FvH4_7g11000

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001812
Physical Location & Seq
Forward (+)
10313170 .. 10317375
4206 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11000.t5

Sequence Viewer

Length: 1380 bp
ATGAGCTCCTGGGACAACTGGGTCGAAGAGTCCCTTCACAAACTCGAACGCCTCCAGCTCCTCCGGTCTCTCCGACCCATCTACCTCTGCCATGACCCACAAACAACCACCACCGTCGAAGACGGCCTCCAAGTCTTTCACGACATGCAGCTCTGGGACCGAGCCTCGGTCGAGGTCCACATTCCCGAAGCAACTTTCCAGAATTGGCTCAGTGACATTCCCAGTTCCGGAGATGAGGAGGATACCCGGAAATTCAGGAAGCTGTTGTTGTTCTCCGGGAACGACTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCAGCTCAAGAACATGGGATGGGCCCAAGGGGGTCTGCTTTGATCTGTGGATATACCGATTACCATAGGCGACTGGAGTCATCCTTAGCAGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGCTTTGCAGCCAATATGGCCTTGATGGTAGTGTTGGGAAATATTGGCTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATTGCCATATTTTCTGATGAACTGAACCATGCATCAATTATTGATGGTATTCGTCTTGCTGAACGACAAAAATCTGTACAGATATTCATCTATAGACATTGTGACATGATTCACCTTAATGCATTGTTATCCAGTTGCACAATGCAGAAGAAAGTTGTTGTGACTGACAGCTTGTTTAGTATGGATGGTGACTTTGCACCAATGATTGAGCTGGTGAAGCTACGCAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTTGTTTGTGGAAAAAATGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAAAGATGTTGACATATGTGTTGGTACTTTGAGCAAGGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCCTTTATATTTTCAACTGCTACACCGTTACCGATTGCTGCTGCGGCTCATGCTGCAGTTATTGTTGCAAGAAAGGAAACATGGCGTAGAAGGGCAATTTGGAGTCGGGTGCAAGATTTTCGTGCTCTGACTGGAATTCCGATAAACAGTCCAATAATATCTCTTATCGTTGGGAGTGAACAGAAGGCTTTGCAAGCAAGCCGGCGTTTGTTGAAATCAGGTTTCCATGTGACTGCAATCAGACCGCCAACAGTGCCACCCAACTCGTGCAGGCTACGAGTGACTTTGAGTGCAACACACACAAGGGCTGATATTGAGAGGTTCACAACTGCACTCTCCAATTGTGTTAATTTCCAAGAAATCGGCATCCATGGCTCCAATGGATATGCAAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

460

Amino Acids

50.79

Weight (kDa)

8.51

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 87 - 345 1.1e-34 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 905
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 3 cut(s) 927, 1025, 1226
AcsI RAATTY 3 cut(s) 251, 860, 1116
AfaI GTAC 2 cut(s) 630, 898
AfiI CCNNNNNNNGG 3 cut(s) 166, 227, 314
AgsI TTSAA 3 cut(s) 430, 996, 1195
AjnI CCWGG 1 cut(s) 8
AjuI GAANNNNNNNTTGG 2 cut(s) 1063, 1095
AluBI AGCT 8 cut(s) 6, 58, 151, 262, 338, 723, 763, 772
AluI AGCT 8 cut(s) 6, 58, 151, 262, 338, 723, 763, 772
Alw21I GWGCWC 2 cut(s) 8, 1108
Alw26I GTCTC 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 3 cut(s) 124, 355, 480
ApaI GGGCCC 1 cut(s) 359
ApoI RAATTY 3 cut(s) 251, 860, 1116
AspS9I GGNCC 4 cut(s) 157, 175, 355, 356
AsuC2I CCSGG 2 cut(s) 247, 277
AsuHPI GGTGA 3 cut(s) 656, 752, 778
AvaII GGWCC 2 cut(s) 157, 175
BaeGI GKGCMC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 8, 359
BauI CACGAG 2 cut(s) 972, 1246
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 8, 1108
BbvCI CCTCAGC 1 cut(s) 855
BccI CCATC 6 cut(s) 86, 346, 481, 590, 731, 945
BceAI ACGGC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 1082, 1116
BciT130I CCWGG 1 cut(s) 10
BciVI GTATCC 1 cut(s) 235
BcnI CCSGG 2 cut(s) 247, 277
BcoDI GTCTC 1 cut(s) 72
BfmI CTRYAG 4 cut(s) 459, 643, 912, 1035
BfuAI ACCTGC 1 cut(s) 905
BfuI GTATCC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 479
Bme1390I CCNGG 3 cut(s) 10, 247, 277
Bme18I GGWCC 2 cut(s) 157, 175
BmgT120I GGNCC 4 cut(s) 157, 175, 355, 356
BmiI GGNNCC 4 cut(s) 158, 357, 820, 1357
BmrFI CCNGG 3 cut(s) 10, 247, 277
BmrI ACTGGG 2 cut(s) 28, 216
BmsI GCATC 3 cut(s) 593, 799, 1356
BmuI ACTGGG 2 cut(s) 28, 216
BoxI GACNNNNGTC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 2 cut(s) 38, 428
Bpu10I CCTNAGC 3 cut(s) 330, 418, 855
BpuEI CTTGAG 2 cut(s) 316, 324
BpuMI CCSGG 2 cut(s) 247, 277
BsaBI GATNNNNATC 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 5 cut(s) 9, 165, 359, 922, 1351
BsaWI WCCGGW 2 cut(s) 63, 227
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 227, 314
Bse118I RCCGGY 1 cut(s) 1182
Bse1I ACTGG 5 cut(s) 23, 222, 411, 684, 1117
Bse3DI GCAATG 1 cut(s) 933
Bse8I GATNNNNATC 1 cut(s) 638
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 5 cut(s) 9, 165, 359, 922, 1351
BseGI GGATG 5 cut(s) 304, 357, 413, 742, 1347
BseJI GATNNNNATC 1 cut(s) 638
BseLI CCNNNNNNNGG 3 cut(s) 166, 227, 314
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 2 cut(s) 223, 846
BseNI ACTGG 5 cut(s) 23, 222, 411, 684, 1117
BseRI GAGGAG 2 cut(s) 50, 251
BseSI GKGCMC 1 cut(s) 359
BsgI GTGCAG 2 cut(s) 1270, 1296
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 3 cut(s) 126, 357, 482
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 8, 1108
BsiSI CCGG 5 cut(s) 64, 228, 247, 276, 1183
BslFI GGGAC 3 cut(s) 16, 26, 170
BslI CCNNNNNNNGG 3 cut(s) 166, 227, 314
BsmAI GTCTC 1 cut(s) 72
BsmFI GGGAC 3 cut(s) 16, 26, 170
BsnI GGCC 3 cut(s) 126, 357, 482
Bso31I GGTCTC 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 355
Bsp1286I GDGCHC 3 cut(s) 8, 359, 1108
Bsp13I TCCGGA 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 1 cut(s) 375
Bsp19I CCATGG 2 cut(s) 922, 1351
BspACI CCGC 3 cut(s) 927, 1025, 1226
BspANI GGCC 3 cut(s) 126, 357, 482
BspCNI CTCAG 2 cut(s) 222, 847
BspEI TCCGGA 1 cut(s) 227
BspLI GGNNCC 4 cut(s) 158, 357, 820, 1357
BspMAI CTGCAG 2 cut(s) 916, 1039
BspMI ACCTGC 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 1182
BsrGI TGTACA 1 cut(s) 628
BsrI ACTGG 5 cut(s) 23, 222, 411, 684, 1117
BssAI RCCGGY 1 cut(s) 1182
BssECI CCNNGG 5 cut(s) 9, 165, 359, 922, 1351
BssMI GATC 1 cut(s) 375
BssSI CACGAG 2 cut(s) 972, 1246
BssT1I CCWWGG 3 cut(s) 359, 922, 1351
Bst2BI CACGAG 2 cut(s) 972, 1246
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 5 cut(s) 115, 969, 1008, 1130, 1234
Bst6I CTCTTC 1 cut(s) 21
BstAUI TGTACA 1 cut(s) 628
BstC8I GCNNGC 6 cut(s) 779, 940, 1176, 1180, 1184, 1253
BstDEI CTNAG 4 cut(s) 209, 330, 418, 855
BstDSI CCRYGG 2 cut(s) 922, 1351
BstF5I GGATG 5 cut(s) 304, 357, 413, 742, 1347
BstKTI GATC 1 cut(s) 378
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 1 cut(s) 375
BstMCI CGRYCG 1 cut(s) 171
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 2 cut(s) 148, 942
BstPAI GACNNNNGTC 1 cut(s) 409
BstSCI CCNGG 3 cut(s) 8, 245, 275
BstSFI CTRYAG 4 cut(s) 459, 643, 912, 1035
BstSLI GKGCMC 1 cut(s) 359
BstV2I GAAGAC 1 cut(s) 126
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 3 cut(s) 126, 357, 482
BtgI CCRYGG 2 cut(s) 922, 1351
BtsCI GGATG 5 cut(s) 304, 357, 413, 742, 1347
BtsIMutI CAGTG 2 cut(s) 217, 1239
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 6 cut(s) 779, 940, 1176, 1180, 1184, 1253
Cfr10I RCCGGY 1 cut(s) 1182
Cfr13I GGNCC 4 cut(s) 157, 175, 355, 356
CseI GACGC 1 cut(s) 987
Csp6I GTAC 2 cut(s) 629, 897
CviQI GTAC 2 cut(s) 629, 897
DdeI CTNAG 4 cut(s) 209, 330, 418, 855
DpnI GATC 1 cut(s) 377
DpnII GATC 1 cut(s) 375
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 359, 922, 1351
Eco24I GRGCYC 2 cut(s) 8, 359
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 157, 175
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 2 cut(s) 860, 1116
EcoRII CCWGG 1 cut(s) 8
EcoT14I CCWWGG 3 cut(s) 359, 922, 1351
EcoT22I ATGCAT 2 cut(s) 586, 676
EcoT38I GRGCYC 2 cut(s) 8, 359
ErhI CCWWGG 3 cut(s) 359, 922, 1351
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 3 cut(s) 16, 26, 170
FauI CCCGC 1 cut(s) 920
FauNDI CATATG 1 cut(s) 887
FokI GGATG 5 cut(s) 291, 364, 400, 749, 1334
FriOI GRGCYC 2 cut(s) 8, 359
GsuI CTGGAG 2 cut(s) 38, 428
HaeIII GGCC 3 cut(s) 126, 357, 482
HapII CCGG 5 cut(s) 64, 228, 247, 276, 1183
HgaI GACGC 1 cut(s) 987
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 4 cut(s) 29, 410, 661, 1084
HpaII CCGG 5 cut(s) 64, 228, 247, 276, 1183
HphI GGTGA 3 cut(s) 656, 752, 778
Hpy166II GTNNAC 5 cut(s) 178, 302, 883, 1160, 1305
Hpy188I TCNGA 5 cut(s) 74, 568, 1110, 1122, 1223
Hpy188III TCNNGA 7 cut(s) 140, 185, 199, 228, 256, 341, 972
Hpy8I GTNNAC 5 cut(s) 178, 302, 883, 1160, 1305
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 4 cut(s) 44, 458, 1065, 1159
HpyCH4III ACNGT 5 cut(s) 115, 969, 1008, 1130, 1234
HpyF3I CTNAG 4 cut(s) 209, 330, 418, 855
Kpn2I TCCGGA 1 cut(s) 227
KroI GCCGGC 1 cut(s) 1182
KroNI GCCGGC 1 cut(s) 1184
Kzo9I GATC 1 cut(s) 375
LmnI GCTCC 4 cut(s) 11, 63, 986, 1361
LweI GCATC 3 cut(s) 593, 799, 1356
MaeIII GTNAC 8 cut(s) 212, 653, 712, 740, 969, 1008, 1210, 1261
MalI GATC 1 cut(s) 377
MboI GATC 1 cut(s) 375
MboII GAAGA 5 cut(s) 38, 131, 442, 445, 712
MfeI CAATTG 1 cut(s) 1321
MhlI GDGCHC 3 cut(s) 8, 359, 1108
MluCI AATT 8 cut(s) 202, 251, 588, 860, 1077, 1116, 1321, 1330
MlyI GAGTC 3 cut(s) 38, 419, 1093
MmeI TCCRAC 1 cut(s) 97
Mph1103I ATGCAT 2 cut(s) 586, 676
MroI TCCGGA 1 cut(s) 227
MroNI GCCGGC 1 cut(s) 1182
MseI TTAA 3 cut(s) 539, 669, 1329
MslI CAYNNNNRTG 1 cut(s) 669
MspI CCGG 5 cut(s) 64, 228, 247, 276, 1183
MspR9I CCNGG 3 cut(s) 10, 247, 277
MunI CAATTG 1 cut(s) 1321
MvaI CCWGG 1 cut(s) 10
NaeI GCCGGC 1 cut(s) 1184
NciI CCSGG 2 cut(s) 247, 277
NcoI CCATGG 2 cut(s) 922, 1351
NdeI CATATG 1 cut(s) 887
NdeII GATC 1 cut(s) 375
NgoMIV GCCGGC 1 cut(s) 1182
NlaIV GGNNCC 4 cut(s) 158, 357, 820, 1357
NmuCI GTSAC 7 cut(s) 212, 653, 712, 740, 969, 1210, 1261
NsiI ATGCAT 2 cut(s) 586, 676
NspI RCATGY 2 cut(s) 148, 942
PaeI GCATGC 1 cut(s) 942
PdiI GCCGGC 1 cut(s) 1184
PfeI GAWTC 1 cut(s) 661
PfoI TCCNGGA 1 cut(s) 275
PleI GAGTC 3 cut(s) 37, 418, 1092
PpsI GAGTC 3 cut(s) 37, 418, 1092
PshAI GACNNNNGTC 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 158, 357, 820, 1357
PspOMI GGGCCC 1 cut(s) 355
PspPI GGNCC 4 cut(s) 157, 175, 355, 356
PstI CTGCAG 2 cut(s) 916, 1039
RsaI GTAC 2 cut(s) 630, 898
RsaNI GTAC 2 cut(s) 629, 897
RseI CAYNNNNRTG 1 cut(s) 669
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 3 cut(s) 539, 669, 1329
Sau3AI GATC 1 cut(s) 375
Sau96I GGNCC 4 cut(s) 157, 175, 355, 356
SchI GAGTC 3 cut(s) 38, 419, 1093
ScrFI CCNGG 3 cut(s) 10, 247, 277
SduI GDGCHC 3 cut(s) 8, 359, 1108
SfaNI GCATC 3 cut(s) 593, 799, 1356
SfcI CTRYAG 4 cut(s) 459, 643, 912, 1035
SinI GGWCC 2 cut(s) 157, 175
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 295, 339
SmoI CTYRAG 2 cut(s) 295, 339
SphI GCATGC 1 cut(s) 942
Sse9I AATT 8 cut(s) 202, 251, 588, 860, 1077, 1116, 1321, 1330
SsiI CCGC 3 cut(s) 927, 1025, 1226
SspI AATATT 1 cut(s) 505
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 8, 245, 275
StyI CCWWGG 3 cut(s) 359, 922, 1351
TaaI ACNGT 5 cut(s) 115, 969, 1008, 1130, 1234
TaqI TCGA 4 cut(s) 24, 45, 117, 171
TaqII GACCGA 2 cut(s) 157, 174
TasI AATT 8 cut(s) 202, 251, 588, 860, 1077, 1116, 1321, 1330
TatI WGTACW 1 cut(s) 628
TauI GCSGC 1 cut(s) 1028
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 3 cut(s) 539, 669, 1329
Tru9I TTAA 3 cut(s) 539, 669, 1329
TscAI CASTG 2 cut(s) 217, 1239
TseFI GTSAC 7 cut(s) 212, 653, 712, 740, 969, 1210, 1261
Tsp45I GTSAC 7 cut(s) 212, 653, 712, 740, 969, 1210, 1261
TspDTI ATGAA 4 cut(s) 561, 585, 628, 922
TspRI CASTG 2 cut(s) 217, 1239
VpaK11BI GGWCC 2 cut(s) 157, 175
XapI RAATTY 3 cut(s) 251, 860, 1116
XceI RCATGY 2 cut(s) 148, 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 1358
Zsp2I ATGCAT 2 cut(s) 586, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.