Rorug07G0197000

Protein timeless homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000231
Physical Location & Seq
Forward (+)
17014578 .. 17023756
9179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0197000.1

Sequence Viewer

Length: 2034 bp
ATGCGAGTGAAGGAGATGCACCCGCTCTGCTGTATAACGCTGGAGAGCGCCGGCATCGGCGACCAGTCGCCGGAGGCGTCGTTGCTGTCGAGGACGCGGAGCTTGCCGGCGAGTCTGGCGACGGCTTCGCTCGGATCTGCCGGCAATGCCGGACGGCAGACGGGATCTGAGGCCAGCGTCGCTGGAGTTCTCCACAAGTGGACCAATTACGGCAAGGGATGGAGATCCAGGTGGTTCCTGCTGCGAAACGGCGTCCTCTCCTACGCCAAGATTCGGCTGCCGGAGAATCTCAACCTCCTCAACCCAAACGACGACGTTCGCGTCATCGGCGAGATCTCGACTCGGACCACGCACCGGCTTTCGAGGATTGACAGCGGGAGAATCGGGCGACGGAAGCACCAGAAAACCGTTGGAATCGTCCACCTCAAGATTTCATCCTTCAGAGAAAGCAAGTCGGATGATCGGAAGTTTTACATATTTACTGCTACAAAGACTCTTCATTTAAGGACTAATTCAAAGAGAGACCGGGCGGCTTGGTTGCAAGCTTTGGCGTCAACCCGGAGTCTATTTCCACTGAGATCGTTGAATGATAATCTCTCCCTTGTACAAACGGATTTATCTATATCTACTGAGAGACTAAAGAGCCGCCTACTTGAAGAGGGAACCAGTGAGAACACTGTAAAGGATTGTGAGCAGATAATGCTCTCTGAATTCTCTCAATTACAAGGACAGCTCAAGGTTCTGTGCGAAGAACGGTCCAATTTGTTCGACACATTAAGACAGTTGGAGGCAGCTAACTTTGAAGCTGAAGCCTCTGGAATTCACGATGGTGAATACCAATTGACGAAGCATGAGTTTTCCAGTTTGCGGCAGGGAAAATATAGTGAATGCAGCACAACTGAATCATCTGACGATATTGAGAAACAGGAGCTCGAGGAAGTGTCAGATGAAGATGAATTGTCATTTTTTGACACAAAAGAGTATTTTACTGAACCGAACATAAGTTGCGGGTCTGTGCAAGTTGTAAATAACACAAATAAGATCAACAAACCTCGAAGTCAATTTAGTAATGTTGAGGAGATGCACACAGAAAAGGAAATTAATGATGTTAGATATCCACACATTGAAAGGAGAAAAGAGCTTCCTGTTCCAGTTGAGAAGGAGAAAGGAGTCAGCCTTTGGTCCATGATTAAAGACAATGTGGGAAAGGATCTCACTCGAGTTTGTCTCCCTGTGTATTTTAATGAGCCAATATCATCTCTTCAGAAGTGTTGTGAGGACATGGAGTACTCGTACCTTTTGGATCGGGCATATGAGTATGGAAAAATGGGGAATAGTCTCCAAAGGATTCTAAATGTTGCTGCATTTGCGGTTTCTGGTTATGCATCATCGGAAGGGCGGCACTGTAAGCCATTCAATCCTTTGTTAGGTGAAACTTATGAAGCCGACTATCCAGATAAAGGAATTCACTTCTTCTCTGAAAAGGTCAGTCACCACCCAACACTCATTGCTTGCCACTGTGAAGGTAGAGGGTGGAAGTTCTGGGCTGACAGTAATCTCCGGTCAAAATTTTGGGGGCGATCAATTCAGCTTGACCCTGTTGGAGTTCTAAGCGTGGAGTTTGATGATGGCGAAATTTTCCAGTGGAGCAAGGTCACAACAAATATATATAATCTCATTCTCGGTAAACTTTATTGTGACCACCATGGTACAATGCACATACGGGGTAATCGTGAGTATTCATGCAAACTGAAGTTCAAAGAGCAATCCATTCTTGACCGAAATCCTCACCAGGTCCATGGATTTGTTGAAGATGTCGTGGGGAAAAAGGTTGCTACACTATTTGGGAAGTGGGATGACAGCATGTATTATGTTAATGGTGATGTGGGTGGAAAGCCAAATCCTTCAGATGCTTCCTTATTGTGGAAAGGAAGTAAACCCCCTAATATCACACGGTACAACTTAACATCGTTCGCTATCACACTGAACGAGCTAACACCTGGACTGCAGCTTTGTCTGATCCTGGGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIMELESS TIMELESS_C

Protein Analysis

677

Amino Acids

76.71

Weight (kDa)

6.86

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 62 - 185 3e-08 PH domain
PH_11 PF15413 66 - 184 2.6e-10 Pleckstrin homology domain
Oxysterol_BP PF01237 392 - 667 3.5e-96 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000231)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr0c21g0500361 RchiOBHm_Chr0c31g0501281 RchiOBHm_Chr0c31g0501361 RchiOBHm_Chr1g0366571 RchiOBHm_Chr1g0368811 RchiOBHm_Chr1g0382111 RchiOBHm_Chr2g0121351 RchiOBHm_Chr2g0121651 RchiOBHm_Chr2g0121971 RchiOBHm_Chr2g0122291 RchiOBHm_Chr2g0122411 RchiOBHm_Chr2g0124731 RchiOBHm_Chr2g0124741 RchiOBHm_Chr2g0125141 RchiOBHm_Chr2g0125201 RchiOBHm_Chr2g0125231 RchiOBHm_Chr2g0125321 RchiOBHm_Chr2g0125571 RchiOBHm_Chr2g0125691 RchiOBHm_Chr2g0125971 RchiOBHm_Chr2g0126271 RchiOBHm_Chr2g0126441 RchiOBHm_Chr2g0127451 RchiOBHm_Chr2g0128391 RchiOBHm_Chr2g0128871 RchiOBHm_Chr2g0129451 RchiOBHm_Chr2g0129741 RchiOBHm_Chr2g0129961 RchiOBHm_Chr2g0131161 RchiOBHm_Chr2g0131871 RchiOBHm_Chr2g0132661 RchiOBHm_Chr2g0134171 RchiOBHm_Chr2g0136421 RchiOBHm_Chr2g0137241 RchiOBHm_Chr2g0138031 RchiOBHm_Chr2g0139031 RchiOBHm_Chr2g0139331 RchiOBHm_Chr2g0139341 RchiOBHm_Chr2g0139441 RchiOBHm_Chr2g0141451 RchiOBHm_Chr2g0146701 RchiOBHm_Chr2g0147781 RchiOBHm_Chr2g0148841 RchiOBHm_Chr2g0148961 RchiOBHm_Chr2g0150261 RchiOBHm_Chr2g0152651 RchiOBHm_Chr2g0155711 RchiOBHm_Chr2g0156101 RchiOBHm_Chr2g0156321 RchiOBHm_Chr2g0157801 RchiOBHm_Chr2g0158491 RchiOBHm_Chr2g0161671 RchiOBHm_Chr2g0164891 RchiOBHm_Chr2g0165241 RchiOBHm_Chr2g0165281 RchiOBHm_Chr2g0165811 RchiOBHm_Chr2g0166781 RchiOBHm_Chr2g0168521 RchiOBHm_Chr2g0170181 RchiOBHm_Chr2g0171501 RchiOBHm_Chr2g0171931 RchiOBHm_Chr2g0172151 RchiOBHm_Chr2g0175071 RchiOBHm_Chr2g0176251 RchiOBHm_Chr2g0176731 RchiOBHm_Chr3g0447841 RchiOBHm_Chr3g0453271 RchiOBHm_Chr3g0456381 RchiOBHm_Chr3g0458661 RchiOBHm_Chr3g0458791 RchiOBHm_Chr3g0461201 RchiOBHm_Chr3g0462241 RchiOBHm_Chr3g0464391 RchiOBHm_Chr3g0465591 RchiOBHm_Chr3g0469851 RchiOBHm_Chr3g0470331 RchiOBHm_Chr4g0402011 RchiOBHm_Chr4g0403391 RchiOBHm_Chr4g0436281 RchiOBHm_Chr6g0247471 RchiOBHm_Chr6g0273091 RchiOBHm_Chr7g0180431 RchiOBHm_Chr7g0182691 RchiOBHm_Chr7g0190741 RchiOBHm_Chr7g0193021 RchiOBHm_Chr7g0193851 RchiOBHm_Chr7g0194191 RchiOBHm_Chr7g0195951 RchiOBHm_Chr7g0196551 RchiOBHm_Chr7g0196831 RchiOBHm_Chr7g0198051 RchiOBHm_Chr7g0198071 RchiOBHm_Chr7g0198081 RchiOBHm_Chr7g0199681
rosa_multiflora Rmu_sc0004153.1_g000005 Rmu_sc0005666.1_g000001
rosa_rugosa Rorug01G0001400 Rorug01G0001400 Rorug01G0077900 Rorug01G0082600 Rorug01G0082600 Rorug01G0096200 Rorug01G0096300 Rorug01G0261400 Rorug01G0448400 Rorug02G0114600 Rorug02G0185400 Rorug02G0201900 Rorug02G0253700 Rorug02G0257600 Rorug02G0282800 Rorug02G0298500 Rorug03G0113800 Rorug03G0158300 Rorug03G0215200 Rorug03G0216900 Rorug03G0265300 Rorug03G0288500 Rorug03G0359900.1 Rorug04G0065400 Rorug04G0066200 Rorug04G0069300 Rorug04G0253100 Rorug04G0361900 Rorug04G0419800 Rorug05G0041900 Rorug05G0147900 Rorug05G0257500 Rorug05G0259100 Rorug05G0304100 Rorug05G0340600 Rorug05G0460500 Rorug05G0583400 Rorug06G0050700 Rorug06G0056300 Rorug06G0065300 Rorug06G0083000 Rorug06G0171200 Rorug06G0451100 Rorug07G0168200 Rorug07G0169100 Rorug07G0197000 Rorug07G0302300 RorugPtG0001500.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AccBSI CCGCTC 1 cut(s) 25
AccII CGCG 2 cut(s) 97, 321
AciI CCGC 9 cut(s) 23, 97, 375, 530, 646, 868, 1008, 1370, 1399
AclWI GGATC 6 cut(s) 142, 172, 219, 1218, 1311, 2014
AcsI RAATTY 5 cut(s) 710, 819, 1464, 1568, 1635
AcuI CTGAAG 5 cut(s) 424, 828, 1247, 1772, 1890
AcyI GRCGYC 3 cut(s) 77, 252, 551
AfaI GTAC 5 cut(s) 606, 1289, 1295, 1711, 1958
AfiI CCNNNNNNNGG 7 cut(s) 70, 273, 354, 867, 1427, 1460, 1923
AgsI TTSAA 8 cut(s) 516, 586, 656, 803, 1127, 1417, 1759, 1811
AjnI CCWGG 4 cut(s) 227, 1791, 1999, 2022
AleI CACNNNNGTG 1 cut(s) 828
Alw21I GWGCWC 1 cut(s) 933
Alw26I GTCTC 4 cut(s) 516, 628, 1232, 1343
AlwI GGATC 6 cut(s) 142, 172, 219, 1218, 1311, 2014
Ama87I CYCGRG 2 cut(s) 932, 1218
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 6 cut(s) 241, 277, 791, 891, 1361, 2008
ApoI RAATTY 5 cut(s) 710, 819, 1464, 1568, 1635
ArsI GACNNNNNNTTYG 2 cut(s) 1995, 2027
AseI ATTAAT 1 cut(s) 1101
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 5 cut(s) 201, 345, 756, 1182, 1795
AsuC2I CCSGG 2 cut(s) 527, 559
AsuHPI GGTGA 5 cut(s) 842, 1442, 1484, 1781, 1892
AvaI CYCGRG 2 cut(s) 932, 1218
AvaII GGWCC 5 cut(s) 201, 345, 756, 1182, 1795
BanII GRGCYC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 933
BbvI GCAGC 6 cut(s) 228, 264, 803, 903, 1348, 2020
BccI CCATC 3 cut(s) 213, 821, 1622
BceAI ACGGC 4 cut(s) 138, 170, 226, 265
BciT130I CCWGG 4 cut(s) 229, 1793, 2001, 2024
BcnI CCSGG 2 cut(s) 527, 559
BcoDI GTCTC 4 cut(s) 516, 628, 1232, 1343
BfmI CTRYAG 1 cut(s) 2006
BfoI RGCGCY 1 cut(s) 51
BglII AGATCT 1 cut(s) 333
BmcAI AGTACT 1 cut(s) 1289
Bme1390I CCNGG 6 cut(s) 229, 527, 559, 1793, 2001, 2024
Bme18I GGWCC 5 cut(s) 201, 345, 756, 1182, 1795
BmeT110I CYCGRG 2 cut(s) 932, 1218
BmgT120I GGNCC 5 cut(s) 201, 345, 756, 1182, 1795
BmiI GGNNCC 2 cut(s) 236, 664
BmrFI CCNGG 6 cut(s) 229, 527, 559, 1793, 2001, 2024
BmsI GCATC 5 cut(s) 6, 63, 1071, 1394, 1900
BplI GAGNNNNNCTC 2 cut(s) 1212, 1244
BpmI CTGGAG 2 cut(s) 62, 204
BpuEI CTTGAG 2 cut(s) 410, 719
BpuMI CCSGG 2 cut(s) 527, 559
BsaBI GATNNNNATC 1 cut(s) 223
BsaHI GRCGYC 3 cut(s) 77, 252, 551
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 1705, 1798, 2023
BsaWI WCCGGW 1 cut(s) 1560
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 1277, 1307
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 273, 354, 867, 1427, 1460, 1923
Bse118I RCCGGY 4 cut(s) 50, 106, 140, 354
Bse1I ACTGG 5 cut(s) 64, 666, 861, 1151, 1642
Bse3DI GCAATG 2 cut(s) 151, 1506
Bse8I GATNNNNATC 1 cut(s) 223
BseBI CCWGG 4 cut(s) 229, 1793, 2001, 2024
BseDI CCNNGG 3 cut(s) 1705, 1798, 2023
BseGI GGATG 4 cut(s) 224, 434, 463, 1861
BseJI GATNNNNATC 1 cut(s) 223
BseLI CCNNNNNNNGG 7 cut(s) 70, 273, 354, 867, 1427, 1460, 1923
BseMI GCAATG 2 cut(s) 151, 1506
BseMII CTCAG 3 cut(s) 159, 566, 621
BseNI ACTGG 5 cut(s) 64, 666, 861, 1151, 1642
BseRI GAGGAG 2 cut(s) 287, 1091
BseXI GCAGC 6 cut(s) 228, 264, 803, 903, 1348, 2020
Bsh1236I CGCG 2 cut(s) 97, 321
BshFI GGCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 933
BsiHKCI CYCGRG 2 cut(s) 932, 1218
BslI CCNNNNNNNGG 7 cut(s) 70, 273, 354, 867, 1427, 1460, 1923
BsmAI GTCTC 4 cut(s) 516, 628, 1232, 1343
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 1 cut(s) 173
Bso31I GGTCTC 1 cut(s) 516
BsoBI CYCGRG 2 cut(s) 932, 1218
Bsp1286I GDGCHC 1 cut(s) 933
Bsp1407I TGTACA 1 cut(s) 604
Bsp19I CCATGG 2 cut(s) 1705, 1798
BspACI CCGC 9 cut(s) 23, 97, 375, 530, 646, 868, 1008, 1370, 1399
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 3 cut(s) 160, 567, 622
BspFNI CGCG 2 cut(s) 97, 321
BspLI GGNNCC 2 cut(s) 236, 664
BspMAI CTGCAG 1 cut(s) 2010
BspPI GGATC 6 cut(s) 142, 172, 219, 1218, 1311, 2014
BspTNI GGTCTC 1 cut(s) 516
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 2 cut(s) 151, 1506
BsrFI RCCGGY 4 cut(s) 50, 106, 140, 354
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 5 cut(s) 64, 666, 861, 1151, 1642
BssAI RCCGGY 4 cut(s) 50, 106, 140, 354
BssECI CCNNGG 3 cut(s) 1705, 1798, 2023
BssNI GRCGYC 3 cut(s) 77, 252, 551
BssT1I CCWWGG 2 cut(s) 1705, 1798
Bst2UI CCWGG 4 cut(s) 229, 1793, 2001, 2024
Bst4CI ACNGT 8 cut(s) 409, 679, 756, 783, 1406, 1520, 1553, 1956
Bst6I CTCTTC 3 cut(s) 501, 651, 1266
BstACI GRCGYC 3 cut(s) 77, 252, 551
BstAPI GCANNNNNTGC 1 cut(s) 700
BstAUI TGTACA 1 cut(s) 604
BstC8I GCNNGC 7 cut(s) 52, 104, 108, 142, 175, 543, 1513
BstDEI CTNAG 4 cut(s) 168, 575, 630, 1610
BstDSI CCRYGG 2 cut(s) 1705, 1798
BstENI CCTNNNNNAGG 1 cut(s) 1425
BstF5I GGATG 4 cut(s) 224, 434, 463, 1861
BstFNI CGCG 2 cut(s) 97, 321
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstMAI GTCTC 4 cut(s) 516, 628, 1232, 1343
BstMWI GCNNNNNNNGC 9 cut(s) 103, 116, 146, 179, 327, 394, 700, 1367, 1408
BstNI CCWGG 4 cut(s) 229, 1793, 2001, 2024
BstNSI RCATGY 1 cut(s) 1867
BstSCI CCNGG 6 cut(s) 227, 525, 557, 1791, 1999, 2022
BstSFI CTRYAG 1 cut(s) 2006
BstUI CGCG 2 cut(s) 97, 321
BstV1I GCAGC 6 cut(s) 228, 264, 803, 903, 1348, 2020
BstX2I RGATCY 5 cut(s) 134, 164, 224, 333, 1210
BstXI CCANNNNNNTGG 1 cut(s) 1799
BstYI RGATCY 5 cut(s) 134, 164, 224, 333, 1210
BsuRI GGCC 1 cut(s) 173
BtgI CCRYGG 2 cut(s) 1705, 1798
BtsCI GGATG 4 cut(s) 224, 434, 463, 1861
BtsIMutI CAGTG 7 cut(s) 572, 673, 675, 1402, 1516, 1649, 1982
Cac8I GCNNGC 7 cut(s) 52, 104, 108, 142, 175, 543, 1513
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 4 cut(s) 50, 106, 140, 354
Cfr13I GGNCC 5 cut(s) 201, 345, 756, 1182, 1795
CseI GACGC 6 cut(s) 66, 103, 166, 241, 310, 540
CsiI ACCWGGT 1 cut(s) 1791
Csp6I GTAC 5 cut(s) 605, 1288, 1294, 1710, 1957
CviAII CATG 7 cut(s) 851, 1186, 1282, 1706, 1743, 1799, 1864
CviQI GTAC 5 cut(s) 605, 1288, 1294, 1710, 1957
DdeI CTNAG 4 cut(s) 168, 575, 630, 1610
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
Eam1104I CTCTTC 3 cut(s) 501, 651, 1266
EarI CTCTTC 3 cut(s) 501, 651, 1266
Ecl136II GAGCTC 1 cut(s) 931
Eco130I CCWWGG 2 cut(s) 1705, 1798
Eco24I GRGCYC 1 cut(s) 933
Eco31I GGTCTC 1 cut(s) 516
Eco32I GATATC 1 cut(s) 1115
Eco47I GGWCC 5 cut(s) 201, 345, 756, 1182, 1795
Eco53kI GAGCTC 1 cut(s) 931
Eco57I CTGAAG 5 cut(s) 424, 828, 1247, 1772, 1890
Eco88I CYCGRG 2 cut(s) 932, 1218
EcoICRI GAGCTC 1 cut(s) 931
EcoNI CCTNNNNNAGG 1 cut(s) 1425
EcoRI GAATTC 3 cut(s) 710, 819, 1464
EcoRII CCWGG 4 cut(s) 227, 1791, 1999, 2022
EcoRV GATATC 1 cut(s) 1115
EcoT14I CCWWGG 2 cut(s) 1705, 1798
EcoT22I ATGCAT 1 cut(s) 1387
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 2 cut(s) 1705, 1798
FaeI CATG 7 cut(s) 854, 1189, 1285, 1709, 1746, 1802, 1867
FatI CATG 7 cut(s) 850, 1185, 1281, 1705, 1742, 1798, 1863
FauI CCCGC 3 cut(s) 30, 368, 1001
FauNDI CATATG 1 cut(s) 1312
FokI GGATG 4 cut(s) 231, 421, 470, 1868
FriOI GRGCYC 1 cut(s) 933
GlaI GCGC 1 cut(s) 49
GsuI CTGGAG 2 cut(s) 62, 204
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 173
HgaI GACGC 6 cut(s) 66, 103, 166, 241, 310, 540
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 3 cut(s) 77, 252, 551
Hin1II CATG 7 cut(s) 854, 1189, 1285, 1709, 1746, 1802, 1867
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HindIII AAGCTT 1 cut(s) 543
HphI GGTGA 5 cut(s) 842, 1442, 1484, 1781, 1892
Hpy166II GTNNAC 5 cut(s) 201, 421, 555, 1688, 1937
Hpy188III TCNNGA 7 cut(s) 337, 427, 816, 824, 1454, 1733, 1775
Hpy8I GTNNAC 5 cut(s) 201, 421, 555, 1688, 1937
Hpy99I CGWCG 6 cut(s) 82, 124, 182, 314, 317, 393
HpyAV CCTTC 6 cut(s) 4, 448, 1153, 1388, 1517, 1914
HpyCH4III ACNGT 8 cut(s) 409, 679, 756, 783, 1406, 1520, 1553, 1956
HpyCH4IV ACGT 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 9 cut(s) 103, 116, 146, 179, 327, 394, 700, 1367, 1408
HpyF3I CTNAG 4 cut(s) 168, 575, 630, 1610
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 3 cut(s) 77, 252, 551
Hsp92II CATG 7 cut(s) 854, 1189, 1285, 1709, 1746, 1802, 1867
HspAI GCGC 1 cut(s) 48
KroI GCCGGC 3 cut(s) 50, 106, 140
KroNI GCCGGC 3 cut(s) 52, 108, 142
LmnI GCTCC 3 cut(s) 99, 928, 1647
Lsp1109I GCAGC 6 cut(s) 228, 264, 803, 903, 1348, 2020
LweI GCATC 5 cut(s) 6, 63, 1071, 1394, 1900
MabI ACCWGGT 1 cut(s) 1791
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 3 cut(s) 1490, 1654, 1697
MbiI CCGCTC 1 cut(s) 25
MboII GAAGA 7 cut(s) 488, 668, 761, 962, 1253, 1465, 1823
MfeI CAATTG 1 cut(s) 839
MflI RGATCY 5 cut(s) 134, 164, 224, 333, 1210
MhlI GDGCHC 1 cut(s) 933
MlyI GAGTC 5 cut(s) 121, 334, 487, 571, 1179
MmeI TCCRAC 4 cut(s) 391, 435, 765, 1582
Mph1103I ATGCAT 1 cut(s) 1387
MroNI GCCGGC 3 cut(s) 50, 106, 140
MseI TTAA 7 cut(s) 503, 776, 1101, 1191, 1242, 1875, 1964
MslI CAYNNNNRTG 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 375
MspR9I CCNGG 6 cut(s) 229, 527, 559, 1793, 2001, 2024
MunI CAATTG 1 cut(s) 839
Mva1269I GAATGC 1 cut(s) 893
MvaI CCWGG 4 cut(s) 229, 1793, 2001, 2024
MvnI CGCG 2 cut(s) 97, 321
MwoI GCNNNNNNNGC 9 cut(s) 103, 116, 146, 179, 327, 394, 700, 1367, 1408
NaeI GCCGGC 3 cut(s) 52, 108, 142
NciI CCSGG 2 cut(s) 527, 559
NcoI CCATGG 2 cut(s) 1705, 1798
NdeI CATATG 1 cut(s) 1312
NgoMIV GCCGGC 3 cut(s) 50, 106, 140
NlaIII CATG 7 cut(s) 854, 1189, 1285, 1709, 1746, 1802, 1867
NlaIV GGNNCC 2 cut(s) 236, 664
NmuCI GTSAC 3 cut(s) 1490, 1654, 1697
NsiI ATGCAT 1 cut(s) 1387
NspI RCATGY 1 cut(s) 1867
OliI CACNNNNGTG 1 cut(s) 828
PaeR7I CTCGAG 2 cut(s) 932, 1218
PcsI WCGNNNNNNNCGW 4 cut(s) 74, 86, 318, 1729
PctI GAATGC 1 cut(s) 893
PdiI GCCGGC 3 cut(s) 52, 108, 142
PfeI GAWTC 6 cut(s) 271, 286, 381, 414, 902, 1348
PleI GAGTC 5 cut(s) 120, 334, 487, 570, 1178
PpsI GAGTC 5 cut(s) 120, 334, 487, 570, 1178
PshBI ATTAAT 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 933
Psp6I CCWGG 4 cut(s) 227, 1791, 1999, 2022
PspGI CCWGG 4 cut(s) 227, 1791, 1999, 2022
PspN4I GGNNCC 2 cut(s) 236, 664
PspPI GGNCC 5 cut(s) 201, 345, 756, 1182, 1795
PspXI VCTCGAGB 2 cut(s) 932, 1218
PstI CTGCAG 1 cut(s) 2010
PsuI RGATCY 5 cut(s) 134, 164, 224, 333, 1210
RsaI GTAC 5 cut(s) 606, 1289, 1295, 1711, 1958
RsaNI GTAC 5 cut(s) 605, 1288, 1294, 1710, 1957
RseI CAYNNNNRTG 1 cut(s) 828
SacI GAGCTC 1 cut(s) 933
SaqAI TTAA 7 cut(s) 503, 776, 1101, 1191, 1242, 1875, 1964
Sau96I GGNCC 5 cut(s) 201, 345, 756, 1182, 1795
ScaI AGTACT 1 cut(s) 1289
SchI GAGTC 5 cut(s) 121, 334, 487, 571, 1179
ScrFI CCNGG 6 cut(s) 229, 527, 559, 1793, 2001, 2024
SduI GDGCHC 1 cut(s) 933
SexAI ACCWGGT 1 cut(s) 1791
SfaNI GCATC 5 cut(s) 6, 63, 1071, 1394, 1900
SfcI CTRYAG 1 cut(s) 2006
Sfr274I CTCGAG 2 cut(s) 932, 1218
SinI GGWCC 5 cut(s) 201, 345, 756, 1182, 1795
SlaI CTCGAG 2 cut(s) 932, 1218
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 4 cut(s) 425, 734, 932, 1218
SmoI CTYRAG 4 cut(s) 425, 734, 932, 1218
SsiI CCGC 9 cut(s) 23, 97, 375, 530, 646, 868, 1008, 1370, 1399
SstI GAGCTC 1 cut(s) 933
StyD4I CCNGG 6 cut(s) 227, 525, 557, 1791, 1999, 2022
StyI CCWWGG 2 cut(s) 1705, 1798
TaaI ACNGT 8 cut(s) 409, 679, 756, 783, 1406, 1520, 1553, 1956
TaiI ACGT 1 cut(s) 318
TaqI TCGA 7 cut(s) 89, 338, 362, 768, 933, 1054, 1219
TaqII GACCGA 1 cut(s) 1794
TatI WGTACW 2 cut(s) 604, 1287
TauI GCSGC 4 cut(s) 533, 648, 871, 1402
TfiI GAWTC 6 cut(s) 271, 286, 381, 414, 902, 1348
Tru1I TTAA 7 cut(s) 503, 776, 1101, 1191, 1242, 1875, 1964
Tru9I TTAA 7 cut(s) 503, 776, 1101, 1191, 1242, 1875, 1964
TscAI CASTG 7 cut(s) 579, 673, 682, 1409, 1523, 1649, 1989
TseFI GTSAC 3 cut(s) 1490, 1654, 1697
TseI GCWGC 6 cut(s) 241, 277, 791, 891, 1361, 2008
Tsp45I GTSAC 3 cut(s) 1490, 1654, 1697
TspDTI ATGAA 6 cut(s) 423, 488, 963, 969, 1455, 1731
TspGWI ACGGA 2 cut(s) 406, 626
TspRI CASTG 7 cut(s) 579, 673, 682, 1409, 1523, 1649, 1989
VpaK11BI GGWCC 5 cut(s) 201, 345, 756, 1182, 1795
VspI ATTAAT 1 cut(s) 1101
XagI CCTNNNNNAGG 1 cut(s) 1425
XapI RAATTY 5 cut(s) 710, 819, 1464, 1568, 1635
XceI RCATGY 1 cut(s) 1867
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XhoI CTCGAG 2 cut(s) 932, 1218
ZrmI AGTACT 1 cut(s) 1289
Zsp2I ATGCAT 1 cut(s) 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.