RchiOBHm_Chr1g0348931

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001812
Physical Location & Seq
Forward (+)
41999202 .. 42003252
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57491

Sequence Viewer

Length: 1386 bp
ATGAGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAACGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACTTCCGCAATGAACCTCAAAAACCCACCACCACCGTCGAAGACGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCCGGAGATGAGGAAGATACCCGGAGATTCAAAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGCGGCTCAAGAACATGGAATGGGCCCAAGGGGCTCTGCTTTGATCTGTGGATATACGGATTACCATAGGCGACTGGAGTCATCCTTAGCGGACTTGAAGAAGAAAGAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCTTGATGGTAGTGTTGGGAAATGTTGGTTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATTGCCATTTTTTCTGATGAACTGAACCATGCGTCGATAATTGATGGTATTCGTCTTGCTGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGATTCACCTTAAGGCATTGTTATCCAGTTGCACAATGCAGAAGAAAGTTGTTGTGACTGATAGCTTGTTTAGTATGGATGGAGACTTTGCACCAATGATCGAGCTGGTGAAGCTACGCAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTTGTTTGCGGTAAAAATGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAGAGATGTTGACATATGTGTTGGCACGTTGAGTAAAGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCCTTTATATTTTCAACTGCTACACCATTACCAATTGCTGCTGCGGCCCATGCTGCTGTTATTGTCGCAAGAAAGGAAACATGGCGTAGAAAGGCAATTTGGAGTCGGGTGCAAGATTTTCGTGCTCTCACTGGAATTCCCATAAACAGTCCAATAATATCGCTTGTCGTAGGGAGCGAAGAGAAGGCTTTGAAAGCGAGCCGGAGTCTGTTGAAATCGGGTTTCCATGTGACTGCAATCAGACCCCCAACGGTGCCACCCAACTCATGCAGGCTACGAGTGACTTTGAGTGCAACACACACAAGGGCTGATATTGAGAGGTTCACAACTGCACTCTCCAATTGTGTAAATTTCCAAGAAATCGGCATCCATGGCTCCAATGGATATGCTAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

461

Amino Acids

51.13

Weight (kDa)

8.65

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 89 - 441 3.8e-48 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 137
Acc36I ACCTGC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 1240
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 6 cut(s) 88, 341, 428, 837, 933, 1031
AclI AACGTT 1 cut(s) 200
AcsI RAATTY 3 cut(s) 866, 1122, 1336
AfiI CCNNNNNNNGG 2 cut(s) 233, 320
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 6 cut(s) 47, 262, 436, 1002, 1180, 1201
AjnI CCWGG 1 cut(s) 8
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AluBI AGCT 7 cut(s) 6, 157, 268, 729, 769, 778, 917
AluI AGCT 7 cut(s) 6, 157, 268, 729, 769, 778, 917
Alw21I GWGCWC 2 cut(s) 8, 1114
Alw26I GTCTC 2 cut(s) 72, 741
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 3 cut(s) 361, 486, 1032
ApaI GGGCCC 1 cut(s) 365
ApoI RAATTY 3 cut(s) 866, 1122, 1336
AspS9I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 662, 784
AvaII GGWCC 2 cut(s) 163, 181
BaeGI GKGCMC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 1240
BanII GRGCYC 3 cut(s) 8, 365, 374
BauI CACGAG 1 cut(s) 978
BbsI GAAGAC 2 cut(s) 129, 133
Bbv12I GWGCWC 2 cut(s) 8, 1114
BbvCI CCTCAGC 1 cut(s) 861
BccI CCATC 5 cut(s) 86, 487, 596, 737, 951
BcgI CGANNNNNNTGC 2 cut(s) 1088, 1122
BciT130I CCWGG 1 cut(s) 10
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 72, 741
BfaI CTAG 1 cut(s) 1377
BfmI CTRYAG 4 cut(s) 465, 633, 649, 918
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 911
BglI GCCNNNNNGGC 2 cut(s) 369, 485
Bme1390I CCNGG 2 cut(s) 10, 253
Bme18I GGWCC 2 cut(s) 163, 181
BmgT120I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
BmiI GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
BmrFI CCNGG 2 cut(s) 10, 253
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 2 cut(s) 805, 1362
BmuI ACTGGG 2 cut(s) 28, 222
BoxI GACNNNNGTC 1 cut(s) 415
BpiI GAAGAC 2 cut(s) 129, 133
BpmI CTGGAG 1 cut(s) 434
Bpu10I CCTNAGC 3 cut(s) 336, 424, 861
BpuEI CTTGAG 2 cut(s) 322, 330
BpuMI CCSGG 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 5 cut(s) 9, 174, 365, 928, 1357
BsaWI WCCGGW 2 cut(s) 63, 233
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 320
Bse1I ACTGG 5 cut(s) 23, 228, 417, 690, 1123
Bse3DI GCAATG 2 cut(s) 97, 939
Bse8I GATNNNNATC 1 cut(s) 644
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 5 cut(s) 9, 174, 365, 928, 1357
BseGI GGATG 5 cut(s) 222, 310, 419, 748, 1353
BseJI GATNNNNATC 1 cut(s) 644
BseLI CCNNNNNNNGG 2 cut(s) 233, 320
BseMI GCAATG 2 cut(s) 97, 939
BseMII CTCAG 2 cut(s) 229, 852
BseNI ACTGG 5 cut(s) 23, 228, 417, 690, 1123
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 1302
BshFI GGCC 3 cut(s) 363, 488, 1034
BshNI GGYRCC 1 cut(s) 1240
BsiHKAI GWGCWC 2 cut(s) 8, 1114
BsiSI CCGG 4 cut(s) 64, 234, 253, 1189
BslFI GGGAC 2 cut(s) 26, 176
BslI CCNNNNNNNGG 2 cut(s) 233, 320
BsmAI GTCTC 2 cut(s) 72, 741
BsmFI GGGAC 2 cut(s) 26, 176
BsnI GGCC 3 cut(s) 363, 488, 1034
Bso31I GGTCTC 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 4 cut(s) 8, 365, 374, 1114
Bsp13I TCCGGA 1 cut(s) 233
Bsp143I GATC 2 cut(s) 381, 762
Bsp19I CCATGG 2 cut(s) 928, 1357
BspACI CCGC 6 cut(s) 88, 341, 428, 837, 933, 1031
BspANI GGCC 3 cut(s) 363, 488, 1034
BspCNI CTCAG 2 cut(s) 228, 853
BspEI TCCGGA 1 cut(s) 233
BspLI GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 1 cut(s) 911
BspT107I GGYRCC 1 cut(s) 1240
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 97, 939
BsrI ACTGG 5 cut(s) 23, 228, 417, 690, 1123
BssECI CCNNGG 5 cut(s) 9, 174, 365, 928, 1357
BssMI GATC 2 cut(s) 381, 762
BssSI CACGAG 1 cut(s) 978
BssT1I CCWWGG 3 cut(s) 365, 928, 1357
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
Bst6I CTCTTC 1 cut(s) 1161
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 5 cut(s) 53, 785, 946, 1186, 1259
BstDEI CTNAG 4 cut(s) 215, 336, 424, 861
BstDSI CCRYGG 3 cut(s) 174, 928, 1357
BstF5I GGATG 5 cut(s) 222, 310, 419, 748, 1353
BstKTI GATC 2 cut(s) 384, 765
BstMAI GTCTC 2 cut(s) 72, 741
BstMBI GATC 2 cut(s) 381, 762
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 948
BstPAI GACNNNNGTC 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 8, 251
BstSFI CTRYAG 4 cut(s) 465, 633, 649, 918
BstSLI GKGCMC 1 cut(s) 365
BstV2I GAAGAC 2 cut(s) 129, 133
BsuRI GGCC 3 cut(s) 363, 488, 1034
BtgI CCRYGG 3 cut(s) 174, 928, 1357
BtsCI GGATG 5 cut(s) 222, 310, 419, 748, 1353
BtsIMutI CAGTG 2 cut(s) 223, 1116
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 5 cut(s) 53, 785, 946, 1186, 1259
Cfr13I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
CseI GACGC 2 cut(s) 579, 993
DdeI CTNAG 4 cut(s) 215, 336, 424, 861
DpnI GATC 2 cut(s) 383, 764
DpnII GATC 2 cut(s) 381, 762
DrdI GACNNNNNNGTC 2 cut(s) 20, 137
DseDI GACNNNNNNGTC 2 cut(s) 20, 137
Eam1104I CTCTTC 1 cut(s) 1161
EarI CTCTTC 1 cut(s) 1161
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 365, 928, 1357
Eco24I GRGCYC 3 cut(s) 8, 365, 374
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 163, 181
Eco53kI GAGCTC 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 2 cut(s) 866, 1122
EcoRII CCWGG 1 cut(s) 8
EcoT14I CCWWGG 3 cut(s) 365, 928, 1357
EcoT38I GRGCYC 3 cut(s) 8, 365, 374
ErhI CCWWGG 3 cut(s) 365, 928, 1357
FaqI GGGAC 2 cut(s) 26, 176
FauI CCCGC 1 cut(s) 926
FauNDI CATATG 1 cut(s) 893
FokI GGATG 5 cut(s) 209, 297, 406, 755, 1340
FriOI GRGCYC 3 cut(s) 8, 365, 374
FspBI CTAG 1 cut(s) 1377
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 3 cut(s) 363, 488, 1034
HapII CCGG 4 cut(s) 64, 234, 253, 1189
HgaI GACGC 2 cut(s) 579, 993
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HinfI GANTC 6 cut(s) 194, 258, 416, 667, 1090, 1192
HpaII CCGG 4 cut(s) 64, 234, 253, 1189
HphI GGTGA 2 cut(s) 662, 784
Hpy166II GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy188I TCNGA 3 cut(s) 74, 574, 1229
Hpy188III TCNNGA 6 cut(s) 191, 205, 234, 282, 347, 978
Hpy8I GTNNAC 4 cut(s) 184, 308, 889, 1311
Hpy99I CGWCG 3 cut(s) 122, 131, 595
HpyAV CCTTC 2 cut(s) 464, 1165
HpyCH4III ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
HpyCH4IV ACGT 2 cut(s) 200, 905
HpyF3I CTNAG 4 cut(s) 215, 336, 424, 861
HpySE526I ACGT 2 cut(s) 200, 905
Kpn2I TCCGGA 1 cut(s) 233
Kzo9I GATC 2 cut(s) 381, 762
LmnI GCTCC 4 cut(s) 11, 992, 1161, 1367
LweI GCATC 2 cut(s) 805, 1362
MaeI CTAG 1 cut(s) 1377
MaeII ACGT 2 cut(s) 200, 905
MaeIII GTNAC 6 cut(s) 218, 659, 718, 975, 1216, 1267
MalI GATC 2 cut(s) 383, 764
MboI GATC 2 cut(s) 381, 762
MboII GAAGA 7 cut(s) 133, 134, 257, 448, 451, 718, 1178
MfeI CAATTG 2 cut(s) 1020, 1327
MhlI GDGCHC 4 cut(s) 8, 365, 374, 1114
MluCI AATT 7 cut(s) 597, 866, 1020, 1083, 1122, 1327, 1336
MlyI GAGTC 3 cut(s) 425, 1099, 1201
MmeI TCCRAC 1 cut(s) 97
MroI TCCGGA 1 cut(s) 233
MseI TTAA 2 cut(s) 545, 675
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 4 cut(s) 64, 234, 253, 1189
MspR9I CCNGG 2 cut(s) 10, 253
MunI CAATTG 2 cut(s) 1020, 1327
MvaI CCWGG 1 cut(s) 10
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 2 cut(s) 928, 1357
NdeI CATATG 1 cut(s) 893
NdeII GATC 2 cut(s) 381, 762
NlaIV GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
NmuCI GTSAC 6 cut(s) 218, 659, 718, 975, 1216, 1267
NspI RCATGY 1 cut(s) 948
PaeI GCATGC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 1161
PfeI GAWTC 3 cut(s) 194, 258, 667
PleI GAGTC 3 cut(s) 424, 1098, 1200
PpsI GAGTC 3 cut(s) 424, 1098, 1200
PshAI GACNNNNGTC 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 8
Psp1406I AACGTT 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 5 cut(s) 164, 363, 826, 1242, 1363
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 5 cut(s) 163, 181, 361, 362, 1033
PstI CTGCAG 1 cut(s) 922
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 545, 675
Sau3AI GATC 2 cut(s) 381, 762
Sau96I GGNCC 5 cut(s) 163, 181, 361, 362, 1033
SchI GAGTC 3 cut(s) 425, 1099, 1201
ScrFI CCNGG 2 cut(s) 10, 253
SduI GDGCHC 4 cut(s) 8, 365, 374, 1114
SfaNI GCATC 2 cut(s) 805, 1362
SfcI CTRYAG 4 cut(s) 465, 633, 649, 918
SinI GGWCC 2 cut(s) 163, 181
SmlI CTYRAG 3 cut(s) 301, 345, 674
SmoI CTYRAG 3 cut(s) 301, 345, 674
SphI GCATGC 1 cut(s) 948
Sse9I AATT 7 cut(s) 597, 866, 1020, 1083, 1122, 1327, 1336
SsiI CCGC 6 cut(s) 88, 341, 428, 837, 933, 1031
SspMI CTAG 1 cut(s) 1377
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 8, 251
StyI CCWWGG 3 cut(s) 365, 928, 1357
TaaI ACNGT 5 cut(s) 118, 167, 975, 1136, 1240
TaiI ACGT 2 cut(s) 203, 908
TaqI TCGA 4 cut(s) 24, 120, 593, 765
TasI AATT 7 cut(s) 597, 866, 1020, 1083, 1122, 1327, 1336
TauI GCSGC 2 cut(s) 344, 1034
TfiI GAWTC 3 cut(s) 194, 258, 667
Tru1I TTAA 2 cut(s) 545, 675
Tru9I TTAA 2 cut(s) 545, 675
TscAI CASTG 2 cut(s) 223, 1123
TseFI GTSAC 6 cut(s) 218, 659, 718, 975, 1216, 1267
Tsp45I GTSAC 6 cut(s) 218, 659, 718, 975, 1216, 1267
TspDTI ATGAA 5 cut(s) 108, 567, 591, 634, 928
TspGWI ACGGA 2 cut(s) 163, 410
TspRI CASTG 2 cut(s) 223, 1123
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 163, 181
XapI RAATTY 3 cut(s) 866, 1122, 1336
XceI RCATGY 1 cut(s) 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 1364
XspI CTAG 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.