(s) 281, 645, 666, 721, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 823
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 1 cut(s) 581
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 6 cut(s) 469, 530, 725, 756, 766, 784
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 4 cut(s) 238, 265, 705, 736
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 2 cut(s) 9, 115
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 5 cut(s) 275, 320, 455, 461, 491
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 2 cut(s) 149, 828
MluCI AATT 2 cut(s) 646, 695
MreI CGCCGGCG 1 cut(s) 122
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 1 cut(s) 868
MspA1I CMGCKG 1 cut(s) 718
MspI CCGG 3 cut(s) 123, 168, 559
MspR9I CCNGG 3 cut(s) 154, 507, 559
MvaI CCWGG 2 cut(s) 154, 507
MwoI GCNNNNNNNGC 2 cut(s) 99, 823
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 559
NdeI CATATG 1 cut(s) 565
NdeII GATC 1 cut(s) 502
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 6 cut(s) 469, 530, 725, 756, 766, 784
NlaIV GGNNCC 3 cut(s) 40, 417, 557
NmuCI GTSAC 2 cut(s) 9, 115
NspI RCATGY 2 cut(s) 725, 756
PagI TCATGA 3 cut(s) 526, 762, 780
PaqCI CACCTGC 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 164, 712
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 4 cut(s) 253, 280, 720, 751
Ppu21I YACGTR 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 152, 505
PspGI CCWGG 2 cut(s) 152, 505
PspN4I GGNNCC 3 cut(s) 40, 417, 557
PspPI GGNCC 3 cut(s) 59, 415, 555
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 718
SaqAI TTAA 1 cut(s) 868
SatI GCNGC 4 cut(s) 252, 279, 719, 750
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 3 cut(s) 59, 415, 555
ScrFI CCNGG 3 cut(s) 154, 507, 559
SduI GDGCHC 2 cut(s) 149, 828
SexAI ACCWGGT 1 cut(s) 152
SfcI CTRYAG 1 cut(s) 279
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 555
SnaBI TACGTA 1 cut(s) 582
Sse9I AATT 2 cut(s) 646, 695
SsiI CCGC 2 cut(s) 75, 120
SspI AATATT 2 cut(s) 547, 593
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 152, 505, 557
TaaI ACNGT 2 cut(s) 43, 70
TaiI ACGT 1 cut(s) 584
TaqI TCGA 2 cut(s) 45, 300
TasI AATT 2 cut(s) 646, 695
TfiI GAWTC 2 cut(s) 164, 712
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TseFI GTSAC 2 cut(s) 9, 115
TseI GCWGC 4 cut(s) 251, 278, 718, 749
Tsp45I GTSAC 2 cut(s) 9, 115
TspDTI ATGAA 5 cut(s) 288, 462, 492, 543, 769
TspGWI ACGGA 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 555
XagI CCTNNNNNAGG 1 cut(s) 664
XceI RCATGY 2 cut(s) 725, 756
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.