Rh5BG133900

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003891
Physical Location & Seq
Forward (+)
13306568 .. 13312406
5839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG133900.1

Sequence Viewer

Length: 3009 bp
ATGGAAGGGACGAGTTTTGCAGCAGAATCTCAGGCAAAAGAGGTTCATTCTTCTAACAACAACCAGAGAAGTGATAACACAGTTGAGTGCTCTGGCAGTCCTCTAAGTCGAAAGATATCAGCTAGATGGGATCCAGATGAAGCATGCCGGCCTGTCATTGATGATGCACCTGTATTTTATCCAACTGCCGAGGAGTTCGAAAATACACTTGGTTACATAGCAAAGATTTGGCCACAGGCTGAATCTTATGGTATTTGTCGGATTGTCCCTCCACGTTCCTGGATCCCTCCTTGCCCTCTTAAAGAGAAAGACATGTGGGAAAATGCTAAATTCTCTACTCGTGTTCAGCAAGTTGACTTGCTTCAAAATAGAGAGTCCATGAAAAAGAAAACTAGAGGTCGGAAGCGAAAACGAAGAAGACACTCAAGGAGACGTGCAGAAGCTAATGCTGCTTCTGAGACCGATGAGAAGTTTGGGTTCCAGTCAGGATCAGACTTTACATTTGCAGAGTTTCAGAGACAAGCCTCTACTTTCAAGGAAAGTTACTTTGGAACACATGAATGTGAGGAAGGTTCAAATTCTGGTGGAACTAACAACAAAAGATGGGAACCCTCTGTGGAGGATATTGAAGGTGAATACTGGCGCATAGTAGAGCAACCAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGGTTTCCCAAGGCATCGTCTGTGGTAACTAGGAGTGAGTCAGATCATTATGCAATGTCAGGCTGGAATCTAAATAACTTGCCTCGCTTGCCTGGTTCTGTACTATGTTTTGAGGAAAGCGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCGTTTTGTTGGCATGTTGAGGACCACCACCTCTATTCCCTAAATTATCTACACTTCGGTGACCCAAAGGTATGGTATGGAGTGCCTGGAAGCCATGCTACCTCTTTGGAACAAGCAATGAGAAAGCATTTGCCGGATTTGTTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTTCAGTTTTAAAGTCTGAGGGTGTACCTGTGCATCGAGCTATCCAACATTCTGGGGAGTTTGTTCTTACCTTTCCGAGGGCATATCATTCTGGATTTAATTGTGGCTTCAATTGTGCTGAGGCAGTAAATGTCGCCCCTGTTGACTGGTTGCAACATGGCCAAACCGCAGTCGAGCTTTACAGTAAGCAGTGTCGCAAGACATCACTGTCACACGACAAATTGTTACTGGGGTCAGCTCTGGAAGCCGTGCAGGTCCTCGGGGAAATATCACTCGGAACAAAATTTATTACCAACCGGAGCTGGCAAAAAGTTTGTGGCAAAGATGGTGTGCTCACCAAAGCAGTTAAGAGACGAGTGGAGATGGAGGAGGAAAGACTGGATCGTCTTCCAATTTGTTGGAAGTCGCAAAAGATGGAAAGAGAATTTGATTCAAACACTGAGAGAGAATGCTTTTCTTGCTCCTATGACTTACACTTATCTGCTGCCAGCTGCAACTGCTCTCCTGACCGATTTTCATGTCTTAAACATGCAAAGCATTTCTGTTCATGTGAAATGACTCAGAGATATGTCCTTCTCCGCTACAGTATTGAGGAATTAAATTTGCTGGTTAAAGCATTGGAAGGGGAGTTGGATGCCATTCACGTATGGGCATCTAAGGATTCTGGAGTAATTTCTATAGACTACACTCGTAAGTGCGCTGCTAAGAAGCCTAAGCTGGATCAAGATTCCAAGTCTTGCGATCCAATGGAAATTTTGCCTGCTTGCGCAGTTTCAGAAGATAAGGTTAACATGAATGGATCTTGCAGTTCCTCTAGTCCTGTTTCTTCTGCAGTAGTTCAGTCAGGATCACAGGATGGTCATAATGAAGATCAAACTTTGGCCGTGAATGCTGAAGCAAAGGTGGAGCATGATTGCAGTTTTGATTTGAATCTCAATTGTATGTCTGATGAACATGAAAGCAAGATAATTGACGTATCTGACGGCTGTGATAATAAGGCTAGTACAGTTGAGGAAGAGACATCCACATCAATGTCCAATCAAGAGAAGACTAGCACGTCCGAGGGTAATAAGTTGTTTGGTGTTGATCTTGGCTTATCACGTCCTGCTTCAAACATACCACCAATCAGTTCTTCACAAACTGAGATTGGCGATACCTCAGCTGTGAATGCATCCTTGAGAGACCAAAGCTATCGATTAAGATGTTCGAGTGTTGTTGAGCCTTTGAATTTCGGATCTGTCGTGGCTGGGAAATATTGGTGCACTAAGCAGGCCATATATCCAAAAGGGTTTAGAAGCCGAGTTAATTTCTACAGTGTGCTGGACCCAACAAAACTGTGTAGTTATATATCAGAAGTTCTTGATGCTGGGCTTCTTGGACCCCTATTTAAGGTTAGTTTAGAAGAATTCCCAGATGAGGCTTTTGCAAATGTATCAGCAGACAAGTGCTGGGAGATGGTACTACATAGACTAAACAATGAAATAAGTAGACGGAGTAGTCTAGATGAAAGAGGGCCCCCTTTACAGTCTTTGCAGAGCATTAACGGGTTTGCAATGTTTGGCTTTCTCTCCCAACCTATTGTGGAGGCTATTGAGGCCCTTGATCCTGATCATCTATGCACAGAGTACTGGAATCACAGGTGTACGCAGCAGCATTCATCTGTACCAAGTTGCTCTCTAGGAGCGCCGCAGACAAAGCTATTTGGTATAAACATGACAAACAAGGAGCATAATCAACCATCTGTGGAGGGTCATCACTCAATCGATGAGATGCAGTTAGTTTTACGAAGACTCTTAGAGAAGGCGAATCCAAATCCCGAAGAGTTGAGGACATTACATAGAATGTTATCCAGTCAGTCAGCCGAAGCAAGAGTGGCATGCGCAGAATTGATTGAAGAGATGCAGAGAAATGTAGATAACAGGAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1002

Amino Acids

112.32

Weight (kDa)

5.46

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 57 - 90 2.2e-15 jmjN domain
JmjC PF02373 288 - 404 2.6e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 510 - 560 1.8e-14 C5HC2 zinc finger
FYRN PF05964 759 - 814 1.1e-12 F/Y-rich N-terminus
FYRC PF05965 819 - 906 6.2e-21 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2134, 2574
Acc16I TGCGCA 2 cut(s) 1846, 2959
Acc36I ACCTGC 1 cut(s) 1321
AccI GTMKAC 1 cut(s) 2566
AciI CCGC 3 cut(s) 1245, 1657, 2765
AcoI YGGCCR 3 cut(s) 230, 1237, 1959
AcsI RAATTY 8 cut(s) 329, 577, 1361, 1502, 1678, 1830, 2305, 2483
AcuI CTGAAG 2 cut(s) 1065, 1992
AfaI GTAC 7 cut(s) 819, 1104, 2083, 2538, 2705, 2722, 2742
AfiI CCNNNNNNNGG 3 cut(s) 1155, 2467, 2494
AflIII ACRYGT 1 cut(s) 312
AjiI CACGTC 3 cut(s) 434, 2136, 2180
AjnI CCWGG 3 cut(s) 278, 808, 973
AjuI GAANNNNNNNTTGG 6 cut(s) 192, 224, 531, 563, 2194, 2226
AleI CACNNNNGTG 1 cut(s) 945
Alw21I GWGCWC 3 cut(s) 92, 1413, 2342
Alw26I GTCTC 6 cut(s) 424, 452, 511, 1423, 2090, 2253
Alw44I GTGCAC 1 cut(s) 2338
Ama87I CYCGRG 2 cut(s) 844, 1337
AoxI GGCC 7 cut(s) 149, 230, 1237, 1959, 2349, 2591, 2673
ApaI GGGCCC 1 cut(s) 2595
ApaLI GTGCAC 1 cut(s) 2338
ApeKI GCWGC 7 cut(s) 20, 449, 1562, 1569, 1778, 2725, 2728
ApoI RAATTY 8 cut(s) 329, 577, 1361, 1502, 1678, 1830, 2305, 2483
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 5 cut(s) 645, 1778, 1847, 2764, 2960
AspS9I GGNCC 7 cut(s) 910, 1333, 2401, 2456, 2591, 2592, 2674
AsuHPI GGTGA 3 cut(s) 644, 959, 1405
AsuII TTCGAA 1 cut(s) 198
AvaI CYCGRG 2 cut(s) 844, 1337
AvaII GGWCC 4 cut(s) 910, 1333, 2401, 2456
BaeGI GKGCMC 2 cut(s) 2342, 2595
BalI TGGCCA 2 cut(s) 232, 1239
BamHI GGATCC 2 cut(s) 130, 282
BanII GRGCYC 1 cut(s) 2595
BarI GAAGNNNNNNTAC 2 cut(s) 970, 1002
BauI CACGAG 1 cut(s) 339
BbsI GAAGAC 4 cut(s) 424, 1457, 2132, 2872
Bbv12I GWGCWC 3 cut(s) 92, 1413, 2342
BbvCI CCTCAGC 2 cut(s) 1197, 2236
BbvI GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2737, 2740
BccI CCATC 8 cut(s) 120, 597, 1397, 1435, 1486, 1928, 2527, 2824
BceAI ACGGC 3 cut(s) 1310, 1946, 2077
BciT130I CCWGG 3 cut(s) 280, 810, 975
BclI TGATCA 1 cut(s) 2686
BcoDI GTCTC 6 cut(s) 424, 452, 511, 1423, 2090, 2253
BfaI CTAG 9 cut(s) 123, 393, 747, 854, 1893, 2079, 2130, 2579, 2756
BfmI CTRYAG 4 cut(s) 1660, 1755, 1908, 2389
BfoI RGCGCY 1 cut(s) 2765
BfuAI ACCTGC 1 cut(s) 1321
BisI GCNGC 8 cut(s) 21, 450, 1563, 1570, 1779, 2726, 2729, 2765
BlsI GCNGC 8 cut(s) 22, 451, 1564, 1571, 1780, 2727, 2730, 2766
BmcAI AGTACT 1 cut(s) 2705
Bme1390I CCNGG 3 cut(s) 280, 810, 975
Bme18I GGWCC 4 cut(s) 910, 1333, 2401, 2456
BmeT110I CYCGRG 2 cut(s) 844, 1337
BmgBI CACGTC 3 cut(s) 434, 2136, 2180
BmgT120I GGNCC 7 cut(s) 910, 1333, 2401, 2456, 2591, 2592, 2674
BmiI GGNNCC 8 cut(s) 132, 284, 479, 609, 2403, 2458, 2593, 2594
BmrFI CCNGG 3 cut(s) 280, 810, 975
BmrI ACTGGG 1 cut(s) 1316
BmsI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2258, 2431, 2838, 2967
BmuI ACTGGG 1 cut(s) 1316
BpiI GAAGAC 4 cut(s) 424, 1457, 2132, 2872
BpmI CTGGAG 1 cut(s) 1764
Bpu10I CCTNAGC 3 cut(s) 1197, 1791, 2236
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 409, 2275
Bsa29I ATCGAT 2 cut(s) 2272, 2841
BsaAI YACGTR 1 cut(s) 1723
BsaBI GATNNNNATC 2 cut(s) 2007, 2685
BsaI GGTCTC 2 cut(s) 452, 2253
BsaJI CCNNGG 6 cut(s) 189, 726, 860, 1154, 1336, 2139
BsaWI WCCGGW 1 cut(s) 1374
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 1155, 2467, 2494
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 8 cut(s) 481, 644, 1065, 1229, 1311, 1461, 2711, 2928
Bse3DI GCAATG 3 cut(s) 777, 1011, 2637
Bse8I GATNNNNATC 2 cut(s) 2007, 2685
BseBI CCWGG 3 cut(s) 280, 810, 975
BseCI ATCGAT 2 cut(s) 2272, 2841
BseDI CCNNGG 6 cut(s) 189, 726, 860, 1154, 1336, 2139
BseGI GGATG 5 cut(s) 882, 1717, 1939, 2099, 2249
BseJI GATNNNNATC 2 cut(s) 2007, 2685
BseLI CCNNNNNNNGG 3 cut(s) 1155, 2467, 2494
BseMI GCAATG 3 cut(s) 777, 1011, 2637
BseMII CTCAG 9 cut(s) 44, 447, 1082, 1086, 1188, 1509, 1652, 2211, 2250
BseNI ACTGG 8 cut(s) 481, 644, 1065, 1229, 1311, 1461, 2711, 2928
BseRI GAGGAG 2 cut(s) 206, 1460
BseSI GKGCMC 2 cut(s) 2342, 2595
BseXI GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2737, 2740
BseYI CCCAGC 3 cut(s) 2324, 2444, 2526
BsgI GTGCAG 2 cut(s) 456, 1349
BshFI GGCC 7 cut(s) 151, 232, 1239, 1961, 2351, 2593, 2675
BshVI ATCGAT 2 cut(s) 2272, 2841
BsiHKAI GWGCWC 3 cut(s) 92, 1413, 2342
BsiHKCI CYCGRG 2 cut(s) 844, 1337
BsiSI CCGG 3 cut(s) 148, 1022, 1375
BslFI GGGAC 3 cut(s) 22, 251, 842
BslI CCNNNNNNNGG 3 cut(s) 1155, 2467, 2494
BsmAI GTCTC 6 cut(s) 424, 452, 511, 1423, 2090, 2253
BsmBI CGTCTC 2 cut(s) 424, 1423
BsmFI GGGAC 3 cut(s) 22, 251, 842
BsmI GAATGC 4 cut(s) 1532, 1972, 2251, 2731
BsnI GGCC 7 cut(s) 151, 232, 1239, 1961, 2351, 2593, 2675
Bso31I GGTCTC 2 cut(s) 452, 2253
BsoBI CYCGRG 2 cut(s) 844, 1337
Bsp119I TTCGAA 1 cut(s) 198
Bsp120I GGGCCC 1 cut(s) 2591
Bsp1286I GDGCHC 4 cut(s) 92, 1413, 2342, 2595
Bsp19I CCATGG 1 cut(s) 860
BspACI CCGC 3 cut(s) 1245, 1657, 2765
BspANI GGCC 7 cut(s) 151, 232, 1239, 1961, 2351, 2593, 2675
BspCNI CTCAG 9 cut(s) 43, 448, 1081, 1087, 1189, 1510, 1651, 2212, 2249
BspDI ATCGAT 2 cut(s) 2272, 2841
BspLI GGNNCC 8 cut(s) 132, 284, 479, 609, 2403, 2458, 2593, 2594
BspMAI CTGCAG 1 cut(s) 1912
BspMI ACCTGC 1 cut(s) 1321
BspT104I TTCGAA 1 cut(s) 198
BspTNI GGTCTC 2 cut(s) 452, 2253
BsrDI GCAATG 3 cut(s) 777, 1011, 2637
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 8 cut(s) 481, 644, 1065, 1229, 1311, 1461, 2711, 2928
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 6 cut(s) 189, 726, 860, 1154, 1336, 2139
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 726, 860
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 3 cut(s) 280, 810, 975
Bst4CI ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2086, 2393, 2415, 2604
Bst6I CTCTTC 3 cut(s) 2088, 2892, 2967
BstBAI YACGTR 1 cut(s) 1723
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 9 cut(s) 145, 149, 806, 1382, 1567, 1839, 1843, 2349, 2956
BstDSI CCRYGG 1 cut(s) 860
BstEII GGTNACC 1 cut(s) 947
BstENI CCTNNNNNAGG 2 cut(s) 1153, 2465
BstF5I GGATG 5 cut(s) 882, 1717, 1939, 2099, 2249
BstH2I RGCGCY 1 cut(s) 2765
BstHHI GCGC 5 cut(s) 645, 1778, 1847, 2764, 2960
BstMAI GTCTC 6 cut(s) 424, 452, 511, 1423, 2090, 2253
BstNI CCWGG 3 cut(s) 280, 810, 975
BstNSI RCATGY 5 cut(s) 147, 316, 905, 1610, 2958
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 278, 808, 973
BstSFI CTRYAG 4 cut(s) 1660, 1755, 1908, 2389
BstSLI GKGCMC 2 cut(s) 2342, 2595
BstV1I GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2737, 2740
BstV2I GAAGAC 4 cut(s) 424, 1457, 2132, 2872
BstX2I RGATCY 4 cut(s) 130, 282, 1877, 2312
BstXI CCANNNNNNTGG 4 cut(s) 279, 960, 1130, 1476
BstYI RGATCY 4 cut(s) 130, 282, 1877, 2312
Bsu15I ATCGAT 2 cut(s) 2272, 2841
BsuRI GGCC 7 cut(s) 151, 232, 1239, 1961, 2351, 2593, 2675
BsuTUI ATCGAT 2 cut(s) 2272, 2841
BtgI CCRYGG 1 cut(s) 860
BtrI CACGTC 3 cut(s) 434, 2136, 2180
BtsCI GGATG 5 cut(s) 882, 1717, 1939, 2099, 2249
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 4 cut(s) 1274, 1283, 1515, 2398
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 9 cut(s) 145, 149, 806, 1382, 1567, 1839, 1843, 2349, 2956
CfoI GCGC 5 cut(s) 645, 1778, 1847, 2764, 2960
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 7 cut(s) 910, 1333, 2401, 2456, 2591, 2592, 2674
ClaI ATCGAT 2 cut(s) 2272, 2841
Csp6I GTAC 7 cut(s) 818, 1103, 2082, 2537, 2704, 2721, 2741
CviQI GTAC 7 cut(s) 818, 1103, 2082, 2537, 2704, 2721, 2741
DraI TTTAAA 1 cut(s) 1089
DrdI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2134, 2574
DseDI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2134, 2574
EaeI YGGCCR 3 cut(s) 230, 1237, 1959
Eam1104I CTCTTC 3 cut(s) 2088, 2892, 2967
EarI CTCTTC 3 cut(s) 2088, 2892, 2967
Eco130I CCWWGG 2 cut(s) 726, 860
Eco24I GRGCYC 1 cut(s) 2595
Eco31I GGTCTC 2 cut(s) 452, 2253
Eco32I GATATC 2 cut(s) 117, 841
Eco47I GGWCC 4 cut(s) 910, 1333, 2401, 2456
Eco57I CTGAAG 2 cut(s) 1065, 1992
Eco88I CYCGRG 2 cut(s) 844, 1337
Eco91I GGTNACC 1 cut(s) 947
EcoNI CCTNNNNNAGG 2 cut(s) 1153, 2465
EcoO109I RGGNCCY 4 cut(s) 1333, 2591, 2592, 2674
EcoO65I GGTNACC 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 2483
EcoRII CCWGG 3 cut(s) 278, 808, 973
EcoRV GATATC 2 cut(s) 117, 841
EcoT14I CCWWGG 2 cut(s) 726, 860
EcoT22I ATGCAT 1 cut(s) 2251
EcoT38I GRGCYC 1 cut(s) 2595
ErhI CCWWGG 2 cut(s) 726, 860
Esp3I CGTCTC 2 cut(s) 424, 1423
FaqI GGGAC 3 cut(s) 22, 251, 842
FbaI TGATCA 1 cut(s) 2686
FblI GTMKAC 1 cut(s) 2566
Fnu4HI GCNGC 8 cut(s) 21, 450, 1563, 1570, 1779, 2726, 2729, 2765
FokI GGATG 5 cut(s) 889, 1724, 1946, 2086, 2236
FriOI GRGCYC 1 cut(s) 2595
Fsp4HI GCNGC 8 cut(s) 21, 450, 1563, 1570, 1779, 2726, 2729, 2765
FspBI CTAG 9 cut(s) 123, 393, 747, 854, 1893, 2079, 2130, 2579, 2756
FspI TGCGCA 2 cut(s) 1846, 2959
GlaI GCGC 5 cut(s) 644, 1777, 1846, 2763, 2959
GluI GCNGC 8 cut(s) 21, 450, 1563, 1570, 1779, 2726, 2729, 2765
GsaI CCCAGC 3 cut(s) 2328, 2448, 2530
GsuI CTGGAG 1 cut(s) 1764
HaeII RGCGCY 1 cut(s) 2765
HaeIII GGCC 7 cut(s) 151, 232, 1239, 1961, 2351, 2593, 2675
HapII CCGG 3 cut(s) 148, 1022, 1375
HhaI GCGC 5 cut(s) 645, 1778, 1847, 2764, 2960
Hin6I GCGC 5 cut(s) 643, 1776, 1845, 2762, 2958
HinP1I GCGC 5 cut(s) 643, 1776, 1845, 2762, 2958
HincII GTYRAC 3 cut(s) 355, 1222, 1867
HindII GTYRAC 3 cut(s) 355, 1222, 1867
HpaI GTTAAC 1 cut(s) 1867
HpaII CCGG 3 cut(s) 148, 1022, 1375
HphI GGTGA 3 cut(s) 644, 959, 1405
Hpy166II GTNNAC 8 cut(s) 355, 679, 1103, 1222, 1867, 2340, 2567, 2721
Hpy8I GTNNAC 8 cut(s) 355, 679, 1103, 1222, 1867, 2340, 2567, 2721
HpyAV CCTTC 6 cut(s) 563, 623, 1089, 1661, 1694, 2872
HpyCH4III ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2086, 2393, 2415, 2604
HpyCH4IV ACGT 6 cut(s) 274, 433, 1722, 2052, 2135, 2179
HpySE526I ACGT 6 cut(s) 274, 433, 1722, 2052, 2135, 2179
HspAI GCGC 5 cut(s) 643, 1776, 1845, 2762, 2958
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 2686
KspAI GTTAAC 1 cut(s) 1867
LmnI GCTCC 6 cut(s) 686, 1377, 1544, 1984, 2759, 2803
Lsp1109I GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2737, 2740
LweI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2258, 2431, 2838, 2967
MaeI CTAG 9 cut(s) 123, 393, 747, 854, 1893, 2079, 2130, 2579, 2756
MaeII ACGT 6 cut(s) 274, 433, 1722, 2052, 2135, 2179
MaeIII GTNAC 7 cut(s) 212, 542, 742, 947, 1063, 1287, 1302
MfeI CAATTG 2 cut(s) 1189, 2014
MflI RGATCY 4 cut(s) 130, 282, 1877, 2312
MhlI GDGCHC 4 cut(s) 92, 1413, 2342, 2595
MlsI TGGCCA 2 cut(s) 232, 1239
MluNI TGGCCA 2 cut(s) 232, 1239
MlyI GAGTC 4 cut(s) 383, 764, 1630, 2862
MmeI TCCRAC 6 cut(s) 206, 239, 380, 1147, 1457, 1689
Mox20I TGGCCA 2 cut(s) 232, 1239
Mph1103I ATGCAT 1 cut(s) 2251
MroNI GCCGGC 1 cut(s) 147
MscI TGGCCA 2 cut(s) 232, 1239
MslI CAYNNNNRTG 4 cut(s) 559, 561, 945, 2108
Msp20I TGGCCA 2 cut(s) 232, 1239
MspA1I CMGCKG 2 cut(s) 1569, 2240
MspI CCGG 3 cut(s) 148, 1022, 1375
MspR9I CCNGG 3 cut(s) 280, 810, 975
MunI CAATTG 2 cut(s) 1189, 2014
Mva1269I GAATGC 4 cut(s) 1532, 1972, 2251, 2731
MvaI CCWGG 3 cut(s) 280, 810, 975
NaeI GCCGGC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 860
NgoMIV GCCGGC 1 cut(s) 147
NlaIV GGNNCC 8 cut(s) 132, 284, 479, 609, 2403, 2458, 2593, 2594
NmeAIII GCCGAG 2 cut(s) 214, 2402
NmuCI GTSAC 3 cut(s) 947, 1063, 1287
NsbI TGCGCA 2 cut(s) 1846, 2959
NsiI ATGCAT 1 cut(s) 2251
NspI RCATGY 5 cut(s) 147, 316, 905, 1610, 2958
NspV TTCGAA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 945
PaeI GCATGC 2 cut(s) 147, 2958
PciI ACATGT 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 2316
PctI GAATGC 4 cut(s) 1532, 1972, 2251, 2731
PdiI GCCGGC 1 cut(s) 149
PfeI GAWTC 9 cut(s) 26, 242, 784, 1508, 1739, 1805, 2008, 2710, 2884
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 8 cut(s) 22, 451, 1564, 1571, 1780, 2727, 2730, 2766
PleI GAGTC 4 cut(s) 382, 763, 1630, 2862
PpsI GAGTC 4 cut(s) 382, 763, 1630, 2862
Ppu21I YACGTR 1 cut(s) 1723
PpuMI RGGWCCY 1 cut(s) 1333
PscI ACATGT 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 1333
Psp6I CCWGG 3 cut(s) 278, 808, 973
PspEI GGTNACC 1 cut(s) 947
PspFI CCCAGC 3 cut(s) 2324, 2444, 2526
PspGI CCWGG 3 cut(s) 278, 808, 973
PspN4I GGNNCC 8 cut(s) 132, 284, 479, 609, 2403, 2458, 2593, 2594
PspOMI GGGCCC 1 cut(s) 2591
PspPI GGNCC 7 cut(s) 910, 1333, 2401, 2456, 2591, 2592, 2674
PspPPI RGGWCCY 1 cut(s) 1333
PstI CTGCAG 1 cut(s) 1912
PsuI RGATCY 4 cut(s) 130, 282, 1877, 2312
PvuII CAGCTG 2 cut(s) 1569, 2240
RsaI GTAC 7 cut(s) 819, 1104, 2083, 2538, 2705, 2722, 2742
RsaNI GTAC 7 cut(s) 818, 1103, 2082, 2537, 2704, 2721, 2741
RseI CAYNNNNRTG 4 cut(s) 559, 561, 945, 2108
SatI GCNGC 8 cut(s) 21, 450, 1563, 1570, 1779, 2726, 2729, 2765
Sau96I GGNCC 7 cut(s) 910, 1333, 2401, 2456, 2591, 2592, 2674
ScaI AGTACT 1 cut(s) 2705
SchI GAGTC 4 cut(s) 383, 764, 1630, 2862
ScrFI CCNGG 3 cut(s) 280, 810, 975
SduI GDGCHC 4 cut(s) 92, 1413, 2342, 2595
SfaNI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2258, 2431, 2838, 2967
SfcI CTRYAG 4 cut(s) 1660, 1755, 1908, 2389
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 4 cut(s) 910, 1333, 2401, 2456
SmiMI CAYNNNNRTG 4 cut(s) 559, 561, 945, 2108
SmlI CTYRAG 2 cut(s) 424, 2254
SmoI CTYRAG 2 cut(s) 424, 2254
SphI GCATGC 2 cut(s) 147, 2958
SsiI CCGC 3 cut(s) 1245, 1657, 2765
SspI AATATT 1 cut(s) 2333
SspMI CTAG 9 cut(s) 123, 393, 747, 854, 1893, 2079, 2130, 2579, 2756
StyD4I CCNGG 3 cut(s) 278, 808, 973
StyI CCWWGG 2 cut(s) 726, 860
TaaI ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2086, 2393, 2415, 2604
TaiI ACGT 6 cut(s) 277, 436, 1725, 2055, 2138, 2182
TaqI TCGA 8 cut(s) 109, 198, 1032, 1114, 1251, 2272, 2285, 2841
TaqII GACCGA 2 cut(s) 476, 1602
TatI WGTACW 3 cut(s) 817, 2081, 2703
TauI GCSGC 1 cut(s) 2767
TfiI GAWTC 9 cut(s) 26, 242, 784, 1508, 1739, 1805, 2008, 2710, 2884
TscAI CASTG 4 cut(s) 1274, 1290, 1522, 2398
TseFI GTSAC 3 cut(s) 947, 1063, 1287
TseI GCWGC 7 cut(s) 20, 449, 1562, 1569, 1778, 2725, 2728
Tsp45I GTSAC 3 cut(s) 947, 1063, 1287
TspGWI ACGGA 1 cut(s) 2584
TspRI CASTG 4 cut(s) 1274, 1290, 1522, 2398
VneI GTGCAC 1 cut(s) 2338
VpaK11BI GGWCC 4 cut(s) 910, 1333, 2401, 2456
XagI CCTNNNNNAGG 2 cut(s) 1153, 2465
XapI RAATTY 8 cut(s) 329, 577, 1361, 1502, 1678, 1830, 2305, 2483
XbaI TCTAGA 1 cut(s) 2578
XceI RCATGY 5 cut(s) 147, 316, 905, 1610, 2958
XmiI GTMKAC 1 cut(s) 2566
XspI CTAG 9 cut(s) 123, 393, 747, 854, 1893, 2079, 2130, 2579, 2756
ZrmI AGTACT 1 cut(s) 2705
Zsp2I ATGCAT 1 cut(s) 2251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.