Rw1G016690

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005075
Physical Location & Seq
Reverse (-)
37068617 .. 37073782
5166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016690.1

Sequence Viewer

Length: 831 bp
ATGGTTCTGTCACAGAGGCTTCACGAGGCGTTCAAAGGCACCGTCGAGAAAATCACAGGGCCTCGCACCGTCTCCGCTTTCAAAGAGAAAGGCGTCCTCAGCGTCTCCGAGTTCGTCACCGCCGGCGATAACCTCGTCTCCAAGTGCCCTACCTGGTCTTGGGAATCCGGAGAGCCAAGCAAGAGGAAGCCATACCTACCATCTGAAAAGCAGTATTTGATTACTAGAAATGTCCCTTGTTTACGCAGGGCTGCTTGTGTGGAAGAGGAGTATGAAGCTGCAGGTTCGGAAGTTCTTATCGATAATGAAGATAATGATGGCTGGTTGGCAACACACGGGAAGCCAAAGGATAAAAGTAGTGAGGAGGAAAACTTTCCTTCTATTGAAAGTTTAGAAATCAGCAAGGTGAGGTCGGCCCCCTCGCAAGTTGGGGGTGAAGGTGAAGATGAAGATGATATCCCTGACATGGCTGATTATGAAGAAGCTGACAACATTGAAATAGATCCTGGAACATATCTTGTTGCTCATGAACCTGATGATGACAATATTTTACGGACCCGGACATATGATGTCAGCATTACGTATGATAAATATTACCAAACTCCTCGGGTGTGGCTTACTGGCTATGATGAGTCAAGAATGCTTTTACAAGCAGAGCTTGTACTTGAAGATGTCAGTCAAGATCATGCACGCAAAACTGTAACTATTGAGGATCATCCTCACTTGCCCGGAAAGCATGCATCTGTCCATCCATGCCGACATGGGGCTGTGATGAAGAAAATAATTGATGTACTAATGTCACGTGGAGTGGAACCGGAAGTCGATAAGTAA

Protein Analysis

276

Amino Acids

31.03

Weight (kDa)

4.78

Isoelectric Point (pI)

52.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Autophagy_act_C PF03987 37 - 263 3.7e-49 Autophagocytosis associated protein, active-site domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 2 cut(s) 75, 120
AclWI GGATC 2 cut(s) 497, 720
AcvI CACGTG 1 cut(s) 803
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 2 cut(s) 663, 792
AfiI CCNNNNNNNGG 3 cut(s) 159, 466, 763
AgsI TTSAA 5 cut(s) 34, 82, 386, 497, 668
AjnI CCWGG 2 cut(s) 152, 505
AluBI AGCT 3 cut(s) 278, 485, 658
AluI AGCT 3 cut(s) 278, 485, 658
Alw26I GTCTC 3 cut(s) 76, 109, 142
AlwI GGATC 2 cut(s) 497, 720
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 2 cut(s) 59, 414
ApeKI GCWGC 2 cut(s) 251, 278
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
Asp700I GAANNNNTTC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 59, 415, 555
AsuC2I CCSGG 2 cut(s) 559, 729
AsuHPI GGTGA 4 cut(s) 109, 418, 446, 452
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 149
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 23
BbrPI CACGTG 1 cut(s) 803
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 238, 265
BccI CCATC 3 cut(s) 208, 311, 756
BciT130I CCWGG 2 cut(s) 154, 507
BcnI CCSGG 2 cut(s) 559, 729
BcoDI GTCTC 3 cut(s) 76, 109, 142
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 2 cut(s) 252, 279
BlsI GCNGC 2 cut(s) 253, 280
Bme1390I CCNGG 4 cut(s) 154, 507, 559, 729
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 3 cut(s) 59, 415, 555
BmiI GGNNCC 4 cut(s) 40, 417, 557, 813
BmrFI CCNGG 4 cut(s) 154, 507, 559, 729
BmsI GCATC 1 cut(s) 749
Bpu10I CCTNAGC 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 559, 729
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 2 cut(s) 582, 803
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 605
BsaWI WCCGGW 2 cut(s) 167, 814
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 466, 763
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 625
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 2 cut(s) 154, 507
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 605
BseGI GGATG 2 cut(s) 715, 748
BseLI CCNNNNNNNGG 3 cut(s) 159, 466, 763
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 625
BseRI GAGGAG 3 cut(s) 281, 377, 594
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 2 cut(s) 238, 265
BshFI GGCC 2 cut(s) 61, 416
BshNI GGYRCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 5 cut(s) 123, 168, 559, 729, 815
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 3 cut(s) 159, 466, 763
BsmAI GTCTC 3 cut(s) 76, 109, 142
BsmBI CGTCTC 3 cut(s) 76, 109, 142
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 645
BsnI GGCC 2 cut(s) 61, 416
BsoBI CYCGRG 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 3 cut(s) 502, 682, 712
BspACI CCGC 2 cut(s) 75, 120
BspANI GGCC 2 cut(s) 61, 416
BspCNI CTCAG 1 cut(s) 111
BspDI ATCGAT 1 cut(s) 300
BspEI TCCGGA 1 cut(s) 167
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 4 cut(s) 40, 417, 557, 813
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 2 cut(s) 497, 720
BspT107I GGYRCC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 625
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 605
BssMI GATC 3 cut(s) 502, 682, 712
BssNI GRCGYC 1 cut(s) 93
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 154, 507
Bst4CI ACNGT 3 cut(s) 43, 70, 700
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 2 cut(s) 582, 803
BstC8I GCNNGC 3 cut(s) 124, 691, 738
BstDEI CTNAG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 715, 748
BstKTI GATC 3 cut(s) 505, 685, 715
BstMAI GTCTC 3 cut(s) 76, 109, 142
BstMBI GATC 3 cut(s) 502, 682, 712
BstMWI GCNNNNNNNGC 2 cut(s) 99, 733
BstNI CCWGG 2 cut(s) 154, 507
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 4 cut(s) 152, 505, 557, 727
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 582
BstV1I GCAGC 2 cut(s) 238, 265
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 2 cut(s) 61, 416
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 2 cut(s) 715, 748
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 124, 691, 738
CciI TCATGA 1 cut(s) 526
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 59, 415, 555
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 2 cut(s) 82, 91
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 2 cut(s) 662, 791
CviAII CATG 6 cut(s) 466, 527, 686, 737, 753, 761
CviQI GTAC 2 cut(s) 662, 791
DdeI CTNAG 1 cut(s) 98
DpnI GATC 3 cut(s) 504, 684, 714
DpnII GATC 3 cut(s) 502, 682, 712
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 582
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 152, 505
EcoRV GATATC 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 742
Esp3I CGTCTC 3 cut(s) 76, 109, 142
FaeI CATG 6 cut(s) 469, 530, 689, 740, 756, 764
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 465, 526, 685, 736, 752, 760
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 252, 279
FokI GGATG 2 cut(s) 702, 735
Fsp4HI GCNGC 2 cut(s) 252, 279
FspBI CTAG 1 cut(s) 225
GluI GCNGC 2 cut(s) 252, 279
HaeIII GGCC 2 cut(s) 61, 416
HapII CCGG 5 cut(s) 123, 168, 559, 729, 815
HgaI GACGC 2 cut(s) 82, 91
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 6 cut(s) 469, 530, 689, 740, 756, 764
HinfI GANTC 2 cut(s) 164, 632
HpaII CCGG 5 cut(s) 123, 168, 559, 729, 815
HphI GGTGA 4 cut(s) 109, 418, 446, 452
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 3 cut(s) 109, 205, 289
Hpy188III TCNNGA 6 cut(s) 23, 46, 168, 527, 636, 680
Hpy8I GTNNAC 1 cut(s) 242
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 387, 431
HpyCH4III ACNGT 3 cut(s) 43, 70, 700
HpyCH4IV ACGT 2 cut(s) 581, 802
HpyCH4V TGCA 3 cut(s) 281, 689, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 733
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 2 cut(s) 581, 802
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 6 cut(s) 469, 530, 689, 740, 756, 764
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 502, 682, 712
Lsp1109I GCAGC 2 cut(s) 238, 265
LweI GCATC 1 cut(s) 749
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 225
MaeII ACGT 2 cut(s) 581, 802
MaeIII GTNAC 4 cut(s) 9, 115, 700, 798
MalI GATC 3 cut(s) 504, 684, 714
MboI GATC 3 cut(s) 502, 682, 712
MboII GAAGA 7 cut(s) 275, 320, 455, 461, 491, 680, 787
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 783
MlyI GAGTC 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 742
MreI CGCCGGCG 1 cut(s) 122
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 1 cut(s) 372
MspI CCGG 5 cut(s) 123, 168, 559, 729, 815
MspR9I CCNGG 4 cut(s) 154, 507, 559, 729
Mva1269I GAATGC 1 cut(s) 645
MvaI CCWGG 2 cut(s) 154, 507
MwoI GCNNNNNNNGC 2 cut(s) 99, 733
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 2 cut(s) 559, 729
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 502, 682, 712
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 6 cut(s) 469, 530, 689, 740, 756, 764
NlaIV GGNNCC 4 cut(s) 40, 417, 557, 813
NmuCI GTSAC 3 cut(s) 9, 115, 798
NsiI ATGCAT 1 cut(s) 742
NspI RCATGY 1 cut(s) 740
PaeI GCATGC 1 cut(s) 740
PagI TCATGA 1 cut(s) 526
PctI GAATGC 1 cut(s) 645
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 2 cut(s) 253, 280
PleI GAGTC 1 cut(s) 640
PmaCI CACGTG 1 cut(s) 803
PmlI CACGTG 1 cut(s) 803
PpsI GAGTC 1 cut(s) 640
Ppu21I YACGTR 2 cut(s) 582, 803
Psp6I CCWGG 2 cut(s) 152, 505
PspCI CACGTG 1 cut(s) 803
PspGI CCWGG 2 cut(s) 152, 505
PspN4I GGNNCC 4 cut(s) 40, 417, 557, 813
PspPI GGNCC 3 cut(s) 59, 415, 555
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 2 cut(s) 663, 792
RsaNI GTAC 2 cut(s) 662, 791
SatI GCNGC 2 cut(s) 252, 279
Sau3AI GATC 3 cut(s) 502, 682, 712
Sau96I GGNCC 3 cut(s) 59, 415, 555
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 4 cut(s) 154, 507, 559, 729
SduI GDGCHC 1 cut(s) 149
SexAI ACCWGGT 1 cut(s) 152
SfaNI GCATC 1 cut(s) 749
SfcI CTRYAG 1 cut(s) 279
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 1 cut(s) 555
SnaBI TACGTA 1 cut(s) 582
SphI GCATGC 1 cut(s) 740
Sse9I AATT 1 cut(s) 783
SsiI CCGC 2 cut(s) 75, 120
SspI AATATT 2 cut(s) 547, 593
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 152, 505, 557, 727
TaaI ACNGT 3 cut(s) 43, 70, 700
TaiI ACGT 2 cut(s) 584, 805
TaqI TCGA 3 cut(s) 45, 300, 822
TasI AATT 1 cut(s) 783
TatI WGTACW 2 cut(s) 661, 790
TfiI GAWTC 1 cut(s) 164
TseFI GTSAC 3 cut(s) 9, 115, 798
TseI GCWGC 2 cut(s) 251, 278
Tsp45I GTSAC 3 cut(s) 9, 115, 798
TspDTI ATGAA 6 cut(s) 288, 321, 462, 492, 543, 788
TspGWI ACGGA 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 555
XceI RCATGY 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.