MD01G1060700.v1.1

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003157
Physical Location & Seq
Forward (+)
16444529 .. 16446578
2050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1060700.v1.1.491

Sequence Viewer

Length: 600 bp
ATGCCCAACAAAAAAAAATCCGCAAAGCATCAGTCTCTCTCTCTCTTTCTATTTCCTAAAATCCATCTATTTCTCTCGAACCCTCTCCAAGTCTTGGCCTTTTGCGCGCCATTGCCAAACCCAAAAGCCCGCACTCCTGCTCAGAACCCAATTCAAAGCGTCGCAATTTCTCGCTTTTCTGGGAATCGCACTGTTCGGGAAGCAATGAGCACCGAAAAGACGCCGCAGAAAAAGGAACGTCCTCAAATAGTTAAATTGGATAAAGCTTTGGATCTGGCTAAAAAATGGCTTAGTACCATGAGTGAACCTGCAGAAGATGAGCCATTTGAAATACAGGCTCGGCCTGCTAGGCTTGGACTAGGTGCTGTAGCTCCCCGCCCATCCAAGTTTGTACCTTCAAATGATCCTCTTGAAAGGAAATTACATTACAAGTTGGATGCCGCAAGAAGAAATGCTGCTAAAATTGCAGAGGAGTCCACACTAGCTGCTAAAGATGATGATGAAAATGATGATGAAGATAGCAGAACTAGTGCCTTTGCAAAGAAAAGACCAGCAGCTCCAGTAACCCCATCGTTACGGGCGAAGAAAAGGCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

200

Amino Acids

22.18

Weight (kDa)

10.07

Isoelectric Point (pI)

62.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 82 - 197 2.7e-10 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 94
AccII CGCG 1 cut(s) 107
AciI CCGC 5 cut(s) 21, 130, 224, 376, 441
AclWI GGATC 2 cut(s) 279, 398
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 2 cut(s) 295, 393
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 4 cut(s) 155, 329, 399, 413
AhlI ACTAGT 1 cut(s) 527
AluBI AGCT 4 cut(s) 266, 371, 485, 557
AluI AGCT 4 cut(s) 266, 371, 485, 557
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 2 cut(s) 279, 398
AoxI GGCC 2 cut(s) 96, 341
ApeKI GCWGC 3 cut(s) 455, 485, 554
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AspLEI GCGC 2 cut(s) 107, 109
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 3 cut(s) 442, 472, 566
BccI CCATC 3 cut(s) 72, 388, 577
BcoDI GTCTC 1 cut(s) 39
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 4 cut(s) 348, 359, 482, 528
BfmI CTRYAG 2 cut(s) 309, 366
BfuAI ACCTGC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 349
BisI GCNGC 5 cut(s) 224, 441, 456, 486, 555
BlsI GCNGC 5 cut(s) 225, 442, 457, 487, 556
BmsI GCATC 2 cut(s) 37, 427
BpmI CTGGAG 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 2 cut(s) 110, 210
BseGI GGATG 2 cut(s) 380, 442
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 2 cut(s) 110, 210
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 560
BsePI GCGCGC 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 485
BseXI GCAGC 3 cut(s) 442, 472, 566
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 98, 343
BsiHKAI GWGCWC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 39
BsnI GGCC 2 cut(s) 98, 343
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 2 cut(s) 271, 403
BspACI CCGC 5 cut(s) 21, 130, 224, 376, 441
BspANI GGCC 2 cut(s) 98, 343
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 107
BspMAI CTGCAG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 2 cut(s) 279, 398
BsrDI GCAATG 2 cut(s) 110, 210
BsrI ACTGG 1 cut(s) 560
BssHII GCGCGC 1 cut(s) 105
BssMI GATC 2 cut(s) 271, 403
BssNI GRCGYC 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 107, 130, 345
BstDEI CTNAG 2 cut(s) 141, 290
BstF5I GGATG 2 cut(s) 380, 442
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 2 cut(s) 107, 109
BstKTI GATC 2 cut(s) 274, 406
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 2 cut(s) 271, 403
BstMWI GCNNNNNNNGC 4 cut(s) 104, 344, 349, 464
BstSFI CTRYAG 2 cut(s) 309, 366
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 442, 472, 566
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 2 cut(s) 98, 343
BtsCI GGATG 2 cut(s) 380, 442
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 3 cut(s) 107, 130, 345
CfoI GCGC 2 cut(s) 107, 109
CseI GACGC 2 cut(s) 148, 229
Csp6I GTAC 2 cut(s) 294, 392
CviAII CATG 1 cut(s) 298
CviQI GTAC 2 cut(s) 294, 392
DdeI CTNAG 2 cut(s) 141, 290
DpnI GATC 2 cut(s) 273, 405
DpnII GATC 2 cut(s) 271, 403
FaeI CATG 1 cut(s) 301
FaiI YATR 1 cut(s) 299
FatI CATG 1 cut(s) 297
FauI CCCGC 2 cut(s) 137, 383
Fnu4HI GCNGC 5 cut(s) 224, 441, 456, 486, 555
FokI GGATG 2 cut(s) 367, 449
Fsp4HI GCNGC 5 cut(s) 224, 441, 456, 486, 555
FspBI CTAG 4 cut(s) 348, 359, 482, 528
GlaI GCGC 2 cut(s) 106, 108
GluI GCNGC 5 cut(s) 224, 441, 456, 486, 555
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 2 cut(s) 98, 343
HgaI GACGC 2 cut(s) 148, 229
HhaI GCGC 2 cut(s) 107, 109
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 2 cut(s) 105, 107
HinP1I GCGC 2 cut(s) 105, 107
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 184, 473
Hpy166II GTNNAC 2 cut(s) 305, 477
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 3 cut(s) 76, 197, 410
Hpy8I GTNNAC 2 cut(s) 305, 477
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 3 cut(s) 311, 467, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 344, 349, 464
HpyF3I CTNAG 2 cut(s) 141, 290
HpySE526I ACGT 1 cut(s) 238
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 2 cut(s) 105, 107
Kzo9I GATC 2 cut(s) 271, 403
LmnI GCTCC 2 cut(s) 376, 562
LpnPI CCDG 8 cut(s) 150, 165, 260, 320, 321, 357, 564, 573
Lsp1109I GCAGC 3 cut(s) 442, 472, 566
LweI GCATC 2 cut(s) 37, 427
MaeI CTAG 4 cut(s) 348, 359, 482, 528
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 2 cut(s) 562, 573
MalI GATC 2 cut(s) 273, 405
MboI GATC 2 cut(s) 271, 403
MboII GAAGA 4 cut(s) 326, 459, 527, 595
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 5 cut(s) 150, 165, 254, 419, 462
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 4 cut(s) 93, 252, 417, 463
MseI TTAA 1 cut(s) 252
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 104, 344, 349, 464
NdeII GATC 2 cut(s) 271, 403
NlaIII CATG 1 cut(s) 301
NmeAIII GCCGAG 1 cut(s) 319
PauI GCGCGC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PfeI GAWTC 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 5 cut(s) 225, 442, 457, 487, 556
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 271
PteI GCGCGC 1 cut(s) 105
RsaI GTAC 2 cut(s) 295, 393
RsaNI GTAC 2 cut(s) 294, 392
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 5 cut(s) 224, 441, 456, 486, 555
Sau3AI GATC 2 cut(s) 271, 403
SchI GAGTC 1 cut(s) 482
SduI GDGCHC 1 cut(s) 212
SetI ASST 8 cut(s) 241, 268, 310, 364, 373, 397, 487, 559
SfaNI GCATC 2 cut(s) 37, 427
SfcI CTRYAG 2 cut(s) 309, 366
SpeI ACTAGT 1 cut(s) 527
Sse9I AATT 5 cut(s) 150, 165, 254, 419, 462
SsiI CCGC 5 cut(s) 21, 130, 224, 376, 441
SspMI CTAG 4 cut(s) 348, 359, 482, 528
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 241
TaqI TCGA 1 cut(s) 77
TasI AATT 5 cut(s) 150, 165, 254, 419, 462
TauI GCSGC 2 cut(s) 226, 443
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 1 cut(s) 196
TseI GCWGC 3 cut(s) 455, 485, 554
TspDTI ATGAA 2 cut(s) 516, 528
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 94
XspI CTAG 4 cut(s) 348, 359, 482, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.