FvH4_7g22201

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004142
Physical Location & Seq
Forward (+)
17556398 .. 17557195
798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22201.t1

Sequence Viewer

Length: 510 bp
ATGGAAACTCACACACCAGTAGTGCTTCCCACTAGATCTTCAAAGTATACTAATATAGGGCTGAGAGTCCTCACCTCAGCTCTTCTCTTCATGTCACTGGTCATCCTTGTCACCAATGTTAAGTCAAATTATACGGGCTTGAAGACGACACTTCACTTCTATGATCGTGTCGGATACCGGTACATGGCTGCCGCAGTTGTTATTGGACTTGCCTATTCGATAATGCAAACTGTAACAGCAGTTATCCGCATCAATAAGTACAGTACTCAAGGAAACATCTTACTTGACTTCTACGGTGACAAGGTTGTATCAACTATAATAGCTACAGGAGCAGTTGCAGGCTTTGCTATGACCGGAGAACTTCAGAGAGACGCTGGAAAAGGCTGGACTGAATATTATCACAAATATTTCGGAATGTCGCATGCAGCAAATGGACTTGCCTTCCTCGGATTTGTCTGCGCTTTCATATCATCTGTTTTCTCGTCATATGCACTCCCAAAGAGATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

170

Amino Acids

18.61

Weight (kDa)

9.65

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 13 - 153 7.6e-19 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 47
AciI CCGC 2 cut(s) 192, 247
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 3 cut(s) 182, 260, 265
AfiI CCNNNNNNNGG 1 cut(s) 184
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 2 cut(s) 42, 142
AluBI AGCT 2 cut(s) 80, 323
AluI AGCT 2 cut(s) 80, 323
Alw26I GTCTC 1 cut(s) 363
ApeKI GCWGC 2 cut(s) 188, 425
AsiGI ACCGGT 1 cut(s) 177
AspLEI GCGC 1 cut(s) 461
AsuHPI GGTGA 3 cut(s) 64, 103, 308
BbsI GAAGAC 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 175, 437
BciVI GTATCC 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 167
BglII AGATCT 1 cut(s) 35
BisI GCNGC 3 cut(s) 189, 192, 426
BlsI GCNGC 3 cut(s) 190, 193, 427
BmcAI AGTACT 1 cut(s) 265
BmsI GCATC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 2 cut(s) 177, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 17, 102
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 53, 90
BseNI ACTGG 2 cut(s) 17, 102
BseXI GCAGC 2 cut(s) 175, 437
BshTI ACCGGT 1 cut(s) 177
BsiSI CCGG 2 cut(s) 178, 354
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
Bsp143I GATC 2 cut(s) 35, 163
BspACI CCGC 2 cut(s) 192, 247
BspCNI CTCAG 2 cut(s) 54, 89
BspQI GCTCTTC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 2 cut(s) 17, 102
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 2 cut(s) 35, 163
BssNAI GTATAC 1 cut(s) 48
Bst1107I GTATAC 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 232, 263, 296
Bst6I CTCTTC 2 cut(s) 87, 92
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 340, 423
BstDEI CTNAG 2 cut(s) 62, 76
BstF5I GGATG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 2 cut(s) 38, 166
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 2 cut(s) 35, 163
BstMWI GCNNNNNNNGC 2 cut(s) 329, 344
BstNSI RCATGY 1 cut(s) 425
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 175, 437
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BstZ17I GTATAC 1 cut(s) 48
BsuI GTATCC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 340, 423
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 177
CseI GACGC 1 cut(s) 380
Csp6I GTAC 3 cut(s) 181, 259, 264
CspAI ACCGGT 1 cut(s) 177
CviAII CATG 3 cut(s) 91, 184, 422
CviJI RGCY 7 cut(s) 61, 80, 138, 188, 323, 342, 384
CviKI_1 RGCY 7 cut(s) 61, 80, 138, 188, 323, 342, 384
CviQI GTAC 3 cut(s) 181, 259, 264
DdeI CTNAG 2 cut(s) 62, 76
DpnI GATC 2 cut(s) 37, 165
DpnII GATC 2 cut(s) 35, 163
Eam1104I CTCTTC 2 cut(s) 87, 92
EarI CTCTTC 2 cut(s) 87, 92
Eco57I CTGAAG 1 cut(s) 347
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 3 cut(s) 94, 187, 425
FatI CATG 3 cut(s) 90, 183, 421
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 3 cut(s) 189, 192, 426
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 3 cut(s) 189, 192, 426
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 460
GluI GCNGC 3 cut(s) 189, 192, 426
HapII CCGG 2 cut(s) 178, 354
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 461
Hin1II CATG 3 cut(s) 94, 187, 425
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HinfI GANTC 1 cut(s) 66
HpaII CCGG 2 cut(s) 178, 354
HphI GGTGA 3 cut(s) 64, 103, 308
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 4 cut(s) 173, 366, 413, 449
Hpy8I GTNNAC 1 cut(s) 48
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 232, 263, 296
HpyCH4V TGCA 4 cut(s) 226, 338, 425, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 344
HpyF3I CTNAG 2 cut(s) 62, 76
Hsp92II CATG 3 cut(s) 94, 187, 425
HspAI GCGC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 35, 163
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 8 cut(s) 30, 83, 191, 312, 324, 360, 367, 370
Lsp1109I GCAGC 2 cut(s) 175, 437
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 4 cut(s) 93, 109, 232, 296
MalI GATC 2 cut(s) 37, 165
MboI GATC 2 cut(s) 35, 163
MboII GAAGA 4 cut(s) 30, 74, 79, 154
MflI RGATCY 1 cut(s) 35
MluCI AATT 1 cut(s) 127
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 3 cut(s) 80, 85, 455
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 1 cut(s) 159
MspI CCGG 2 cut(s) 178, 354
MwoI GCNNNNNNNGC 2 cut(s) 329, 344
NdeI CATATG 1 cut(s) 487
NdeII GATC 2 cut(s) 35, 163
NlaIII CATG 3 cut(s) 94, 187, 425
NmuCI GTSAC 3 cut(s) 93, 109, 296
NspI RCATGY 1 cut(s) 425
PaeI GCATGC 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 87
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 3 cut(s) 190, 193, 427
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 3 cut(s) 182, 260, 265
RsaNI GTAC 3 cut(s) 181, 259, 264
RseI CAYNNNNRTG 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 3 cut(s) 189, 192, 426
Sau3AI GATC 2 cut(s) 35, 163
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 1 cut(s) 75
SetI ASST 4 cut(s) 77, 82, 306, 325
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SphI GCATGC 1 cut(s) 425
Sse9I AATT 1 cut(s) 127
SsiI CCGC 2 cut(s) 192, 247
SspI AATATT 2 cut(s) 395, 407
SspMI CTAG 1 cut(s) 33
TaaI ACNGT 3 cut(s) 232, 263, 296
TaqI TCGA 1 cut(s) 218
TasI AATT 1 cut(s) 127
TatI WGTACW 2 cut(s) 258, 263
TauI GCSGC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 3 cut(s) 93, 109, 296
TseI GCWGC 2 cut(s) 188, 425
Tsp45I GTSAC 3 cut(s) 93, 109, 296
TspDTI ATGAA 2 cut(s) 79, 454
TspRI CASTG 1 cut(s) 102
XceI RCATGY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 1 cut(s) 33
ZrmI AGTACT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.