Rroxscaffold_1G00031710

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003157
Physical Location & Seq
Forward (+)
44960835 .. 44962902
2068 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00031710.1

Sequence Viewer

Length: 417 bp
ATGAGCTCCAACACGACTGACACCAAGAAGGAGAAGAAGGGTCCTCCTCAAATTGTTAAATTGAATAAAGCTTTGGCATTGGCTGAAAAATGGGTTCGTAACATGAGTAAATTACCAGCAGAAGATGAGTCTTTTGAGACACAGAGTCGACCTGCTAGGCTTGGACTAGGTGCTAAAGTTCCCCATGCATCCACATTTGTGCCTTCAGATGATCCCCTTGAAAGAAAGCTATATTACAAGTTGAATGCTCAAAAGAGAAAAGCTGCCAAAATTGCAGAGGAGTTGGCACCAGCTGCTGCAGATGGTGATGGTGACGATGAAGATAATGAAGACAGCAGAACCTGTGCGTTTGAGAAGAAAAAGAGAGCAGCTAATCCACTCACACAACCTTTAGGGAAAAAGAAAAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

138

Amino Acids

15.32

Weight (kDa)

9.65

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 17 - 136 1.5e-11 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 148
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 189
AgsI TTSAA 3 cut(s) 64, 221, 244
AhdI GACNNNNNGTC 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 6 cut(s) 6, 71, 229, 263, 293, 371
AluI AGCT 6 cut(s) 6, 71, 229, 263, 293, 371
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 2 cut(s) 296, 342
ApeKI GCWGC 4 cut(s) 263, 293, 296, 368
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 317, 323
AvaII GGWCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 250, 280, 283, 380
BccI CCATC 2 cut(s) 296, 302
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 156, 167
BfmI CTRYAG 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 4 cut(s) 264, 294, 297, 369
BlsI GCNGC 4 cut(s) 265, 295, 298, 370
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 2 cut(s) 42, 288
BmsI GCATC 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 336
BseGI GGATG 1 cut(s) 188
BseRI GAGGAG 2 cut(s) 36, 293
BseXI GCAGC 4 cut(s) 250, 280, 283, 380
BshNI GGYRCC 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 42, 288
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 286
BssMI GATC 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 293
BstF5I GGATG 1 cut(s) 188
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 272, 293
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 4 cut(s) 250, 280, 283, 380
BstV2I GAAGAC 1 cut(s) 336
BtsCI GGATG 1 cut(s) 188
BveI ACCTGC 1 cut(s) 160
CaiI CAGNNNCTG 2 cut(s) 296, 342
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 2 cut(s) 103, 185
CviJI RGCY 8 cut(s) 6, 71, 83, 160, 229, 263, 293, 371
CviKI_1 RGCY 8 cut(s) 6, 71, 83, 160, 229, 263, 293, 371
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DriI GACNNNNNGTC 1 cut(s) 144
Eam1105I GACNNNNNGTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 41
EcoT22I ATGCAT 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 2 cut(s) 106, 188
FaiI YATR 3 cut(s) 104, 186, 232
FatI CATG 2 cut(s) 102, 184
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 264, 294, 297, 369
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 264, 294, 297, 369
FspBI CTAG 2 cut(s) 156, 167
GluI GCNGC 4 cut(s) 264, 294, 297, 369
Hin1II CATG 2 cut(s) 106, 188
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 2 cut(s) 128, 145
HphI GGTGA 2 cut(s) 317, 323
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 22, 31, 213
HpyCH4V TGCA 3 cut(s) 188, 275, 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 293
Hsp92II CATG 2 cut(s) 106, 188
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 4 cut(s) 129, 165, 303, 355
Lsp1109I GCAGC 4 cut(s) 250, 280, 283, 380
LweI GCATC 1 cut(s) 197
MaeI CTAG 2 cut(s) 156, 167
MaeIII GTNAC 2 cut(s) 98, 311
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 5 cut(s) 46, 134, 332, 341, 367
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 51, 59, 110, 270
MlyI GAGTC 2 cut(s) 137, 154
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 3 cut(s) 54, 57, 271
Mph1103I ATGCAT 1 cut(s) 190
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 272, 293
NdeII GATC 1 cut(s) 211
NlaIII CATG 2 cut(s) 106, 188
NlaIV GGNNCC 2 cut(s) 42, 288
NmuCI GTSAC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 190
OliI CACNNNNGTG 1 cut(s) 197
PctI GAATGC 1 cut(s) 250
PkrI GCNGC 4 cut(s) 265, 295, 298, 370
PleI GAGTC 2 cut(s) 136, 153
PpsI GAGTC 2 cut(s) 136, 153
PpuMI RGGWCCY 1 cut(s) 41
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 42, 288
PspPI GGNCC 1 cut(s) 41
PspPPI RGGWCCY 1 cut(s) 41
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 2 cut(s) 296, 342
PvuII CAGCTG 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 4 cut(s) 264, 294, 297, 369
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 2 cut(s) 137, 154
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 1 cut(s) 197
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 197
Sse9I AATT 4 cut(s) 51, 59, 110, 270
SspMI CTAG 2 cut(s) 156, 167
SstI GAGCTC 1 cut(s) 8
TaqI TCGA 1 cut(s) 148
TasI AATT 4 cut(s) 51, 59, 110, 270
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 4 cut(s) 263, 293, 296, 368
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 333, 342
VpaK11BI GGWCC 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 2 cut(s) 156, 167
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.