MD01G1126800.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004142
Physical Location & Seq
Forward (+)
23864218 .. 23865309
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1126800.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGAATCACAAACACCAGCACCAGCACTCCCTTCTGTCAGAGCTTCCCAGTTTGTCATTCTTGGGTTGAGGGTCCTCACCTCCATTTTTCTAGTTGTGTCACTCGCCATCCTCCTCTCCAATGTTTTGTCTGGAGATCAAGATCACAAAATACACTTTTATGACTACGTGCCATACCGATACATGGCTGCCGCAATTGTTATTGGAATTGCATATTCAGTCTTCCAAACTGTAGTTGCAGTTATCCGCATAAGAAGAGAAAATCATAGGAGCATGTTACTCGACTTCTATGGTGACAAGGTTATATCAAATGTAGTAGCTACTGGAGCTGTTGCAGGATTTGTTATGACCGCACAACTACAGAAACAATGGGGCGACATAGTAAAGATTGACATTGTCCACAAATATATCAAAAGGTCACATGCAGCAGATGGACTTGTCTTCCTTGCCTTCATCTGCACTTTCATATTATCCGTCCTCTCTTCCTATGCACTTCCCAAGAAGGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

171

Amino Acids

18.9

Weight (kDa)

9.52

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 17 - 153 3.8e-17 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 192, 247, 351
AfiI CCNNNNNNNGG 1 cut(s) 184
AluBI AGCT 3 cut(s) 44, 320, 329
AluI AGCT 3 cut(s) 44, 320, 329
ApeKI GCWGC 2 cut(s) 188, 425
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 70, 305
AvaII GGWCC 1 cut(s) 73
BbsI GAAGAC 2 cut(s) 214, 433
BbvI GCAGC 2 cut(s) 175, 437
BccI CCATC 2 cut(s) 116, 425
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BfaI CTAG 2 cut(s) 92, 511
BfmI CTRYAG 2 cut(s) 231, 359
BisI GCNGC 3 cut(s) 189, 192, 426
BlsI GCNGC 3 cut(s) 190, 193, 427
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 74
BmrI ACTGGG 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 43
BpiI GAAGAC 2 cut(s) 214, 433
BpmI CTGGAG 2 cut(s) 153, 345
BsaAI YACGTR 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 49, 328
Bse8I GATNNNNATC 1 cut(s) 141
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 49, 328
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 2 cut(s) 175, 437
BsgI GTGCAG 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 184
Bsp143I GATC 2 cut(s) 136, 142
BspACI CCGC 3 cut(s) 192, 247, 351
BspLI GGNNCC 1 cut(s) 74
BsrI ACTGG 2 cut(s) 49, 328
BssMI GATC 2 cut(s) 136, 142
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 2 cut(s) 250, 487
BstBAI YACGTR 1 cut(s) 169
BstF5I GGATG 1 cut(s) 108
BstKTI GATC 2 cut(s) 139, 145
BstMBI GATC 2 cut(s) 136, 142
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNSI RCATGY 2 cut(s) 277, 425
BstSFI CTRYAG 2 cut(s) 231, 359
BstV1I GCAGC 2 cut(s) 175, 437
BstV2I GAAGAC 2 cut(s) 214, 433
BtsCI GGATG 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 73
CviAII CATG 3 cut(s) 184, 274, 422
CviJI RGCY 4 cut(s) 44, 188, 320, 329
CviKI_1 RGCY 4 cut(s) 44, 188, 320, 329
DpnI GATC 2 cut(s) 138, 144
DpnII GATC 2 cut(s) 136, 142
Eam1104I CTCTTC 2 cut(s) 250, 487
EarI CTCTTC 2 cut(s) 250, 487
Eco47I GGWCC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 73
FaeI CATG 3 cut(s) 187, 277, 425
FatI CATG 3 cut(s) 183, 273, 421
Fnu4HI GCNGC 3 cut(s) 189, 192, 426
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 3 cut(s) 189, 192, 426
FspBI CTAG 2 cut(s) 92, 511
GluI GCNGC 3 cut(s) 189, 192, 426
GsuI CTGGAG 2 cut(s) 153, 345
Hin1II CATG 3 cut(s) 187, 277, 425
HinfI GANTC 1 cut(s) 5
HphI GGTGA 2 cut(s) 70, 305
Hpy166II GTNNAC 2 cut(s) 400, 508
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 2 cut(s) 132, 140
Hpy8I GTNNAC 2 cut(s) 400, 508
HpyAV CCTTC 3 cut(s) 42, 460, 496
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 6 cut(s) 212, 239, 335, 425, 459, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 3 cut(s) 187, 277, 425
Kzo9I GATC 2 cut(s) 136, 142
LmnI GCTCC 2 cut(s) 270, 326
LpnPI CCDG 6 cut(s) 30, 36, 62, 117, 309, 321
Lsp1109I GCAGC 2 cut(s) 175, 437
MaeI CTAG 2 cut(s) 92, 511
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 4 cut(s) 99, 276, 293, 417
MalI GATC 2 cut(s) 138, 144
MboI GATC 2 cut(s) 136, 142
MboII GAAGA 4 cut(s) 214, 267, 433, 474
MfeI CAATTG 1 cut(s) 195
MluCI AATT 2 cut(s) 195, 207
MnlI CCTC 6 cut(s) 63, 86, 91, 122, 125, 488
MslI CAYNNNNRTG 1 cut(s) 159
MunI CAATTG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 326
NdeII GATC 2 cut(s) 136, 142
NlaIII CATG 3 cut(s) 187, 277, 425
NlaIV GGNNCC 1 cut(s) 74
NmuCI GTSAC 3 cut(s) 99, 293, 417
NspI RCATGY 2 cut(s) 277, 425
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 395
PkrI GCNGC 3 cut(s) 190, 193, 427
Ppu21I YACGTR 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 73
PspPPI RGGWCCY 1 cut(s) 73
PsyI GACNNNGTC 1 cut(s) 395
RseI CAYNNNNRTG 1 cut(s) 159
SatI GCNGC 3 cut(s) 189, 192, 426
Sau3AI GATC 2 cut(s) 136, 142
Sau96I GGNCC 1 cut(s) 73
SetI ASST 8 cut(s) 46, 83, 171, 303, 322, 331, 419, 507
SfcI CTRYAG 2 cut(s) 231, 359
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 2 cut(s) 195, 207
SsiI CCGC 3 cut(s) 192, 247, 351
SspMI CTAG 2 cut(s) 92, 511
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 282
TasI AATT 2 cut(s) 195, 207
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 1 cut(s) 5
TseFI GTSAC 3 cut(s) 99, 293, 417
TseI GCWGC 2 cut(s) 188, 425
Tsp45I GTSAC 3 cut(s) 99, 293, 417
TspDTI ATGAA 2 cut(s) 442, 454
TspGWI ACGGA 1 cut(s) 463
Tth111I GACNNNGTC 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 73
XceI RCATGY 2 cut(s) 277, 425
XspI CTAG 2 cut(s) 92, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.