Rw1G018340

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001812
Physical Location & Seq
Forward (+)
40403112 .. 40406904
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018340.1

Sequence Viewer

Length: 1386 bp
ATGAGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACCTCCGCAATGAACCTCAAAAACCCACCACCACCGTCGAAGACGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCCGGGGATGAGGAAGATACCCGGAGATTCAGAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGCTCTACTTTGATCTGTGGATATACGGATTACCATAGGCGACTGGAGTCATCCTTAGCGGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCTTGATGGTAGTGTTGGGAAATGTTGGCTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATTGCCATTTTTTCTGATGAACTGAACCATGCGTCGATAATTGATGGTATTCGTCTTGCAGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGATTCACCTTAAGGCATTGTTATCCAGTTGCACAATGCAGAAGAAAGTTGTTGTGACTGATGGCTTGTTTAGTGTGGATGGAGACTTCGCACCAATGATCGAGCTGGTGAAACTACGCAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTTGTTTGCGGAAAAAATGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAGAGATGTTGACATATGTGTTGGCACTTTGAGTAAGGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCGTTTATATTTTCAACTGCTACACCATTACCAATTGCTGCTGCGGCCCATGCTGCTATTATTGTCGCAAGAAAGGAAACATGGCGTAGAAGGGCAATTTGGAGTCGGGTGCAAGATTTTCGTGCTCTCACTGGAATTTCCATAAACAGTCCAATAATATCGCTTGTCGTAGGAAGCGAAGAGAAGGCTTTGAAAGCGAGCCGGAGTTTGTTGAAATTGGGTTTCCATGGGACTGCAATCAGACCCCCAACGGTGCCGCCCAACTCATGCAGGCTACGAGTGACTTTGAGTGCAACACACACAAGGGCTGATATTGAGAGGTTCACAACTGCACTCTCCAATTGTGTAAATTTCCAAGAAATTGGCATCCCTGGCTCCAATGGATACGCAAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

461

Amino Acids

51.06

Weight (kDa)

8.66

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 89 - 441 2e-44 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 137
Acc36I ACCTGC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 1240
AciI CCGC 7 cut(s) 88, 341, 428, 837, 933, 1031, 1244
AclI AACGTT 1 cut(s) 200
AcsI RAATTY 3 cut(s) 866, 1122, 1336
AfiI CCNNNNNNNGG 3 cut(s) 233, 234, 320
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 5 cut(s) 47, 436, 1002, 1180, 1201
AjnI CCWGG 2 cut(s) 8, 1357
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AluBI AGCT 4 cut(s) 6, 157, 268, 769
AluI AGCT 4 cut(s) 6, 157, 268, 769
Alw21I GWGCWC 2 cut(s) 8, 1114
Alw26I GTCTC 2 cut(s) 72, 741
AoxI GGCC 3 cut(s) 361, 486, 1032
ApaI GGGCCC 1 cut(s) 365
ApoI RAATTY 3 cut(s) 866, 1122, 1336
AspS9I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
AsuC2I CCSGG 2 cut(s) 235, 253
AsuHPI GGTGA 2 cut(s) 662, 784
AvaII GGWCC 2 cut(s) 163, 181
BaeGI GKGCMC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 1240
BanII GRGCYC 3 cut(s) 8, 365, 374
BauI CACGAG 1 cut(s) 978
BbsI GAAGAC 2 cut(s) 129, 133
Bbv12I GWGCWC 2 cut(s) 8, 1114
BbvCI CCTCAGC 1 cut(s) 861
BccI CCATC 6 cut(s) 86, 487, 596, 719, 737, 951
BcgI CGANNNNNNTGC 2 cut(s) 1088, 1122
BciT130I CCWGG 2 cut(s) 10, 1359
BciVI GTATCC 1 cut(s) 1364
BcnI CCSGG 2 cut(s) 235, 253
BcoDI GTCTC 2 cut(s) 72, 741
BfmI CTRYAG 4 cut(s) 465, 633, 649, 918
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 911
BfuI GTATCC 1 cut(s) 1364
BglI GCCNNNNNGGC 2 cut(s) 369, 485
Bme1390I CCNGG 4 cut(s) 10, 235, 253, 1359
Bme18I GGWCC 2 cut(s) 163, 181
BmgT120I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
BmiI GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
BmrFI CCNGG 4 cut(s) 10, 235, 253, 1359
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 2 cut(s) 805, 1362
BmuI ACTGGG 2 cut(s) 28, 222
BoxI GACNNNNGTC 1 cut(s) 415
BpiI GAAGAC 2 cut(s) 129, 133
BpmI CTGGAG 1 cut(s) 434
Bpu10I CCTNAGC 3 cut(s) 336, 424, 861
BpuEI CTTGAG 2 cut(s) 322, 330
BpuMI CCSGG 2 cut(s) 235, 253
BsaBI GATNNNNATC 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 7 cut(s) 9, 174, 234, 365, 928, 1213, 1357
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 234, 320
Bse1I ACTGG 5 cut(s) 23, 228, 417, 690, 1123
Bse3DI GCAATG 2 cut(s) 97, 939
Bse8I GATNNNNATC 1 cut(s) 644
BseBI CCWGG 2 cut(s) 10, 1359
BseDI CCNNGG 7 cut(s) 9, 174, 234, 365, 928, 1213, 1357
BseGI GGATG 6 cut(s) 222, 244, 310, 419, 748, 1353
BseJI GATNNNNATC 1 cut(s) 644
BseLI CCNNNNNNNGG 3 cut(s) 233, 234, 320
BseMI GCAATG 2 cut(s) 97, 939
BseMII CTCAG 2 cut(s) 229, 852
BseNI ACTGG 5 cut(s) 23, 228, 417, 690, 1123
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 1302
BshFI GGCC 3 cut(s) 363, 488, 1034
BshNI GGYRCC 1 cut(s) 1240
BsiHKAI GWGCWC 2 cut(s) 8, 1114
BsiSI CCGG 4 cut(s) 64, 234, 253, 1189
BslFI GGGAC 3 cut(s) 26, 176, 1231
BslI CCNNNNNNNGG 3 cut(s) 233, 234, 320
BsmAI GTCTC 2 cut(s) 72, 741
BsmFI GGGAC 3 cut(s) 26, 176, 1231
BsnI GGCC 3 cut(s) 363, 488, 1034
Bso31I GGTCTC 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 4 cut(s) 8, 365, 374, 1114
Bsp143I GATC 2 cut(s) 381, 762
Bsp19I CCATGG 2 cut(s) 928, 1213
BspACI CCGC 7 cut(s) 88, 341, 428, 837, 933, 1031, 1244
BspANI GGCC 3 cut(s) 363, 488, 1034
BspCNI CTCAG 2 cut(s) 228, 853
BspLI GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 1 cut(s) 911
BspT107I GGYRCC 1 cut(s) 1240
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 97, 939
BsrI ACTGG 5 cut(s) 23, 228, 417, 690, 1123
BssECI CCNNGG 7 cut(s) 9, 174, 234, 365, 928, 1213, 1357
BssMI GATC 2 cut(s) 381, 762
BssSI CACGAG 1 cut(s) 978
BssT1I CCWWGG 3 cut(s) 365, 928, 1213
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 2 cut(s) 10, 1359
Bst4CI ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
Bst6I CTCTTC 1 cut(s) 1161
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 5 cut(s) 53, 785, 946, 1186, 1259
BstDEI CTNAG 4 cut(s) 215, 336, 424, 861
BstDSI CCRYGG 3 cut(s) 174, 928, 1213
BstF5I GGATG 6 cut(s) 222, 244, 310, 419, 748, 1353
BstKTI GATC 2 cut(s) 384, 765
BstMAI GTCTC 2 cut(s) 72, 741
BstMBI GATC 2 cut(s) 381, 762
BstMWI GCNNNNNNNGC 9 cut(s) 338, 341, 369, 485, 1031, 1037, 1040, 1181, 1359
BstNI CCWGG 2 cut(s) 10, 1359
BstNSI RCATGY 1 cut(s) 948
BstPAI GACNNNNGTC 1 cut(s) 415
BstSCI CCNGG 4 cut(s) 8, 233, 251, 1357
BstSFI CTRYAG 4 cut(s) 465, 633, 649, 918
BstSLI GKGCMC 1 cut(s) 365
BstV2I GAAGAC 2 cut(s) 129, 133
BstXI CCANNNNNNTGG 1 cut(s) 1349
BsuI GTATCC 1 cut(s) 1364
BsuRI GGCC 3 cut(s) 363, 488, 1034
BtgI CCRYGG 3 cut(s) 174, 928, 1213
BtsCI GGATG 6 cut(s) 222, 244, 310, 419, 748, 1353
BtsIMutI CAGTG 2 cut(s) 223, 1116
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 5 cut(s) 53, 785, 946, 1186, 1259
Cfr13I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
CseI GACGC 2 cut(s) 579, 993
DdeI CTNAG 4 cut(s) 215, 336, 424, 861
DpnI GATC 2 cut(s) 383, 764
DpnII GATC 2 cut(s) 381, 762
DrdI GACNNNNNNGTC 2 cut(s) 20, 137
DseDI GACNNNNNNGTC 2 cut(s) 20, 137
Eam1104I CTCTTC 1 cut(s) 1161
EarI CTCTTC 1 cut(s) 1161
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 365, 928, 1213
Eco24I GRGCYC 3 cut(s) 8, 365, 374
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 163, 181
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 8, 1357
EcoT14I CCWWGG 3 cut(s) 365, 928, 1213
EcoT38I GRGCYC 3 cut(s) 8, 365, 374
ErhI CCWWGG 3 cut(s) 365, 928, 1213
FaqI GGGAC 3 cut(s) 26, 176, 1231
FauI CCCGC 1 cut(s) 926
FauNDI CATATG 1 cut(s) 893
FokI GGATG 6 cut(s) 209, 251, 297, 406, 755, 1340
FriOI GRGCYC 3 cut(s) 8, 365, 374
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 3 cut(s) 363, 488, 1034
HapII CCGG 4 cut(s) 64, 234, 253, 1189
HgaI GACGC 2 cut(s) 579, 993
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HinfI GANTC 5 cut(s) 194, 258, 416, 667, 1090
HpaII CCGG 4 cut(s) 64, 234, 253, 1189
HphI GGTGA 2 cut(s) 662, 784
Hpy166II GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy188I TCNGA 4 cut(s) 74, 263, 574, 1229
Hpy188III TCNNGA 5 cut(s) 191, 205, 282, 347, 978
Hpy8I GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy99I CGWCG 3 cut(s) 122, 131, 595
HpyAV CCTTC 3 cut(s) 464, 1071, 1165
HpyCH4III ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
HpyCH4IV ACGT 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 9 cut(s) 338, 341, 369, 485, 1031, 1037, 1040, 1181, 1359
HpyF3I CTNAG 4 cut(s) 215, 336, 424, 861
HpySE526I ACGT 1 cut(s) 200
Kzo9I GATC 2 cut(s) 381, 762
LmnI GCTCC 2 cut(s) 11, 1367
LweI GCATC 2 cut(s) 805, 1362
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 5 cut(s) 218, 659, 718, 975, 1267
MalI GATC 2 cut(s) 383, 764
MboI GATC 2 cut(s) 381, 762
MboII GAAGA 7 cut(s) 133, 134, 257, 448, 451, 718, 1178
MfeI CAATTG 2 cut(s) 1020, 1327
MhlI GDGCHC 4 cut(s) 8, 365, 374, 1114
MluCI AATT 9 cut(s) 597, 866, 1020, 1083, 1122, 1202, 1327, 1336, 1347
MlyI GAGTC 2 cut(s) 425, 1099
MmeI TCCRAC 1 cut(s) 97
MseI TTAA 2 cut(s) 545, 675
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 4 cut(s) 64, 234, 253, 1189
MspR9I CCNGG 4 cut(s) 10, 235, 253, 1359
MunI CAATTG 2 cut(s) 1020, 1327
MvaI CCWGG 2 cut(s) 10, 1359
MwoI GCNNNNNNNGC 9 cut(s) 338, 341, 369, 485, 1031, 1037, 1040, 1181, 1359
NciI CCSGG 2 cut(s) 235, 253
NcoI CCATGG 2 cut(s) 928, 1213
NdeI CATATG 1 cut(s) 893
NdeII GATC 2 cut(s) 381, 762
NlaIV GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
NmuCI GTSAC 5 cut(s) 218, 659, 718, 975, 1267
NspI RCATGY 1 cut(s) 948
PaeI GCATGC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 1161
PfeI GAWTC 3 cut(s) 194, 258, 667
PleI GAGTC 2 cut(s) 424, 1098
PpsI GAGTC 2 cut(s) 424, 1098
PshAI GACNNNNGTC 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 8
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 8, 1357
PspGI CCWGG 2 cut(s) 8, 1357
PspN4I GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 5 cut(s) 163, 181, 361, 362, 1033
PstI CTGCAG 1 cut(s) 922
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 545, 675
Sau3AI GATC 2 cut(s) 381, 762
Sau96I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
SchI GAGTC 2 cut(s) 425, 1099
ScrFI CCNGG 4 cut(s) 10, 235, 253, 1359
SduI GDGCHC 4 cut(s) 8, 365, 374, 1114
SfaNI GCATC 2 cut(s) 805, 1362
SfcI CTRYAG 4 cut(s) 465, 633, 649, 918
SinI GGWCC 2 cut(s) 163, 181
SmlI CTYRAG 3 cut(s) 301, 345, 674
SmoI CTYRAG 3 cut(s) 301, 345, 674
SphI GCATGC 1 cut(s) 948
Sse9I AATT 9 cut(s) 597, 866, 1020, 1083, 1122, 1202, 1327, 1336, 1347
SsiI CCGC 7 cut(s) 88, 341, 428, 837, 933, 1031, 1244
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 4 cut(s) 8, 233, 251, 1357
StyI CCWWGG 3 cut(s) 365, 928, 1213
TaaI ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
TaiI ACGT 1 cut(s) 203
TaqI TCGA 4 cut(s) 24, 120, 593, 765
TasI AATT 9 cut(s) 597, 866, 1020, 1083, 1122, 1202, 1327, 1336, 1347
TauI GCSGC 3 cut(s) 344, 1034, 1246
TfiI GAWTC 3 cut(s) 194, 258, 667
Tru1I TTAA 2 cut(s) 545, 675
Tru9I TTAA 2 cut(s) 545, 675
TscAI CASTG 2 cut(s) 223, 1123
TseFI GTSAC 5 cut(s) 218, 659, 718, 975, 1267
Tsp45I GTSAC 5 cut(s) 218, 659, 718, 975, 1267
TspDTI ATGAA 5 cut(s) 108, 567, 591, 634, 928
TspGWI ACGGA 2 cut(s) 163, 410
TspRI CASTG 2 cut(s) 223, 1123
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 163, 181
XapI RAATTY 3 cut(s) 866, 1122, 1336
XceI RCATGY 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.