FvH4_7g14280

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003157
Physical Location & Seq
Forward (+)
12644207 .. 12646056
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g14280.t2

Sequence Viewer

Length: 417 bp
ATGAGCTCCAACACGACTGAGACGAACAAGGAGGAGAAGAAGAAGGGTCCTCCTCAAATTGTTAAATTGAATAAAGCTTTGGCTTTGGCTGAAAAATGGGTTAGTAACATGAGTAAACTACCGGCAGAAGATGAGTCTTTTGAGACACAGAGTCGACCTGCTAGGCTTGGACTAGGTGCTAAAGTTTCCCGTACATCCACATTTGTGCCTTCAGATGATCCCGTTGAAAGAAAGCTATATTACAAGTTGAATGCCCAAAAGAGAAAAGCTGCCAAAATCGCAGAGGAGTTAGCACCAGGTGCTGGTGATGATGACTATGAAGATAATGAAGACAGCAGAACCGGTGCGTTTGAGAAGAAAAAGAGAGCAGCTAATCCACTAACCCCACCAATAGGGAAAAAGAAAAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

139

Amino Acids

15.36

Weight (kDa)

9.7

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 19 - 136 1.4e-09 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 166
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 154
AclWI GGATC 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 195
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 302, 392
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 3 cut(s) 70, 227, 250
AhdI GACNNNNNGTC 1 cut(s) 150
AjnI CCWGG 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AleI CACNNNNGTG 1 cut(s) 203
AluBI AGCT 5 cut(s) 6, 77, 235, 269, 371
AluI AGCT 5 cut(s) 6, 77, 235, 269, 371
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 2 cut(s) 14, 137
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 269, 368
AsiGI ACCGGT 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 256, 380
BciT130I CCWGG 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 14, 137
BfaI CTAG 2 cut(s) 162, 173
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 2 cut(s) 270, 369
BlsI GCNGC 2 cut(s) 271, 370
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 47
BmeRI GACNNNNNGTC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 336
BsaWI WCCGGW 1 cut(s) 341
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 392
Bse118I RCCGGY 2 cut(s) 121, 341
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 194
BseLI CCNNNNNNNGG 2 cut(s) 302, 392
BseMII CTCAG 1 cut(s) 9
BseRI GAGGAG 3 cut(s) 42, 47, 299
BseXI GCAGC 2 cut(s) 256, 380
BshTI ACCGGT 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 2 cut(s) 122, 342
BslI CCNNNNNNNGG 2 cut(s) 302, 392
BsmAI GTCTC 2 cut(s) 14, 137
BsmBI CGTCTC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 217
BspCNI CTCAG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 212
BsrFI RCCGGY 2 cut(s) 121, 341
BssAI RCCGGY 2 cut(s) 121, 341
BssMI GATC 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 194
BstKTI GATC 1 cut(s) 220
BstMAI GTCTC 2 cut(s) 14, 137
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 278, 299
BstNI CCWGG 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 295
BstV1I GCAGC 2 cut(s) 256, 380
BstV2I GAAGAC 1 cut(s) 336
BtsCI GGATG 1 cut(s) 194
BveI ACCTGC 1 cut(s) 166
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 2 cut(s) 121, 341
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 192
CspAI ACCGGT 1 cut(s) 341
CviAII CATG 1 cut(s) 109
CviJI RGCY 8 cut(s) 6, 77, 83, 89, 166, 235, 269, 371
CviKI_1 RGCY 8 cut(s) 6, 77, 83, 89, 166, 235, 269, 371
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 1 cut(s) 18
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
DraIII CACNNNGTG 1 cut(s) 299
DriI GACNNNNNGTC 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 8
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 1 cut(s) 112
FaiI YATR 3 cut(s) 110, 238, 318
FatI CATG 1 cut(s) 108
FblI GTMKAC 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 270, 369
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 270, 369
FspBI CTAG 2 cut(s) 162, 173
GluI GCNGC 2 cut(s) 270, 369
HapII CCGG 2 cut(s) 122, 342
Hin1II CATG 1 cut(s) 112
HincII GTYRAC 1 cut(s) 155
HindII GTYRAC 1 cut(s) 155
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 2 cut(s) 134, 151
HpaII CCGG 2 cut(s) 122, 342
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 2 cut(s) 116, 155
Hpy188I TCNGA 1 cut(s) 214
Hpy8I GTNNAC 2 cut(s) 116, 155
HpyAV CCTTC 2 cut(s) 37, 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 299
HpyF3I CTNAG 1 cut(s) 18
Hsp92II CATG 1 cut(s) 112
Kzo9I GATC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 6 cut(s) 135, 171, 282, 288, 309, 355
Lsp1109I GCAGC 2 cut(s) 256, 380
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 2 cut(s) 162, 173
MaeIII GTNAC 1 cut(s) 104
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MboII GAAGA 6 cut(s) 49, 52, 140, 332, 341, 367
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 57, 65
MlyI GAGTC 2 cut(s) 143, 160
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 4 cut(s) 25, 60, 63, 277
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 2 cut(s) 122, 342
MspR9I CCNGG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 278, 299
NdeII GATC 1 cut(s) 217
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 48
OliI CACNNNNGTG 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 302
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 2 cut(s) 271, 370
PleI GAGTC 2 cut(s) 142, 159
PpsI GAGTC 2 cut(s) 142, 159
PpuMI RGGWCCY 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 47
PspPPI RGGWCCY 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 2 cut(s) 270, 369
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 2 cut(s) 143, 160
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 8
SetI ASST 8 cut(s) 8, 79, 160, 178, 237, 271, 301, 373
SexAI ACCWGGT 1 cut(s) 295
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 2 cut(s) 57, 65
SspMI CTAG 2 cut(s) 162, 173
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 295
TaqI TCGA 1 cut(s) 154
TasI AATT 2 cut(s) 57, 65
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TseI GCWGC 2 cut(s) 269, 368
TspDTI ATGAA 2 cut(s) 333, 342
Van91I CCANNNNNTGG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 154
XspI CTAG 2 cut(s) 162, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.