Rh1DG212200

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001812
Physical Location & Seq
Forward (+)
41258211 .. 41263254
5044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG212200.1

Sequence Viewer

Length: 1272 bp
ATGCCAAGCACGAGAAATGAACGACAGTCGGTGCTTCGAGAAATATCTACCATGATTCCATGTTACCAAAAGTCGAGCAACCAACATATGAGAGGTAGCGACATGTCGTTTTCCCTATGTACTGTTACATGGGAAGCCGAACCTTCACACTCTCCAACAATCCAGCTACCCCCGGCGGCTTCTCAGTCTCTCTGCAAATCAAACACGATGAGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTTCGACCCATCTACCTCCGCAATGAACCACAAAAACCCACCACCACCGTCGAAGACGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCCGGGGATGAGGAGGATACCCGGAAATTTAGAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGGTCTGCTTTGATCTGTGGATATACCGATTACCATAGGCGACTGGAGTCATCCTTAGCAGCCTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCCTGATGGTAGTGCTGGGAAATGTCGGCTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATAGCCATTTTTTCTGATGAACTGAACCATGCGTCAATAATTGATGGTATTCGTCTTGCTGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGATTCACCTTAAGGCATTGCTATCCAGTTGCACAATGCAGAAGAAAGTTGTTGTGACTGATTGCTTGTTTAGTATGGATGGAGACTTCGCACCAATGATCGAGCTGGTGAAACTACGCAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTTGTTTGCGGAAAAAATGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAGAGATGTTGACATATGTGTTGGCACTTTGAGTAAGGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCCTTTATATTTTCAACTGCTACACCATTACCAATTGCTGCTGCGGCCCATGGTAAATTATATGAATTTGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

423

Amino Acids

47.04

Weight (kDa)

6.76

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 158 - 416 9.1e-35 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 227, 344
Acc36I ACCTGC 1 cut(s) 1118
AciI CCGC 6 cut(s) 176, 295, 548, 1044, 1140, 1238
AclI AACGTT 1 cut(s) 407
AcsI RAATTY 3 cut(s) 464, 1073, 1259
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 3 cut(s) 440, 441, 527
AflII CTTAAG 1 cut(s) 881
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 3 cut(s) 254, 643, 1209
AjnI CCWGG 1 cut(s) 215
AluBI AGCT 5 cut(s) 166, 213, 364, 475, 976
AluI AGCT 5 cut(s) 166, 213, 364, 475, 976
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 3 cut(s) 192, 279, 948
AoxI GGCC 3 cut(s) 568, 693, 1239
ApaI GGGCCC 1 cut(s) 572
ApoI RAATTY 3 cut(s) 464, 1073, 1259
AspS9I GGNCC 6 cut(s) 370, 388, 568, 569, 694, 1240
AsuC2I CCSGG 3 cut(s) 173, 442, 460
AsuHPI GGTGA 2 cut(s) 869, 991
AvaII GGWCC 2 cut(s) 370, 388
BaeGI GKGCMC 1 cut(s) 572
BanII GRGCYC 2 cut(s) 215, 572
BauI CACGAG 2 cut(s) 10, 1185
BbsI GAAGAC 2 cut(s) 336, 340
Bbv12I GWGCWC 1 cut(s) 215
BbvCI CCTCAGC 1 cut(s) 1068
BccI CCATC 5 cut(s) 293, 694, 803, 944, 1158
BciT130I CCWGG 1 cut(s) 217
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 3 cut(s) 173, 442, 460
BcoDI GTCTC 3 cut(s) 192, 279, 948
BfmI CTRYAG 4 cut(s) 672, 840, 856, 1125
BfrI CTTAAG 1 cut(s) 881
BfuAI ACCTGC 1 cut(s) 1118
BfuI GTATCC 1 cut(s) 448
BglI GCCNNNNNGGC 1 cut(s) 692
Bme1390I CCNGG 4 cut(s) 173, 217, 442, 460
Bme18I GGWCC 2 cut(s) 370, 388
BmgT120I GGNCC 6 cut(s) 370, 388, 568, 569, 694, 1240
BmiI GGNNCC 3 cut(s) 371, 570, 1033
BmrFI CCNGG 4 cut(s) 173, 217, 442, 460
BmrI ACTGGG 2 cut(s) 235, 429
BmsI GCATC 1 cut(s) 1012
BmuI ACTGGG 2 cut(s) 235, 429
BoxI GACNNNNGTC 1 cut(s) 622
BpiI GAAGAC 2 cut(s) 336, 340
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 3 cut(s) 543, 631, 1068
BpuEI CTTGAG 2 cut(s) 529, 537
BpuMI CCSGG 3 cut(s) 173, 442, 460
BsaBI GATNNNNATC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 7 cut(s) 171, 216, 381, 441, 572, 1135, 1243
BsaWI WCCGGW 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 441, 527
Bse1I ACTGG 4 cut(s) 230, 435, 624, 897
Bse3DI GCAATG 3 cut(s) 304, 887, 1146
Bse8I GATNNNNATC 1 cut(s) 851
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 7 cut(s) 171, 216, 381, 441, 572, 1135, 1243
BseGI GGATG 5 cut(s) 429, 451, 517, 626, 955
BseJI GATNNNNATC 1 cut(s) 851
BseLI CCNNNNNNNGG 3 cut(s) 440, 441, 527
BseMI GCAATG 3 cut(s) 304, 887, 1146
BseMII CTCAG 3 cut(s) 197, 436, 1059
BseNI ACTGG 4 cut(s) 230, 435, 624, 897
BseRI GAGGAG 2 cut(s) 257, 464
BseSI GKGCMC 1 cut(s) 572
BseYI CCCAGC 1 cut(s) 709
BshFI GGCC 3 cut(s) 570, 695, 1241
BsiHKAI GWGCWC 1 cut(s) 215
BsiSI CCGG 4 cut(s) 173, 271, 441, 460
BslFI GGGAC 2 cut(s) 233, 383
BslI CCNNNNNNNGG 3 cut(s) 440, 441, 527
BsmAI GTCTC 3 cut(s) 192, 279, 948
BsmFI GGGAC 2 cut(s) 233, 383
BsnI GGCC 3 cut(s) 570, 695, 1241
Bso31I GGTCTC 1 cut(s) 279
Bsp120I GGGCCC 1 cut(s) 568
Bsp1286I GDGCHC 2 cut(s) 215, 572
Bsp143I GATC 2 cut(s) 588, 969
Bsp19I CCATGG 2 cut(s) 1135, 1243
BspACI CCGC 6 cut(s) 176, 295, 548, 1044, 1140, 1238
BspANI GGCC 3 cut(s) 570, 695, 1241
BspCNI CTCAG 3 cut(s) 196, 435, 1060
BspLI GGNNCC 3 cut(s) 371, 570, 1033
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 1 cut(s) 1118
BspTI CTTAAG 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 3 cut(s) 304, 887, 1146
BsrI ACTGG 4 cut(s) 230, 435, 624, 897
BssECI CCNNGG 7 cut(s) 171, 216, 381, 441, 572, 1135, 1243
BssMI GATC 2 cut(s) 588, 969
BssSI CACGAG 2 cut(s) 10, 1185
BssT1I CCWWGG 3 cut(s) 572, 1135, 1243
Bst2BI CACGAG 2 cut(s) 10, 1185
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 5 cut(s) 27, 124, 325, 374, 1182
Bst6I CTCTTC 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 881
BstC8I GCNNGC 3 cut(s) 260, 992, 1153
BstDEI CTNAG 5 cut(s) 183, 422, 543, 631, 1068
BstDSI CCRYGG 3 cut(s) 381, 1135, 1243
BstF5I GGATG 5 cut(s) 429, 451, 517, 626, 955
BstKTI GATC 2 cut(s) 591, 972
BstMAI GTCTC 3 cut(s) 192, 279, 948
BstMBI GATC 2 cut(s) 588, 969
BstMWI GCNNNNNNNGC 4 cut(s) 545, 548, 692, 1238
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 2 cut(s) 106, 1155
BstPAI GACNNNNGTC 1 cut(s) 622
BstSCI CCNGG 4 cut(s) 171, 215, 440, 458
BstSFI CTRYAG 4 cut(s) 672, 840, 856, 1125
BstSLI GKGCMC 1 cut(s) 572
BstV2I GAAGAC 2 cut(s) 336, 340
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 3 cut(s) 570, 695, 1241
BtgI CCRYGG 3 cut(s) 381, 1135, 1243
BtsCI GGATG 5 cut(s) 429, 451, 517, 626, 955
BtsIMutI CAGTG 1 cut(s) 430
BveI ACCTGC 1 cut(s) 1118
Cac8I GCNNGC 3 cut(s) 260, 992, 1153
Cfr13I GGNCC 6 cut(s) 370, 388, 568, 569, 694, 1240
CseI GACGC 2 cut(s) 786, 1200
Csp6I GTAC 1 cut(s) 120
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 5 cut(s) 183, 422, 543, 631, 1068
DpnI GATC 2 cut(s) 590, 971
DpnII GATC 2 cut(s) 588, 969
DrdI GACNNNNNNGTC 2 cut(s) 227, 344
DseDI GACNNNNNNGTC 2 cut(s) 227, 344
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 3 cut(s) 572, 1135, 1243
Eco24I GRGCYC 2 cut(s) 215, 572
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 370, 388
Eco53kI GAGCTC 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 213
EcoRI GAATTC 1 cut(s) 1073
EcoRII CCWGG 1 cut(s) 215
EcoT14I CCWWGG 3 cut(s) 572, 1135, 1243
EcoT38I GRGCYC 2 cut(s) 215, 572
ErhI CCWWGG 3 cut(s) 572, 1135, 1243
FaqI GGGAC 2 cut(s) 233, 383
FauI CCCGC 1 cut(s) 1133
FauNDI CATATG 2 cut(s) 87, 1100
FokI GGATG 5 cut(s) 416, 458, 504, 613, 962
FriOI GRGCYC 2 cut(s) 215, 572
GsaI CCCAGC 1 cut(s) 713
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 3 cut(s) 570, 695, 1241
HapII CCGG 4 cut(s) 173, 271, 441, 460
HgaI GACGC 2 cut(s) 786, 1200
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HinfI GANTC 4 cut(s) 55, 401, 623, 874
HpaII CCGG 4 cut(s) 173, 271, 441, 460
HphI GGTGA 2 cut(s) 869, 991
Hpy166II GTNNAC 3 cut(s) 391, 515, 1096
Hpy188I TCNGA 1 cut(s) 781
Hpy188III TCNNGA 6 cut(s) 38, 398, 412, 489, 554, 1185
Hpy8I GTNNAC 3 cut(s) 391, 515, 1096
Hpy99I CGWCG 2 cut(s) 329, 338
HpyAV CCTTC 2 cut(s) 153, 671
HpyCH4III ACNGT 5 cut(s) 27, 124, 325, 374, 1182
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 9 cut(s) 195, 262, 361, 683, 903, 910, 1127, 1151, 1155
HpyF10VI GCNNNNNNNGC 4 cut(s) 545, 548, 692, 1238
HpyF3I CTNAG 5 cut(s) 183, 422, 543, 631, 1068
HpySE526I ACGT 1 cut(s) 407
Kzo9I GATC 2 cut(s) 588, 969
LmnI GCTCC 2 cut(s) 218, 1199
LweI GCATC 1 cut(s) 1012
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 6 cut(s) 62, 124, 425, 866, 925, 1182
MalI GATC 2 cut(s) 590, 971
MboI GATC 2 cut(s) 588, 969
MboII GAAGA 6 cut(s) 269, 340, 341, 655, 658, 925
MfeI CAATTG 1 cut(s) 1227
MhlI GDGCHC 2 cut(s) 215, 572
MluCI AATT 6 cut(s) 464, 804, 1073, 1227, 1250, 1259
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 1 cut(s) 179
MseI TTAA 2 cut(s) 752, 882
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 4 cut(s) 173, 271, 441, 460
MspR9I CCNGG 4 cut(s) 173, 217, 442, 460
MunI CAATTG 1 cut(s) 1227
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 545, 548, 692, 1238
NciI CCSGG 3 cut(s) 173, 442, 460
NcoI CCATGG 2 cut(s) 1135, 1243
NdeI CATATG 2 cut(s) 87, 1100
NdeII GATC 2 cut(s) 588, 969
NlaIV GGNNCC 3 cut(s) 371, 570, 1033
NmuCI GTSAC 4 cut(s) 425, 866, 925, 1182
NspI RCATGY 2 cut(s) 106, 1155
PaeI GCATGC 1 cut(s) 1155
PciI ACATGT 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PfeI GAWTC 3 cut(s) 55, 401, 874
PleI GAGTC 1 cut(s) 631
PpsI GAGTC 1 cut(s) 631
PscI ACATGT 1 cut(s) 102
PshAI GACNNNNGTC 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 215
Psp1406I AACGTT 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 709
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 3 cut(s) 371, 570, 1033
PspOMI GGGCCC 1 cut(s) 568
PspPI GGNCC 6 cut(s) 370, 388, 568, 569, 694, 1240
PstI CTGCAG 1 cut(s) 1129
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 752, 882
Sau3AI GATC 2 cut(s) 588, 969
Sau96I GGNCC 6 cut(s) 370, 388, 568, 569, 694, 1240
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 4 cut(s) 173, 217, 442, 460
SduI GDGCHC 2 cut(s) 215, 572
SfaNI GCATC 1 cut(s) 1012
SfcI CTRYAG 4 cut(s) 672, 840, 856, 1125
SinI GGWCC 2 cut(s) 370, 388
SmlI CTYRAG 3 cut(s) 508, 552, 881
SmoI CTYRAG 3 cut(s) 508, 552, 881
SphI GCATGC 1 cut(s) 1155
Sse9I AATT 6 cut(s) 464, 804, 1073, 1227, 1250, 1259
SsiI CCGC 6 cut(s) 176, 295, 548, 1044, 1140, 1238
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 4 cut(s) 171, 215, 440, 458
StyI CCWWGG 3 cut(s) 572, 1135, 1243
TaaI ACNGT 5 cut(s) 27, 124, 325, 374, 1182
TaiI ACGT 1 cut(s) 410
TaqI TCGA 6 cut(s) 37, 74, 231, 280, 327, 972
TasI AATT 6 cut(s) 464, 804, 1073, 1227, 1250, 1259
TatI WGTACW 1 cut(s) 119
TauI GCSGC 3 cut(s) 179, 551, 1241
TfiI GAWTC 3 cut(s) 55, 401, 874
Tru1I TTAA 2 cut(s) 752, 882
Tru9I TTAA 2 cut(s) 752, 882
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 4 cut(s) 425, 866, 925, 1182
Tsp45I GTSAC 4 cut(s) 425, 866, 925, 1182
TspDTI ATGAA 7 cut(s) 33, 315, 774, 798, 841, 1135, 1272
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 881
VpaK11BI GGWCC 2 cut(s) 370, 388
XapI RAATTY 3 cut(s) 464, 1073, 1259
XceI RCATGY 2 cut(s) 106, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.