Rh6AG012000

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001812
Physical Location & Seq
Reverse (-)
1252306 .. 1255027
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG012000.1

Sequence Viewer

Length: 798 bp
ATGGGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACTTCCGCAATGAACCTCAAAAACCCACCACCACCATCGAAGATGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCTGGAGATGAGGAAGATACCCGGAGATTCAGAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGCTCTGCTTTGATCTGTGGATATACAGATTACCATAGGCGACTGGAGTCATCCTTAGCGGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCTTGATGAACAAGAAAGAAAGAAAGAAGGTGAGGGAGGTGGGGATCTTGAAGAAGGATCCTGAGACCCTGAAGGAGCAGATTGATAAGTTGAATAAGCAGAGAGATGAAATGACTCGAATTTTCTGGAAATCTATAAAAGATAAGCTTATTTTGCCCTTTCTGGAACTGGATATCAAGTACTTTGATCTTGGCCTCCCTAATCGTGATGCCACCAGTGATAGAGTCACAATTGAAAGTGCAGAAGCTACTCTTAAGTATGCCTTATCATTCTGCCTTCATGTTACCTTTGCTAGACTACAGTACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

265

Amino Acids

30.67

Weight (kDa)

7.05

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Wbp11 PF09429 166 - 197 3.8e-06 WW domain binding protein 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 137
AciI CCGC 3 cut(s) 88, 341, 428
AclI AACGTT 1 cut(s) 200
AclWI GGATC 3 cut(s) 539, 539, 552
AcsI RAATTY 1 cut(s) 606
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 2 cut(s) 668, 791
AfiI CCNNNNNNNGG 2 cut(s) 233, 320
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 5 cut(s) 47, 436, 538, 580, 722
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 4 cut(s) 157, 268, 634, 734
AluI AGCT 4 cut(s) 157, 268, 634, 734
Alw26I GTCTC 2 cut(s) 72, 545
AlwI GGATC 3 cut(s) 539, 539, 552
AoxI GGCC 3 cut(s) 361, 486, 679
ApaI GGGCCC 1 cut(s) 365
ApeKI GCWGC 4 cut(s) 154, 329, 332, 476
ApoI RAATTY 1 cut(s) 606
AspS9I GGNCC 4 cut(s) 163, 181, 361, 362
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 529
AvaII GGWCC 2 cut(s) 163, 181
BaeGI GKGCMC 1 cut(s) 365
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 3 cut(s) 8, 365, 374
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 4 cut(s) 166, 316, 319, 488
BccI CCATC 2 cut(s) 86, 125
BciT130I CCWGG 1 cut(s) 10
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 72, 545
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 2 cut(s) 465, 785
BfrI CTTAAG 1 cut(s) 740
BglI GCCNNNNNGGC 2 cut(s) 369, 485
BisI GCNGC 5 cut(s) 155, 330, 333, 342, 477
BlsI GCNGC 5 cut(s) 156, 331, 334, 343, 478
BmcAI AGTACT 2 cut(s) 668, 791
Bme1390I CCNGG 2 cut(s) 10, 253
Bme18I GGWCC 2 cut(s) 163, 181
BmgT120I GGNCC 4 cut(s) 163, 181, 361, 362
BmiI GGNNCC 4 cut(s) 7, 164, 363, 546
BmrFI CCNGG 2 cut(s) 10, 253
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 1 cut(s) 685
BmuI ACTGGG 2 cut(s) 28, 222
BoxI GACNNNNGTC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 2 cut(s) 255, 434
Bpu10I CCTNAGC 2 cut(s) 336, 424
BpuEI CTTGAG 2 cut(s) 322, 330
BpuMI CCSGG 1 cut(s) 253
BsaI GGTCTC 2 cut(s) 72, 545
BsaJI CCNNGG 3 cut(s) 9, 174, 365
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 320
Bse1I ACTGG 5 cut(s) 23, 228, 417, 660, 702
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 3 cut(s) 9, 174, 365
BseGI GGATG 3 cut(s) 222, 310, 419
BseLI CCNNNNNNNGG 2 cut(s) 233, 320
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 229, 540
BseNI ACTGG 5 cut(s) 23, 228, 417, 660, 702
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 4 cut(s) 166, 316, 319, 488
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 3 cut(s) 363, 488, 681
BsiSI CCGG 2 cut(s) 64, 253
BslFI GGGAC 2 cut(s) 26, 176
BslI CCNNNNNNNGG 2 cut(s) 233, 320
BsmAI GTCTC 2 cut(s) 72, 545
BsmFI GGGAC 2 cut(s) 26, 176
BsnI GGCC 3 cut(s) 363, 488, 681
Bso31I GGTCTC 2 cut(s) 72, 545
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 8, 365, 374
Bsp143I GATC 4 cut(s) 381, 531, 544, 673
BspACI CCGC 3 cut(s) 88, 341, 428
BspANI GGCC 3 cut(s) 363, 488, 681
BspCNI CTCAG 2 cut(s) 228, 541
BspLI GGNNCC 4 cut(s) 7, 164, 363, 546
BspPI GGATC 3 cut(s) 539, 539, 552
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 2 cut(s) 72, 545
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 5 cut(s) 23, 228, 417, 660, 702
BssECI CCNNGG 3 cut(s) 9, 174, 365
BssMI GATC 4 cut(s) 381, 531, 544, 673
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 167, 789, 794
BstAFI CTTAAG 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 4 cut(s) 215, 336, 424, 549
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 3 cut(s) 222, 310, 419
BstKTI GATC 4 cut(s) 384, 534, 547, 676
BstMAI GTCTC 2 cut(s) 72, 545
BstMBI GATC 4 cut(s) 381, 531, 544, 673
BstMWI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
BstNI CCWGG 1 cut(s) 10
BstPAI GACNNNNGTC 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 8, 251
BstSFI CTRYAG 2 cut(s) 465, 785
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 4 cut(s) 166, 316, 319, 488
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 2 cut(s) 531, 544
BstYI RGATCY 2 cut(s) 531, 544
BsuRI GGCC 3 cut(s) 363, 488, 681
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 3 cut(s) 222, 310, 419
BtsIMutI CAGTG 2 cut(s) 223, 709
Cac8I GCNNGC 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 163, 181, 361, 362
Csp6I GTAC 2 cut(s) 667, 790
CviAII CATG 2 cut(s) 353, 767
CviQI GTAC 2 cut(s) 667, 790
DdeI CTNAG 4 cut(s) 215, 336, 424, 549
DpnI GATC 4 cut(s) 383, 533, 546, 675
DpnII GATC 4 cut(s) 381, 531, 544, 673
DrdI GACNNNNNNGTC 2 cut(s) 20, 137
DseDI GACNNNNNNGTC 2 cut(s) 20, 137
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 3 cut(s) 8, 365, 374
Eco31I GGTCTC 2 cut(s) 72, 545
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 163, 181
Eco57I CTGAAG 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 8
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 3 cut(s) 8, 365, 374
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 2 cut(s) 356, 770
FaiI YATR 7 cut(s) 354, 393, 405, 485, 623, 747, 768
FalI AAGNNNNNCTT 6 cut(s) 618, 650, 723, 755, 734, 766
FaqI GGGAC 2 cut(s) 26, 176
FatI CATG 2 cut(s) 352, 766
Fnu4HI GCNGC 5 cut(s) 155, 330, 333, 342, 477
FokI GGATG 3 cut(s) 209, 297, 406
FriOI GRGCYC 3 cut(s) 8, 365, 374
Fsp4HI GCNGC 5 cut(s) 155, 330, 333, 342, 477
FspBI CTAG 1 cut(s) 780
GluI GCNGC 5 cut(s) 155, 330, 333, 342, 477
GsuI CTGGAG 2 cut(s) 255, 434
HaeIII GGCC 3 cut(s) 363, 488, 681
HapII CCGG 2 cut(s) 64, 253
Hin1II CATG 2 cut(s) 356, 770
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 194, 258, 416, 601, 711
HpaII CCGG 2 cut(s) 64, 253
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 2 cut(s) 184, 308
Hpy188I TCNGA 2 cut(s) 74, 263
Hpy8I GTNNAC 2 cut(s) 184, 308
HpyAV CCTTC 5 cut(s) 464, 508, 535, 553, 773
HpyCH4III ACNGT 3 cut(s) 167, 789, 794
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 55, 154, 476, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
HpyF3I CTNAG 4 cut(s) 215, 336, 424, 549
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 2 cut(s) 356, 770
Kzo9I GATC 4 cut(s) 381, 531, 544, 673
LmnI GCTCC 2 cut(s) 11, 562
Lsp1109I GCAGC 4 cut(s) 166, 316, 319, 488
LweI GCATC 1 cut(s) 685
MaeI CTAG 1 cut(s) 780
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 218, 712, 769
MalI GATC 4 cut(s) 383, 533, 546, 675
MboI GATC 4 cut(s) 381, 531, 544, 673
MboII GAAGA 6 cut(s) 133, 134, 257, 448, 451, 550
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 531, 544
MhlI GDGCHC 3 cut(s) 8, 365, 374
MluCI AATT 2 cut(s) 606, 717
MlyI GAGTC 3 cut(s) 425, 595, 720
MmeI TCCRAC 1 cut(s) 97
MseI TTAA 1 cut(s) 741
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 2 cut(s) 64, 253
MspR9I CCNGG 2 cut(s) 10, 253
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 381, 531, 544, 673
NlaIII CATG 2 cut(s) 356, 770
NlaIV GGNNCC 4 cut(s) 7, 164, 363, 546
NmuCI GTSAC 2 cut(s) 218, 712
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 194, 258
PkrI GCNGC 5 cut(s) 156, 331, 334, 343, 478
PleI GAGTC 3 cut(s) 424, 595, 719
PpsI GAGTC 3 cut(s) 424, 595, 719
PshAI GACNNNNGTC 1 cut(s) 415
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 7, 164, 363, 546
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 4 cut(s) 163, 181, 361, 362
PsuI RGATCY 2 cut(s) 531, 544
RsaI GTAC 2 cut(s) 668, 791
RsaNI GTAC 2 cut(s) 667, 790
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 5 cut(s) 155, 330, 333, 342, 477
Sau3AI GATC 4 cut(s) 381, 531, 544, 673
Sau96I GGNCC 4 cut(s) 163, 181, 361, 362
ScaI AGTACT 2 cut(s) 668, 791
SchI GAGTC 3 cut(s) 425, 595, 720
ScrFI CCNGG 2 cut(s) 10, 253
SduI GDGCHC 3 cut(s) 8, 365, 374
SfaNI GCATC 1 cut(s) 685
SfcI CTRYAG 2 cut(s) 465, 785
SinI GGWCC 2 cut(s) 163, 181
SmlI CTYRAG 3 cut(s) 301, 345, 740
SmoI CTYRAG 3 cut(s) 301, 345, 740
Sse9I AATT 2 cut(s) 606, 717
SsiI CCGC 3 cut(s) 88, 341, 428
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 8, 251
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 167, 789, 794
TaiI ACGT 1 cut(s) 203
TaqI TCGA 3 cut(s) 24, 120, 604
TasI AATT 2 cut(s) 606, 717
TatI WGTACW 2 cut(s) 666, 789
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 194, 258
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 2 cut(s) 223, 709
TseFI GTSAC 2 cut(s) 218, 712
TseI GCWGC 4 cut(s) 154, 329, 332, 476
Tsp45I GTSAC 2 cut(s) 218, 712
TspDTI ATGAA 4 cut(s) 108, 509, 609, 755
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 2 cut(s) 223, 709
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 163, 181
XapI RAATTY 1 cut(s) 606
XspI CTAG 1 cut(s) 780
ZrmI AGTACT 2 cut(s) 668, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.