FvH4_7g22200

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004142
Physical Location & Seq
Forward (+)
17554386 .. 17555743
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22200.t1

Sequence Viewer

Length: 507 bp
ATGGCACAATCGTCCTCTGCAACCTCTGCATCTTCACGAACTGCCTCATTCATATTGAGGATATTGGCCTTCGCTCTCTCGCTCATATCACTCATAGTGCTCGCCACCAATACTGTAATTGTCACTGATTACGATTATAATGAGGCAAAGTTTCGTTTTCAGGATTTTTATGCCTACCGATACATGCTTGCTACGATCGTTATTGGTACCGCATACAGCCTCCTGCAACTCGTGGCATCAATCTGTAATGCAATATCTGGTGGTGAGGGCATGCTCGTTCTTGCTTTCTTTGGTGACAAGTTTATATCCTATGTGATGGCCACCGGAGCTGCTGCTGGATTTGGTGTAACTGTAGACCTATCACGAGTCACGGGAGGCACGGGTTTAGACATGAGCAATTTCTTTGAGAAGGCATATGCATCAGCTAGTCTCCTCCTCCTCGCTTTCATTTGCACTGCGTTGCTATCTATTATCTCTGCCTTTGCACTTCCAAGGCGATCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

169

Amino Acids

17.9

Weight (kDa)

7.73

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 11 - 151 4.1e-20 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 354
AciI CCGC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 318
AfaI GTAC 1 cut(s) 208
AluBI AGCT 2 cut(s) 329, 425
AluI AGCT 2 cut(s) 329, 425
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 434
AoxI GGCC 2 cut(s) 66, 318
ApeKI GCWGC 2 cut(s) 329, 332
Asp718I GGTACC 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 275, 305
BalI TGGCCA 1 cut(s) 320
BanI GGYRCC 1 cut(s) 206
BauI CACGAG 2 cut(s) 230, 363
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 316, 319
BccI CCATC 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 2 cut(s) 330, 333
BlsI GCNGC 2 cut(s) 331, 334
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 3 cut(s) 38, 245, 428
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 491
BseRI GAGGAG 3 cut(s) 422, 425, 428
BseXI GCAGC 2 cut(s) 316, 319
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 68, 320
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 324
BsmAI GTCTC 1 cut(s) 434
BsnI GGCC 2 cut(s) 68, 320
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 195, 497
BspACI CCGC 1 cut(s) 210
BspANI GGCC 2 cut(s) 68, 320
BspLI GGNNCC 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 2 cut(s) 195, 497
BssSI CACGAG 2 cut(s) 230, 363
BssT1I CCWWGG 1 cut(s) 491
Bst2BI CACGAG 2 cut(s) 230, 363
Bst4CI ACNGT 2 cut(s) 115, 352
BstAPI GCANNNNNTGC 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 102, 189, 272
BstKTI GATC 2 cut(s) 198, 500
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 2 cut(s) 195, 497
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 2 cut(s) 26, 326
BstNSI RCATGY 2 cut(s) 187, 274
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 316, 319
BsuRI GGCC 2 cut(s) 68, 320
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 123, 453
Cac8I GCNNGC 3 cut(s) 102, 189, 272
Csp6I GTAC 1 cut(s) 207
CviAII CATG 3 cut(s) 184, 271, 391
CviJI RGCY 5 cut(s) 68, 219, 320, 329, 425
CviKI_1 RGCY 5 cut(s) 68, 219, 320, 329, 425
CviQI GTAC 1 cut(s) 207
DpnI GATC 2 cut(s) 197, 499
DpnII GATC 2 cut(s) 195, 497
EaeI YGGCCR 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 491
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 3 cut(s) 187, 274, 394
FatI CATG 3 cut(s) 183, 270, 390
FauNDI CATATG 1 cut(s) 415
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 330, 333
Fsp4HI GCNGC 2 cut(s) 330, 333
FspBI CTAG 1 cut(s) 426
GluI GCNGC 2 cut(s) 330, 333
HaeIII GGCC 2 cut(s) 68, 320
HapII CCGG 1 cut(s) 324
Hin1II CATG 3 cut(s) 187, 274, 394
HinfI GANTC 1 cut(s) 366
HpaII CCGG 1 cut(s) 324
HphI GGTGA 2 cut(s) 275, 305
Hpy166II GTNNAC 1 cut(s) 355
Hpy188III TCNNGA 3 cut(s) 36, 161, 363
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 79, 403
HpyCH4III ACNGT 2 cut(s) 115, 352
HpyCH4V TGCA 7 cut(s) 20, 29, 226, 251, 419, 453, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 326
Hsp92II CATG 3 cut(s) 187, 274, 394
KpnI GGTACC 1 cut(s) 210
Kzo9I GATC 2 cut(s) 195, 497
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 5 cut(s) 146, 236, 243, 321, 337
Lsp1109I GCAGC 2 cut(s) 316, 319
LweI GCATC 3 cut(s) 38, 245, 428
MaeI CTAG 1 cut(s) 426
MaeIII GTNAC 4 cut(s) 121, 293, 346, 367
MalI GATC 2 cut(s) 197, 499
MboI GATC 2 cut(s) 195, 497
MboII GAAGA 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 117, 397
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 375
Mox20I TGGCCA 1 cut(s) 320
Mph1103I ATGCAT 1 cut(s) 421
MscI TGGCCA 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 320
MspI CCGG 1 cut(s) 324
MwoI GCNNNNNNNGC 2 cut(s) 26, 326
NdeI CATATG 1 cut(s) 415
NdeII GATC 2 cut(s) 195, 497
NlaIII CATG 3 cut(s) 187, 274, 394
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 3 cut(s) 121, 293, 367
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 2 cut(s) 187, 274
PaeI GCATGC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 331, 334
Ple19I CGATCG 1 cut(s) 198
PleI GAGTC 1 cut(s) 374
PpsI GAGTC 1 cut(s) 374
PsiI TTATAA 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 208
PvuI CGATCG 1 cut(s) 198
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
SatI GCNGC 2 cut(s) 330, 333
Sau3AI GATC 2 cut(s) 195, 497
SchI GAGTC 1 cut(s) 375
SduI GDGCHC 1 cut(s) 102
SetI ASST 4 cut(s) 26, 331, 360, 427
SfaNI GCATC 3 cut(s) 38, 245, 428
SfcI CTRYAG 1 cut(s) 351
SphI GCATGC 1 cut(s) 274
Sse9I AATT 2 cut(s) 117, 397
SsiI CCGC 1 cut(s) 210
SspMI CTAG 1 cut(s) 426
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 2 cut(s) 115, 352
TasI AATT 2 cut(s) 117, 397
TscAI CASTG 2 cut(s) 130, 460
TseFI GTSAC 3 cut(s) 121, 293, 367
TseI GCWGC 2 cut(s) 329, 332
Tsp45I GTSAC 3 cut(s) 121, 293, 367
TspDTI ATGAA 2 cut(s) 40, 436
TspRI CASTG 2 cut(s) 130, 460
XceI RCATGY 2 cut(s) 187, 274
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.