Rroxscaffold_4G00306440

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001812
Physical Location & Seq
Reverse (-)
27326025 .. 27328434
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306440.1

Sequence Viewer

Length: 1086 bp
ATGAGCTCCTGGGACAACTGGGTCGAACACTCCCTTAACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACCTCCGCAATGAACCACAAAAACCCACCACCACCGTCGAAGATGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCCGGGGATGAGGAGGATACCCGGAAATTTAAAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGCTCTGCTTTGATCTGTGGATATACCGATTACCATAGGCGACTGGAGTCATCCTTAGCAGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCTTGATGGTAGTGTTGGGAAATGTTGGCTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATTGCCATTTTTTCTGATGAACTGAACCATGCGTCGATAATTGATGGTATTCGTCTTGCAGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGATTCACCTTAAGGCATTGTTATCCAGTTGCACAATGCAGAAGAAAGTTGTTGTGACTGATAGCTTGTTTAGTATGGATGGAGACTTTGCACCAATGATCGAGCTGGTGAAGCTACGCAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTTGTTTGTGGTAAAAATGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAGAGATGTTGACATATGTGTTGGCACGTTGAGTAAGGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGGGTAGATCTTCAAAGAATTAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCCTTTATATTTTCAACTGCTACACCATTACCAATTGCTGCTGCAGCCCATGGTAAATTATATGAATTTGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

361

Amino Acids

40.24

Weight (kDa)

6.7

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 89 - 354 9.4e-33 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 137
Acc36I ACCTGC 1 cut(s) 911
AciI CCGC 3 cut(s) 88, 341, 933
AclI AACGTT 1 cut(s) 200
AcsI RAATTY 3 cut(s) 257, 866, 1073
AfiI CCNNNNNNNGG 3 cut(s) 233, 234, 320
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 4 cut(s) 47, 436, 962, 1023
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 6 cut(s) 6, 157, 268, 729, 769, 778
AluI AGCT 6 cut(s) 6, 157, 268, 729, 769, 778
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 2 cut(s) 72, 741
AoxI GGCC 2 cut(s) 361, 486
ApaI GGGCCC 1 cut(s) 365
ApoI RAATTY 3 cut(s) 257, 866, 1073
AspS9I GGNCC 4 cut(s) 163, 181, 361, 362
AsuC2I CCSGG 2 cut(s) 235, 253
AsuHPI GGTGA 2 cut(s) 662, 784
AvaII GGWCC 2 cut(s) 163, 181
BaeGI GKGCMC 1 cut(s) 365
BanII GRGCYC 3 cut(s) 8, 365, 374
BauI CACGAG 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 861
BccI CCATC 5 cut(s) 86, 487, 596, 737, 972
BciT130I CCWGG 1 cut(s) 10
BciVI GTATCC 1 cut(s) 241
BcnI CCSGG 2 cut(s) 235, 253
BcoDI GTCTC 2 cut(s) 72, 741
BfmI CTRYAG 5 cut(s) 465, 633, 649, 918, 1050
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 911
BfuI GTATCC 1 cut(s) 241
BglI GCCNNNNNGGC 2 cut(s) 369, 485
BglII AGATCT 1 cut(s) 955
Bme1390I CCNGG 3 cut(s) 10, 235, 253
Bme18I GGWCC 2 cut(s) 163, 181
BmgT120I GGNCC 4 cut(s) 163, 181, 361, 362
BmiI GGNNCC 3 cut(s) 164, 363, 826
BmrFI CCNGG 3 cut(s) 10, 235, 253
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 1 cut(s) 805
BmuI ACTGGG 2 cut(s) 28, 222
BoxI GACNNNNGTC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 1 cut(s) 434
Bpu10I CCTNAGC 3 cut(s) 336, 424, 861
BpuEI CTTGAG 2 cut(s) 322, 330
BpuMI CCSGG 2 cut(s) 235, 253
BsaBI GATNNNNATC 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 6 cut(s) 9, 174, 234, 365, 928, 1057
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 234, 320
Bse1I ACTGG 4 cut(s) 23, 228, 417, 690
Bse3DI GCAATG 2 cut(s) 97, 939
Bse8I GATNNNNATC 1 cut(s) 644
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 6 cut(s) 9, 174, 234, 365, 928, 1057
BseGI GGATG 5 cut(s) 222, 244, 310, 419, 748
BseJI GATNNNNATC 1 cut(s) 644
BseLI CCNNNNNNNGG 3 cut(s) 233, 234, 320
BseMI GCAATG 2 cut(s) 97, 939
BseMII CTCAG 2 cut(s) 229, 852
BseNI ACTGG 4 cut(s) 23, 228, 417, 690
BseRI GAGGAG 2 cut(s) 50, 257
BseSI GKGCMC 1 cut(s) 365
BshFI GGCC 2 cut(s) 363, 488
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 3 cut(s) 64, 234, 253
BslFI GGGAC 2 cut(s) 26, 176
BslI CCNNNNNNNGG 3 cut(s) 233, 234, 320
BsmAI GTCTC 2 cut(s) 72, 741
BsmFI GGGAC 2 cut(s) 26, 176
BsnI GGCC 2 cut(s) 363, 488
Bso31I GGTCTC 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 8, 365, 374
Bsp143I GATC 3 cut(s) 381, 762, 955
Bsp19I CCATGG 2 cut(s) 928, 1057
BspACI CCGC 3 cut(s) 88, 341, 933
BspANI GGCC 2 cut(s) 363, 488
BspCNI CTCAG 2 cut(s) 228, 853
BspLI GGNNCC 3 cut(s) 164, 363, 826
BspMAI CTGCAG 2 cut(s) 922, 1054
BspMI ACCTGC 1 cut(s) 911
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 97, 939
BsrI ACTGG 4 cut(s) 23, 228, 417, 690
BssECI CCNNGG 6 cut(s) 9, 174, 234, 365, 928, 1057
BssMI GATC 3 cut(s) 381, 762, 955
BssSI CACGAG 1 cut(s) 999
BssT1I CCWWGG 3 cut(s) 365, 928, 1057
Bst2BI CACGAG 1 cut(s) 999
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 118, 167, 996
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 3 cut(s) 53, 785, 946
BstDEI CTNAG 4 cut(s) 215, 336, 424, 861
BstDSI CCRYGG 3 cut(s) 174, 928, 1057
BstF5I GGATG 5 cut(s) 222, 244, 310, 419, 748
BstKTI GATC 3 cut(s) 384, 765, 958
BstMAI GTCTC 2 cut(s) 72, 741
BstMBI GATC 3 cut(s) 381, 762, 955
BstMWI GCNNNNNNNGC 7 cut(s) 338, 341, 369, 485, 775, 784, 1052
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 948
BstPAI GACNNNNGTC 1 cut(s) 415
BstSCI CCNGG 3 cut(s) 8, 233, 251
BstSFI CTRYAG 5 cut(s) 465, 633, 649, 918, 1050
BstSLI GKGCMC 1 cut(s) 365
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 363, 488
BtgI CCRYGG 3 cut(s) 174, 928, 1057
BtsCI GGATG 5 cut(s) 222, 244, 310, 419, 748
BtsIMutI CAGTG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 3 cut(s) 53, 785, 946
Cfr13I GGNCC 4 cut(s) 163, 181, 361, 362
CseI GACGC 2 cut(s) 579, 1014
CviAII CATG 8 cut(s) 353, 587, 664, 791, 821, 929, 945, 1058
DdeI CTNAG 4 cut(s) 215, 336, 424, 861
DpnI GATC 3 cut(s) 383, 764, 957
DpnII GATC 3 cut(s) 381, 762, 955
DraI TTTAAA 1 cut(s) 262
DrdI GACNNNNNNGTC 2 cut(s) 20, 137
DseDI GACNNNNNNGTC 2 cut(s) 20, 137
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 365, 928, 1057
Eco24I GRGCYC 3 cut(s) 8, 365, 374
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 163, 181
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 1 cut(s) 8
EcoT14I CCWWGG 3 cut(s) 365, 928, 1057
EcoT38I GRGCYC 3 cut(s) 8, 365, 374
ErhI CCWWGG 3 cut(s) 365, 928, 1057
FaeI CATG 8 cut(s) 356, 590, 667, 794, 824, 932, 948, 1061
FaqI GGGAC 2 cut(s) 26, 176
FatI CATG 8 cut(s) 352, 586, 663, 790, 820, 928, 944, 1057
FauI CCCGC 1 cut(s) 926
FauNDI CATATG 1 cut(s) 893
FokI GGATG 5 cut(s) 209, 251, 297, 406, 755
FriOI GRGCYC 3 cut(s) 8, 365, 374
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 2 cut(s) 363, 488
HapII CCGG 3 cut(s) 64, 234, 253
HgaI GACGC 2 cut(s) 579, 1014
Hin1II CATG 8 cut(s) 356, 590, 667, 794, 824, 932, 948, 1061
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HinfI GANTC 3 cut(s) 194, 416, 667
HpaII CCGG 3 cut(s) 64, 234, 253
HphI GGTGA 2 cut(s) 662, 784
Hpy166II GTNNAC 3 cut(s) 184, 308, 889
Hpy188I TCNGA 2 cut(s) 74, 574
Hpy188III TCNNGA 5 cut(s) 191, 205, 282, 347, 999
Hpy8I GTNNAC 3 cut(s) 184, 308, 889
Hpy99I CGWCG 2 cut(s) 122, 595
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 3 cut(s) 118, 167, 996
HpyCH4IV ACGT 2 cut(s) 200, 905
HpyF10VI GCNNNNNNNGC 7 cut(s) 338, 341, 369, 485, 775, 784, 1052
HpyF3I CTNAG 4 cut(s) 215, 336, 424, 861
HpySE526I ACGT 2 cut(s) 200, 905
Hsp92II CATG 8 cut(s) 356, 590, 667, 794, 824, 932, 948, 1061
Kzo9I GATC 3 cut(s) 381, 762, 955
LmnI GCTCC 2 cut(s) 11, 1013
LweI GCATC 1 cut(s) 805
MaeII ACGT 2 cut(s) 200, 905
MaeIII GTNAC 4 cut(s) 218, 659, 718, 996
MalI GATC 3 cut(s) 383, 764, 957
MboI GATC 3 cut(s) 381, 762, 955
MboII GAAGA 6 cut(s) 133, 134, 448, 451, 718, 950
MfeI CAATTG 1 cut(s) 1041
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 3 cut(s) 8, 365, 374
MluCI AATT 7 cut(s) 257, 597, 866, 966, 1041, 1064, 1073
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 97
MseI TTAA 5 cut(s) 36, 261, 545, 675, 1084
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 3 cut(s) 64, 234, 253
MspR9I CCNGG 3 cut(s) 10, 235, 253
MunI CAATTG 1 cut(s) 1041
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 7 cut(s) 338, 341, 369, 485, 775, 784, 1052
NciI CCSGG 2 cut(s) 235, 253
NcoI CCATGG 2 cut(s) 928, 1057
NdeI CATATG 1 cut(s) 893
NdeII GATC 3 cut(s) 381, 762, 955
NlaIII CATG 8 cut(s) 356, 590, 667, 794, 824, 932, 948, 1061
NlaIV GGNNCC 3 cut(s) 164, 363, 826
NmuCI GTSAC 4 cut(s) 218, 659, 718, 996
NspI RCATGY 1 cut(s) 948
PaeI GCATGC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 194, 667
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
PshAI GACNNNNGTC 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 8
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 164, 363, 826
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 4 cut(s) 163, 181, 361, 362
PstI CTGCAG 2 cut(s) 922, 1054
PsuI RGATCY 1 cut(s) 955
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 5 cut(s) 36, 261, 545, 675, 1084
Sau3AI GATC 3 cut(s) 381, 762, 955
Sau96I GGNCC 4 cut(s) 163, 181, 361, 362
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 3 cut(s) 10, 235, 253
SduI GDGCHC 3 cut(s) 8, 365, 374
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 5 cut(s) 465, 633, 649, 918, 1050
SinI GGWCC 2 cut(s) 163, 181
SmlI CTYRAG 3 cut(s) 301, 345, 674
SmoI CTYRAG 3 cut(s) 301, 345, 674
SphI GCATGC 1 cut(s) 948
Sse9I AATT 7 cut(s) 257, 597, 866, 966, 1041, 1064, 1073
SsiI CCGC 3 cut(s) 88, 341, 933
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 8, 233, 251
StyI CCWWGG 3 cut(s) 365, 928, 1057
TaaI ACNGT 3 cut(s) 118, 167, 996
TaiI ACGT 2 cut(s) 203, 908
TaqI TCGA 4 cut(s) 24, 120, 593, 765
TasI AATT 7 cut(s) 257, 597, 866, 966, 1041, 1064, 1073
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 194, 667
Tru1I TTAA 5 cut(s) 36, 261, 545, 675, 1084
Tru9I TTAA 5 cut(s) 36, 261, 545, 675, 1084
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 4 cut(s) 218, 659, 718, 996
Tsp45I GTSAC 4 cut(s) 218, 659, 718, 996
TspDTI ATGAA 6 cut(s) 108, 567, 591, 634, 928, 1086
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 1 cut(s) 223
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 163, 181
XapI RAATTY 3 cut(s) 257, 866, 1073
XceI RCATGY 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.