MD07G1137000.v1.1

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003157
Physical Location & Seq
Reverse (-)
19975600 .. 19977572
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1137000.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGTTTATTTATCATGGTTCGATTCTATTTCGAGATTCCACAGTTTTTATGTGCGTCCTATTGTTCGGTTGAAGCCCAACAAAAGAAAACCTTTCTTTCTTCCAAAGTCTTGGCCTTTTGCACGCCATTGCCATAAAAGAACCCTAAAATCCTCACTCCTGCTCAGAACCCAATTCAAACCTTCCCAATATCTTGGCTTTTCTGGGAATTGTACCGTTAGGGCTGTGCAAGGAGTTGCAATGAGCATCGACAAGACGCCGCAGAAAAAGAAACGTCCCGGAATAGTTAAATTGGATAGAGCTTTGGATTTGGCTAAAAAATGGCTTAATACCATGAGTGAACCTGCAGAAGATGAGCCATTTGAAATACATGCTCGACCTGCTAGGCTTGGACTAGGTGCTGTAGTTCCCCGGCCATCCAAATTTGTACCTTCAAATGATCCCCTTGAAAGGAAATTACATTACAAGTTGGATGCCGCAAGAAGAAATGCTGCCAAAGTTGCGGAGGAGTCTGCACTAGCTGCTAGAGATTATGACAACGATGATGATGAAGAAGACAGCAGAACCAGTGCGTTTGCAAAGAAAAGACCGGCAGCTCCAGTAACCCCATCTCTACGGGCGAAGAAAAGGCAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

213

Amino Acids

24.26

Weight (kDa)

10.3

Isoelectric Point (pI)

64.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 95 - 210 2.6e-11 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 352, 388
AciI CCGC 3 cut(s) 260, 477, 503
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 2 cut(s) 214, 429
AfiI CCNNNNNNNGG 1 cut(s) 450
AgsI TTSAA 5 cut(s) 73, 178, 365, 435, 449
AluBI AGCT 3 cut(s) 302, 521, 596
AluI AGCT 3 cut(s) 302, 521, 596
AlwI GGATC 1 cut(s) 434
AoxI GGCC 2 cut(s) 113, 413
ApeKI GCWGC 3 cut(s) 491, 521, 593
ApoI RAATTY 1 cut(s) 422
AsuC2I CCSGG 2 cut(s) 279, 412
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 478, 508, 605
BccI CCATC 2 cut(s) 424, 616
BcnI CCSGG 2 cut(s) 279, 412
BfaI CTAG 4 cut(s) 384, 395, 518, 525
BfmI CTRYAG 2 cut(s) 345, 402
BfuAI ACCTGC 2 cut(s) 352, 388
BisI GCNGC 5 cut(s) 260, 477, 492, 522, 594
BlsI GCNGC 5 cut(s) 261, 478, 493, 523, 595
Bme1390I CCNGG 2 cut(s) 279, 412
BmrFI CCNGG 2 cut(s) 279, 412
BmsI GCATC 2 cut(s) 255, 463
BpiI GAAGAC 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 582
BpuMI CCSGG 2 cut(s) 279, 412
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 410
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse118I RCCGGY 1 cut(s) 589
Bse1I ACTGG 2 cut(s) 567, 599
Bse3DI GCAATG 2 cut(s) 127, 246
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 2 cut(s) 416, 478
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMI GCAATG 2 cut(s) 127, 246
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 2 cut(s) 567, 599
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 3 cut(s) 478, 508, 605
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 2 cut(s) 115, 415
BsiSI CCGG 3 cut(s) 279, 412, 590
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 2 cut(s) 115, 415
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 3 cut(s) 260, 477, 503
BspANI GGCC 2 cut(s) 115, 415
BspCNI CTCAG 1 cut(s) 177
BspMAI CTGCAG 1 cut(s) 349
BspMI ACCTGC 2 cut(s) 352, 388
BspPI GGATC 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 127, 246
BsrFI RCCGGY 1 cut(s) 589
BsrI ACTGG 2 cut(s) 567, 599
BssAI RCCGGY 1 cut(s) 589
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 1 cut(s) 439
BssNI GRCGYC 1 cut(s) 257
Bst4CI ACNGT 2 cut(s) 44, 217
BstACI GRCGYC 1 cut(s) 257
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 416, 478
BstKTI GATC 1 cut(s) 442
BstMBI GATC 1 cut(s) 439
BstMWI GCNNNNNNNGC 3 cut(s) 380, 500, 521
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 277, 410
BstSFI CTRYAG 2 cut(s) 345, 402
BstV1I GCAGC 3 cut(s) 478, 508, 605
BstV2I GAAGAC 1 cut(s) 561
BstXI CCANNNNNNTGG 2 cut(s) 111, 194
BsuRI GGCC 2 cut(s) 115, 415
BtsCI GGATG 2 cut(s) 416, 478
BtsIMutI CAGTG 1 cut(s) 574
BveI ACCTGC 2 cut(s) 352, 388
Cac8I GCNNGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 589
CseI GACGC 2 cut(s) 44, 265
Csp6I GTAC 2 cut(s) 213, 428
CviAII CATG 3 cut(s) 15, 334, 371
CviQI GTAC 2 cut(s) 213, 428
DdeI CTNAG 1 cut(s) 164
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 413
FaeI CATG 3 cut(s) 18, 337, 374
FaiI YATR 6 cut(s) 16, 51, 135, 335, 372, 534
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FaqI GGGAC 1 cut(s) 261
FatI CATG 3 cut(s) 14, 333, 370
Fnu4HI GCNGC 5 cut(s) 260, 477, 492, 522, 594
FokI GGATG 2 cut(s) 403, 485
Fsp4HI GCNGC 5 cut(s) 260, 477, 492, 522, 594
FspBI CTAG 4 cut(s) 384, 395, 518, 525
GluI GCNGC 5 cut(s) 260, 477, 492, 522, 594
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 2 cut(s) 115, 415
HapII CCGG 3 cut(s) 279, 412, 590
HgaI GACGC 2 cut(s) 44, 265
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 3 cut(s) 18, 337, 374
HinfI GANTC 3 cut(s) 23, 36, 509
HpaII CCGG 3 cut(s) 279, 412, 590
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 192, 441
HpyCH4III ACNGT 2 cut(s) 44, 217
HpyCH4IV ACGT 1 cut(s) 274
HpyCH4V TGCA 6 cut(s) 122, 229, 239, 347, 515, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 500, 521
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 274
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 3 cut(s) 18, 337, 374
Kzo9I GATC 1 cut(s) 439
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 9 cut(s) 173, 189, 292, 357, 393, 425, 580, 603, 612
Lsp1109I GCAGC 3 cut(s) 478, 508, 605
LweI GCATC 2 cut(s) 255, 463
MaeI CTAG 4 cut(s) 384, 395, 518, 525
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 601
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 6 cut(s) 92, 362, 495, 563, 566, 634
MluCI AATT 5 cut(s) 173, 208, 290, 422, 455
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 2 cut(s) 163, 499
MseI TTAA 2 cut(s) 288, 327
MspI CCGG 3 cut(s) 279, 412, 590
MspR9I CCNGG 2 cut(s) 279, 412
MwoI GCNNNNNNNGC 3 cut(s) 380, 500, 521
NciI CCSGG 2 cut(s) 279, 412
NdeII GATC 1 cut(s) 439
NlaIII CATG 3 cut(s) 18, 337, 374
NspI RCATGY 1 cut(s) 374
PfeI GAWTC 2 cut(s) 23, 36
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 5 cut(s) 261, 478, 493, 523, 595
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PstI CTGCAG 1 cut(s) 349
RsaI GTAC 2 cut(s) 214, 429
RsaNI GTAC 2 cut(s) 213, 428
SaqAI TTAA 2 cut(s) 288, 327
SatI GCNGC 5 cut(s) 260, 477, 492, 522, 594
Sau3AI GATC 1 cut(s) 439
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 2 cut(s) 279, 412
SfaNI GCATC 2 cut(s) 255, 463
SfcI CTRYAG 2 cut(s) 345, 402
Sse9I AATT 5 cut(s) 173, 208, 290, 422, 455
SsiI CCGC 3 cut(s) 260, 477, 503
SspMI CTAG 4 cut(s) 384, 395, 518, 525
StyD4I CCNGG 2 cut(s) 277, 410
TaaI ACNGT 2 cut(s) 44, 217
TaiI ACGT 1 cut(s) 277
TaqI TCGA 4 cut(s) 21, 32, 249, 376
TasI AATT 5 cut(s) 173, 208, 290, 422, 455
TauI GCSGC 2 cut(s) 262, 479
TfiI GAWTC 2 cut(s) 23, 36
Tru1I TTAA 2 cut(s) 288, 327
Tru9I TTAA 2 cut(s) 288, 327
TscAI CASTG 1 cut(s) 574
TseI GCWGC 3 cut(s) 491, 521, 593
TspDTI ATGAA 1 cut(s) 564
TspRI CASTG 1 cut(s) 574
XapI RAATTY 1 cut(s) 422
XceI RCATGY 1 cut(s) 374
XspI CTAG 4 cut(s) 384, 395, 518, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.