RchiOBHm_Chr1g0366591

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004142
Physical Location & Seq
Forward (+)
57058585 .. 57059446
862 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59112

Sequence Viewer

Length: 507 bp
ATGGAAACTCACACACAAGTAGTGCTTTCCCCTAGATCTTCAAAGTATGCTAATATTGAGCTGAGAGTCCTCACCGCAACTTTGTTCTTCATGTCATTGGTCATCCTTGTCACCAATGTTAGGTCAAATTATCTGTACACGAAGAAGACACTTCACTTCTATGATCGTGTCAGATACCGGTACATGGCTGCCGCGGTTGTTATTGGACTTGCGTATTCAATCTTGCAGACTGTAATTGCAGTTATCCGCATCAAAAAGTACGGTCAAGGAAACATCTTACTTGACTTCTACGGTGACAAGGTTGTATCAACTATAATAGCTACTGGAGCTGTTGCAGGGTTTGCTATGACTGGAGAACTTCAGAGGGTCATTGGAAAAGATTGGAGTGATTACTATTACAAATATTTCAGAATGTCTCACGCAGCAAATGGACTTGCCTTCCTCGGATTTGTCTGCGCTTTCATATCATCCGTCATCTCGTCATGTGCACTCCCTAAGAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

168

Amino Acids

18.9

Weight (kDa)

9.78

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 14 - 152 3.7e-18 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 194
AciI CCGC 4 cut(s) 75, 192, 194, 247
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 3 cut(s) 137, 182, 260
AfiI CCNNNNNNNGG 2 cut(s) 120, 184
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 2 cut(s) 42, 219
AluBI AGCT 3 cut(s) 61, 320, 329
AluI AGCT 3 cut(s) 61, 320, 329
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 1 cut(s) 420
Alw44I GTGCAC 1 cut(s) 486
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 188, 422
AsiGI ACCGGT 1 cut(s) 177
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 3 cut(s) 64, 103, 305
BaeGI GKGCMC 1 cut(s) 490
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 490
BbvI GCAGC 2 cut(s) 175, 434
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 1 cut(s) 33
BglII AGATCT 1 cut(s) 35
BisI GCNGC 3 cut(s) 189, 192, 423
BlsI GCNGC 3 cut(s) 190, 193, 424
BmsI GCATC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 2 cut(s) 345, 372
BsaJI CCNNGG 2 cut(s) 192, 442
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 184
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 328, 355
BseDI CCNNGG 2 cut(s) 192, 442
BseGI GGATG 2 cut(s) 102, 467
BseLI CCNNNNNNNGG 2 cut(s) 120, 184
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 328, 355
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 2 cut(s) 175, 434
Bsh1236I CGCG 1 cut(s) 194
BshTI ACCGGT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 490
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 120, 184
BsmAI GTCTC 1 cut(s) 420
Bsp1286I GDGCHC 1 cut(s) 490
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 2 cut(s) 35, 163
BspACI CCGC 4 cut(s) 75, 192, 194, 247
BspCNI CTCAG 1 cut(s) 54
BspFNI CGCG 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 2 cut(s) 328, 355
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 192, 442
BssMI GATC 2 cut(s) 35, 163
Bst4CI ACNGT 3 cut(s) 232, 263, 293
BstAPI GCANNNNNTGC 1 cut(s) 341
BstAUI TGTACA 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 62, 495
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 102, 467
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 2 cut(s) 38, 166
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 2 cut(s) 35, 163
BstMWI GCNNNNNNNGC 2 cut(s) 326, 341
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 175, 434
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 102, 467
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 177
Cfr42I CCGCGG 1 cut(s) 195
Csp6I GTAC 3 cut(s) 136, 181, 259
CspAI ACCGGT 1 cut(s) 177
CviAII CATG 3 cut(s) 91, 184, 483
CviJI RGCY 4 cut(s) 61, 188, 320, 329
CviKI_1 RGCY 4 cut(s) 61, 188, 320, 329
CviQI GTAC 3 cut(s) 136, 181, 259
DdeI CTNAG 2 cut(s) 62, 495
DpnI GATC 2 cut(s) 37, 165
DpnII GATC 2 cut(s) 35, 163
Eco57I CTGAAG 1 cut(s) 344
FaeI CATG 3 cut(s) 94, 187, 486
FaiI YATR 8 cut(s) 48, 92, 162, 185, 314, 347, 464, 484
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 3 cut(s) 90, 183, 482
Fnu4HI GCNGC 3 cut(s) 189, 192, 423
FokI GGATG 2 cut(s) 89, 454
Fsp4HI GCNGC 3 cut(s) 189, 192, 423
FspBI CTAG 1 cut(s) 33
GlaI GCGC 1 cut(s) 457
GluI GCNGC 3 cut(s) 189, 192, 423
GsuI CTGGAG 2 cut(s) 345, 372
HapII CCGG 1 cut(s) 178
HhaI GCGC 1 cut(s) 458
Hin1II CATG 3 cut(s) 94, 187, 486
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 1 cut(s) 66
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 64, 103, 305
Hpy166II GTNNAC 2 cut(s) 138, 488
Hpy188I TCNGA 4 cut(s) 173, 363, 410, 446
Hpy8I GTNNAC 2 cut(s) 138, 488
HpyAV CCTTC 1 cut(s) 448
HpyCH4III ACNGT 3 cut(s) 232, 263, 293
HpyCH4V TGCA 4 cut(s) 226, 239, 335, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 341
HpyF3I CTNAG 2 cut(s) 62, 495
Hsp92II CATG 3 cut(s) 94, 187, 486
HspAI GCGC 1 cut(s) 456
KspI CCGCGG 1 cut(s) 195
Kzo9I GATC 2 cut(s) 35, 163
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 4 cut(s) 191, 309, 321, 336
Lsp1109I GCAGC 2 cut(s) 175, 434
LweI GCATC 1 cut(s) 258
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 109, 293
MalI GATC 2 cut(s) 37, 165
MboI GATC 2 cut(s) 35, 163
MboII GAAGA 4 cut(s) 30, 79, 154, 157
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 2 cut(s) 127, 234
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 3 cut(s) 80, 357, 452
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 2 cut(s) 326, 341
NdeII GATC 2 cut(s) 35, 163
NlaIII CATG 3 cut(s) 94, 187, 486
NmuCI GTSAC 2 cut(s) 109, 293
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 3 cut(s) 190, 193, 424
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 3 cut(s) 137, 182, 260
RsaNI GTAC 3 cut(s) 136, 181, 259
RseI CAYNNNNRTG 1 cut(s) 159
SacII CCGCGG 1 cut(s) 195
SatI GCNGC 3 cut(s) 189, 192, 423
Sau3AI GATC 2 cut(s) 35, 163
SchI GAGTC 1 cut(s) 75
SduI GDGCHC 1 cut(s) 490
SetI ASST 5 cut(s) 63, 125, 303, 322, 331
SfaNI GCATC 1 cut(s) 258
Sfr303I CCGCGG 1 cut(s) 195
SgrBI CCGCGG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 2 cut(s) 127, 234
SsiI CCGC 4 cut(s) 75, 192, 194, 247
SspI AATATT 2 cut(s) 55, 404
SspMI CTAG 1 cut(s) 33
TaaI ACNGT 3 cut(s) 232, 263, 293
TasI AATT 2 cut(s) 127, 234
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 194
TseFI GTSAC 2 cut(s) 109, 293
TseI GCWGC 2 cut(s) 188, 422
Tsp45I GTSAC 2 cut(s) 109, 293
TspDTI ATGAA 2 cut(s) 79, 451
TspGWI ACGGA 1 cut(s) 460
VneI GTGCAC 1 cut(s) 486
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.