Rh1DG212300

2-amino-3-ketobutyrate coenzyme A ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001812
Physical Location & Seq
Forward (+)
41306396 .. 41328489
22094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG212300.1

Sequence Viewer

Length: 1488 bp
ATGAGCTCCTGGGACAACTGGGTCGAACACTCCCTTGACAAACTTGAAGGCCTGCAACTCCTCCGGTCTCTCCGACCCATCTACCTCCGCAATGAACCTCAAAAACCCACCACTACCGTCGAAGACGACGACCTCCAAGTCTTCCACCAAATGCAGCTCTGGGACCGTTCCTCCGTGGAGGTCCACATTCCCGAATCAACGTTCCAGAAATGGCTCAGTGACATCCCCAGTTCCGGGGAGGAGGAAGATACCCGGGAATTCAGAAAGCTGTTGTTGTTCTCTGGAAATGATTATCTGGGCTTGAGTTCACATCCCACCATTGGAAATGCTGCTGCTAAGGATTGCCTTCTTTGTCCTACAGGGTTTGCAGCCAATATGGCCCTGATGGTAGTGCTGGGAAATGTCGGCTCTCTCTTGGCTGCTGGAAAAACACCTTTAACTAATGAAAAGATTGCCATTTTTTCTGATGAACTGAACCATGCGTCGATAATTGATGGTATTCGTCTTGCTGAACGACAAAAATCTGTAGAGATATTCATCTATAGACATTGTGACATGATTCACCTTAAGGCATTGTTATCCAGTTGCACAATGCAGAAAAAAGTTGTTGTGACTGATAGCTTGTTTAGTATGGATGGAGACTTTGCACCAATGATCGAGCTGGTGAAGCTACACAAGCAGCATGACTTTCTGTTAGTCATTGATGATGCTCATGGAACCTTCGTTTGCGGAAAAAACGGTGGTGGAGTGGCTGAGGAATTCCATTGTGAAAGAGATGTTGACATATGTGTTGGCACTTTGAGTAAGGCTGCAGGTTGCCATGGCGGGTTCATTGCATGCAGCAAAAGATGGAAGCAACTCATACAGTCACGAGGACGCTCCTTTATATTTTCAACTGCTACACCATTACCAATTGCTGCTGCGGCCCATGCTGCTGTTATTGTCGCAAGAAAGGAAACATGGCGTAGAAGGGCAATTTGGAGTCGGGTGCAAGATTTTCGTGCTCTCACTGGAATTCCCATAAACAGTCCAATAATATCGCTGGTTGTAGGGAGCGAAGAGAAGGCTTTGAAAGCGAGCCGGAGTTTGTTGAAATCGGGTTTCCATGTGACTGCAATCAGACCCCCAACAGTGCCGCCCAACTCATGCAGGCTACGAGTGACTTTGAGTGCAACACACACAAGGGCTGATATTGAGAGGTATAATTTTGGGTGGAGTCATGGAAAGGAGAAGTTGGAATCTGGAAAGCCAGATATGTCAAAAGGCTCTTTCTATGCAAATCCCATATTAGATAGACCAACAACAGATGAGGCTCTAATTCAGAGTTCCGACGACGAGGACCTCGACCTGGAGCCGGTCCTGCTCATCTCCGTAATCGGAGGTTCCATTCTAACTTCTAATAGGAAAAAGTCAGGCTTCGACACTCGGGTCTGGGTTTATGATTGTTGCAAATTTTATTTTGATATACTTGATAGTATGAGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

495

Amino Acids

55.12

Weight (kDa)

6.47

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 129 - 400 5.7e-41 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 20, 137, 1427
Acc36I ACCTGC 1 cut(s) 803
AciI CCGC 5 cut(s) 88, 729, 825, 923, 1136
AclI AACGTT 1 cut(s) 200
AcsI RAATTY 4 cut(s) 257, 758, 1014, 1451
AfiI CCNNNNNNNGG 5 cut(s) 233, 234, 320, 1348, 1354
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 4 cut(s) 47, 894, 1072, 1093
AjnI CCWGG 2 cut(s) 8, 1347
AjuI GAANNNNNNNTTGG 2 cut(s) 961, 993
AluBI AGCT 6 cut(s) 6, 157, 268, 621, 661, 670
AluI AGCT 6 cut(s) 6, 157, 268, 621, 661, 670
Alw21I GWGCWC 2 cut(s) 8, 1006
Alw26I GTCTC 3 cut(s) 72, 633, 1474
Ama87I CYCGRG 2 cut(s) 252, 1425
AoxI GGCC 3 cut(s) 49, 378, 924
ApoI RAATTY 4 cut(s) 257, 758, 1014, 1451
AspS9I GGNCC 6 cut(s) 163, 181, 379, 925, 1339, 1357
AsuC2I CCSGG 3 cut(s) 235, 253, 254
AsuHPI GGTGA 2 cut(s) 554, 676
AvaI CYCGRG 2 cut(s) 252, 1425
AvaII GGWCC 4 cut(s) 163, 181, 1339, 1357
BanII GRGCYC 1 cut(s) 8
BauI CACGAG 1 cut(s) 870
BbsI GAAGAC 2 cut(s) 129, 133
Bbv12I GWGCWC 2 cut(s) 8, 1006
BbvCI CCTCAGC 1 cut(s) 753
BccI CCATC 5 cut(s) 86, 379, 488, 629, 843
BcgI CGANNNNNNTGC 2 cut(s) 980, 1014
BciT130I CCWGG 2 cut(s) 10, 1349
BcnI CCSGG 3 cut(s) 235, 253, 254
BcoDI GTCTC 3 cut(s) 72, 633, 1474
BfmI CTRYAG 4 cut(s) 357, 525, 541, 810
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 377
Bme1390I CCNGG 5 cut(s) 10, 235, 253, 254, 1349
Bme18I GGWCC 4 cut(s) 163, 181, 1339, 1357
BmeT110I CYCGRG 2 cut(s) 252, 1425
BmgT120I GGNCC 6 cut(s) 163, 181, 379, 925, 1339, 1357
BmiI GGNNCC 4 cut(s) 164, 718, 1353, 1384
BmrFI CCNGG 5 cut(s) 10, 235, 253, 254, 1349
BmrI ACTGGG 2 cut(s) 28, 222
BmsI GCATC 1 cut(s) 697
BmuI ACTGGG 2 cut(s) 28, 222
BpiI GAAGAC 2 cut(s) 129, 133
BpmI CTGGAG 1 cut(s) 1370
Bpu10I CCTNAGC 2 cut(s) 336, 753
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 3 cut(s) 235, 253, 254
BsaBI GATNNNNATC 1 cut(s) 536
BsaI GGTCTC 1 cut(s) 72
BsaJI CCNNGG 5 cut(s) 9, 174, 234, 252, 820
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 234, 320, 1348, 1354
Bse118I RCCGGY 1 cut(s) 1354
Bse1I ACTGG 4 cut(s) 23, 228, 582, 1015
Bse3DI GCAATG 2 cut(s) 97, 831
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 2 cut(s) 10, 1349
BseDI CCNNGG 5 cut(s) 9, 174, 234, 252, 820
BseGI GGATG 3 cut(s) 222, 310, 640
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 5 cut(s) 233, 234, 320, 1348, 1354
BseMI GCAATG 2 cut(s) 97, 831
BseMII CTCAG 2 cut(s) 229, 744
BseNI ACTGG 4 cut(s) 23, 228, 582, 1015
BseRI GAGGAG 2 cut(s) 50, 254
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 3 cut(s) 51, 380, 926
BsiHKAI GWGCWC 2 cut(s) 8, 1006
BsiHKCI CYCGRG 2 cut(s) 252, 1425
BsiSI CCGG 5 cut(s) 64, 234, 253, 1081, 1355
BslFI GGGAC 2 cut(s) 26, 176
BslI CCNNNNNNNGG 5 cut(s) 233, 234, 320, 1348, 1354
BsmAI GTCTC 3 cut(s) 72, 633, 1474
BsmFI GGGAC 2 cut(s) 26, 176
BsnI GGCC 3 cut(s) 51, 380, 926
Bso31I GGTCTC 1 cut(s) 72
BsoBI CYCGRG 2 cut(s) 252, 1425
Bsp1286I GDGCHC 2 cut(s) 8, 1006
Bsp143I GATC 1 cut(s) 654
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 5 cut(s) 88, 729, 825, 923, 1136
BspANI GGCC 3 cut(s) 51, 380, 926
BspCNI CTCAG 2 cut(s) 228, 745
BspLI GGNNCC 4 cut(s) 164, 718, 1353, 1384
BspMAI CTGCAG 1 cut(s) 814
BspMI ACCTGC 1 cut(s) 803
BspTI CTTAAG 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 97, 831
BsrFI RCCGGY 1 cut(s) 1354
BsrI ACTGG 4 cut(s) 23, 228, 582, 1015
BssAI RCCGGY 1 cut(s) 1354
BssECI CCNNGG 5 cut(s) 9, 174, 234, 252, 820
BssMI GATC 1 cut(s) 654
BssSI CACGAG 1 cut(s) 870
BssT1I CCWWGG 1 cut(s) 820
Bst2BI CACGAG 1 cut(s) 870
Bst2UI CCWGG 2 cut(s) 10, 1349
Bst4CI ACNGT 6 cut(s) 118, 167, 740, 867, 1028, 1132
Bst6I CTCTTC 1 cut(s) 1053
BstAFI CTTAAG 1 cut(s) 566
BstC8I GCNNGC 4 cut(s) 53, 838, 1078, 1151
BstDEI CTNAG 3 cut(s) 215, 336, 753
BstDSI CCRYGG 2 cut(s) 174, 820
BstF5I GGATG 3 cut(s) 222, 310, 640
BstKTI GATC 1 cut(s) 657
BstMAI GTCTC 3 cut(s) 72, 633, 1474
BstMBI GATC 1 cut(s) 654
BstMWI GCNNNNNNNGC 8 cut(s) 377, 667, 676, 923, 929, 932, 1073, 1360
BstNI CCWGG 2 cut(s) 10, 1349
BstNSI RCATGY 1 cut(s) 840
BstSCI CCNGG 5 cut(s) 8, 233, 251, 252, 1347
BstSFI CTRYAG 4 cut(s) 357, 525, 541, 810
BstV2I GAAGAC 2 cut(s) 129, 133
BsuRI GGCC 3 cut(s) 51, 380, 926
BtgI CCRYGG 2 cut(s) 174, 820
BtsCI GGATG 3 cut(s) 222, 310, 640
BtsIMutI CAGTG 3 cut(s) 223, 1008, 1137
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 4 cut(s) 53, 838, 1078, 1151
Cfr10I RCCGGY 1 cut(s) 1354
Cfr13I GGNCC 6 cut(s) 163, 181, 379, 925, 1339, 1357
Cfr9I CCCGGG 1 cut(s) 252
CseI GACGC 2 cut(s) 471, 885
DdeI CTNAG 3 cut(s) 215, 336, 753
DpnI GATC 1 cut(s) 656
DpnII GATC 1 cut(s) 654
DrdI GACNNNNNNGTC 3 cut(s) 20, 137, 1427
DseDI GACNNNNNNGTC 3 cut(s) 20, 137, 1427
Eam1104I CTCTTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 1053
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 820
Eco147I AGGCCT 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 163, 181, 1339, 1357
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 2 cut(s) 252, 1425
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 1339
EcoRI GAATTC 3 cut(s) 257, 758, 1014
EcoRII CCWGG 2 cut(s) 8, 1347
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 820
FalI AAGNNNNNCTT 2 cut(s) 1400, 1432
FaqI GGGAC 2 cut(s) 26, 176
FauI CCCGC 1 cut(s) 818
FauNDI CATATG 1 cut(s) 785
FokI GGATG 3 cut(s) 209, 297, 647
FriOI GRGCYC 1 cut(s) 8
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 1370
HaeIII GGCC 3 cut(s) 51, 380, 926
HapII CCGG 5 cut(s) 64, 234, 253, 1081, 1355
HgaI GACGC 2 cut(s) 471, 885
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HinfI GANTC 5 cut(s) 194, 559, 982, 1216, 1238
HpaII CCGG 5 cut(s) 64, 234, 253, 1081, 1355
HphI GGTGA 2 cut(s) 554, 676
Hpy166II GTNNAC 3 cut(s) 184, 308, 781
Hpy188I TCNGA 7 cut(s) 74, 263, 466, 1121, 1323, 1330, 1379
Hpy188III TCNNGA 5 cut(s) 191, 205, 282, 870, 1242
Hpy8I GTNNAC 3 cut(s) 184, 308, 781
Hpy99I CGWCG 5 cut(s) 122, 131, 487, 1334, 1337
HpyAV CCTTC 5 cut(s) 41, 356, 730, 963, 1057
HpyCH4III ACNGT 6 cut(s) 118, 167, 740, 867, 1028, 1132
HpyCH4IV ACGT 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 8 cut(s) 377, 667, 676, 923, 929, 932, 1073, 1360
HpyF3I CTNAG 3 cut(s) 215, 336, 753
HpySE526I ACGT 1 cut(s) 200
Kzo9I GATC 1 cut(s) 654
LmnI GCTCC 4 cut(s) 11, 884, 1053, 1351
LweI GCATC 1 cut(s) 697
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 6 cut(s) 218, 551, 610, 867, 1108, 1159
MalI GATC 1 cut(s) 656
MboI GATC 1 cut(s) 654
MboII GAAGA 4 cut(s) 133, 134, 257, 1070
MfeI CAATTG 1 cut(s) 912
MhlI GDGCHC 2 cut(s) 8, 1006
MluCI AATT 9 cut(s) 257, 489, 758, 912, 975, 1014, 1204, 1317, 1451
MlyI GAGTC 2 cut(s) 991, 1225
MmeI TCCRAC 3 cut(s) 97, 1215, 1353
MseI TTAA 2 cut(s) 437, 567
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 5 cut(s) 64, 234, 253, 1081, 1355
MspR9I CCNGG 5 cut(s) 10, 235, 253, 254, 1349
MunI CAATTG 1 cut(s) 912
MvaI CCWGG 2 cut(s) 10, 1349
MwoI GCNNNNNNNGC 8 cut(s) 377, 667, 676, 923, 929, 932, 1073, 1360
NciI CCSGG 3 cut(s) 235, 253, 254
NcoI CCATGG 1 cut(s) 820
NdeI CATATG 1 cut(s) 785
NdeII GATC 1 cut(s) 654
NlaIV GGNNCC 4 cut(s) 164, 718, 1353, 1384
NmuCI GTSAC 6 cut(s) 218, 551, 610, 867, 1108, 1159
NspI RCATGY 1 cut(s) 840
PaeI GCATGC 1 cut(s) 840
PceI AGGCCT 1 cut(s) 51
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 1341
PfeI GAWTC 3 cut(s) 194, 559, 1238
PleI GAGTC 2 cut(s) 990, 1224
PpsI GAGTC 2 cut(s) 990, 1224
PpuMI RGGWCCY 1 cut(s) 1339
Psp124BI GAGCTC 1 cut(s) 8
Psp1406I AACGTT 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 1339
Psp6I CCWGG 2 cut(s) 8, 1347
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 8, 1347
PspN4I GGNNCC 4 cut(s) 164, 718, 1353, 1384
PspPI GGNCC 6 cut(s) 163, 181, 379, 925, 1339, 1357
PspPPI RGGWCCY 1 cut(s) 1339
PstI CTGCAG 1 cut(s) 814
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 437, 567
Sau3AI GATC 1 cut(s) 654
Sau96I GGNCC 6 cut(s) 163, 181, 379, 925, 1339, 1357
SchI GAGTC 2 cut(s) 991, 1225
ScrFI CCNGG 5 cut(s) 10, 235, 253, 254, 1349
SduI GDGCHC 2 cut(s) 8, 1006
SfaNI GCATC 1 cut(s) 697
SfcI CTRYAG 4 cut(s) 357, 525, 541, 810
SinI GGWCC 4 cut(s) 163, 181, 1339, 1357
SmaI CCCGGG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 301, 566
SmoI CTYRAG 2 cut(s) 301, 566
SphI GCATGC 1 cut(s) 840
Sse9I AATT 9 cut(s) 257, 489, 758, 912, 975, 1014, 1204, 1317, 1451
SseBI AGGCCT 1 cut(s) 51
SsiI CCGC 5 cut(s) 88, 729, 825, 923, 1136
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 51
StyD4I CCNGG 5 cut(s) 8, 233, 251, 252, 1347
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 6 cut(s) 118, 167, 740, 867, 1028, 1132
TaiI ACGT 1 cut(s) 203
TaqI TCGA 6 cut(s) 24, 120, 485, 657, 1344, 1419
TasI AATT 9 cut(s) 257, 489, 758, 912, 975, 1014, 1204, 1317, 1451
TauI GCSGC 2 cut(s) 926, 1138
TfiI GAWTC 3 cut(s) 194, 559, 1238
Tru1I TTAA 2 cut(s) 437, 567
Tru9I TTAA 2 cut(s) 437, 567
TscAI CASTG 3 cut(s) 223, 1015, 1137
TseFI GTSAC 6 cut(s) 218, 551, 610, 867, 1108, 1159
Tsp45I GTSAC 6 cut(s) 218, 551, 610, 867, 1108, 1159
TspDTI ATGAA 5 cut(s) 108, 459, 483, 526, 820
TspGWI ACGGA 2 cut(s) 163, 1360
TspMI CCCGGG 1 cut(s) 252
TspRI CASTG 3 cut(s) 223, 1015, 1137
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 4 cut(s) 163, 181, 1339, 1357
XapI RAATTY 4 cut(s) 257, 758, 1014, 1451
XceI RCATGY 1 cut(s) 840
XmaI CCCGGG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.