RLG00000034646

Protein of unknown function (DUF3245)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003157
Physical Location & Seq
Forward (+)
47027606 .. 47030176
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034646

Sequence Viewer

Length: 483 bp
ATGAGCTCTAACACTACTGACACCAAGAAGGAGAAGAAGGTTCCTCCTCAAATTGTTAAATTGAATAAAGCTTTGGCATTGGCTGAAAAATGGGTTAGTAACATGAGTAAATTACCAGCAGAAGATGAGTCTTTTGAGACACAGAGTCGACCTGCTAGGCTTGGACTAGGTGCTAAAGTTCCCCGTACATCCACATTTGTGCCTTCAGATGATCCCCTTGAAAGAAAGATATATTACAAGTTGAATGCTCAAAAGAGAAAAGCTGCCAAAATTGCAGAGGAGTTGGCACCAGCTGCTGCAGATGGTGATGGTGACGATGAAGATAATGAAGACAGCAGAACCATGAAAAGCTTTGGTCCTCTTCTTGTTGTAGCTGATATTCAGTACCATAACAGTGATGACTTCATTGGTTGCTCTCTCATTCTGTGGTGCCAGCCAGTTTCAAATTTCACTGGGTGGAGCGATATGACAAACTTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3245

Protein Analysis

161

Amino Acids

17.75

Weight (kDa)

5.26

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3245 PF11595 17 - 116 1.5e-08 Protein of unknown function (DUF3245)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 2 cut(s) 286, 429
AccI GTMKAC 1 cut(s) 148
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 456
AfaI GTAC 2 cut(s) 187, 386
AgsI TTSAA 4 cut(s) 64, 221, 244, 444
AhdI GACNNNNNGTC 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 6 cut(s) 6, 71, 263, 293, 351, 374
AluI AGCT 6 cut(s) 6, 71, 263, 293, 351, 374
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 296
ApeKI GCWGC 3 cut(s) 263, 293, 296
ApoI RAATTY 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 317, 323
AvaII GGWCC 1 cut(s) 356
BanI GGYRCC 2 cut(s) 286, 429
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 3 cut(s) 250, 280, 283
BccI CCATC 2 cut(s) 296, 302
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 156, 167
BfmI CTRYAG 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 3 cut(s) 264, 294, 297
BlsI GCNGC 3 cut(s) 265, 295, 298
Bme18I GGWCC 1 cut(s) 356
BmeRI GACNNNNNGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 3 cut(s) 42, 288, 431
BmrI ACTGGG 1 cut(s) 462
BmuI ACTGGG 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 336
Bse1I ACTGG 2 cut(s) 437, 457
BseGI GGATG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 437, 457
BseRI GAGGAG 2 cut(s) 36, 293
BseXI GCAGC 3 cut(s) 250, 280, 283
BshNI GGYRCC 2 cut(s) 286, 429
BsiHKAI GWGCWC 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 42, 288, 431
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 286, 429
BsrI ACTGG 2 cut(s) 437, 457
BssMI GATC 1 cut(s) 211
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 434
BstF5I GGATG 1 cut(s) 188
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 272, 293
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 250, 280, 283
BstV2I GAAGAC 1 cut(s) 336
BtsCI GGATG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 400, 450
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 434
CaiI CAGNNNCTG 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 2 cut(s) 186, 385
CviAII CATG 2 cut(s) 103, 343
CviJI RGCY 9 cut(s) 6, 71, 83, 160, 263, 293, 351, 374, 436
CviKI_1 RGCY 9 cut(s) 6, 71, 83, 160, 263, 293, 351, 374, 436
CviQI GTAC 2 cut(s) 186, 385
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 456
DriI GACNNNNNGTC 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 366
Eam1105I GACNNNNNGTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 2 cut(s) 106, 346
FaiI YATR 5 cut(s) 104, 232, 344, 390, 467
FatI CATG 2 cut(s) 102, 342
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 264, 294, 297
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 264, 294, 297
FspBI CTAG 2 cut(s) 156, 167
GluI GCNGC 3 cut(s) 264, 294, 297
Hin1II CATG 2 cut(s) 106, 346
HincII GTYRAC 1 cut(s) 149
HindII GTYRAC 1 cut(s) 149
HindIII AAGCTT 2 cut(s) 69, 349
HinfI GANTC 2 cut(s) 128, 145
HphI GGTGA 2 cut(s) 317, 323
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 22, 31, 213
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4V TGCA 2 cut(s) 275, 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 293
Hsp92II CATG 2 cut(s) 106, 346
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 459
LpnPI CCDG 6 cut(s) 129, 165, 303, 438, 446, 450
Lsp1109I GCAGC 3 cut(s) 250, 280, 283
MaeI CTAG 2 cut(s) 156, 167
MaeIII GTNAC 2 cut(s) 98, 311
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 5 cut(s) 46, 134, 332, 341, 353
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 5 cut(s) 51, 59, 110, 270, 445
MlyI GAGTC 2 cut(s) 137, 154
MnlI CCTC 4 cut(s) 54, 57, 271, 369
MseI TTAA 1 cut(s) 57
MslI CAYNNNNRTG 2 cut(s) 197, 393
MspA1I CMGCKG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 272, 293
NdeII GATC 1 cut(s) 211
NlaIII CATG 2 cut(s) 106, 346
NlaIV GGNNCC 3 cut(s) 42, 288, 431
NmuCI GTSAC 1 cut(s) 311
OliI CACNNNNGTG 1 cut(s) 197
PctI GAATGC 1 cut(s) 250
PkrI GCNGC 3 cut(s) 265, 295, 298
PleI GAGTC 2 cut(s) 136, 153
PpsI GAGTC 2 cut(s) 136, 153
Psp124BI GAGCTC 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 42, 288, 431
PspPI GGNCC 1 cut(s) 356
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 2 cut(s) 187, 386
RsaNI GTAC 2 cut(s) 186, 385
RseI CAYNNNNRTG 2 cut(s) 197, 393
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 3 cut(s) 264, 294, 297
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 2 cut(s) 137, 154
SduI GDGCHC 1 cut(s) 8
SetI ASST 9 cut(s) 8, 42, 73, 154, 172, 265, 295, 353, 376
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 2 cut(s) 197, 393
Sse9I AATT 5 cut(s) 51, 59, 110, 270, 445
SspMI CTAG 2 cut(s) 156, 167
SstI GAGCTC 1 cut(s) 8
TaaI ACNGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 148
TasI AATT 5 cut(s) 51, 59, 110, 270, 445
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 2 cut(s) 400, 457
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 3 cut(s) 263, 293, 296
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 4 cut(s) 333, 342, 359, 394
TspRI CASTG 2 cut(s) 400, 457
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 2 cut(s) 156, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.