pycom10g20210

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003891
Physical Location & Seq
Reverse (-)
23166267 .. 23169463
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20210.1

Sequence Viewer

Length: 1884 bp
ATGGAACAGTTACAATTAACTGTCGAATCTCAGCAGAAAGAGGATCATTCTTCTAAGAATAACCACAAAAGTCATAACACACTTGAGGACTCTGGCAGCCCTCGAATCCGAAGGATATCAGCTAGATGGAACCCGGAGGAAGCATGCAGACCTGATATTGATGAAGCGCCTGTGTTTTATCCAACTATTGAGGAGTTCGAAGATACACTTGGTTACATAGCAAAGATCCGCCCACTGGCCGAATCTTATGGTATATGCCGGATTGTCCCTCCACCTTCCTGGACCCCTCCCTGCCCTCTTAAGGAGAAAGAGATGTGGGAACATGCCAAATTTTCTACGCGCATTCAGCAAGTTGACTTGCTTCAAAACAGAGAGGCCATGAGAAAGAAAAGTAGAAGTCGGAAGCGTAAACGAAAGAGGAACTCAAGAATGGGAGCCAGAAGGCGTTCAGAAGCTAATGCTGCTACTGAGGCTGATGAGAAGTTTGGGTTCCATTCAGGATCAGACTTTACATTTGAAGAGTTTCAGAAACATGCTGCTACTTTTAAGGAGAGTTACTTCGGAACAAAGGATGCTAAGGAGGGTTCAACTTATGGTGAAACTAAAAGCGAAACATGGCAACCCTCTGTGGAGGATATCGAAGGTGAATACTGGCGAATAGTTGAGACACCAACAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGTTTTCCTAAGGCATCATCAACCAGAAGTGATTTGGATAAGTATGCATTGTCAGGTTGGAATCTAAATAACTTCCCACGCCTGCCCGGTTCTGTACTGTGTTTTGAAGCAAGCGATATCTCAGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGCATGTTGAGGACCACCACCTCTATTCGCTAAATTATCTGCACTGGGGTGACCCAAAAGTGTGGTATGGAGTCTCTGGAAGCCATGCTACCGATTTGGAACGTACAATGAGAGCTTATTTGCCTGATCTATTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAACTATCTCCTACAGTTTTAAAGTCTGAAGGTGTACCTGTGCATCGTGCCATCCAGCATTCTGGGGAGTTTGTTCTTACCTTCCCACGGGCATATCATTCTGGATTTAATTGTGGTTTTAACTGTGCTGAGGCAGTGAACGTGGCCCCTTTTGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGTGTCGCAAGACATCAATCTCACACGACAAATTGTTACTGGGATCAGCTCAGGAAGCTGTACAGGCCCTCTGGGAACAATCAGTTCTTGGAAAGAAAACTACAAAAAATATGAGCTGGCAAAGTGTTTGTGGCAAAGATGGTCTACTCACCAGAGCAATTAAGACACGGGTGCGAATGGAGGAGGAAAGACTAGATCGTCTTCCAATTTGTATGAAGTTGAAAAAGATGGAAAGAGACTTTGATTTGAACAATGAGAGAGAATGTTTTTCTTGCTTCTATGACTTGCACTTATCTGCCTCCAGCTGCAAATGCTCTCCTGACCGATTTTCATGTCTCAAACATGCAAAGCTCCTTTGTTCATGTCACATCAATCAAAGATATGTCCTTCAACGCCACACTATCAATGAATTAAATATGCTGATTGAAGCATTGGAAGGGAAAGTAGAGGCCATAAAATTATGGGCATCCAAAGACCATGAATTAGATGGTACTGATACACGTACAGCTAAGCTGGATGAAGAACGTGGCATGCTTCATAAAAGAATCAAATCTTGTGATCCATCAGGGAAACTTCACCTTGCTGCCCGGTTTCAGAAGAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

628

Amino Acids

71.99

Weight (kDa)

6.56

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 57 - 90 3.4e-17 jmjN domain
JmjC PF02373 291 - 407 8.8e-44 JmjC domain, hydroxylase
zf-C5HC2 PF02928 514 - 564 1.2e-12 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1473
AccI GTMKAC 1 cut(s) 1420
AccII CGCG 1 cut(s) 340
AciI CCGC 1 cut(s) 229
AclWI GGATC 5 cut(s) 51, 220, 508, 1327, 1829
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 1125
AfaI GTAC 6 cut(s) 828, 1012, 1113, 1338, 1768, 1780
AfiI CCNNNNNNNGG 3 cut(s) 235, 301, 1164
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 1775
AgsI TTSAA 8 cut(s) 365, 518, 588, 839, 1498, 1525, 1667, 1703
AjnI CCWGG 1 cut(s) 278
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AleI CACNNNNGTG 1 cut(s) 954
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 4 cut(s) 659, 985, 1506, 1616
AlwI GGATC 5 cut(s) 51, 220, 508, 1327, 1829
AoxI GGCC 5 cut(s) 237, 375, 1221, 1341, 1725
ApeKI GCWGC 5 cut(s) 96, 461, 536, 1581, 1859
ApoI RAATTY 1 cut(s) 329
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 2 cut(s) 445, 522
AspLEI GCGC 2 cut(s) 169, 342
AspS9I GGNCC 4 cut(s) 282, 919, 1222, 1342
AsuC2I CCSGG 3 cut(s) 134, 819, 1864
AsuHPI GGTGA 5 cut(s) 608, 656, 968, 1417, 1844
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 2 cut(s) 282, 919
AxyI CCTNAGG 1 cut(s) 741
BanII GRGCYC 1 cut(s) 1266
BarI GAAGNNNNNNTAC 2 cut(s) 979, 1011
BbsI GAAGAC 1 cut(s) 1469
Bbv12I GWGCWC 1 cut(s) 1266
BbvCI CCTCAGC 1 cut(s) 1206
BbvI GCAGC 5 cut(s) 108, 448, 523, 1568, 1846
BccI CCATC 6 cut(s) 120, 1136, 1409, 1498, 1757, 1846
BciT130I CCWGG 1 cut(s) 280
BcnI CCSGG 3 cut(s) 134, 819, 1864
BcoDI GTCTC 4 cut(s) 659, 985, 1506, 1616
BfaI CTAG 3 cut(s) 123, 863, 1469
BfmI CTRYAG 2 cut(s) 1089, 1267
BfoI RGCGCY 1 cut(s) 170
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 5 cut(s) 97, 462, 537, 1582, 1860
BlpI GCTNAGC 1 cut(s) 1785
BlsI GCNGC 5 cut(s) 98, 463, 538, 1583, 1861
Bme1390I CCNGG 4 cut(s) 134, 280, 819, 1864
Bme18I GGWCC 2 cut(s) 282, 919
BmgT120I GGNCC 4 cut(s) 282, 919, 1222, 1342
BmiI GGNNCC 5 cut(s) 131, 284, 436, 491, 1224
BmrFI CCNGG 4 cut(s) 134, 280, 819, 1864
BmrI ACTGGG 2 cut(s) 961, 1325
BmsI GCATC 4 cut(s) 562, 755, 1129, 1751
BmuI ACTGGG 2 cut(s) 961, 1325
BpiI GAAGAC 1 cut(s) 1469
BpmI CTGGAG 1 cut(s) 1561
Bpu10I CCTNAGC 3 cut(s) 576, 1206, 1326
Bpu1102I GCTNAGC 1 cut(s) 1785
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 104, 409
BpuMI CCSGG 3 cut(s) 134, 819, 1864
BsaAI YACGTR 1 cut(s) 1778
BsaJI CCNNGG 2 cut(s) 869, 1163
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 301, 1164
Bse1I ACTGG 5 cut(s) 240, 656, 956, 1074, 1320
Bse21I CCTNAGG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 869, 1163
BseGI GGATG 5 cut(s) 577, 891, 1128, 1742, 1798
BseLI CCNNNNNNNGG 3 cut(s) 235, 301, 1164
BseMII CTCAG 5 cut(s) 44, 459, 867, 1197, 1340
BseNI ACTGG 5 cut(s) 240, 656, 956, 1074, 1320
BseRI GAGGAG 2 cut(s) 206, 1472
BseXI GCAGC 5 cut(s) 108, 448, 523, 1568, 1846
BsgI GTGCAG 1 cut(s) 932
Bsh1236I CGCG 1 cut(s) 340
BshFI GGCC 5 cut(s) 239, 377, 1223, 1343, 1727
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 4 cut(s) 134, 259, 819, 1864
BslFI GGGAC 2 cut(s) 251, 851
BslI CCNNNNNNNGG 3 cut(s) 235, 301, 1164
BsmAI GTCTC 4 cut(s) 659, 985, 1506, 1616
BsmFI GGGAC 2 cut(s) 251, 851
BsmI GAATGC 2 cut(s) 342, 1135
BsnI GGCC 5 cut(s) 239, 377, 1223, 1343, 1727
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 1266
Bsp1407I TGTACA 1 cut(s) 1336
Bsp143I GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
Bsp1720I GCTNAGC 1 cut(s) 1785
Bsp19I CCATGG 1 cut(s) 869
BspACI CCGC 1 cut(s) 229
BspANI GGCC 5 cut(s) 239, 377, 1223, 1343, 1727
BspCNI CTCAG 5 cut(s) 43, 460, 866, 1198, 1339
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 5 cut(s) 131, 284, 436, 491, 1224
BspPI GGATC 5 cut(s) 51, 220, 508, 1327, 1829
BspT104I TTCGAA 1 cut(s) 198
BspTI CTTAAG 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 1336
BsrI ACTGG 5 cut(s) 240, 656, 956, 1074, 1320
BssECI CCNNGG 2 cut(s) 869, 1163
BssMI GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
BssT1I CCWWGG 1 cut(s) 869
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 6 cut(s) 9, 22, 831, 1093, 1202, 1271
Bst6I CTCTTC 2 cut(s) 513, 1868
BstAFI CTTAAG 1 cut(s) 299
BstAUI TGTACA 1 cut(s) 1336
BstBAI YACGTR 1 cut(s) 1778
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 5 cut(s) 145, 815, 844, 1394, 1808
BstDEI CTNAG 9 cut(s) 30, 54, 468, 576, 741, 853, 1206, 1326, 1785
BstDSI CCRYGG 2 cut(s) 869, 1163
BstEII GGTNACC 1 cut(s) 956
BstF5I GGATG 5 cut(s) 577, 891, 1128, 1742, 1798
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 169, 342
BstKTI GATC 7 cut(s) 46, 228, 503, 1036, 1322, 1474, 1837
BstMAI GTCTC 4 cut(s) 659, 985, 1506, 1616
BstMBI GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
BstMWI GCNNNNNNNGC 6 cut(s) 346, 461, 470, 1331, 1340, 1587
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 6 cut(s) 147, 326, 536, 914, 1622, 1810
BstPI GGTNACC 1 cut(s) 956
BstSCI CCNGG 4 cut(s) 132, 278, 817, 1862
BstSFI CTRYAG 2 cut(s) 1089, 1267
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 5 cut(s) 108, 448, 523, 1568, 1846
BstV2I GAAGAC 1 cut(s) 1469
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 3 cut(s) 279, 969, 1139
BstYI RGATCY 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 741
BsuRI GGCC 5 cut(s) 239, 377, 1223, 1343, 1727
BtgI CCRYGG 2 cut(s) 869, 1163
BtsCI GGATG 5 cut(s) 577, 891, 1128, 1742, 1798
BtsI GCAGTG 2 cut(s) 1218, 1283
BtsIMutI CAGTG 5 cut(s) 233, 949, 1218, 1276, 1283
Cac8I GCNNGC 5 cut(s) 145, 815, 844, 1394, 1808
CfoI GCGC 2 cut(s) 169, 342
Cfr13I GGNCC 4 cut(s) 282, 919, 1222, 1342
Csp6I GTAC 6 cut(s) 827, 1011, 1112, 1337, 1767, 1779
CviQI GTAC 6 cut(s) 827, 1011, 1112, 1337, 1767, 1779
DdeI CTNAG 9 cut(s) 30, 54, 468, 576, 741, 853, 1206, 1326, 1785
DpnI GATC 7 cut(s) 45, 227, 502, 1035, 1321, 1473, 1836
DpnII GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
DraI TTTAAA 1 cut(s) 1098
DrdI GACNNNNNNGTC 1 cut(s) 1473
DseDI GACNNNNNNGTC 1 cut(s) 1473
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 513, 1868
EarI CTCTTC 2 cut(s) 513, 1868
EciI GGCGGA 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 1264
Eco130I CCWWGG 1 cut(s) 869
Eco24I GRGCYC 1 cut(s) 1266
Eco32I GATATC 3 cut(s) 117, 637, 850
Eco47I GGWCC 2 cut(s) 282, 919
Eco53kI GAGCTC 1 cut(s) 1264
Eco57I CTGAAG 1 cut(s) 1125
Eco81I CCTNAGG 1 cut(s) 741
Eco91I GGTNACC 1 cut(s) 956
EcoICRI GAGCTC 1 cut(s) 1264
EcoO109I RGGNCCY 1 cut(s) 1342
EcoO65I GGTNACC 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 278
EcoRV GATATC 3 cut(s) 117, 637, 850
EcoT14I CCWWGG 1 cut(s) 869
EcoT22I ATGCAT 1 cut(s) 781
EcoT38I GRGCYC 1 cut(s) 1266
ErhI CCWWGG 1 cut(s) 869
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 2 cut(s) 251, 851
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 5 cut(s) 97, 462, 537, 1582, 1860
FokI GGATG 5 cut(s) 584, 898, 1115, 1729, 1805
FriOI GRGCYC 1 cut(s) 1266
Fsp4HI GCNGC 5 cut(s) 97, 462, 537, 1582, 1860
FspBI CTAG 3 cut(s) 123, 863, 1469
GlaI GCGC 2 cut(s) 168, 341
GluI GCNGC 5 cut(s) 97, 462, 537, 1582, 1860
GsuI CTGGAG 1 cut(s) 1561
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 5 cut(s) 239, 377, 1223, 1343, 1727
HapII CCGG 4 cut(s) 134, 259, 819, 1864
HhaI GCGC 2 cut(s) 169, 342
Hin6I GCGC 2 cut(s) 167, 340
HinP1I GCGC 2 cut(s) 167, 340
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 7 cut(s) 26, 89, 105, 242, 793, 978, 1821
HpaII CCGG 4 cut(s) 134, 259, 819, 1864
HphI GGTGA 5 cut(s) 608, 656, 968, 1417, 1844
Hpy166II GTNNAC 6 cut(s) 355, 410, 694, 1112, 1216, 1421
Hpy188I TCNGA 8 cut(s) 110, 402, 451, 505, 528, 563, 1105, 1872
Hpy188III TCNNGA 7 cut(s) 426, 498, 855, 984, 1179, 1328, 1595
Hpy8I GTNNAC 6 cut(s) 355, 410, 694, 1112, 1216, 1421
HpyAV CCTTC 9 cut(s) 105, 285, 435, 635, 1100, 1168, 1673, 1706, 1872
HpyCH4III ACNGT 6 cut(s) 9, 22, 831, 1093, 1202, 1271
HpyCH4IV ACGT 4 cut(s) 1009, 1218, 1777, 1801
HpyCH4V TGCA 8 cut(s) 147, 779, 949, 1120, 1256, 1564, 1584, 1622
HpyF10VI GCNNNNNNNGC 6 cut(s) 346, 461, 470, 1331, 1340, 1587
HpyF3I CTNAG 9 cut(s) 30, 54, 468, 576, 741, 853, 1206, 1326, 1785
HpySE526I ACGT 4 cut(s) 1009, 1218, 1777, 1801
HspAI GCGC 2 cut(s) 167, 340
Kzo9I GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
LmnI GCTCC 3 cut(s) 434, 701, 1632
Lsp1109I GCAGC 5 cut(s) 108, 448, 523, 1568, 1846
LweI GCATC 4 cut(s) 562, 755, 1129, 1751
MaeI CTAG 3 cut(s) 123, 863, 1469
MaeII ACGT 4 cut(s) 1009, 1218, 1777, 1801
MaeIII GTNAC 7 cut(s) 9, 212, 554, 956, 1072, 1311, 1640
MalI GATC 7 cut(s) 45, 227, 502, 1035, 1321, 1473, 1836
MboI GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
MboII GAAGA 6 cut(s) 42, 212, 530, 888, 1469, 1808
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 1266
MlyI GAGTC 2 cut(s) 83, 987
MmeI TCCRAC 3 cut(s) 206, 380, 770
Mph1103I ATGCAT 1 cut(s) 781
MroXI GAANNNNTTC 2 cut(s) 445, 522
MseI TTAA 8 cut(s) 17, 300, 546, 1097, 1185, 1197, 1437, 1688
MslI CAYNNNNRTG 1 cut(s) 954
MspA1I CMGCKG 1 cut(s) 1581
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 4 cut(s) 134, 259, 819, 1864
MspR9I CCNGG 4 cut(s) 134, 280, 819, 1864
Mva1269I GAATGC 2 cut(s) 342, 1135
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 6 cut(s) 346, 461, 470, 1331, 1340, 1587
NciI CCSGG 3 cut(s) 134, 819, 1864
NcoI CCATGG 1 cut(s) 869
NdeII GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
NlaIV GGNNCC 5 cut(s) 131, 284, 436, 491, 1224
NmuCI GTSAC 3 cut(s) 956, 1072, 1640
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 6 cut(s) 147, 326, 536, 914, 1622, 1810
NspV TTCGAA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 954
PaeI GCATGC 2 cut(s) 147, 1810
PctI GAATGC 2 cut(s) 342, 1135
PdmI GAANNNNTTC 2 cut(s) 445, 522
PfeI GAWTC 5 cut(s) 26, 105, 242, 793, 1821
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 5 cut(s) 98, 463, 538, 1583, 1861
PleI GAGTC 2 cut(s) 83, 986
PpsI GAGTC 2 cut(s) 83, 986
Ppu21I YACGTR 1 cut(s) 1778
Psp124BI GAGCTC 1 cut(s) 1266
Psp6I CCWGG 1 cut(s) 278
PspEI GGTNACC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 5 cut(s) 131, 284, 436, 491, 1224
PspPI GGNCC 4 cut(s) 282, 919, 1222, 1342
PsuI RGATCY 1 cut(s) 225
PvuII CAGCTG 1 cut(s) 1581
RsaI GTAC 6 cut(s) 828, 1012, 1113, 1338, 1768, 1780
RsaNI GTAC 6 cut(s) 827, 1011, 1112, 1337, 1767, 1779
RseI CAYNNNNRTG 1 cut(s) 954
SacI GAGCTC 1 cut(s) 1266
SaqAI TTAA 8 cut(s) 17, 300, 546, 1097, 1185, 1197, 1437, 1688
SatI GCNGC 5 cut(s) 97, 462, 537, 1582, 1860
Sau3AI GATC 7 cut(s) 43, 225, 500, 1033, 1319, 1471, 1834
Sau96I GGNCC 4 cut(s) 282, 919, 1222, 1342
SchI GAGTC 2 cut(s) 83, 987
ScrFI CCNGG 4 cut(s) 134, 280, 819, 1864
SduI GDGCHC 1 cut(s) 1266
SfaNI GCATC 4 cut(s) 562, 755, 1129, 1751
SfcI CTRYAG 2 cut(s) 1089, 1267
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 2 cut(s) 282, 919
SmiMI CAYNNNNRTG 1 cut(s) 954
SmlI CTYRAG 3 cut(s) 83, 299, 424
SmoI CTYRAG 3 cut(s) 83, 299, 424
SphI GCATGC 2 cut(s) 147, 1810
SsiI CCGC 1 cut(s) 229
SspMI CTAG 3 cut(s) 123, 863, 1469
SstI GAGCTC 1 cut(s) 1266
StyD4I CCNGG 4 cut(s) 132, 278, 817, 1862
StyI CCWWGG 1 cut(s) 869
TaaI ACNGT 6 cut(s) 9, 22, 831, 1093, 1202, 1271
TaiI ACGT 4 cut(s) 1012, 1221, 1780, 1804
TaqI TCGA 5 cut(s) 24, 103, 198, 639, 1041
TaqII GACCGA 1 cut(s) 1614
TatI WGTACW 2 cut(s) 826, 1336
TfiI GAWTC 5 cut(s) 26, 105, 242, 793, 1821
Tru1I TTAA 8 cut(s) 17, 300, 546, 1097, 1185, 1197, 1437, 1688
Tru9I TTAA 8 cut(s) 17, 300, 546, 1097, 1185, 1197, 1437, 1688
TscAI CASTG 5 cut(s) 240, 956, 1218, 1276, 1283
TseFI GTSAC 3 cut(s) 956, 1072, 1640
TseI GCWGC 5 cut(s) 96, 461, 536, 1581, 1859
Tsp45I GTSAC 3 cut(s) 956, 1072, 1640
TspRI CASTG 5 cut(s) 240, 956, 1218, 1276, 1283
Vha464I CTTAAG 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 282, 919
XapI RAATTY 1 cut(s) 329
XceI RCATGY 6 cut(s) 147, 326, 536, 914, 1622, 1810
XcmI CCANNNNNNNNNTGG 2 cut(s) 763, 1760
XmiI GTMKAC 1 cut(s) 1420
XmnI GAANNNNTTC 2 cut(s) 445, 522
XspI CTAG 3 cut(s) 123, 863, 1469
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.