Rh6BG010800

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001812
Physical Location & Seq
Reverse (-)
1631877 .. 1634970
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG010800.1

Sequence Viewer

Length: 801 bp
ATGGGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACTTCCGCAATGAACCTCAAAAACCCACCACCACCATCGAAGATGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCTGGAGATGAGGAAGATACCCGGAGATTCAGAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGCTCTGCTTTGATCTGTGGATATACAGATTACCATAGGCGACTGGAGTCATCCTTAGCGGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCTTGATGAACAAGAAAGAAAGAAAGAAGGTGAGGGAGGTGGGGATCTTGAAGAAGGATCCTGAGACCCTGAAGGAGCAGATTGATAAGTTGAATAAGCAGAGAGATGAAATGACTCGAATTTTCTGGAAATCTATAAAAGATAAGCTTATTTTGCCCTTTCTGGAACTGGATATCAAGTACTTTGATCTTGGCCTCCCTAATCGTGATGCCACCAGTGATAGAGTCACAATTGAAAGTGCAGAAGCTACTCTTAAATTTTTGAGCTGCAATATCCGTTGCAAACAAATTGCAGCAGTGACAACAAGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

266

Amino Acids

30.56

Weight (kDa)

7.64

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Wbp11 PF09429 166 - 197 3.8e-06 WW domain binding protein 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 137
AciI CCGC 3 cut(s) 88, 341, 428
AclI AACGTT 1 cut(s) 200
AclWI GGATC 3 cut(s) 539, 539, 552
AcsI RAATTY 2 cut(s) 606, 743
AcuI CTGAAG 1 cut(s) 578
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 2 cut(s) 233, 320
AgsI TTSAA 5 cut(s) 47, 436, 538, 580, 722
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 5 cut(s) 157, 268, 634, 734, 753
AluI AGCT 5 cut(s) 157, 268, 634, 734, 753
Alw26I GTCTC 2 cut(s) 72, 545
AlwI GGATC 3 cut(s) 539, 539, 552
AoxI GGCC 3 cut(s) 361, 486, 679
ApaI GGGCCC 1 cut(s) 365
ApeKI GCWGC 6 cut(s) 154, 329, 332, 476, 753, 779
ApoI RAATTY 2 cut(s) 606, 743
AspS9I GGNCC 4 cut(s) 163, 181, 361, 362
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 529
AvaII GGWCC 2 cut(s) 163, 181
BaeGI GKGCMC 1 cut(s) 365
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 3 cut(s) 8, 365, 374
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 6 cut(s) 166, 316, 319, 488, 740, 791
BccI CCATC 2 cut(s) 86, 125
BciT130I CCWGG 1 cut(s) 10
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 72, 545
BfmI CTRYAG 1 cut(s) 465
BglI GCCNNNNNGGC 2 cut(s) 369, 485
BisI GCNGC 7 cut(s) 155, 330, 333, 342, 477, 754, 780
BlsI GCNGC 7 cut(s) 156, 331, 334, 343, 478, 755, 781
BmcAI AGTACT 1 cut(s) 668
Bme1390I CCNGG 2 cut(s) 10, 253
Bme18I GGWCC 2 cut(s) 163, 181
BmgT120I GGNCC 4 cut(s) 163, 181, 361, 362
BmiI GGNNCC 4 cut(s) 7, 164, 363, 546
BmrFI CCNGG 2 cut(s) 10, 253
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 1 cut(s) 685
BmuI ACTGGG 2 cut(s) 28, 222
BoxI GACNNNNGTC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 2 cut(s) 255, 434
Bpu10I CCTNAGC 2 cut(s) 336, 424
BpuEI CTTGAG 2 cut(s) 322, 330
BpuMI CCSGG 1 cut(s) 253
BsaI GGTCTC 2 cut(s) 72, 545
BsaJI CCNNGG 3 cut(s) 9, 174, 365
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 320
Bse1I ACTGG 5 cut(s) 23, 228, 417, 660, 702
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 3 cut(s) 9, 174, 365
BseGI GGATG 3 cut(s) 222, 310, 419
BseLI CCNNNNNNNGG 2 cut(s) 233, 320
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 229, 540
BseNI ACTGG 5 cut(s) 23, 228, 417, 660, 702
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 6 cut(s) 166, 316, 319, 488, 740, 791
BsgI GTGCAG 1 cut(s) 747
BshFI GGCC 3 cut(s) 363, 488, 681
BsiSI CCGG 2 cut(s) 64, 253
BslFI GGGAC 2 cut(s) 26, 176
BslI CCNNNNNNNGG 2 cut(s) 233, 320
BsmAI GTCTC 2 cut(s) 72, 545
BsmFI GGGAC 2 cut(s) 26, 176
BsnI GGCC 3 cut(s) 363, 488, 681
Bso31I GGTCTC 2 cut(s) 72, 545
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 8, 365, 374
Bsp143I GATC 4 cut(s) 381, 531, 544, 673
BspACI CCGC 3 cut(s) 88, 341, 428
BspANI GGCC 3 cut(s) 363, 488, 681
BspCNI CTCAG 2 cut(s) 228, 541
BspLI GGNNCC 4 cut(s) 7, 164, 363, 546
BspPI GGATC 3 cut(s) 539, 539, 552
BspTNI GGTCTC 2 cut(s) 72, 545
BsrDI GCAATG 1 cut(s) 97
BsrI ACTGG 5 cut(s) 23, 228, 417, 660, 702
BssECI CCNNGG 3 cut(s) 9, 174, 365
BssMI GATC 4 cut(s) 381, 531, 544, 673
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 4 cut(s) 215, 336, 424, 549
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 3 cut(s) 222, 310, 419
BstKTI GATC 4 cut(s) 384, 534, 547, 676
BstMAI GTCTC 2 cut(s) 72, 545
BstMBI GATC 4 cut(s) 381, 531, 544, 673
BstMWI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
BstNI CCWGG 1 cut(s) 10
BstPAI GACNNNNGTC 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 8, 251
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 6 cut(s) 166, 316, 319, 488, 740, 791
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 2 cut(s) 531, 544
BstYI RGATCY 2 cut(s) 531, 544
BsuRI GGCC 3 cut(s) 363, 488, 681
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 3 cut(s) 222, 310, 419
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 3 cut(s) 223, 709, 789
Cac8I GCNNGC 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 163, 181, 361, 362
Csp6I GTAC 1 cut(s) 667
CviAII CATG 1 cut(s) 353
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 4 cut(s) 215, 336, 424, 549
DpnI GATC 4 cut(s) 383, 533, 546, 675
DpnII GATC 4 cut(s) 381, 531, 544, 673
DrdI GACNNNNNNGTC 2 cut(s) 20, 137
DseDI GACNNNNNNGTC 2 cut(s) 20, 137
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 3 cut(s) 8, 365, 374
Eco31I GGTCTC 2 cut(s) 72, 545
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 163, 181
Eco57I CTGAAG 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 8
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 3 cut(s) 8, 365, 374
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 1 cut(s) 356
FaiI YATR 5 cut(s) 354, 393, 405, 485, 623
FalI AAGNNNNNCTT 4 cut(s) 618, 650, 723, 755
FaqI GGGAC 2 cut(s) 26, 176
FatI CATG 1 cut(s) 352
Fnu4HI GCNGC 7 cut(s) 155, 330, 333, 342, 477, 754, 780
FokI GGATG 3 cut(s) 209, 297, 406
FriOI GRGCYC 3 cut(s) 8, 365, 374
Fsp4HI GCNGC 7 cut(s) 155, 330, 333, 342, 477, 754, 780
GluI GCNGC 7 cut(s) 155, 330, 333, 342, 477, 754, 780
GsuI CTGGAG 2 cut(s) 255, 434
HaeIII GGCC 3 cut(s) 363, 488, 681
HapII CCGG 2 cut(s) 64, 253
Hin1II CATG 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 194, 258, 416, 601, 711
HpaII CCGG 2 cut(s) 64, 253
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 2 cut(s) 184, 308
Hpy188I TCNGA 2 cut(s) 74, 263
Hpy8I GTNNAC 2 cut(s) 184, 308
HpyAV CCTTC 4 cut(s) 464, 508, 535, 553
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 7 cut(s) 55, 154, 476, 728, 756, 768, 779
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
HpyF3I CTNAG 4 cut(s) 215, 336, 424, 549
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 1 cut(s) 356
Kzo9I GATC 4 cut(s) 381, 531, 544, 673
LmnI GCTCC 2 cut(s) 11, 562
Lsp1109I GCAGC 6 cut(s) 166, 316, 319, 488, 740, 791
LweI GCATC 1 cut(s) 685
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 218, 712, 784
MalI GATC 4 cut(s) 383, 533, 546, 675
MboI GATC 4 cut(s) 381, 531, 544, 673
MboII GAAGA 6 cut(s) 133, 134, 257, 448, 451, 550
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 531, 544
MhlI GDGCHC 3 cut(s) 8, 365, 374
MluCI AATT 4 cut(s) 606, 717, 743, 774
MlyI GAGTC 3 cut(s) 425, 595, 720
MmeI TCCRAC 1 cut(s) 97
MseI TTAA 2 cut(s) 741, 799
MspI CCGG 2 cut(s) 64, 253
MspR9I CCNGG 2 cut(s) 10, 253
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 5 cut(s) 338, 341, 369, 485, 640
NciI CCSGG 1 cut(s) 253
NdeII GATC 4 cut(s) 381, 531, 544, 673
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 4 cut(s) 7, 164, 363, 546
NmuCI GTSAC 3 cut(s) 218, 712, 784
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 194, 258
PkrI GCNGC 7 cut(s) 156, 331, 334, 343, 478, 755, 781
PleI GAGTC 3 cut(s) 424, 595, 719
PpsI GAGTC 3 cut(s) 424, 595, 719
PshAI GACNNNNGTC 1 cut(s) 415
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 7, 164, 363, 546
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 4 cut(s) 163, 181, 361, 362
PsuI RGATCY 2 cut(s) 531, 544
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
SaqAI TTAA 2 cut(s) 741, 799
SatI GCNGC 7 cut(s) 155, 330, 333, 342, 477, 754, 780
Sau3AI GATC 4 cut(s) 381, 531, 544, 673
Sau96I GGNCC 4 cut(s) 163, 181, 361, 362
ScaI AGTACT 1 cut(s) 668
SchI GAGTC 3 cut(s) 425, 595, 720
ScrFI CCNGG 2 cut(s) 10, 253
SduI GDGCHC 3 cut(s) 8, 365, 374
SfaNI GCATC 1 cut(s) 685
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 2 cut(s) 163, 181
SmlI CTYRAG 2 cut(s) 301, 345
SmoI CTYRAG 2 cut(s) 301, 345
Sse9I AATT 4 cut(s) 606, 717, 743, 774
SsiI CCGC 3 cut(s) 88, 341, 428
StyD4I CCNGG 2 cut(s) 8, 251
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 203
TaqI TCGA 3 cut(s) 24, 120, 604
TasI AATT 4 cut(s) 606, 717, 743, 774
TatI WGTACW 1 cut(s) 666
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 194, 258
Tru1I TTAA 2 cut(s) 741, 799
Tru9I TTAA 2 cut(s) 741, 799
TscAI CASTG 3 cut(s) 223, 709, 789
TseFI GTSAC 3 cut(s) 218, 712, 784
TseI GCWGC 6 cut(s) 154, 329, 332, 476, 753, 779
Tsp45I GTSAC 3 cut(s) 218, 712, 784
TspDTI ATGAA 3 cut(s) 108, 509, 609
TspGWI ACGGA 2 cut(s) 163, 752
TspRI CASTG 3 cut(s) 223, 709, 789
VpaK11BI GGWCC 2 cut(s) 163, 181
XapI RAATTY 2 cut(s) 606, 743
ZrmI AGTACT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.