MD15G1302100.v1.1

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001812
Physical Location & Seq
Reverse (-)
29065299 .. 29069559
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1302100.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGAGGCATTGGGACCAGTGGGTCGAAGAGGCTCTTCACAAGCTTGATCGTCTGCAGCTTCTTCGTTCTCTCAGACCCATTTACCTCCATAATGGACCAACCCAAGAAGCCCAGAAATCTGCCGAAGATGAATTCAATGTGTTCGATGAAATGCAGACATGGGACCGCTCGTCTGTGGAGGTTCACATTCCCGAACCCACATTCGAAAAATGGCTCCATGACATTCCCAGTTCCGGAGATGAGGAAGGTACCCAGAAGTTTAAAAAGCTGCTACTTTTCTCTGGAAATGACTATTTAGGCTTGAGTTCACATCCTACTATTGGAAAAGCTGCTGCTAAGGCAGCGCTAGAACACGGAATGGGCCCGAGGGGTTCTGCTTTAATTTGTGGATACACTGATTACCATAGGCGATTGGAGTCATGCTTAGCAGACTTAAAGAAGAAAGAGGATTGCCTTCTTTGTCCCACAGGGTTTGCAGCCAATATGGCCTTGATGGTAGCTTTGGGAAATGTTGGCTCTCTTTTGTCCGCTGGAAAAATGCCTTTAACTAATGAAAAGATTGCAGTTTTTTCTGATGCACTGAACCATGCGTCAATAATCGATGGTATTCGTCTTGCTGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGGCACACCTTAATGCGTTGTTATCAAGTTGCACAATGAGGAAGAAAGTCGTTGTGACTGATACCTTGTTTAGTATGGACGGAGACTTTGCACCAATCACTGAGCTTGTGAAGCTACGCAGGGAGCATGACTTTCTGTTAGTTATTGATGATGCTCATGGAACATTTGTTTGTGGAAAAAATGGTGGTGGAGTGGCTGAGGAATACAATTGTGAAGGCGATGTTGACATATGCGTCGGGACTTTGAGTAAGGCTGCAGGTTGCCATGGCGGATTCATCGCATGCAGCAAAAGGTGGAAGCAATTCATACAATCAAGGGGTCGCTCTTTTATATTTTCAACTGCTACGCCAGTACCAATTGCTGCTGCTGCTCATGCTGCTGTTTTTGTGGCAAGAAAGGAAACATGGCGTAGAAGGGAAATTTGGAATCGGGTGAAAGACTTTCGTGCTCTCACTGGAATTCCCATCAACAGTCCCATAATATCGCTCATAGTAGGGAGTGAAGAGAAGGCTCTGCAAGCCAGCCAGAGTTTGCTAAAATCTGGCTTCCATGTGACTGCAATCAGACCCCCAACAGTGCCACCCAACTCATGCAGGTTACGTGTGACATTGAGCGCAACGCACACGAGGAACGATGTCGAGAGGTTCACAGCTGCACTCTCCCGTTGCGTCAATTTCCGAGAAATTGGCATCCATGGCTCAAATGGATATGCAAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

462

Amino Acids

51.1

Weight (kDa)

8.36

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 89 - 441 8.4e-49 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 896
Acc36I ACCTGC 2 cut(s) 911, 1248
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 3 cut(s) 166, 528, 933
AcsI RAATTY 3 cut(s) 131, 1083, 1122
AfaI GTAC 2 cut(s) 250, 1017
AfeI AGCGCT 1 cut(s) 345
AfiI CCNNNNNNNGG 2 cut(s) 233, 320
AflIII ACRYGT 1 cut(s) 1264
AgsI TTSAA 2 cut(s) 136, 1002
AhdI GACNNNNNGTC 1 cut(s) 169
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AluBI AGCT 8 cut(s) 43, 58, 268, 329, 500, 769, 778, 1316
AluI AGCT 8 cut(s) 43, 58, 268, 329, 500, 769, 778, 1316
Alw21I GWGCWC 1 cut(s) 1114
Alw26I GTCTC 1 cut(s) 741
Ama87I CYCGRG 1 cut(s) 364
Aor13HI TCCGGA 1 cut(s) 233
Aor51HI AGCGCT 1 cut(s) 345
AoxI GGCC 2 cut(s) 361, 486
ApaI GGGCCC 1 cut(s) 365
ApoI RAATTY 3 cut(s) 131, 1083, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
Asp700I GAANNNNTTC 1 cut(s) 965
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 2 cut(s) 346, 1280
AspS9I GGNCC 5 cut(s) 13, 95, 163, 361, 362
AsuHPI GGTGA 1 cut(s) 1108
AsuII TTCGAA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 3 cut(s) 13, 95, 163
BaeGI GKGCMC 1 cut(s) 365
BaeI ACNNNNGTAYC 2 cut(s) 999, 1032
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 365
BauI CACGAG 1 cut(s) 1288
Bbv12I GWGCWC 1 cut(s) 1114
BbvCI CCTCAGC 1 cut(s) 861
BccI CCATC 3 cut(s) 487, 596, 1136
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciVI GTATCC 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 741
BfaI CTAG 1 cut(s) 347
BfmI CTRYAG 4 cut(s) 53, 633, 649, 918
BfoI RGCGCY 1 cut(s) 347
BfuAI ACCTGC 2 cut(s) 911, 1248
BfuI GTATCC 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 485
BlpI GCTNAGC 1 cut(s) 424
Bme18I GGWCC 3 cut(s) 13, 95, 163
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 364
BmgT120I GGNCC 5 cut(s) 13, 95, 163, 361, 362
BmiI GGNNCC 5 cut(s) 14, 164, 215, 250, 363
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 3 cut(s) 565, 805, 1362
BmuI ACTGGG 1 cut(s) 222
Bpu10I CCTNAGC 2 cut(s) 336, 861
Bpu1102I GCTNAGC 1 cut(s) 424
Bpu14I TTCGAA 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 322
Bsa29I ATCGAT 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 1265
BsaBI GATNNNNATC 1 cut(s) 644
BsaJI CCNNGG 3 cut(s) 365, 928, 1357
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 320
Bse1I ACTGG 4 cut(s) 16, 228, 1013, 1123
Bse8I GATNNNNATC 1 cut(s) 644
BseAI TCCGGA 1 cut(s) 233
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 365, 928, 1357
BseGI GGATG 2 cut(s) 310, 1353
BseJI GATNNNNATC 1 cut(s) 644
BseLI CCNNNNNNNGG 2 cut(s) 233, 320
BseMII CTCAG 3 cut(s) 85, 756, 852
BseNI ACTGG 4 cut(s) 16, 228, 1013, 1123
BseSI GKGCMC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 1302
BshFI GGCC 2 cut(s) 363, 488
BshNI GGYRCC 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 1114
BsiHKCI CYCGRG 1 cut(s) 364
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 5 cut(s) 26, 176, 447, 916, 1122
BslI CCNNNNNNNGG 2 cut(s) 233, 320
BsmAI GTCTC 1 cut(s) 741
BsmFI GGGAC 5 cut(s) 26, 176, 447, 916, 1122
BsnI GGCC 2 cut(s) 363, 488
BsoBI CYCGRG 1 cut(s) 364
Bsp119I TTCGAA 1 cut(s) 204
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 365, 1114
Bsp13I TCCGGA 1 cut(s) 233
Bsp143I GATC 1 cut(s) 46
Bsp1720I GCTNAGC 1 cut(s) 424
Bsp19I CCATGG 2 cut(s) 928, 1357
BspACI CCGC 3 cut(s) 166, 528, 933
BspANI GGCC 2 cut(s) 363, 488
BspCNI CTCAG 3 cut(s) 84, 757, 853
BspDI ATCGAT 1 cut(s) 600
BspEI TCCGGA 1 cut(s) 233
BspLI GGNNCC 5 cut(s) 14, 164, 215, 250, 363
BspMAI CTGCAG 2 cut(s) 57, 922
BspMI ACCTGC 2 cut(s) 911, 1248
BspQI GCTCTTC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 4 cut(s) 16, 228, 1013, 1123
BssECI CCNNGG 3 cut(s) 365, 928, 1357
BssMI GATC 1 cut(s) 46
BssSI CACGAG 1 cut(s) 1288
BssT1I CCWWGG 2 cut(s) 928, 1357
Bst2BI CACGAG 1 cut(s) 1288
Bst4CI ACNGT 2 cut(s) 1136, 1240
Bst6I CTCTTC 3 cut(s) 21, 39, 1161
BstBAI YACGTR 1 cut(s) 1265
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 946, 1182, 1186
BstDEI CTNAG 5 cut(s) 71, 336, 424, 765, 861
BstDSI CCRYGG 2 cut(s) 928, 1357
BstF5I GGATG 2 cut(s) 310, 1353
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 2 cut(s) 346, 1280
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 1 cut(s) 741
BstMBI GATC 1 cut(s) 46
BstMWI GCNNNNNNNGC 9 cut(s) 338, 341, 485, 775, 1031, 1037, 1040, 1181, 1359
BstNSI RCATGY 1 cut(s) 948
BstSFI CTRYAG 4 cut(s) 53, 633, 649, 918
BstSLI GKGCMC 1 cut(s) 365
Bsu15I ATCGAT 1 cut(s) 600
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 2 cut(s) 363, 488
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 2 cut(s) 928, 1357
BtgZI GCGATG 2 cut(s) 897, 925
BtsCI GGATG 2 cut(s) 310, 1353
BtsIMutI CAGTG 6 cut(s) 23, 393, 578, 762, 1116, 1245
BveI ACCTGC 2 cut(s) 911, 1248
Cac8I GCNNGC 3 cut(s) 946, 1182, 1186
CfoI GCGC 2 cut(s) 346, 1280
Cfr13I GGNCC 5 cut(s) 13, 95, 163, 361, 362
ClaI ATCGAT 1 cut(s) 600
CseI GACGC 3 cut(s) 579, 886, 1321
Csp6I GTAC 2 cut(s) 249, 1016
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviQI GTAC 2 cut(s) 249, 1016
DdeI CTNAG 5 cut(s) 71, 336, 424, 765, 861
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
DraI TTTAAA 1 cut(s) 262
DrdI GACNNNNNNGTC 2 cut(s) 20, 896
DriI GACNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 2 cut(s) 20, 896
Eam1104I CTCTTC 3 cut(s) 21, 39, 1161
Eam1105I GACNNNNNGTC 1 cut(s) 169
EarI CTCTTC 3 cut(s) 21, 39, 1161
EciI GGCGGA 1 cut(s) 948
Eco130I CCWWGG 2 cut(s) 928, 1357
Eco24I GRGCYC 1 cut(s) 365
Eco47I GGWCC 3 cut(s) 13, 95, 163
Eco47III AGCGCT 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 364
EcoRI GAATTC 2 cut(s) 131, 1122
EcoT14I CCWWGG 2 cut(s) 928, 1357
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 2 cut(s) 928, 1357
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 5 cut(s) 26, 176, 447, 916, 1122
FauNDI CATATG 1 cut(s) 893
FokI GGATG 2 cut(s) 297, 1340
FriOI GRGCYC 1 cut(s) 365
FspBI CTAG 1 cut(s) 347
GlaI GCGC 2 cut(s) 345, 1279
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 2 cut(s) 363, 488
HapII CCGG 1 cut(s) 234
HgaI GACGC 3 cut(s) 579, 886, 1321
HhaI GCGC 2 cut(s) 346, 1280
Hin6I GCGC 2 cut(s) 344, 1278
HinP1I GCGC 2 cut(s) 344, 1278
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 3 cut(s) 416, 936, 1090
HpaII CCGG 1 cut(s) 234
HphI GGTGA 1 cut(s) 1108
Hpy166II GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy188I TCNGA 4 cut(s) 74, 574, 1229, 1343
Hpy188III TCNNGA 5 cut(s) 191, 234, 282, 901, 1303
Hpy8I GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 5 cut(s) 239, 464, 872, 1071, 1165
HpyCH4III ACNGT 2 cut(s) 1136, 1240
HpyCH4IV ACGT 1 cut(s) 1264
HpyF10VI GCNNNNNNNGC 9 cut(s) 338, 341, 485, 775, 1031, 1037, 1040, 1181, 1359
HpyF3I CTNAG 5 cut(s) 71, 336, 424, 765, 861
HpySE526I ACGT 1 cut(s) 1264
HspAI GCGC 2 cut(s) 344, 1278
Kpn2I TCCGGA 1 cut(s) 233
KpnI GGTACC 1 cut(s) 252
Kzo9I GATC 1 cut(s) 46
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 219, 787
LweI GCATC 3 cut(s) 565, 805, 1362
MaeI CTAG 1 cut(s) 347
MaeII ACGT 1 cut(s) 1264
MaeIII GTNAC 5 cut(s) 659, 718, 1216, 1260, 1267
MalI GATC 1 cut(s) 48
MbiI CCGCTC 1 cut(s) 168
MboI GATC 1 cut(s) 46
MboII GAAGA 7 cut(s) 26, 38, 53, 137, 451, 718, 1178
MfeI CAATTG 2 cut(s) 871, 1020
MhlI GDGCHC 2 cut(s) 365, 1114
MluCI AATT 9 cut(s) 131, 381, 871, 965, 1020, 1083, 1122, 1336, 1347
MlyI GAGTC 1 cut(s) 425
MroI TCCGGA 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 5 cut(s) 261, 380, 434, 545, 675
MslI CAYNNNNRTG 1 cut(s) 675
MspA1I CMGCKG 2 cut(s) 530, 1316
MspI CCGG 1 cut(s) 234
MunI CAATTG 2 cut(s) 871, 1020
MwoI GCNNNNNNNGC 9 cut(s) 338, 341, 485, 775, 1031, 1037, 1040, 1181, 1359
NcoI CCATGG 2 cut(s) 928, 1357
NdeI CATATG 1 cut(s) 893
NdeII GATC 1 cut(s) 46
NlaIV GGNNCC 5 cut(s) 14, 164, 215, 250, 363
NmuCI GTSAC 4 cut(s) 659, 718, 1216, 1267
NspI RCATGY 1 cut(s) 948
NspV TTCGAA 1 cut(s) 204
PaeI GCATGC 1 cut(s) 948
PciSI GCTCTTC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 2 cut(s) 936, 1090
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Ppu21I YACGTR 1 cut(s) 1265
PspN4I GGNNCC 5 cut(s) 14, 164, 215, 250, 363
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 5 cut(s) 13, 95, 163, 361, 362
PstI CTGCAG 2 cut(s) 57, 922
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 2 cut(s) 250, 1017
RsaNI GTAC 2 cut(s) 249, 1016
RseI CAYNNNNRTG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 5 cut(s) 261, 380, 434, 545, 675
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 5 cut(s) 13, 95, 163, 361, 362
SchI GAGTC 1 cut(s) 425
SduI GDGCHC 2 cut(s) 365, 1114
SfaNI GCATC 3 cut(s) 565, 805, 1362
SfcI CTRYAG 4 cut(s) 53, 633, 649, 918
SfuI TTCGAA 1 cut(s) 204
SinI GGWCC 3 cut(s) 13, 95, 163
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SphI GCATGC 1 cut(s) 948
Sse9I AATT 9 cut(s) 131, 381, 871, 965, 1020, 1083, 1122, 1336, 1347
SsiI CCGC 3 cut(s) 166, 528, 933
SspMI CTAG 1 cut(s) 347
StyI CCWWGG 2 cut(s) 928, 1357
TaaI ACNGT 2 cut(s) 1136, 1240
TaiI ACGT 1 cut(s) 1267
TaqI TCGA 5 cut(s) 24, 144, 204, 600, 1302
TasI AATT 9 cut(s) 131, 381, 871, 965, 1020, 1083, 1122, 1336, 1347
TfiI GAWTC 2 cut(s) 936, 1090
Tru1I TTAA 5 cut(s) 261, 380, 434, 545, 675
Tru9I TTAA 5 cut(s) 261, 380, 434, 545, 675
TscAI CASTG 6 cut(s) 23, 400, 585, 769, 1123, 1245
TseFI GTSAC 4 cut(s) 659, 718, 1216, 1267
Tsp45I GTSAC 4 cut(s) 659, 718, 1216, 1267
TspDTI ATGAA 6 cut(s) 144, 162, 567, 634, 928, 958
TspGWI ACGGA 2 cut(s) 369, 759
TspRI CASTG 6 cut(s) 23, 400, 585, 769, 1123, 1245
VpaK11BI GGWCC 3 cut(s) 13, 95, 163
XapI RAATTY 3 cut(s) 131, 1083, 1122
XceI RCATGY 1 cut(s) 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 1364
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.