Rh5AG327200

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003157
Physical Location & Seq
Forward (+)
49370141 .. 49371420
1280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG327200.1

Sequence Viewer

Length: 411 bp
ATGAGCTCCAACACGACTGACACCGAGAAGGAGAAGAAGGGTCCTCCTCAAATTGTTAAATTGAATAAAGCTTTGGCATTGGCTGAAAAATGGGATAGTAACATGAGTAAATTACCAGCACAAGATGAGTCTTTTGAGACACAGAGTCGACCTGCTAGGCTTGGACTAGGTGCTAAAGTTCCCCGTGCATCCACATTTGTGCCTTCAGATGATCCCCTTGAAAGAAAGCTATATTACAAGTTGAATGCTCAAAAGAGAAAAGCTGCCAAAATTGCAGAGGAGTTGGCACCAGCTGCTGCTGATGGTGACGATGAAGATAATGAAGACAGCAGAACCAGTGCGTTCGAGAAGAAAAAGAGAGCAGCTAATCAACTCACCCCACCTTTAGGGAAAAAGAAAAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

136

Amino Acids

15.1

Weight (kDa)

9.67

Isoelectric Point (pI)

57.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 17 - 134 1.7e-09 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 148
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 3 cut(s) 64, 221, 244
AhdI GACNNNNNGTC 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 6 cut(s) 6, 71, 229, 263, 293, 365
AluI AGCT 6 cut(s) 6, 71, 229, 263, 293, 365
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 296
ApeKI GCWGC 4 cut(s) 263, 293, 296, 362
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 317, 367
AvaII GGWCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 250, 280, 283, 374
BccI CCATC 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 156, 167
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 4 cut(s) 264, 294, 297, 363
BlsI GCNGC 4 cut(s) 265, 295, 298, 364
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 2 cut(s) 42, 288
BmsI GCATC 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 386
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 2 cut(s) 36, 293
BseXI GCAGC 4 cut(s) 250, 280, 283, 374
BshNI GGYRCC 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 42, 288
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 336
BssMI GATC 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 293
BstF5I GGATG 1 cut(s) 188
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 272, 293
BstV1I GCAGC 4 cut(s) 250, 280, 283, 374
BstV2I GAAGAC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 188
BtsIMutI CAGTG 1 cut(s) 343
BveI ACCTGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 1 cut(s) 103
CviJI RGCY 8 cut(s) 6, 71, 83, 160, 229, 263, 293, 365
CviKI_1 RGCY 8 cut(s) 6, 71, 83, 160, 229, 263, 293, 365
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DriI GACNNNNNGTC 1 cut(s) 144
Eam1105I GACNNNNNGTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 41
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 1 cut(s) 106
FaiI YATR 2 cut(s) 104, 232
FatI CATG 1 cut(s) 102
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 264, 294, 297, 363
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 264, 294, 297, 363
FspBI CTAG 2 cut(s) 156, 167
GluI GCNGC 4 cut(s) 264, 294, 297, 363
Hin1II CATG 1 cut(s) 106
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 2 cut(s) 128, 145
HphI GGTGA 2 cut(s) 317, 367
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 1 cut(s) 346
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 22, 31, 213
HpyCH4V TGCA 2 cut(s) 188, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 293
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 4 cut(s) 129, 165, 303, 349
Lsp1109I GCAGC 4 cut(s) 250, 280, 283, 374
LweI GCATC 1 cut(s) 197
MaeI CTAG 2 cut(s) 156, 167
MaeIII GTNAC 2 cut(s) 98, 305
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 4 cut(s) 46, 326, 335, 361
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 51, 59, 110, 270
MlyI GAGTC 2 cut(s) 137, 154
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 3 cut(s) 54, 57, 271
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 272, 293
NdeII GATC 1 cut(s) 211
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 2 cut(s) 42, 288
NmuCI GTSAC 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 197
PctI GAATGC 1 cut(s) 250
PkrI GCNGC 4 cut(s) 265, 295, 298, 364
PleI GAGTC 2 cut(s) 136, 153
PpsI GAGTC 2 cut(s) 136, 153
PpuMI RGGWCCY 1 cut(s) 41
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 42, 288
PspPI GGNCC 1 cut(s) 41
PspPPI RGGWCCY 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 4 cut(s) 264, 294, 297, 363
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 2 cut(s) 137, 154
SduI GDGCHC 1 cut(s) 8
SetI ASST 9 cut(s) 8, 73, 154, 172, 231, 265, 295, 367, 385
SfaNI GCATC 1 cut(s) 197
SinI GGWCC 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 4 cut(s) 51, 59, 110, 270
SspMI CTAG 2 cut(s) 156, 167
SstI GAGCTC 1 cut(s) 8
TaqI TCGA 2 cut(s) 148, 345
TasI AATT 4 cut(s) 51, 59, 110, 270
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 4 cut(s) 263, 293, 296, 362
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 2 cut(s) 327, 336
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 2 cut(s) 156, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.