MD02G1218100.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005075
Physical Location & Seq
Reverse (-)
25346279 .. 25351673
5395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1218100.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGTTCTGTCACAGAAGCTGCACGAGGCGTTCAAAGGAACGGTGGAGAAAATCACAGGTCCCCGCACCGTCTCCGCCTTCAAAGAAAAAGGCGTTCTCAGCGTCTCCGAGTTCGTCACAGCTGGCGATAACCTCGTCTCTAAGTGCCCCACCTGGTCCTGGGAATCTGGCGAGCCGAGCAAGAGGAAGGCGTACTTACCATCGGAGAAACAGTATTTGATTACTAGAAATGTGCCGTGCTTACGAAGGGCTGCATATTTGGAAGAGGAGTATGAAGCTGCTGGTATGGAAGTTCTTCTTGATAATGAAGATAATGATGGCTGGTTGGCAACCCATGGGAAGCCAAAGGATACAAGTGGTGAGGTGGAAAACTTACCCTCCATTGAAAGTTTAGAAATCAGCAAGAATAGGGATGTGAAATCGGTCCCCTCGCATGTTGGAGTGGAAGATGAAGATGATATTCCGGACATGGCTGACTTTGAAGAAGCTGACAACATTGAGATTGATCCTGGAACATATCTTATTGCTCATGAACCTGACGATGACAATATTTTACGAACCCGAACATATGATGTCAGCATCACATATGATAAATATTACCAGACTCCTCGAGTGTGGCTTACTGGGTATGATGAGTCAAGGATGCTTTTACAAGCAGAGCTCGTACTTGAAGATGTCAGTCAAGATCATGCACGCAAAACTGTAACTATTGAGGATCATCCCCACTTGTCTGGAAAGCATGCATCTGTACATCCATGCCGACATGGGGCTGTGATGAAAAAAATTATTGATGTGCTAATGTCACGTGGAGTGGAACCAGAAGTTGATAAGTACCTATTCTTATTCTTGAAATTTGTTGCCTCTGTAATTCCAACTATCGAATATGATTATACGATGGACTTTGATCTCGGCAGCTCCAGCAGTTAG

Protein Analysis

309

Amino Acids

34.76

Weight (kDa)

4.75

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 37 - 297 9.5e-60 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 2 cut(s) 64, 75
AclWI GGATC 2 cut(s) 500, 723
AcsI RAATTY 1 cut(s) 851
AcvI CACGTG 1 cut(s) 806
AfaI GTAC 4 cut(s) 194, 666, 750, 833
AfiI CCNNNNNNNGG 2 cut(s) 159, 766
AgsI TTSAA 6 cut(s) 34, 82, 386, 482, 671, 850
AjnI CCWGG 3 cut(s) 152, 158, 508
AluBI AGCT 6 cut(s) 19, 122, 278, 488, 661, 915
AluI AGCT 6 cut(s) 19, 122, 278, 488, 661, 915
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 3 cut(s) 76, 109, 142
AlwI GGATC 2 cut(s) 500, 723
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 609
Aor13HI TCCGGA 1 cut(s) 463
ApeKI GCWGC 4 cut(s) 19, 251, 278, 912
ApoI RAATTY 1 cut(s) 851
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 3 cut(s) 59, 156, 424
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 609
AvaII GGWCC 3 cut(s) 59, 156, 424
BaeGI GKGCMC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 663
BauI CACGAG 1 cut(s) 23
BbrPI CACGTG 1 cut(s) 806
Bbv12I GWGCWC 1 cut(s) 663
BbvI GCAGC 3 cut(s) 6, 238, 265
BccI CCATC 3 cut(s) 208, 311, 889
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 3 cut(s) 154, 160, 510
BciVI GTATCC 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 76, 109, 142
BfaI CTAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 4 cut(s) 20, 252, 279, 913
BlsI GCNGC 4 cut(s) 21, 253, 280, 914
Bme1390I CCNGG 3 cut(s) 154, 160, 510
Bme18I GGWCC 3 cut(s) 59, 156, 424
BmeT110I CYCGRG 1 cut(s) 609
BmgT120I GGNCC 3 cut(s) 59, 156, 424
BmiI GGNNCC 3 cut(s) 61, 426, 816
BmrFI CCNGG 3 cut(s) 154, 160, 510
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 3 cut(s) 588, 633, 752
BmuI ACTGGG 1 cut(s) 633
BpmI CTGGAG 1 cut(s) 901
BsaAI YACGTR 1 cut(s) 806
BsaJI CCNNGG 2 cut(s) 159, 334
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 766
Bse1I ACTGG 1 cut(s) 628
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 3 cut(s) 154, 160, 510
BseDI CCNNGG 2 cut(s) 159, 334
BseGI GGATG 4 cut(s) 418, 648, 718, 751
BseLI CCNNNNNNNGG 2 cut(s) 159, 766
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 628
BseRI GAGGAG 2 cut(s) 281, 597
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 3 cut(s) 6, 238, 265
BsgI GTGCAG 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 663
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 2 cut(s) 45, 410
BslI CCNNNNNNNGG 2 cut(s) 159, 766
BsmAI GTCTC 3 cut(s) 76, 109, 142
BsmBI CGTCTC 3 cut(s) 76, 109, 142
BsmFI GGGAC 2 cut(s) 45, 410
BsoBI CYCGRG 1 cut(s) 609
Bsp1286I GDGCHC 2 cut(s) 149, 663
Bsp13I TCCGGA 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 748
Bsp143I GATC 4 cut(s) 505, 685, 715, 904
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 2 cut(s) 64, 75
BspCNI CTCAG 1 cut(s) 111
BspEI TCCGGA 1 cut(s) 463
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 61, 426, 816
BspPI GGATC 2 cut(s) 500, 723
BsrGI TGTACA 1 cut(s) 748
BsrI ACTGG 1 cut(s) 628
BssECI CCNNGG 2 cut(s) 159, 334
BssMI GATC 4 cut(s) 505, 685, 715, 904
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 154, 160, 510
Bst4CI ACNGT 4 cut(s) 43, 70, 213, 703
Bst6I CTCTTC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 748
BstBAI YACGTR 1 cut(s) 806
BstC8I GCNNGC 4 cut(s) 124, 173, 694, 741
BstDEI CTNAG 2 cut(s) 98, 141
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 418, 648, 718, 751
BstKTI GATC 4 cut(s) 508, 688, 718, 907
BstMAI GTCTC 3 cut(s) 76, 109, 142
BstMBI GATC 4 cut(s) 505, 685, 715, 904
BstMWI GCNNNNNNNGC 3 cut(s) 99, 177, 918
BstNI CCWGG 3 cut(s) 154, 160, 510
BstNSI RCATGY 2 cut(s) 437, 743
BstSCI CCNGG 3 cut(s) 152, 158, 508
BstSLI GKGCMC 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 6, 238, 265
BstXI CCANNNNNNTGG 1 cut(s) 731
BsuI GTATCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 4 cut(s) 418, 648, 718, 751
Cac8I GCNNGC 4 cut(s) 124, 173, 694, 741
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 529
Cfr13I GGNCC 3 cut(s) 59, 156, 424
CseI GACGC 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 4 cut(s) 193, 665, 749, 832
CviAII CATG 8 cut(s) 335, 434, 469, 530, 689, 740, 756, 764
CviQI GTAC 4 cut(s) 193, 665, 749, 832
DdeI CTNAG 2 cut(s) 98, 141
DpnI GATC 4 cut(s) 507, 687, 717, 906
DpnII GATC 4 cut(s) 505, 685, 715, 904
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 663
Eco47I GGWCC 3 cut(s) 59, 156, 424
Eco53kI GAGCTC 1 cut(s) 661
Eco72I CACGTG 1 cut(s) 806
Eco88I CYCGRG 1 cut(s) 609
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 3 cut(s) 152, 158, 508
EcoT14I CCWWGG 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 334
Esp3I CGTCTC 3 cut(s) 76, 109, 142
FaeI CATG 8 cut(s) 338, 437, 472, 533, 692, 743, 759, 767
FalI AAGNNNNNCTT 4 cut(s) 179, 211, 282, 314
FaqI GGGAC 2 cut(s) 45, 410
FatI CATG 8 cut(s) 334, 433, 468, 529, 688, 739, 755, 763
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 2 cut(s) 568, 586
Fnu4HI GCNGC 4 cut(s) 20, 252, 279, 913
FokI GGATG 4 cut(s) 425, 655, 705, 738
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 4 cut(s) 20, 252, 279, 913
FspBI CTAG 1 cut(s) 225
GluI GCNGC 4 cut(s) 20, 252, 279, 913
GsuI CTGGAG 1 cut(s) 901
HapII CCGG 1 cut(s) 464
HgaI GACGC 1 cut(s) 91
Hin1II CATG 8 cut(s) 338, 437, 472, 533, 692, 743, 759, 767
HinfI GANTC 3 cut(s) 164, 604, 635
HpaII CCGG 1 cut(s) 464
HphI GGTGA 1 cut(s) 371
Hpy188I TCNGA 2 cut(s) 109, 205
Hpy188III TCNNGA 6 cut(s) 299, 464, 530, 683, 732, 847
HpyAV CCTTC 3 cut(s) 88, 181, 240
HpyCH4III ACNGT 4 cut(s) 43, 70, 213, 703
HpyCH4IV ACGT 1 cut(s) 805
HpyCH4V TGCA 4 cut(s) 22, 254, 692, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 177, 918
HpyF3I CTNAG 2 cut(s) 98, 141
HpySE526I ACGT 1 cut(s) 805
Hsp92II CATG 8 cut(s) 338, 437, 472, 533, 692, 743, 759, 767
Kpn2I TCCGGA 1 cut(s) 463
Kzo9I GATC 4 cut(s) 505, 685, 715, 904
LmnI GCTCC 1 cut(s) 920
Lsp1109I GCAGC 3 cut(s) 6, 238, 265
LweI GCATC 3 cut(s) 588, 633, 752
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 805
MaeIII GTNAC 4 cut(s) 9, 115, 703, 801
MalI GATC 4 cut(s) 507, 687, 717, 906
MboI GATC 4 cut(s) 505, 685, 715, 904
MboII GAAGA 7 cut(s) 275, 287, 320, 458, 464, 494, 683
MhlI GDGCHC 2 cut(s) 149, 663
MluCI AATT 3 cut(s) 783, 851, 867
MlyI GAGTC 2 cut(s) 598, 644
MmeI TCCRAC 2 cut(s) 418, 896
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 3 cut(s) 154, 160, 510
MvaI CCWGG 3 cut(s) 154, 160, 510
MwoI GCNNNNNNNGC 3 cut(s) 99, 177, 918
NcoI CCATGG 1 cut(s) 334
NdeI CATATG 2 cut(s) 568, 586
NdeII GATC 4 cut(s) 505, 685, 715, 904
NlaIII CATG 8 cut(s) 338, 437, 472, 533, 692, 743, 759, 767
NlaIV GGNNCC 3 cut(s) 61, 426, 816
NmeAIII GCCGAG 2 cut(s) 201, 888
NmuCI GTSAC 3 cut(s) 9, 115, 801
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 2 cut(s) 437, 743
PaeI GCATGC 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 609
PagI TCATGA 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 508
PkrI GCNGC 4 cut(s) 21, 253, 280, 914
PleI GAGTC 2 cut(s) 598, 643
PmaCI CACGTG 1 cut(s) 806
PmlI CACGTG 1 cut(s) 806
PpsI GAGTC 2 cut(s) 598, 643
Ppu21I YACGTR 1 cut(s) 806
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 3 cut(s) 152, 158, 508
PspCI CACGTG 1 cut(s) 806
PspGI CCWGG 3 cut(s) 152, 158, 508
PspN4I GGNNCC 3 cut(s) 61, 426, 816
PspPI GGNCC 3 cut(s) 59, 156, 424
PspPPI RGGWCCY 1 cut(s) 59
PspXI VCTCGAGB 1 cut(s) 609
PstNI CAGNNNCTG 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 4 cut(s) 194, 666, 750, 833
RsaNI GTAC 4 cut(s) 193, 665, 749, 832
SacI GAGCTC 1 cut(s) 663
SatI GCNGC 4 cut(s) 20, 252, 279, 913
Sau3AI GATC 4 cut(s) 505, 685, 715, 904
Sau96I GGNCC 3 cut(s) 59, 156, 424
SchI GAGTC 2 cut(s) 598, 644
ScrFI CCNGG 3 cut(s) 154, 160, 510
SduI GDGCHC 2 cut(s) 149, 663
SexAI ACCWGGT 1 cut(s) 152
SfaNI GCATC 3 cut(s) 588, 633, 752
Sfr274I CTCGAG 1 cut(s) 609
SinI GGWCC 3 cut(s) 59, 156, 424
SlaI CTCGAG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
SphI GCATGC 1 cut(s) 743
Sse9I AATT 3 cut(s) 783, 851, 867
SsiI CCGC 2 cut(s) 64, 75
SspI AATATT 2 cut(s) 550, 596
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 3 cut(s) 152, 158, 508
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 4 cut(s) 43, 70, 213, 703
TaiI ACGT 1 cut(s) 808
TaqI TCGA 2 cut(s) 610, 879
TaqII GACCGA 1 cut(s) 412
TasI AATT 3 cut(s) 783, 851, 867
TatI WGTACW 1 cut(s) 748
TfiI GAWTC 1 cut(s) 164
TseFI GTSAC 3 cut(s) 9, 115, 801
TseI GCWGC 4 cut(s) 19, 251, 278, 912
Tsp45I GTSAC 3 cut(s) 9, 115, 801
TspDTI ATGAA 5 cut(s) 288, 321, 465, 546, 791
VpaK11BI GGWCC 3 cut(s) 59, 156, 424
XapI RAATTY 1 cut(s) 851
XceI RCATGY 2 cut(s) 437, 743
XhoI CTCGAG 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.