MD00G1097500.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003891
Physical Location & Seq
Reverse (-)
20399985 .. 20405925
5941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1097500.v1.1.491

Sequence Viewer

Length: 3129 bp
ATGGAACAGTTGCAATTAACTGCAGAATCTCAGCAGAAGGAGGATCATTCTTCTAAGAACAACCATAAAAGTGATAACACACTTGAGGGCTCTGGCAGCCCTCAAAGTCGAAAGATATCAGCTAGATGGAACCCGGAGGAAGCATGCAGGCCTGATATTGATGAAGCACCCGTGTTTTATCCAACTATTGAGGAGTTTGAAGATACACTTGGTTACATGGCAACGATCCGCCCACTGGCCGAATCTTATGGTATATGCAGGATTGTCCCTCCACCTTCCTGGACCCCTCCCTGCCCTCTTAAGGAGAAAGAGGTGTGGGAACATGCCAAATTTTCTACACGCATTCAGCAAGTTGACTTGCTGCAAAACAGAGAGGCCATGAGAAAGAAAAGTAGAAGTCGGAAGCGTAAACGGAGGAGGAACTCAAGAATGGGAGCCAGAAGACATTCGGAAGCTAATGCTGCTACTGAGGCTGATAAGAGGTTTGGGTTCCATTCAGGATCAGACTTTACATTTGAAGAGTTTCAGAAACATGCTGCTACTTTTAAGGAGAGTTACTTTGGAACAAAGGATGTCAAGGAGGGTTCAACTTGTGGTGAAACTAAAAGCAAAACATGGGAACCCTCTGTGGAGGACATCGAAGGTGAATACTGGCGAATAGTTGAGACACCAACAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGTTTTCCTAAGGCATCATCAACCGGAATTGATTTGGAGAAGTATGCAATGTCAGGTTGGAATCTAAACAACTTCCCACGTCTGCCTGGTTCTGCACTGTGTTTTGAAGCAAGCGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGCATGTTGAGGACCACCACCTGTATTCGCTAAATTGTCTGCACTGGGGTGACCCAAAAGTATGGTATGGAGTCTCTGGAAGCCATGCTATGGATTTGGAACGTACAATGAAAGCGTATTTGCCTGATCTGTTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTACAGTTTTAAAGTCTGAAGGTGTACCTGTGCATCGAGCCGTCCAGCATTCTGGGGAATTTGTTCTTACCTTCCCGCGGGCATACCATTCTGGATTTAATTGCGGCTTCAACTGTGCTGAGGCAGTGAACGTGGCCCCTGCTGATTGGCTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGTGTCGTAAGACATCAATTTCACACGACAAATTGTTAATGGGATCAGCTCGGGAAGCTGTACATGCCCTCTGGGAAAAATCAGTTCTTGGAAAGGAAACTACAAAAAATATGAGCTGGCAAAGTGTTTGTGGCAAAGATGGTCTACTCACCAGAGCAGTTAAGATACGAGTGCGAATGGAGGAGGAAAGACTAGATCGTCTTCCAATTTGTATGAAGTTGAAAAAGATGGAAAGAGACTTTGATTTGAACAATGAGAGAGAATGTTTTTCTTGCTTCTATGACTTGCACTTATCTGCCTCCGGCTGCAAATGCTCTCCTGACCAATTTTCATGTCTCAAACATGCAAAGCACCTTTGTTCATGTCACATCAGTCATAGATATGTCCTTCAACGCCACACTATCAATGAATTAAATATGCTGGTTGAAGCATTGGAAGGGAAAGTAGAGGCCATAAAAGTATGGGCATCCAAGGACCACGGAGGAGACGGTACTGATACATATACAACTAAGCTGGATGAAGAAAGTGGCATGCCTCGTAAAAGAATCAAATCTTGTGAGCCAAGGGAACCTTCACCTTGCTGCCCGGTTTCAGAAGAGAAGGTGAACATAAATGCATCTAGCAGTTCGTCCAGTCAGGTTTCTTCTGCAGTAGTCCAGTCAGGGTCACAGCATGGAACTTCTAGTTTAAGTACGTCTCCCATCACTATGGATATTCAAAATGACGATCAAACTCTGGTAAAGAATGATGAAGCAAAGACGGGGATGGAATGCTTTGATCTGAATCTTAATTATATGTCTGAGGAACAAGAAAGCAGGACGATGCACACATCTGATCACTGTGATAACAAGGCTATCACAATTGAGGAGGAGACCTCTACATCGGTGTCTAGTCAAAAGAAAGTATGTAGTTCAAATGTAGCTAGAGAACTAGACATGATGAAAGTTGATGATGACTGTAATGTATCTGCTCTGACAGTTCTGAACAATGACCACCCTGCCGGTTCAAGGGACATCAGGAATAACTGTGCGAGTGAGGGCAACAAGTTGTTGGGTGTAAATATATCTGTGACTGACCAAAGCTATCAATTACAAAAGTTGATTCCTTCCGTTGAGCCTATTGATTTTGGAGCTGTTGTCTCTGGCAAGTTGTGGTGCAGTAAGCAGGCCATATATCCAAAAGGGTATAGAAGCCGAGTTAGGTTTTATAGTGTGCTTGACCCAACAAAAGTTTGTAGCTATATTTCAGAAGTTCTCGATGCTGGGCTTCTTGGTCCTCTATTCAAGGTTAGTTTGGAAGATTTCCCAGGTGAGGTTTTCGCAAATGTATCGGCAGAGAAGTGCTGGGAAATGGTACTACAGAGACTACACCAAGAAATAAACAGACGGAGTAGTGTAGGGGAAAGCGGACTGCCCCATATACAGCCATTGCAGAGCATTAATGGGCTCGAAATGTTTGGATTTCTCTCCCAACCCATTGCTGAGGCTATTGAGGCTCTTGATCCTGACCATCAATGCGTGGACTACTGGAATCACAGGCGCATGGTCCCATCAATGTCTTGCAGGGTTAGTGAAATCAAGCAGCATTCATTCGAACAAAGTTGCTGTCTAGGAGAAATGGAGACGAAAGCTTTTGGTGTCAGTTTGACAAAGCCGGATCAGGATTGTTCATCGGCGGAAGATCATCATTCAACTGAAGAGACGCAGTTAGTACTACGAAGACTACTAAAGAAGGCAGATTCAGAAGAGTTAAGTACATTACAGAGAGTAATCTGCAGTAAGTCACAAAGTGCAAAATGGAGGGTGGCATTCGCATCAATGATCGAAGAGATCCAGAAACATGTAGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1043

Amino Acids

117.3

Weight (kDa)

5.63

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 57 - 90 7.1e-16 jmjN domain
JmjC PF02373 291 - 407 9.3e-43 JmjC domain, hydroxylase
zf-C5HC2 PF02928 514 - 564 1.2e-12 C5HC2 zinc finger
FYRN PF05964 794 - 845 2.9e-12 F/Y-rich N-terminus
FYRC PF05965 850 - 938 2.6e-20 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1473
AccB7I CCANNNNNTGG 1 cut(s) 997
AccI GTMKAC 1 cut(s) 1420
AccII CGCG 1 cut(s) 1165
AciI CCGC 6 cut(s) 229, 1163, 1165, 1191, 2682, 2951
AclWI GGATC 7 cut(s) 51, 220, 508, 1327, 2771, 2940, 3100
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 2 cut(s) 329, 1145
AcuI CTGAAG 2 cut(s) 1125, 2991
AdeI CACNNNGTG 1 cut(s) 3065
AfaI GTAC 8 cut(s) 1012, 1113, 1338, 1768, 1969, 2631, 2988, 3031
AfiI CCNNNNNNNGG 6 cut(s) 235, 301, 997, 1164, 2283, 2587
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 3115
AjiI CACGTC 1 cut(s) 812
AjnI CCWGG 3 cut(s) 278, 817, 2581
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 1831, 1863
AleI CACNNNNGTG 1 cut(s) 954
Alw21I GWGCWC 1 cut(s) 1266
AlwI GGATC 7 cut(s) 51, 220, 508, 1327, 2771, 2940, 3100
Ama87I CYCGRG 2 cut(s) 853, 1326
AoxI GGCC 6 cut(s) 149, 237, 375, 1221, 1725, 2442
ApeKI GCWGC 7 cut(s) 96, 361, 461, 536, 1581, 1857, 2857
ApoI RAATTY 2 cut(s) 329, 1145
ArsI GACNNNNNNTTYG 4 cut(s) 497, 529, 2866, 2898
AseI ATTAAT 1 cut(s) 2715
Asp700I GAANNNNTTC 3 cut(s) 445, 522, 1149
AspLEI GCGC 1 cut(s) 2817
AspS9I GGNCC 6 cut(s) 282, 919, 1222, 1750, 2549, 2821
AsuC2I CCSGG 2 cut(s) 134, 1862
AsuHPI GGTGA 7 cut(s) 608, 656, 968, 1417, 1842, 1891, 2597
AsuII TTCGAA 1 cut(s) 2868
AvaI CYCGRG 2 cut(s) 853, 1326
AvaII GGWCC 5 cut(s) 282, 919, 1750, 2549, 2821
AxyI CCTNAGG 1 cut(s) 741
BanII GRGCYC 3 cut(s) 92, 1266, 2724
BbsI GAAGAC 3 cut(s) 448, 1469, 3001
Bbv12I GWGCWC 1 cut(s) 1266
BbvCI CCTCAGC 2 cut(s) 1206, 2757
BbvI GCAGC 7 cut(s) 108, 348, 448, 523, 1568, 1844, 2869
BccI CCATC 7 cut(s) 120, 1409, 1498, 1985, 2035, 2793, 2833
BceAI ACGGC 1 cut(s) 1112
BcgI CGANNNNNNTGC 2 cut(s) 2586, 2620
BciT130I CCWGG 3 cut(s) 280, 819, 2583
BclI TGATCA 1 cut(s) 2110
BcnI CCSGG 2 cut(s) 134, 1862
BfmI CTRYAG 6 cut(s) 21, 1089, 1267, 1923, 2633, 3049
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 8 cut(s) 97, 362, 462, 537, 1192, 1582, 1858, 2858
BlsI GCNGC 8 cut(s) 98, 363, 463, 538, 1193, 1583, 1859, 2859
BmcAI AGTACT 1 cut(s) 2988
Bme1390I CCNGG 5 cut(s) 134, 280, 819, 1862, 2583
Bme18I GGWCC 5 cut(s) 282, 919, 1750, 2549, 2821
BmeT110I CYCGRG 2 cut(s) 853, 1326
BmgBI CACGTC 1 cut(s) 812
BmgT120I GGNCC 6 cut(s) 282, 919, 1222, 1750, 2549, 2821
BmiI GGNNCC 8 cut(s) 131, 284, 436, 491, 621, 1224, 1845, 2823
BmrFI CCNGG 5 cut(s) 134, 280, 819, 1862, 2583
BmrI ACTGGG 1 cut(s) 961
BmsI GCATC 7 cut(s) 755, 1129, 1751, 1901, 2088, 2524, 3098
BmuI ACTGGG 1 cut(s) 961
BpiI GAAGAC 3 cut(s) 448, 1469, 3001
BplI GAGNNNNNCTC 2 cut(s) 2135, 2167
Bpu10I CCTNAGC 2 cut(s) 1206, 2757
Bpu14I TTCGAA 1 cut(s) 2868
BpuEI CTTGAG 2 cut(s) 104, 409
BpuMI CCSGG 2 cut(s) 134, 1862
BsaBI GATNNNNATC 1 cut(s) 2058
BsaI GGTCTC 1 cut(s) 2141
BsaJI CCNNGG 6 cut(s) 869, 1163, 1746, 1753, 1838, 2581
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 4 cut(s) 849, 879, 2397, 2427
Bsc4I CCNNNNNNNGG 6 cut(s) 235, 301, 997, 1164, 2283, 2587
Bse118I RCCGGY 1 cut(s) 2276
Bse1I ACTGG 7 cut(s) 240, 656, 956, 1074, 1908, 1933, 2807
Bse21I CCTNAGG 1 cut(s) 741
Bse3DI GCAATG 3 cut(s) 786, 2702, 2751
Bse8I GATNNNNATC 1 cut(s) 2058
BseBI CCWGG 3 cut(s) 280, 819, 2583
BseDI CCNNGG 6 cut(s) 869, 1163, 1746, 1753, 1838, 2581
BseGI GGATG 5 cut(s) 577, 891, 1742, 1798, 2046
BseJI GATNNNNATC 1 cut(s) 2058
BseLI CCNNNNNNNGG 6 cut(s) 235, 301, 997, 1164, 2283, 2587
BseMI GCAATG 3 cut(s) 786, 2702, 2751
BseMII CTCAG 6 cut(s) 44, 459, 1091, 1197, 2067, 2748
BseNI ACTGG 7 cut(s) 240, 656, 956, 1074, 1908, 1933, 2807
BseRI GAGGAG 6 cut(s) 206, 430, 1472, 1773, 2156, 2159
BseXI GCAGC 7 cut(s) 108, 348, 448, 523, 1568, 1844, 2869
BseYI CCCAGC 2 cut(s) 2537, 2619
BsgI GTGCAG 3 cut(s) 810, 932, 2452
Bsh1236I CGCG 1 cut(s) 1165
BshFI GGCC 6 cut(s) 151, 239, 377, 1223, 1727, 2444
BsiHKAI GWGCWC 1 cut(s) 1266
BsiHKCI CYCGRG 2 cut(s) 853, 1326
BsiSI CCGG 6 cut(s) 134, 756, 1578, 1862, 2277, 2930
BslFI GGGAC 4 cut(s) 251, 851, 2300, 2807
BslI CCNNNNNNNGG 6 cut(s) 235, 301, 997, 1164, 2283, 2587
BsmBI CGTCTC 4 cut(s) 1755, 1977, 2891, 2969
BsmFI GGGAC 4 cut(s) 251, 851, 2300, 2807
BsmI GAATGC 5 cut(s) 342, 1135, 2051, 2860, 3083
BsnI GGCC 6 cut(s) 151, 239, 377, 1223, 1727, 2444
Bso31I GGTCTC 1 cut(s) 2141
BsoBI CYCGRG 2 cut(s) 853, 1326
Bsp119I TTCGAA 1 cut(s) 2868
Bsp1286I GDGCHC 3 cut(s) 92, 1266, 2724
Bsp1407I TGTACA 1 cut(s) 1336
Bsp19I CCATGG 1 cut(s) 869
BspACI CCGC 6 cut(s) 229, 1163, 1165, 1191, 2682, 2951
BspANI GGCC 6 cut(s) 151, 239, 377, 1223, 1727, 2444
BspCNI CTCAG 6 cut(s) 43, 460, 1090, 1198, 2068, 2749
BspFNI CGCG 1 cut(s) 1165
BspLI GGNNCC 8 cut(s) 131, 284, 436, 491, 621, 1224, 1845, 2823
BspMAI CTGCAG 3 cut(s) 25, 1927, 3053
BspPI GGATC 7 cut(s) 51, 220, 508, 1327, 2771, 2940, 3100
BspT104I TTCGAA 1 cut(s) 2868
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 2141
BsrDI GCAATG 3 cut(s) 786, 2702, 2751
BsrFI RCCGGY 1 cut(s) 2276
BsrGI TGTACA 1 cut(s) 1336
BsrI ACTGG 7 cut(s) 240, 656, 956, 1074, 1908, 1933, 2807
BssAI RCCGGY 1 cut(s) 2276
BssECI CCNNGG 6 cut(s) 869, 1163, 1746, 1753, 1838, 2581
BssT1I CCWWGG 3 cut(s) 869, 1746, 1838
Bst2UI CCWGG 3 cut(s) 280, 819, 2583
Bst6I CTCTTC 5 cut(s) 513, 1866, 2967, 3015, 3096
BstAFI CTTAAG 1 cut(s) 299
BstAUI TGTACA 1 cut(s) 1336
BstBI TTCGAA 1 cut(s) 2868
BstC8I GCNNGC 7 cut(s) 145, 149, 844, 1167, 1394, 1808, 2442
BstDEI CTNAG 9 cut(s) 30, 54, 468, 741, 1077, 1206, 1785, 2076, 2757
BstDSI CCRYGG 3 cut(s) 869, 1163, 1753
BstEII GGTNACC 1 cut(s) 956
BstF5I GGATG 5 cut(s) 577, 891, 1742, 1798, 2046
BstFNI CGCG 1 cut(s) 1165
BstHHI GCGC 1 cut(s) 2817
BstMWI GCNNNNNNNGC 7 cut(s) 96, 461, 470, 1331, 1340, 1587, 2768
BstNI CCWGG 3 cut(s) 280, 819, 2583
BstNSI RCATGY 8 cut(s) 147, 326, 536, 914, 1343, 1622, 1810, 3119
BstPI GGTNACC 1 cut(s) 956
BstSCI CCNGG 5 cut(s) 132, 278, 817, 1860, 2581
BstSFI CTRYAG 6 cut(s) 21, 1089, 1267, 1923, 2633, 3049
BstUI CGCG 1 cut(s) 1165
BstV1I GCAGC 7 cut(s) 108, 348, 448, 523, 1568, 1844, 2869
BstV2I GAAGAC 3 cut(s) 448, 1469, 3001
BstX2I RGATCY 1 cut(s) 3105
BstXI CCANNNNNNTGG 4 cut(s) 279, 969, 1139, 1984
BstYI RGATCY 1 cut(s) 3105
Bsu36I CCTNAGG 1 cut(s) 741
BsuRI GGCC 6 cut(s) 151, 239, 377, 1223, 1727, 2444
BtgI CCRYGG 3 cut(s) 869, 1163, 1753
BtrI CACGTC 1 cut(s) 812
BtsCI GGATG 5 cut(s) 577, 891, 1742, 1798, 2046
BtsI GCAGTG 2 cut(s) 1218, 1283
BtsIMutI CAGTG 7 cut(s) 233, 827, 949, 1218, 1276, 1283, 2113
Cac8I GCNNGC 7 cut(s) 145, 149, 844, 1167, 1394, 1808, 2442
CfoI GCGC 1 cut(s) 2817
Cfr10I RCCGGY 1 cut(s) 2276
Cfr13I GGNCC 6 cut(s) 282, 919, 1222, 1750, 2549, 2821
Cfr42I CCGCGG 1 cut(s) 1166
CseI GACGC 1 cut(s) 2986
Csp6I GTAC 8 cut(s) 1011, 1112, 1337, 1767, 1968, 2630, 2987, 3030
CviQI GTAC 8 cut(s) 1011, 1112, 1337, 1767, 1968, 2630, 2987, 3030
DdeI CTNAG 9 cut(s) 30, 54, 468, 741, 1077, 1206, 1785, 2076, 2757
DraI TTTAAA 1 cut(s) 1098
DraIII CACNNNGTG 1 cut(s) 3065
DrdI GACNNNNNNGTC 1 cut(s) 1473
DseDI GACNNNNNNGTC 1 cut(s) 1473
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 5 cut(s) 513, 1866, 2967, 3015, 3096
EarI CTCTTC 5 cut(s) 513, 1866, 2967, 3015, 3096
EciI GGCGGA 2 cut(s) 218, 2966
Ecl136II GAGCTC 1 cut(s) 1264
Eco130I CCWWGG 3 cut(s) 869, 1746, 1838
Eco147I AGGCCT 1 cut(s) 151
Eco24I GRGCYC 3 cut(s) 92, 1266, 2724
Eco31I GGTCTC 1 cut(s) 2141
Eco32I GATATC 2 cut(s) 117, 850
Eco47I GGWCC 5 cut(s) 282, 919, 1750, 2549, 2821
Eco53kI GAGCTC 1 cut(s) 1264
Eco57I CTGAAG 2 cut(s) 1125, 2991
Eco81I CCTNAGG 1 cut(s) 741
Eco88I CYCGRG 2 cut(s) 853, 1326
Eco91I GGTNACC 1 cut(s) 956
EcoICRI GAGCTC 1 cut(s) 1264
EcoO65I GGTNACC 1 cut(s) 956
EcoRII CCWGG 3 cut(s) 278, 817, 2581
EcoRV GATATC 2 cut(s) 117, 850
EcoT14I CCWWGG 3 cut(s) 869, 1746, 1838
EcoT22I ATGCAT 1 cut(s) 1894
EcoT38I GRGCYC 3 cut(s) 92, 1266, 2724
ErhI CCWWGG 3 cut(s) 869, 1746, 1838
Esp3I CGTCTC 4 cut(s) 1755, 1977, 2891, 2969
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 1831, 1863
FaqI GGGAC 4 cut(s) 251, 851, 2300, 2807
FauI CCCGC 2 cut(s) 1158, 1170
FbaI TGATCA 1 cut(s) 2110
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 8 cut(s) 97, 362, 462, 537, 1192, 1582, 1858, 2858
FokI GGATG 5 cut(s) 584, 898, 1729, 1805, 2053
FriOI GRGCYC 3 cut(s) 92, 1266, 2724
Fsp4HI GCNGC 8 cut(s) 97, 362, 462, 537, 1192, 1582, 1858, 2858
GlaI GCGC 1 cut(s) 2816
GluI GCNGC 8 cut(s) 97, 362, 462, 537, 1192, 1582, 1858, 2858
GsaI CCCAGC 2 cut(s) 2541, 2623
HaeIII GGCC 6 cut(s) 151, 239, 377, 1223, 1727, 2444
HapII CCGG 6 cut(s) 134, 756, 1578, 1862, 2277, 2930
HgaI GACGC 1 cut(s) 2986
HhaI GCGC 1 cut(s) 2817
Hin6I GCGC 1 cut(s) 2815
HinP1I GCGC 1 cut(s) 2815
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 2904
HinfI GANTC 9 cut(s) 26, 242, 793, 978, 1821, 2059, 2377, 2806, 3014
HpaII CCGG 6 cut(s) 134, 756, 1578, 1862, 2277, 2930
HphI GGTGA 7 cut(s) 608, 656, 968, 1417, 1842, 1891, 2597
Hpy166II GTNNAC 8 cut(s) 355, 410, 694, 1112, 1216, 1421, 1882, 2797
Hpy8I GTNNAC 8 cut(s) 355, 410, 694, 1112, 1216, 1421, 1882, 2797
HpyCH4IV ACGT 4 cut(s) 811, 1009, 1218, 1970
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 461, 470, 1331, 1340, 1587, 2768
HpyF3I CTNAG 9 cut(s) 30, 54, 468, 741, 1077, 1206, 1785, 2076, 2757
HpySE526I ACGT 4 cut(s) 811, 1009, 1218, 1970
HspAI GCGC 1 cut(s) 2815
Ksp22I TGATCA 1 cut(s) 2110
KspI CCGCGG 1 cut(s) 1166
LmnI GCTCC 3 cut(s) 434, 701, 2405
Lsp1109I GCAGC 7 cut(s) 108, 348, 448, 523, 1568, 1844, 2869
LweI GCATC 7 cut(s) 755, 1129, 1751, 1901, 2088, 2524, 3098
MaeII ACGT 4 cut(s) 811, 1009, 1218, 1970
MaeIII GTNAC 8 cut(s) 212, 554, 956, 1072, 1640, 1941, 2344, 3057
MfeI CAATTG 1 cut(s) 2136
MflI RGATCY 1 cut(s) 3105
MhlI GDGCHC 3 cut(s) 92, 1266, 2724
MlyI GAGTC 1 cut(s) 987
MmeI TCCRAC 3 cut(s) 206, 380, 770
Mph1103I ATGCAT 1 cut(s) 1894
MroXI GAANNNNTTC 3 cut(s) 445, 522, 1149
MslI CAYNNNNRTG 4 cut(s) 69, 954, 1656, 1982
MspA1I CMGCKG 1 cut(s) 1165
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 6 cut(s) 134, 756, 1578, 1862, 2277, 2930
MspR9I CCNGG 5 cut(s) 134, 280, 819, 1862, 2583
MunI CAATTG 1 cut(s) 2136
Mva1269I GAATGC 5 cut(s) 342, 1135, 2051, 2860, 3083
MvaI CCWGG 3 cut(s) 280, 819, 2583
MvnI CGCG 1 cut(s) 1165
MwoI GCNNNNNNNGC 7 cut(s) 96, 461, 470, 1331, 1340, 1587, 2768
NciI CCSGG 2 cut(s) 134, 1862
NcoI CCATGG 1 cut(s) 869
NlaIV GGNNCC 8 cut(s) 131, 284, 436, 491, 621, 1224, 1845, 2823
NmeAIII GCCGAG 1 cut(s) 2495
NmuCI GTSAC 6 cut(s) 956, 1072, 1640, 1941, 2344, 3057
NsiI ATGCAT 1 cut(s) 1894
NspI RCATGY 8 cut(s) 147, 326, 536, 914, 1343, 1622, 1810, 3119
NspV TTCGAA 1 cut(s) 2868
OliI CACNNNNGTG 1 cut(s) 954
PaeI GCATGC 2 cut(s) 147, 1810
PceI AGGCCT 1 cut(s) 151
PciI ACATGT 1 cut(s) 3115
PctI GAATGC 5 cut(s) 342, 1135, 2051, 2860, 3083
PdmI GAANNNNTTC 3 cut(s) 445, 522, 1149
PfeI GAWTC 8 cut(s) 26, 242, 793, 1821, 2059, 2377, 2806, 3014
PflMI CCANNNNNTGG 1 cut(s) 997
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 8 cut(s) 98, 363, 463, 538, 1193, 1583, 1859, 2859
PleI GAGTC 1 cut(s) 986
PpsI GAGTC 1 cut(s) 986
PscI ACATGT 1 cut(s) 3115
PshBI ATTAAT 1 cut(s) 2715
Psp124BI GAGCTC 1 cut(s) 1266
Psp6I CCWGG 3 cut(s) 278, 817, 2581
PspEI GGTNACC 1 cut(s) 956
PspFI CCCAGC 2 cut(s) 2537, 2619
PspGI CCWGG 3 cut(s) 278, 817, 2581
PspN4I GGNNCC 8 cut(s) 131, 284, 436, 491, 621, 1224, 1845, 2823
PspPI GGNCC 6 cut(s) 282, 919, 1222, 1750, 2549, 2821
PstI CTGCAG 3 cut(s) 25, 1927, 3053
PsuI RGATCY 1 cut(s) 3105
RsaI GTAC 8 cut(s) 1012, 1113, 1338, 1768, 1969, 2631, 2988, 3031
RsaNI GTAC 8 cut(s) 1011, 1112, 1337, 1767, 1968, 2630, 2987, 3030
RseI CAYNNNNRTG 4 cut(s) 69, 954, 1656, 1982
SacI GAGCTC 1 cut(s) 1266
SacII CCGCGG 1 cut(s) 1166
SatI GCNGC 8 cut(s) 97, 362, 462, 537, 1192, 1582, 1858, 2858
Sau96I GGNCC 6 cut(s) 282, 919, 1222, 1750, 2549, 2821
ScaI AGTACT 1 cut(s) 2988
SchI GAGTC 1 cut(s) 987
ScrFI CCNGG 5 cut(s) 134, 280, 819, 1862, 2583
SduI GDGCHC 3 cut(s) 92, 1266, 2724
SfaNI GCATC 7 cut(s) 755, 1129, 1751, 1901, 2088, 2524, 3098
SfcI CTRYAG 6 cut(s) 21, 1089, 1267, 1923, 2633, 3049
Sfr303I CCGCGG 1 cut(s) 1166
SfuI TTCGAA 1 cut(s) 2868
SgrBI CCGCGG 1 cut(s) 1166
SinI GGWCC 5 cut(s) 282, 919, 1750, 2549, 2821
SmiMI CAYNNNNRTG 4 cut(s) 69, 954, 1656, 1982
SmlI CTYRAG 3 cut(s) 83, 299, 424
SmoI CTYRAG 3 cut(s) 83, 299, 424
SphI GCATGC 2 cut(s) 147, 1810
SseBI AGGCCT 1 cut(s) 151
SsiI CCGC 6 cut(s) 229, 1163, 1165, 1191, 2682, 2951
SstI GAGCTC 1 cut(s) 1266
StuI AGGCCT 1 cut(s) 151
StyD4I CCNGG 5 cut(s) 132, 278, 817, 1860, 2581
StyI CCWWGG 3 cut(s) 869, 1746, 1838
TaiI ACGT 4 cut(s) 814, 1012, 1221, 1973
TaqI TCGA 8 cut(s) 109, 639, 1041, 1123, 2532, 2724, 2868, 3099
TatI WGTACW 3 cut(s) 1336, 2986, 3029
TauI GCSGC 1 cut(s) 1194
TfiI GAWTC 8 cut(s) 26, 242, 793, 1821, 2059, 2377, 2806, 3014
TscAI CASTG 7 cut(s) 240, 834, 956, 1218, 1276, 1283, 2120
TseFI GTSAC 6 cut(s) 956, 1072, 1640, 1941, 2344, 3057
TseI GCWGC 7 cut(s) 96, 361, 461, 536, 1581, 1857, 2857
Tsp45I GTSAC 6 cut(s) 956, 1072, 1640, 1941, 2344, 3057
TspGWI ACGGA 4 cut(s) 427, 1770, 2374, 2677
TspRI CASTG 7 cut(s) 240, 834, 956, 1218, 1276, 1283, 2120
Van91I CCANNNNNTGG 1 cut(s) 997
Vha464I CTTAAG 1 cut(s) 299
VpaK11BI GGWCC 5 cut(s) 282, 919, 1750, 2549, 2821
VspI ATTAAT 1 cut(s) 2715
XapI RAATTY 2 cut(s) 329, 1145
XceI RCATGY 8 cut(s) 147, 326, 536, 914, 1343, 1622, 1810, 3119
XmiI GTMKAC 1 cut(s) 1420
XmnI GAANNNNTTC 3 cut(s) 445, 522, 1149
ZrmI AGTACT 1 cut(s) 2988
Zsp2I ATGCAT 1 cut(s) 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.