Rorug05G0041900

protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000231
Physical Location & Seq
Forward (+)
3588581 .. 3595065
6485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0041900.1

Sequence Viewer

Length: 3141 bp
ATGGACCCTCTGGCCGGACCGGTGCCGGAGCATATGCGGTGCACGAGGACGGACGGCCGGCAATGGCGGTGCAAGAGGAGAGTGATGGAGAACATGAAGCTCTGCGAGATTCACTACCTGCAGGGACGGCACCGGCAGTACAGGGAGAAGGTGCCGGAGGAGCTGAAGATTCAGAGGAAGACGCAGACGGCGCCGTGCGAGGATCGAGACGGCGGCGGAGAAGGCGGAGGAATTAGGGCAAAGAAAGTGGACGATTTGGTGACGCTGCTGAAGAGGAAGAGGGCGGCGGAGGAGGCGGCGCCGGTGAAGATAAAGAAGGCGAAGGTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAGAGGGGGGGAGAAGAAGTGTGAGTTGAATTTGGAGCTGATAAGGATGGTGCTGAAGAGAGAGGTGGAGAAGAGGAACACGCCGCAGAAGAAGAAGAAGAAGATGAGGGTTTTGGTGGAGGAGGAGGGGAGTGAGGGTGAGGAGGGCCTTGATCTGACCAGAGACTTGCCTAATGGAAGAATGGCCATTTCGTCTTCTTCGTCGCAGTCTTTGGGGGGCAATGTAGGTTCCAATTCGGGCTCTGATGTTAGGGTTGGGGCCGAATTAGGGCCCCCCGTGCCCACTACTACCCGGCGGTGCTTTCGGTCCAAGAACATCGAGCCAATGCCTGTTGGCACAGTGCAGGTTTTGCCATATGGTGATGAGAATGAGGGGAGATTGAGGAAGGGTAAGAGGAAGAGGTGCCATTGGTGTCAGAGAAGTGGAAGTAGTGCTTCTTCCAGCTTGATTAAGTGTTCGAGTTGTAAGAAGCACTTGTTTTGCTTGAGCTGCATCAAAGAAAGGTATTTTGATACTCAAGAAGAAGTCAAAATGGCATGTCCAGTTTGTCGAGGAACTTGCCCCTGTAAGGAATGCTCTGAAAATCAACCGAAAGATGCTGAAAGTAAGGATTATGTGGGGGTTAAAAATAAAGTTGAAATTATATTACACTTTCACTACCTCGTCTGTATGCTTCTCCCTGTGCTGAAACAAATCAACCAAGATCAGAAATTTGAGCTGGAAGCAGAGGCAAAAATGAGAGGGAAAAAGCTCTCCGAAGTTTATATCAAGCAGGCTGAATTTAGCTGCAGTGAGCAAAACAGCTGCAATAAATGCAAAGCTCCCATTGTGGATCTTCACAGAAGCTGCCAAAATTGTTCCTATAACCTTTGTTTGAGCTGTTGTCGAGATCATTTTCAGGGGACTTTATGGGGAGGTATAAATACATTTCTCTCAAAGTGCTCTAATATAAATGATACTTGGGTCTCTGGTAAGGGGAAAGTTCAGAAAAGGCCAATAGCTACCCATAAGCAAAATGTCCGGTACCTGTCGTCATTTGCATCAACTAACTGGAATGCTTGTAATGCTGTTGACGGTATATCTTGCCCGCCCAAGGAGTTTGGTGGTTGTGGTGACAGCTTCCTTGATTTGAGATGTTTTTTCCCTTTAAGTTGGATCAAAGAGCTAGAAGTAAGTGCAGAAGAAATAGTTTGCAGCTATGAGTTTCCAGAAACTTCAGACATGTCATTGAGCTGCCCTCTATGTTTGGACACGGACCAGAATGTCGATGGACTTCAACAGTTGCAAGAAACTGCTTTGAGAGAAAATTCAAATGATAACTATCTATTCTACCCCACGCTTTCAGACGTACATGGTGATAATGTTGAACATTTTCAGAAGCACTGGAGTAAAGGTCATCCTGTTATAGTCCGAGATGTCCTTCGGAGTACGTCAGATTTGAGTTGGGATCCAGTATTCATGTTCTGCACTTATCTTGAGAGGAGCATTGCTAGATATGAAAATAACAAGAATCCACAGGAAGCTATCCACTGCTTGGATTGGTGTGAGGTGGAAATGGGGATCAGGCAGTATTTTATGGGTTCATTGAACGGGCGAACACAAAAAGACATGTGGAGTGAGACCCTGAAATTGAAGGGTTGGCTTTCTTCACAATTATTTCAAGAACAGTTTCCAGCTCATTATGCTGAAATCATCCAAGCTCTACCACTTCAAGAGTACATGAATCCCTCGTCTGGTCTTTTAAATGTTGCTGCAAGGATGCCACAGGAAATCCCAAAACCTGATCTAGGTCCATGTGTTTATATTTCATATGGGTGCAGTGAGCAGTTTGTACAGGCTGATTCAGTGATAAAGTTGTGTTATGACTCATATGATGTGGTTAACATTTTGGCACATACATCAGATGCCCCTATTTCTGATGAACAAGTTTCTAAGATAAGAAAGTTACTGAAAAGGCACAATCAAAATCAGAGGGAGTCTTCTAGGAATACTTCTGAACAAACTAGAGCAAAAAAAGTTAATGGAAATTCAGCATTGCATAGTCAAAACAAGGAACAAGTGAGGTCACATAATGTGATTGGAGAAGAGATGCATTTACGGAAAAGAATTGCAAGAGAATCTTGCTTCTCTGCTGCAACGCGTGAAGAATGCACTAGGAATCTCAAAGAGAGTAATATGTCACTTGATGGGGAATCTGATGTTGATTCTGAGGCCACGTTGAGTTGCTCTGGGTCTATCAATGGTGATGAAACATCTGAAGATGAGGAGTCACCTGATCTCCTTGAAAGCTCAAATGGCCATGAAAAACCCTTTGTAGAGTCATGCACTGCACAGTGGGATGTTTTCCGCCGGCAAGATGTTCCGAAACTTGTAGAATATCTCAGAAGGCACTGTAATGAATTTACTCGTAAATATGACGTTCATAAACATGTTGCTCATCCAATTCTTGATCAGAGTTTCTTTCTAGACTCGATTCACAAAATAAGACTCAAGGAGGAATTTAAAATTGAACCTTGGACTTTTGAGCAACACATTGGAGAAGCTGTCATCATCCCTGCTGGATGCCCGTACCAGATTAGTAGTCCTAAGTCTTGTGTACATGTGGTGCTCGATTTTATGTCTCCCGAAAATGTTACCGAGTGCATCCAGTTGACAGATGAAGTTCGCCTGCTTCCAGAAGATCATAAAGCAAAAGTGGACAAGCTGGAGGTGAAGAGAATGGCACTCTATAGTATTAGTTCAGCAATCAAAGAAATACGTGAGCTTACTTGTGCAATTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

1046

Amino Acids

119.09

Weight (kDa)

8.29

Isoelectric Point (pI)

59.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 9 - 50 2.8e-16 WRC
JmjC PF02373 893 - 991 3.8e-11 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000231)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr0c21g0500361 RchiOBHm_Chr0c31g0501281 RchiOBHm_Chr0c31g0501361 RchiOBHm_Chr1g0366571 RchiOBHm_Chr1g0368811 RchiOBHm_Chr1g0382111 RchiOBHm_Chr2g0121351 RchiOBHm_Chr2g0121651 RchiOBHm_Chr2g0121971 RchiOBHm_Chr2g0122291 RchiOBHm_Chr2g0122411 RchiOBHm_Chr2g0124731 RchiOBHm_Chr2g0124741 RchiOBHm_Chr2g0125141 RchiOBHm_Chr2g0125201 RchiOBHm_Chr2g0125231 RchiOBHm_Chr2g0125321 RchiOBHm_Chr2g0125571 RchiOBHm_Chr2g0125691 RchiOBHm_Chr2g0125971 RchiOBHm_Chr2g0126271 RchiOBHm_Chr2g0126441 RchiOBHm_Chr2g0127451 RchiOBHm_Chr2g0128391 RchiOBHm_Chr2g0128871 RchiOBHm_Chr2g0129451 RchiOBHm_Chr2g0129741 RchiOBHm_Chr2g0129961 RchiOBHm_Chr2g0131161 RchiOBHm_Chr2g0131871 RchiOBHm_Chr2g0132661 RchiOBHm_Chr2g0134171 RchiOBHm_Chr2g0136421 RchiOBHm_Chr2g0137241 RchiOBHm_Chr2g0138031 RchiOBHm_Chr2g0139031 RchiOBHm_Chr2g0139331 RchiOBHm_Chr2g0139341 RchiOBHm_Chr2g0139441 RchiOBHm_Chr2g0141451 RchiOBHm_Chr2g0146701 RchiOBHm_Chr2g0147781 RchiOBHm_Chr2g0148841 RchiOBHm_Chr2g0148961 RchiOBHm_Chr2g0150261 RchiOBHm_Chr2g0152651 RchiOBHm_Chr2g0155711 RchiOBHm_Chr2g0156101 RchiOBHm_Chr2g0156321 RchiOBHm_Chr2g0157801 RchiOBHm_Chr2g0158491 RchiOBHm_Chr2g0161671 RchiOBHm_Chr2g0164891 RchiOBHm_Chr2g0165241 RchiOBHm_Chr2g0165281 RchiOBHm_Chr2g0165811 RchiOBHm_Chr2g0166781 RchiOBHm_Chr2g0168521 RchiOBHm_Chr2g0170181 RchiOBHm_Chr2g0171501 RchiOBHm_Chr2g0171931 RchiOBHm_Chr2g0172151 RchiOBHm_Chr2g0175071 RchiOBHm_Chr2g0176251 RchiOBHm_Chr2g0176731 RchiOBHm_Chr3g0447841 RchiOBHm_Chr3g0453271 RchiOBHm_Chr3g0456381 RchiOBHm_Chr3g0458661 RchiOBHm_Chr3g0458791 RchiOBHm_Chr3g0461201 RchiOBHm_Chr3g0462241 RchiOBHm_Chr3g0464391 RchiOBHm_Chr3g0465591 RchiOBHm_Chr3g0469851 RchiOBHm_Chr3g0470331 RchiOBHm_Chr4g0402011 RchiOBHm_Chr4g0403391 RchiOBHm_Chr4g0436281 RchiOBHm_Chr6g0247471 RchiOBHm_Chr6g0273091 RchiOBHm_Chr7g0180431 RchiOBHm_Chr7g0182691 RchiOBHm_Chr7g0190741 RchiOBHm_Chr7g0193021 RchiOBHm_Chr7g0193851 RchiOBHm_Chr7g0194191 RchiOBHm_Chr7g0195951 RchiOBHm_Chr7g0196551 RchiOBHm_Chr7g0196831 RchiOBHm_Chr7g0198051 RchiOBHm_Chr7g0198071 RchiOBHm_Chr7g0198081 RchiOBHm_Chr7g0199681
rosa_multiflora Rmu_sc0004153.1_g000005 Rmu_sc0005666.1_g000001
rosa_rugosa Rorug01G0001400 Rorug01G0001400 Rorug01G0077900 Rorug01G0082600 Rorug01G0082600 Rorug01G0096200 Rorug01G0096300 Rorug01G0261400 Rorug01G0448400 Rorug02G0114600 Rorug02G0185400 Rorug02G0201900 Rorug02G0253700 Rorug02G0257600 Rorug02G0282800 Rorug02G0298500 Rorug03G0113800 Rorug03G0158300 Rorug03G0215200 Rorug03G0216900 Rorug03G0265300 Rorug03G0288500 Rorug03G0359900.1 Rorug04G0065400 Rorug04G0066200 Rorug04G0069300 Rorug04G0253100 Rorug04G0361900 Rorug04G0419800 Rorug05G0041900 Rorug05G0147900 Rorug05G0257500 Rorug05G0259100 Rorug05G0304100 Rorug05G0340600 Rorug05G0460500 Rorug05G0583400 Rorug06G0050700 Rorug06G0056300 Rorug06G0065300 Rorug06G0083000 Rorug06G0171200 Rorug06G0451100 Rorug07G0168200 Rorug07G0169100 Rorug07G0197000 Rorug07G0302300 RorugPtG0001500.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1619
Acc36I ACCTGC 2 cut(s) 126, 691
Acc65I GGTACC 1 cut(s) 1380
AccB1I GGYRCC 7 cut(s) 22, 129, 151, 190, 298, 759, 1380
AccB7I CCANNNNNTGG 1 cut(s) 1891
AccII CGCG 1 cut(s) 2497
AclWI GGATC 6 cut(s) 210, 1197, 1520, 1799, 1812, 1925
AcoI YGGCCR 4 cut(s) 12, 55, 540, 2653
AcsI RAATTY 7 cut(s) 385, 1067, 1136, 1663, 2383, 2756, 2855
AcuI CTGAAG 5 cut(s) 185, 290, 431, 1557, 2634
AcyI GRCGYC 2 cut(s) 191, 299
AfaI GTAC 8 cut(s) 140, 1382, 1707, 1786, 2075, 2190, 2927, 2955
AfiI CCNNNNNNNGG 7 cut(s) 14, 624, 925, 1450, 1891, 2090, 2144
AflIII ACRYGT 5 cut(s) 1578, 1965, 2495, 2785, 2956
AgeI ACCGGT 1 cut(s) 19
Alw21I GWGCWC 3 cut(s) 44, 1301, 2967
Alw26I GTCTC 5 cut(s) 201, 513, 1327, 1970, 2982
Alw44I GTGCAC 1 cut(s) 40
AlwI GGATC 6 cut(s) 210, 1197, 1520, 1799, 1812, 1925
AlwNI CAGNNNCTG 1 cut(s) 1203
AoxI GGCC 9 cut(s) 12, 55, 502, 540, 615, 626, 1349, 2568, 2653
ApaI GGGCCC 1 cut(s) 630
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 9 cut(s) 265, 846, 1143, 1161, 1203, 1551, 1590, 2108, 2489
ApoI RAATTY 7 cut(s) 385, 1067, 1136, 1663, 2383, 2756, 2855
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 2 cut(s) 1728, 2025
Asp718I GGTACC 1 cut(s) 1380
AspLEI GCGC 2 cut(s) 193, 301
AspS9I GGNCC 9 cut(s) 4, 17, 502, 615, 626, 627, 663, 1612, 2147
AsuC2I CCSGG 1 cut(s) 649
AvaII GGWCC 5 cut(s) 4, 17, 663, 1612, 2147
BaeGI GKGCMC 3 cut(s) 44, 630, 639
BaeI ACNNNNGTAYC 2 cut(s) 1305, 1338
BalI TGGCCA 2 cut(s) 542, 2655
BamHI GGATCC 1 cut(s) 1804
BanI GGYRCC 7 cut(s) 22, 129, 151, 190, 298, 759, 1380
BanII GRGCYC 2 cut(s) 599, 630
BarI GAAGNNNNNNTAC 2 cut(s) 778, 810
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 3 cut(s) 185, 543, 2328
Bbv12I GWGCWC 3 cut(s) 44, 1301, 2967
BbvI GCAGC 9 cut(s) 252, 833, 1130, 1148, 1190, 1563, 1577, 2095, 2476
BccI CCATC 4 cut(s) 79, 397, 1619, 2537
BceAI ACGGC 5 cut(s) 70, 143, 178, 204, 226
BcgI CGANNNNNNTGC 2 cut(s) 897, 931
BclI TGATCA 1 cut(s) 2806
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 5 cut(s) 201, 513, 1327, 1970, 2982
BfaI CTAG 7 cut(s) 1523, 1848, 2144, 2340, 2361, 2511, 2822
BfmI CTRYAG 3 cut(s) 119, 1144, 3085
BfoI RGCGCY 2 cut(s) 194, 302
BfuAI ACCTGC 2 cut(s) 126, 691
Bme1390I CCNGG 1 cut(s) 649
Bme18I GGWCC 5 cut(s) 4, 17, 663, 1612, 2147
BmgT120I GGNCC 9 cut(s) 4, 17, 502, 615, 626, 627, 663, 1612, 2147
BmrFI CCNGG 1 cut(s) 649
BmsI GCATC 8 cut(s) 858, 943, 1406, 2106, 2251, 2436, 2909, 3009
BpiI GAAGAC 3 cut(s) 185, 543, 2328
BplI GAGNNNNNCTC 2 cut(s) 1579, 1611
BpmI CTGGAG 2 cut(s) 1762, 3083
BpuEI CTTGAG 4 cut(s) 858, 862, 1853, 2831
BpuMI CCSGG 1 cut(s) 649
BsaAI YACGTR 1 cut(s) 3116
BsaBI GATNNNNATC 1 cut(s) 1677
BsaHI GRCGYC 2 cut(s) 191, 299
BsaI GGTCTC 2 cut(s) 1327, 1970
BsaJI CCNNGG 2 cut(s) 1449, 2870
BsaWI WCCGGW 2 cut(s) 19, 1377
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 624, 925, 1450, 1891, 2090, 2144
Bse118I RCCGGY 5 cut(s) 19, 57, 132, 301, 2706
Bse1I ACTGG 5 cut(s) 899, 1412, 1745, 1808, 3004
Bse3DI GCAATG 4 cut(s) 68, 583, 1842, 2390
Bse8I GATNNNNATC 1 cut(s) 1677
BseDI CCNNGG 2 cut(s) 1449, 2870
BseGI GGATG 9 cut(s) 408, 1753, 2049, 2121, 2701, 2794, 2907, 2924, 3000
BseJI GATNNNNATC 1 cut(s) 1677
BseLI CCNNNNNNNGG 7 cut(s) 14, 624, 925, 1450, 1891, 2090, 2144
BseMI GCAATG 4 cut(s) 68, 583, 1842, 2390
BseMII CTCAG 2 cut(s) 2556, 2752
BseNI ACTGG 5 cut(s) 899, 1412, 1745, 1808, 3004
BseRI GAGGAG 9 cut(s) 91, 173, 305, 370, 491, 494, 512, 1852, 2636
BseSI GKGCMC 3 cut(s) 44, 630, 639
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 9 cut(s) 252, 833, 1130, 1148, 1190, 1563, 1577, 2095, 2476
BsgI GTGCAG 5 cut(s) 719, 1554, 1807, 2194, 2670
Bsh1236I CGCG 1 cut(s) 2497
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 9 cut(s) 14, 57, 504, 542, 617, 628, 1351, 2570, 2655
BshNI GGYRCC 7 cut(s) 22, 129, 151, 190, 298, 759, 1380
BshTI ACCGGT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 44, 1301, 2967
BslFI GGGAC 2 cut(s) 138, 1273
BslI CCNNNNNNNGG 7 cut(s) 14, 624, 925, 1450, 1891, 2090, 2144
BsmAI GTCTC 5 cut(s) 201, 513, 1327, 1970, 2982
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 138, 1273
BsmI GAATGC 3 cut(s) 935, 1417, 2510
BsnI GGCC 9 cut(s) 14, 57, 504, 542, 617, 628, 1351, 2570, 2655
Bso31I GGTCTC 2 cut(s) 1327, 1970
Bsp120I GGGCCC 1 cut(s) 626
Bsp1286I GDGCHC 6 cut(s) 44, 599, 630, 639, 1301, 2967
Bsp1407I TGTACA 2 cut(s) 2188, 2953
BspANI GGCC 9 cut(s) 14, 57, 504, 542, 617, 628, 1351, 2570, 2655
BspCNI CTCAG 2 cut(s) 2557, 2751
BspFNI CGCG 1 cut(s) 2497
BspMAI CTGCAG 2 cut(s) 123, 1148
BspMI ACCTGC 2 cut(s) 126, 691
BspPI GGATC 6 cut(s) 210, 1197, 1520, 1799, 1812, 1925
BspT107I GGYRCC 7 cut(s) 22, 129, 151, 190, 298, 759, 1380
BspTNI GGTCTC 2 cut(s) 1327, 1970
BsrDI GCAATG 4 cut(s) 68, 583, 1842, 2390
BsrFI RCCGGY 5 cut(s) 19, 57, 132, 301, 2706
BsrGI TGTACA 2 cut(s) 2188, 2953
BsrI ACTGG 5 cut(s) 899, 1412, 1745, 1808, 3004
BssAI RCCGGY 5 cut(s) 19, 57, 132, 301, 2706
BssECI CCNNGG 2 cut(s) 1449, 2870
BssNI GRCGYC 2 cut(s) 191, 299
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 2 cut(s) 1449, 2870
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 6 cut(s) 697, 1433, 1638, 2025, 2691, 2750
Bst6I CTCTTC 8 cut(s) 266, 272, 347, 407, 422, 749, 2436, 3065
BstACI GRCGYC 2 cut(s) 191, 299
BstAPI GCANNNNNTGC 2 cut(s) 706, 1170
BstAUI TGTACA 2 cut(s) 2188, 2953
BstBAI YACGTR 1 cut(s) 3116
BstC8I GCNNGC 5 cut(s) 59, 1131, 1445, 2708, 3026
BstDEI CTNAG 4 cut(s) 2289, 2565, 2738, 2943
BstENI CCTNNNNNAGG 1 cut(s) 2142
BstF5I GGATG 9 cut(s) 408, 1753, 2049, 2121, 2701, 2794, 2907, 2924, 3000
BstFNI CGCG 1 cut(s) 2497
BstH2I RGCGCY 2 cut(s) 194, 302
BstHHI GCGC 2 cut(s) 193, 301
BstMAI GTCTC 5 cut(s) 201, 513, 1327, 1970, 2982
BstMCI CGRYCG 1 cut(s) 58
BstNSI RCATGY 5 cut(s) 897, 1582, 1969, 2789, 2960
BstSCI CCNGG 1 cut(s) 647
BstSFI CTRYAG 3 cut(s) 119, 1144, 3085
BstSLI GKGCMC 3 cut(s) 44, 630, 639
BstUI CGCG 1 cut(s) 2497
BstV1I GCAGC 9 cut(s) 252, 833, 1130, 1148, 1190, 1563, 1577, 2095, 2476
BstV2I GAAGAC 3 cut(s) 185, 543, 2328
BstX2I RGATCY 2 cut(s) 1189, 1804
BstYI RGATCY 2 cut(s) 1189, 1804
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 9 cut(s) 14, 57, 504, 542, 617, 628, 1351, 2570, 2655
BtsCI GGATG 9 cut(s) 408, 1753, 2049, 2121, 2701, 2794, 2907, 2924, 3000
BtsI GCAGTG 4 cut(s) 1153, 1885, 2182, 2682
BtsIMutI CAGTG 9 cut(s) 702, 1153, 1738, 1885, 2182, 2208, 2682, 2696, 2746
BveI ACCTGC 2 cut(s) 126, 691
Cac8I GCNNGC 5 cut(s) 59, 1131, 1445, 2708, 3026
CaiI CAGNNNCTG 1 cut(s) 1203
CfoI GCGC 2 cut(s) 193, 301
Cfr10I RCCGGY 5 cut(s) 19, 57, 132, 301, 2706
Cfr13I GGNCC 9 cut(s) 4, 17, 502, 615, 626, 627, 663, 1612, 2147
CpoI CGGWCCG 1 cut(s) 17
CseI GACGC 2 cut(s) 190, 271
Csp6I GTAC 8 cut(s) 139, 1381, 1706, 1785, 2074, 2189, 2926, 2954
CspAI ACCGGT 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 228, 263
CspI CGGWCCG 1 cut(s) 17
CviQI GTAC 8 cut(s) 139, 1381, 1706, 1785, 2074, 2189, 2926, 2954
DdeI CTNAG 4 cut(s) 2289, 2565, 2738, 2943
DinI GGCGCC 2 cut(s) 192, 300
DraI TTTAAA 2 cut(s) 2100, 2860
DrdI GACNNNNNNGTC 1 cut(s) 1619
DseDI GACNNNNNNGTC 1 cut(s) 1619
EaeI YGGCCR 4 cut(s) 12, 55, 540, 2653
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 8 cut(s) 266, 272, 347, 407, 422, 749, 2436, 3065
EarI CTCTTC 8 cut(s) 266, 272, 347, 407, 422, 749, 2436, 3065
EciI GGCGGA 4 cut(s) 231, 240, 302, 2693
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 1449, 2870
Eco24I GRGCYC 2 cut(s) 599, 630
Eco31I GGTCTC 2 cut(s) 1327, 1970
Eco47I GGWCC 5 cut(s) 4, 17, 663, 1612, 2147
Eco52I CGGCCG 1 cut(s) 55
Eco57I CTGAAG 5 cut(s) 185, 290, 431, 1557, 2634
EcoNI CCTNNNNNAGG 1 cut(s) 2142
EcoO109I RGGNCCY 3 cut(s) 502, 626, 627
EcoT14I CCWWGG 2 cut(s) 1449, 2870
EcoT22I ATGCAT 1 cut(s) 2451
EcoT38I GRGCYC 2 cut(s) 599, 630
EgeI GGCGCC 2 cut(s) 192, 300
EheI GGCGCC 2 cut(s) 192, 300
ErhI CCWWGG 2 cut(s) 1449, 2870
Esp3I CGTCTC 1 cut(s) 201
FalI AAGNNNNNCTT 6 cut(s) 775, 807, 815, 847, 2461, 2493
FaqI GGGAC 2 cut(s) 138, 1273
FauI CCCGC 1 cut(s) 1452
FauNDI CATATG 4 cut(s) 33, 712, 2167, 2227
FbaI TGATCA 1 cut(s) 2806
FokI GGATG 9 cut(s) 415, 1740, 2036, 2128, 2708, 2781, 2894, 2931, 2987
FriOI GRGCYC 2 cut(s) 599, 630
FspBI CTAG 7 cut(s) 1523, 1848, 2144, 2340, 2361, 2511, 2822
GlaI GCGC 2 cut(s) 192, 300
GsuI CTGGAG 2 cut(s) 1762, 3083
HaeII RGCGCY 2 cut(s) 194, 302
HaeIII GGCC 9 cut(s) 14, 57, 504, 542, 617, 628, 1351, 2570, 2655
HgaI GACGC 2 cut(s) 190, 271
HhaI GCGC 2 cut(s) 193, 301
Hin1I GRCGYC 2 cut(s) 191, 299
Hin6I GCGC 2 cut(s) 191, 299
HinP1I GCGC 2 cut(s) 191, 299
HincII GTYRAC 3 cut(s) 1429, 2239, 3009
HindII GTYRAC 3 cut(s) 1429, 2239, 3009
HpaI GTTAAC 1 cut(s) 2239
Hpy166II GTNNAC 7 cut(s) 42, 250, 1429, 2239, 2954, 3009, 3055
Hpy8I GTNNAC 7 cut(s) 42, 250, 1429, 2239, 2954, 3009, 3055
Hpy99I CGWCG 1 cut(s) 562
HpyAV CCTTC 8 cut(s) 142, 215, 310, 316, 736, 1787, 1984, 2736
HpyCH4III ACNGT 6 cut(s) 697, 1433, 1638, 2025, 2691, 2750
HpyCH4IV ACGT 5 cut(s) 1704, 1787, 2573, 2775, 3115
HpyF3I CTNAG 4 cut(s) 2289, 2565, 2738, 2943
HpySE526I ACGT 5 cut(s) 1704, 1787, 2573, 2775, 3115
Hsp92I GRCGYC 2 cut(s) 191, 299
HspAI GCGC 2 cut(s) 191, 299
KasI GGCGCC 2 cut(s) 190, 298
KpnI GGTACC 1 cut(s) 1384
KroI GCCGGC 2 cut(s) 57, 2706
KroNI GCCGGC 2 cut(s) 59, 2708
Ksp22I TGATCA 1 cut(s) 2806
KspAI GTTAAC 1 cut(s) 2239
LmnI GCTCC 5 cut(s) 28, 160, 391, 1183, 1839
Lsp1109I GCAGC 9 cut(s) 252, 833, 1130, 1148, 1190, 1563, 1577, 2095, 2476
LweI GCATC 8 cut(s) 858, 943, 1406, 2106, 2251, 2436, 2909, 3009
MaeI CTAG 7 cut(s) 1523, 1848, 2144, 2340, 2361, 2511, 2822
MaeII ACGT 5 cut(s) 1704, 1787, 2573, 2775, 3115
MaeIII GTNAC 7 cut(s) 259, 1469, 2301, 2421, 2535, 2625, 2989
MfeI CAATTG 1 cut(s) 3132
MflI RGATCY 2 cut(s) 1189, 1804
MhlI GDGCHC 6 cut(s) 44, 599, 630, 639, 1301, 2967
MlsI TGGCCA 2 cut(s) 542, 2655
MluI ACGCGT 1 cut(s) 2495
MluNI TGGCCA 2 cut(s) 542, 2655
Mly113I GGCGCC 2 cut(s) 191, 299
MlyI GAGTC 6 cut(s) 2216, 2342, 2633, 2684, 2819, 2838
MmeI TCCRAC 1 cut(s) 1490
Mox20I TGGCCA 2 cut(s) 542, 2655
Mph1103I ATGCAT 1 cut(s) 2451
MroNI GCCGGC 2 cut(s) 57, 2706
MroXI GAANNNNTTC 2 cut(s) 1728, 2025
MscI TGGCCA 2 cut(s) 542, 2655
MseI TTAA 7 cut(s) 807, 981, 1505, 2099, 2238, 2376, 2859
MslI CAYNNNNRTG 3 cut(s) 2170, 2751, 2784
Msp20I TGGCCA 2 cut(s) 542, 2655
MspA1I CMGCKG 1 cut(s) 1161
MspR9I CCNGG 1 cut(s) 649
MunI CAATTG 1 cut(s) 3132
Mva1269I GAATGC 3 cut(s) 935, 1417, 2510
MvnI CGCG 1 cut(s) 2497
NaeI GCCGGC 2 cut(s) 59, 2708
NarI GGCGCC 2 cut(s) 191, 299
NciI CCSGG 1 cut(s) 649
NdeI CATATG 4 cut(s) 33, 712, 2167, 2227
NgoMIV GCCGGC 2 cut(s) 57, 2706
NmuCI GTSAC 5 cut(s) 259, 1469, 2421, 2535, 2625
NsiI ATGCAT 1 cut(s) 2451
NspI RCATGY 5 cut(s) 897, 1582, 1969, 2789, 2960
PciI ACATGT 4 cut(s) 1578, 1965, 2785, 2956
PcsI WCGNNNNNNNCGW 1 cut(s) 554
PctI GAATGC 3 cut(s) 935, 1417, 2510
PdiI GCCGGC 2 cut(s) 59, 2708
PdmI GAANNNNTTC 2 cut(s) 1728, 2025
PflMI CCANNNNNTGG 1 cut(s) 1891
PinAI ACCGGT 1 cut(s) 19
PleI GAGTC 6 cut(s) 2216, 2341, 2632, 2683, 2819, 2838
PluTI GGCGCC 2 cut(s) 194, 302
PpsI GAGTC 6 cut(s) 2216, 2341, 2632, 2683, 2819, 2838
Ppu21I YACGTR 1 cut(s) 3116
PscI ACATGT 4 cut(s) 1578, 1965, 2785, 2956
PspOMI GGGCCC 1 cut(s) 626
PspPI GGNCC 9 cut(s) 4, 17, 502, 615, 626, 627, 663, 1612, 2147
PstI CTGCAG 2 cut(s) 123, 1148
PstNI CAGNNNCTG 1 cut(s) 1203
PsuI RGATCY 2 cut(s) 1189, 1804
PvuII CAGCTG 1 cut(s) 1161
RsaI GTAC 8 cut(s) 140, 1382, 1707, 1786, 2075, 2190, 2927, 2955
RsaNI GTAC 8 cut(s) 139, 1381, 1706, 1785, 2074, 2189, 2926, 2954
RseI CAYNNNNRTG 3 cut(s) 2170, 2751, 2784
Rsr2I CGGWCCG 1 cut(s) 17
RsrII CGGWCCG 1 cut(s) 17
SaqAI TTAA 7 cut(s) 807, 981, 1505, 2099, 2238, 2376, 2859
Sau96I GGNCC 9 cut(s) 4, 17, 502, 615, 626, 627, 663, 1612, 2147
SbfI CCTGCAGG 1 cut(s) 123
SchI GAGTC 6 cut(s) 2216, 2342, 2633, 2684, 2819, 2838
ScrFI CCNGG 1 cut(s) 649
SdaI CCTGCAGG 1 cut(s) 123
SduI GDGCHC 6 cut(s) 44, 599, 630, 639, 1301, 2967
SfaNI GCATC 8 cut(s) 858, 943, 1406, 2106, 2251, 2436, 2909, 3009
SfcI CTRYAG 3 cut(s) 119, 1144, 3085
SfoI GGCGCC 2 cut(s) 192, 300
SgrAI CRCCGGYG 1 cut(s) 301
SinI GGWCC 5 cut(s) 4, 17, 663, 1612, 2147
SmiMI CAYNNNNRTG 3 cut(s) 2170, 2751, 2784
SmlI CTYRAG 4 cut(s) 841, 873, 1832, 2846
SmoI CTYRAG 4 cut(s) 841, 873, 1832, 2846
Sse8387I CCTGCAGG 1 cut(s) 123
SspDI GGCGCC 2 cut(s) 190, 298
SspMI CTAG 7 cut(s) 1523, 1848, 2144, 2340, 2361, 2511, 2822
StyD4I CCNGG 1 cut(s) 647
StyI CCWWGG 2 cut(s) 1449, 2870
TaaI ACNGT 6 cut(s) 697, 1433, 1638, 2025, 2691, 2750
TaiI ACGT 5 cut(s) 1707, 1790, 2576, 2778, 3118
TaqI TCGA 8 cut(s) 205, 675, 815, 907, 1243, 1623, 2828, 2967
TaqII GACCGA 1 cut(s) 651
TatI WGTACW 4 cut(s) 138, 2073, 2188, 2953
TauI GCSGC 4 cut(s) 216, 287, 299, 442
Tru1I TTAA 7 cut(s) 807, 981, 1505, 2099, 2238, 2376, 2859
Tru9I TTAA 7 cut(s) 807, 981, 1505, 2099, 2238, 2376, 2859
TscAI CASTG 9 cut(s) 702, 1153, 1745, 1892, 2182, 2208, 2689, 2696, 2753
TseFI GTSAC 5 cut(s) 259, 1469, 2421, 2535, 2625
TseI GCWGC 9 cut(s) 265, 846, 1143, 1161, 1203, 1551, 1590, 2108, 2489
Tsp45I GTSAC 5 cut(s) 259, 1469, 2421, 2535, 2625
TspGWI ACGGA 3 cut(s) 65, 1625, 2470
TspRI CASTG 9 cut(s) 702, 1153, 1745, 1892, 2182, 2208, 2689, 2696, 2753
Van91I CCANNNNNTGG 1 cut(s) 1891
VneI GTGCAC 1 cut(s) 40
VpaK11BI GGWCC 5 cut(s) 4, 17, 663, 1612, 2147
XagI CCTNNNNNAGG 1 cut(s) 2142
XapI RAATTY 7 cut(s) 385, 1067, 1136, 1663, 2383, 2756, 2855
XbaI TCTAGA 1 cut(s) 2821
XceI RCATGY 5 cut(s) 897, 1582, 1969, 2789, 2960
XcmI CCANNNNNNNNNTGG 1 cut(s) 1622
XmnI GAANNNNTTC 2 cut(s) 1728, 2025
XspI CTAG 7 cut(s) 1523, 1848, 2144, 2340, 2361, 2511, 2822
Zsp2I ATGCAT 1 cut(s) 2451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.