Rh1BG300400

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004142
Physical Location & Seq
Forward (+)
43511659 .. 43512670
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG300400.1

Sequence Viewer

Length: 495 bp
ATGGCACAATTGTCCTCTGCATCTTCACGAACTGCCTCATTCATATTGAGGATCTTGGCCTTCGCTTTCTCACTCATATCACTCATAGTGCTCGCCACGAATACTGTAATTGCCATTGATTCCGATTATGATGAGGCCAAAGTTCATTTTCAGGATTTTTATGCCTACCGATACATGCTTGCTACGATCGTTATTGGTACCGCATATAGCCTCCTGCAGCTCGTGGCATCAATCTGTACTGCAGTATCTGGTCGCGAGGGCATGCTTGTACTTGCTTTCTTTGGTGACAAGTTCATGTCCTATGTGCTAGCCACCGGAGCTGCTGCTGGATTTGGTGTAACTGTAGACCTAAAACGAATCACGGATGCCGAGGGTTTAGAGATGAGCAAGTTCTATGAGAAGGCATATGCATCAGCTAGTCTCCTCCTCCTCGCTTTTATTTGCACTGCTGTGTTATCTATTATCTCTGCCTTTATACTCCCAAGGATTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

164

Amino Acids

17.7

Weight (kDa)

5.72

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 148 2.2e-21 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 255
AciI CCGC 1 cut(s) 201
AclWI GGATC 1 cut(s) 59
AfaI GTAC 3 cut(s) 199, 238, 270
AleI CACNNNNGTG 1 cut(s) 449
AluBI AGCT 3 cut(s) 220, 320, 416
AluI AGCT 3 cut(s) 220, 320, 416
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 425
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 2 cut(s) 57, 135
ApeKI GCWGC 3 cut(s) 217, 320, 323
Asp718I GGTACC 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 296
AsuNHI GCTAGC 1 cut(s) 307
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BanI GGYRCC 1 cut(s) 197
BauI CACGAG 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 3 cut(s) 229, 307, 310
BcoDI GTCTC 1 cut(s) 425
BfaI CTAG 2 cut(s) 308, 417
BfmI CTRYAG 3 cut(s) 215, 240, 342
BisI GCNGC 3 cut(s) 218, 321, 324
BlsI GCNGC 3 cut(s) 219, 322, 325
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 4 cut(s) 29, 236, 355, 419
BmtI GCTAGC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 369, 482
BsaWI WCCGGW 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 369, 482
BseGI GGATG 1 cut(s) 370
BseRI GAGGAG 3 cut(s) 413, 416, 419
BseXI GCAGC 3 cut(s) 229, 307, 310
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 59, 137
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 425
BsnI GGCC 2 cut(s) 59, 137
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 2 cut(s) 51, 186
Bsp68I TCGCGA 1 cut(s) 255
BspACI CCGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 59, 137
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 199
BspMAI CTGCAG 2 cut(s) 219, 244
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 369, 482
BssMI GATC 2 cut(s) 51, 186
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 106, 343
BstC8I GCNNGC 4 cut(s) 93, 180, 263, 309
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 2 cut(s) 54, 189
BstMAI GTCTC 1 cut(s) 425
BstMBI GATC 2 cut(s) 51, 186
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNSI RCATGY 2 cut(s) 178, 265
BstSFI CTRYAG 3 cut(s) 215, 240, 342
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 3 cut(s) 229, 307, 310
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 2 cut(s) 59, 137
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 1 cut(s) 444
BtuMI TCGCGA 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 93, 180, 263, 309
CaiI CAGNNNCTG 1 cut(s) 248
Csp6I GTAC 3 cut(s) 198, 237, 269
CviAII CATG 3 cut(s) 175, 262, 295
CviJI RGCY 7 cut(s) 59, 137, 210, 220, 311, 320, 416
CviKI_1 RGCY 7 cut(s) 59, 137, 210, 220, 311, 320, 416
CviQI GTAC 3 cut(s) 198, 237, 269
DpnI GATC 2 cut(s) 53, 188
DpnII GATC 2 cut(s) 51, 186
Eco130I CCWWGG 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 482
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 3 cut(s) 178, 265, 298
FatI CATG 3 cut(s) 174, 261, 294
FauNDI CATATG 1 cut(s) 406
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 3 cut(s) 218, 321, 324
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 3 cut(s) 218, 321, 324
FspBI CTAG 2 cut(s) 308, 417
GluI GCNGC 3 cut(s) 218, 321, 324
HaeIII GGCC 2 cut(s) 59, 137
HapII CCGG 1 cut(s) 315
Hin1II CATG 3 cut(s) 178, 265, 298
HinfI GANTC 3 cut(s) 119, 357, 487
HpaII CCGG 1 cut(s) 315
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 27, 152, 254
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 2 cut(s) 70, 394
HpyCH4III ACNGT 2 cut(s) 106, 343
HpyCH4V TGCA 5 cut(s) 20, 217, 242, 410, 444
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 178, 265, 298
KpnI GGTACC 1 cut(s) 201
Kzo9I GATC 2 cut(s) 51, 186
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 5 cut(s) 137, 227, 234, 312, 328
Lsp1109I GCAGC 3 cut(s) 229, 307, 310
LweI GCATC 4 cut(s) 29, 236, 355, 419
MaeI CTAG 2 cut(s) 308, 417
MaeIII GTNAC 2 cut(s) 284, 337
MalI GATC 2 cut(s) 53, 188
MboI GATC 2 cut(s) 51, 186
MboII GAAGA 1 cut(s) 15
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 1 cut(s) 93
MluCI AATT 2 cut(s) 8, 108
Mph1103I ATGCAT 1 cut(s) 412
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 8
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeI CATATG 1 cut(s) 406
NdeII GATC 2 cut(s) 51, 186
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 3 cut(s) 178, 265, 298
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 284
NruI TCGCGA 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 2 cut(s) 178, 265
OliI CACNNNNGTG 1 cut(s) 449
PaeI GCATGC 1 cut(s) 265
PfeI GAWTC 3 cut(s) 119, 357, 487
PkrI GCNGC 3 cut(s) 219, 322, 325
Ple19I CGATCG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 199
PstI CTGCAG 2 cut(s) 219, 244
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 1 cut(s) 51
PvuI CGATCG 1 cut(s) 189
RruI TCGCGA 1 cut(s) 255
RsaI GTAC 3 cut(s) 199, 238, 270
RsaNI GTAC 3 cut(s) 198, 237, 269
RseI CAYNNNNRTG 1 cut(s) 449
SatI GCNGC 3 cut(s) 218, 321, 324
Sau3AI GATC 2 cut(s) 51, 186
SduI GDGCHC 1 cut(s) 93
SetI ASST 4 cut(s) 222, 322, 351, 418
SfaNI GCATC 4 cut(s) 29, 236, 355, 419
SfcI CTRYAG 3 cut(s) 215, 240, 342
SmiMI CAYNNNNRTG 1 cut(s) 449
SphI GCATGC 1 cut(s) 265
Sse9I AATT 2 cut(s) 8, 108
SsiI CCGC 1 cut(s) 201
SspMI CTAG 2 cut(s) 308, 417
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 2 cut(s) 106, 343
TasI AATT 2 cut(s) 8, 108
TatI WGTACW 2 cut(s) 236, 268
TfiI GAWTC 3 cut(s) 119, 357, 487
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 217, 320, 323
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 3 cut(s) 31, 134, 283
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 451
XceI RCATGY 2 cut(s) 178, 265
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 308, 417
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.